Article
Biology
Ruchi Roy, Janet Zayas, Sunil K. Singh, Kaylee Delgado, Stephen J. Wood, Mohamed F. Mohamed, Dulce M. Frausto, Yasmeen A. Albalawi, Thea P. Price, Ricardo Estupinian, Eileena F. Giurini, Timothy M. Kuzel, Andrew Zloza, Jochen Reiser, Sasha H. Shafikhani
Summary: Infection is a common complication that hinders the healing process in diabetic wounds. Research has found that exposure to glucose impairs chemotaxis signaling in diabetic neutrophils, leading to reduced chemotaxis and delayed migration, making diabetic wounds more susceptible to infection. However, the pro-inflammatory cytokine CCL3 can override the impaired signaling and improve infection control, as well as stimulate healing in diabetic wounds.
Article
Microbiology
Adrienne R. Kambouris, Jerod A. Brammer, Holly Roussey, Chixiang Chen, Alan S. Cross
Summary: The combination of burn state and infection affects gene expression in both the host and pathogen, increasing the propensity for infection. Inhibiting IL-10 signaling or co-administering arginine can improve survival rates in a burn and infection model.
Review
Immunology
Gabriel Moran, Benjamin Uberti, John Quiroga
Summary: This review discusses the importance of neutrophil extracellular traps (NETs) in health and disease, particularly in lung diseases. The formation of NETs is closely associated with metabolic pathways and may impact immune functions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Santosh Pandit, Mengyue Li, Yanyan Chen, Shadi Rahimi, Vrss Mokkapati, Alessandra Merlo, August Yurgens, Ivan Mijakovic
Summary: The study presented a simple sensor based on pristine, non-functionalized graphene, capable of detecting and distinguishing different bacterial species. The sensor's fabrication is straightforward, can be easily miniaturized, and is effective in cases where precise species identification is not required.
Article
Microbiology
Xuerui Bao, Ellen Goeteyn, Aurelie Crabbe, Tom Coenye
Summary: D,L-malate enhances ciprofloxacin activity against P. aeruginosa, improving antibiotic therapy in infection environments. The choice of model system influences the outcome of the experiment, emphasizing the importance of using models that closely mimic the in vivo environment. This study confirms the potential of D,L-malate to enhance ciprofloxacin activity against P. aeruginosa-associated infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Immunology
Rajalakshmy Ayilam Ramachandran, Andrew Lemoff, Danielle M. Robertson
Summary: Pseudomonas aeruginosa keratitis occurs in individuals who have suffered trauma, in immunocompromised patients, and in otherwise healthy individuals who wear contact lenses. It is characterized by a light-blocking infiltrate and is the most severe complication associated with contact lens wear, potentially leading to vision loss.
INFECTION AND IMMUNITY
(2023)
Article
Immunology
Juliana Bernardi Aggio, Barbara Nery Porto, Claudia Nunes Duarte dos Santos, Ana Luiza Pamplona Mosimann, Pryscilla Fanini Wowk
Summary: This study found that human neutrophils are not permissive to Zika virus infection, but they may participate in shaping Zika virus infection in other target cells by being involved in the infected environment.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Eleonora Secchi, Giovanni Savorana, Alessandra Vitale, Leo Eberl, Roman Stocker, Roberto Rusconi
Summary: It has been found that the mechanical stability of Pseudomonas aeruginosa streamers mainly relies on the presence of extracellular DNA (eDNA) and the exopolysaccharide Pel. Controlling the composition of streamers can effectively control the formation of biofilm structures.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Kara R. Eichelberger, Saikat Paul, Brian M. Peters, James E. Cassat
Summary: This review highlights the impact of Candida albicans-bacteria interactions on healthy polymicrobial community structure, host immune responses, and microbial pathogenesis, and discusses the significance of these interactions in discovering novel antivirulence compounds for fungal infections.
TRENDS IN MICROBIOLOGY
(2023)
Article
Immunology
Shubhanshi Trivedi, Allie H. Grossmann, Owen Jensen, Mark J. Cody, Taylor A. Wahlig, Paula Hayakawa Serpa, Charles Langelier, Kristi J. Warren, Christian C. Yost, Daniel T. Leung
Summary: The study found that intestinal infection impacts lung innate immune responses, especially neutrophil characteristics, potentially increasing susceptibility to secondary pneumonia. Mice with prior intestinal infection had higher lung bacterial burden and inflammation when challenged with Klebsiella pneumoniae.
OPEN FORUM INFECTIOUS DISEASES
(2021)
Article
Immunology
Nina Pennetzdorfer, Medeea C. Popescu, Naomi L. Haddock, Fannie Dupuy, Gernot Kaber, Aviv Hargil, Patrik K. Johansson, Annika Enejder, Paul L. Bollyky
Summary: Pseudomonas aeruginosa, a major human pathogen, can effectively colonize the airways of patients with cystic fibrosis. This study found that the bacteriophage Pf4 produced by P. aeruginosa modifies the innate immune response to P. aeruginosa infections via TLR3 signaling. The phage dampens inflammatory responses to bacterial endotoxin by attaching to bacterial vesicles, potentially reducing inflammation at sites of bacterial colonization or infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Calvin K. Lee, William C. Schmidt, Shanice S. Webster, Jonathan W. Chen, George A. O'Toole, Gerard C. L. Wong
Summary: This study reveals that changes in c-di-GMP levels in bacterial biofilms are not the sole factor determining surface commitment, as oscillations in c-di-GMP levels can propagate across generations. Additionally, the study demonstrates that modulation of c-di-GMP signals and cooperative surface commitment can be influenced by varying the interaction strength between PilO and SadC.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Esther Shmidov, Ilana Lebenthal-Loinger, Shira Roth, Sarit Karako-Lampert, Itzhak Zander, Sivan Shoshani, Amos Danielli, Ehud Banin
Summary: The functions of bacterial TA systems are still disputed and poorly understood. This study sheds light on potential functions of bacterial TA systems. The novel type II TA system PrrT/A in Pseudomonas aeruginosa is found to regulate biofilm formation, motility, prophage induction, bacteriophage production, and aminoglycoside resistance, providing new insights into the diverse roles and impacts of TA systems in bacteria.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Bianca L. Ferreira, Ivan Ramirez-Moral, Natasja A. Otto, Reinaldo Salomao, Alex F. de Vos, Tom van der Poll
Summary: During acute P. aeruginosa pneumonia, the PPAR-gamma agonist pioglitazone exerts a selective proinflammatory effect on bronchial epithelial cells, possibly by enhancing intracellular glycolysis.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Konstantin Shatalin, Ashok Nuthanakanti, Abhishek Kaushik, Dmitry Shishov, Alla Peselis, Ilya Shamovsky, Bibhusita Pani, Mirna Lechpammer, Nikita Vasilyev, Elena Shatalina, Dmitri Rebatchouk, Alexander Mironov, Peter Fedichev, Alexander Serganov, Evgeny Nudler
Summary: The emergence of resistance to clinical antibiotics has prompted the development of a new antimicrobial strategy targeting the bacterial hydrogen sulfide (H2S) defense system. Inhibitors of the bacterial cystathionine g-lyase (CSE) have been found to enhance the effectiveness of bactericidal antibiotics against major human pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa. These inhibitors also disrupt bacterial tolerance, reduce biofilm formation, and decrease the number of persister bacteria surviving antibiotic treatment.
Article
Cardiac & Cardiovascular Systems
Alexis Slama, Mohamed Zaatar, Muhittin Demir, Oezlem Okumus, Stefan Mattheis, Sandra Kampe, Kaid Darwiche, Stephan Lang, Clemens Aigner
Summary: The study found that in carefully selected patients, tracheal or laryngotracheal resection after previous interventions provides comparable outcomes to primary surgery.
THORACIC AND CARDIOVASCULAR SURGEON
(2022)
Article
Biochemistry & Molecular Biology
Sarina Bense, Julius Witte, Matthias Preusse, Michal Koska, Lorena Pezoldt, Astrid Droge, Oliver Hartmann, Mathias Musken, Julia Schulze, Timm Fiebig, Heike Baehre, Sebastian Felgner, Andreas Pich, Susanne Haeussler
Summary: C-di-GMP signaling directly affects bacterial behavior by influencing protein functionality, and it can also have a global impact on gene transcription or translation. This study investigated the effects of changes in intracellular c-di-GMP levels on gene expression and protein production in Pseudomonas aeruginosa, and found that an increase in c-di-GMP levels caused a switch in phenotype before any changes in gene transcription or protein abundance were observed.
MOLECULAR MICROBIOLOGY
(2022)
Article
Neurosciences
Grzegorz Wiera, Patrycja Brzdak, Anna Maria Lech, Katarzyna Lebida, Jadwiga Jablonska, Przemyslaw Gmerek, Jerzy W. Mozrzymas
Summary: This study provides the first evidence that integrins play a key role in regulating the endogenous modulatory mechanisms of GABAergic inhibition and plasticity in the hippocampus.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Audiology & Speech-Language Pathology
Laura Christine Holtmann, Amadea Strahlenbach, Stefan Hans, Stephan Lang, Diana Arweiler-Harbeck
Summary: The medial olivocochlear reflex (MOCR) may affect bimodal hearing in some individuals, but it is not observable in all cases. Threshold changes observed in acoustic brainstem response (ABR) measurements were reproducible in a specific subgroup, potentially due to a reactivated MOCR.
AUDIOLOGY AND NEURO-OTOLOGY
(2023)
Article
Otorhinolaryngology
Benedikt Hoeing, L. Wittig, L. Weber, B. A. Stuck, S. Mattheis, T. Hussain, S. Lang, J. M. Theysohn, Y. Li, S. Hansen
Summary: This retrospective study aimed to investigate the use of abdominal ultrasound as a staging procedure in head and neck squamous cell carcinoma (HNSCC). Out of 426 patients who received abdominal ultrasound, 7% were suspected of metastasis, with the majority being liver masses. The study found that abdominal ultrasound had a negative predictive value of 99.03% and a positive predictive value of 5.88% in HNSCC patients with N2/N3 cervical lymph node status.
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
(2023)
Article
Otorhinolaryngology
Eric Deuss, Saskia Schieder, Stephan Lang, Stefan Mattheis, Orlando Guntinas-Lichius, Moritz Friedo Meyer
Summary: This survey aimed to assess the diagnostic workup and therapeutic strategies for salivary gland diseases in German otorhinolaryngology departments. The results showed diversity in the diagnosis and treatment methods, with variations in different locations.
Article
Oncology
Luiza Martins Nascentes Melo, Dayana Herrera-Rios, Daniel Hinze, Stefanie Loeffek, Irem Oezel, Roberta Turiello, Juliane Klein, Sonia Leonardelli, Isa-Vanessa Westedt, Yahya Al-Matary, Sara Egea-Rodriguez, Alexandra Brenzel, Maja Bau, Antje Sucker, Eva Hadaschik, Florian Wirsdoerfer, Helmut Hanenberg, Niklas Uhlenbrock, Daniel Rauh, Joanna Pozniak, Florian Rambow, Jean-Christophe Marine, Maike Effern, Nicole Glodde, Dirk Schadendorf, Jadwiga Jablonska, Michael Hoelzel, Iris Helfrich
Summary: This study identified HSD11B1 as a negative feedback mechanism that limits the efficacy of PD-1 blockade, and showed that HSD11B1 inhibitors can enhance the effectiveness of immunotherapy for melanoma. Additionally, high levels of HSD11B1 were associated with poor responses to immune checkpoint inhibitors in patients with advanced melanoma.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Microbiology
Kathrin Alpers, Elisabeth Vatareck, Lothar Groebe, Mathias Muesken, Maren Scharfe, Susanne Haeussler, Jurgen Tomasch
Summary: Pseudomonas aeruginosa undergoes a transition from fast growth with overlapping cell cycles to sustained slow growth with non-overlapping cell cycles when cultivated under standard laboratory conditions. This transition is characterized by sequential changes in transcriptional activity along the chromosome and a shift in quorum sensing activity. RNA sequencing of subpopulations identified distinct gene expression patterns for replicating and nonreplicating cells.
Article
Oncology
Leah S. Stockman, Daniel A. Gundersen, Ajami Gikandi, Ruth N. Akindele, Ludmila Svoboda, Sarah Pohl, Mark R. Drews, Christopher S. Lathan
Summary: Cancer disparities among historically marginalized populations are addressed through a clinical outreach program that integrates cancer diagnostic services and patient navigation within a community-based primary healthcare setting. The program aims to expedite cancer diagnoses and establish collaborative care between oncology specialists and primary care providers. The majority of patients self-identified as Black and Hispanic, with a high prevalence of comorbid health conditions and financial distress.
JCO ONCOLOGY PRACTICE
(2023)
Article
Biochemical Research Methods
Debbie Pankratz, Nicolas Oswaldo Gomez, Agnes Nielsen, Ayten Mustafayeva, Melisa Guer, Fabian Arce-Rodriguez, Pablo Ivan Nikel, Susanne Haeussler, Alejandro Arce-Rodriguez
Summary: Much of our current understanding of microbiology is based on the application of genetic engineering procedures. In this article, a method for precise and efficient engineering of Pseudomonas aeruginosa is presented. This method utilizes targeted double-strand DNA breaks and efficient recombinase to introduce a range of genetic modifications, reducing the time required for these manipulations.
Article
Immunology
Kristina Zec, Stephanie Thiebes, Jenny Bottek, Devon Siemes, Philippa Spangenberg, Duc Viet Trieu, Nils Kirstein, Nirojah Subramaniam, Robin Christ, Diana Klein, Verena Jendrossek, Maria Loose, Florian Wagenlehner, Jadwiga Jablonska, Thilo Bracht, Barbara Sitek, Bettina Budeus, Ludger Klein-Hitpass, Dirk Theegarten, Olga Shevchuk, Daniel R. Engel
Summary: This study provides novel insights into the molecular adaptations of alveolar macrophages (AM) upon lung infection with Streptococcus pneumoniae. The results reveal a strong neutrophil-associated proteomic signature in AM, and identify CD11b as a central molecular hub influencing neutrophil recruitment, activation, and migration.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Luisa Tengler, Julia Schuetz, Moritz Tiedtke, Jadwiga Jablonska, Marie-Nicole Theodoraki, Katja Nitschke, Christel Weiss, Elena Seiz, Annette Affolter, Frederic Jungbauer, Anne Lammert, Nicole Rotter, Sonja Ludwig
Summary: Through the study of plasma small extracellular vesicles (sEVs) in patients with head and neck cancer (HNC), it was found that these vesicles contain proteins that inhibit angiogenesis, thereby suppressing the angiogenic properties of endothelial cells. However, sEVs from advanced-stage HNC patients actually promote angiogenesis. Therefore, tumor-derived sEVs may increase the likelihood of angiogenesis in HNC patients.
MOLECULAR MEDICINE
(2023)
Article
Infectious Diseases
Janne G. Thoming, Susanne Haeussler
Summary: This chapter reviews recent developments in transcriptional profiling of Pseudomonas aeruginosa infections and explains how advanced RNA-seq technology can provide new insights into the establishment and maintenance of these infections. It also discusses the use of large transcriptome datasets from clinical isolates to gain a greater understanding of bacterial adaptation during the infection process. Global genotype phenotype correlation studies have shed light on new evolutionary pathways and strategies used by P. aeruginosa to build a biofilm.
PSEUDOMONAS AERUGINOSA: Biology, Pathogenesis and Control Strategies
(2022)