Article
Biochemical Research Methods
Jingshu Li, Ye Cao, Yun Yang, Huanhuan Ma, Jing Zhao, Yaoyang Zhang, Nan Liu
Summary: Protein lysine acetylation is a dynamic PTM that regulates various cellular events, including aging. This study focuses on GCN5 and explores its acetylation substrates in vivo using Drosophila. The results reveal an increase in protein acetylation with age, potentially triggered by the progressive increase in endogenous fly Gcn5 expression. Overall, this study expands our understanding of GCN5 substrates and their contribution to the aging acetylome.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Oncology
Xiaoqiang Chai, Jianfei Guo, Ruizhao Dong, Xuan Yang, Chao Deng, Chuanyuan Wei, JiaJie Xu, Weiyu Han, Jiacheng Lu, Chao Gao, Dongmei Gao, Cheng Huang, Aiwu Ke, Shuangqi Li, Huanping Li, Yingming Tian, Zhongkai Gu, Shuxian Liu, Hang Liu, Qilong Chen, Feng Liu, Jian Zhou, Jia Fan, Guoming Shi, Feizhen Wu, Jiabin Cai
Summary: This study characterized the role of lysine acetylation in hepatocellular carcinoma, revealing multiple acetylation sites and their changes in different tissues. The study also found a correlation between acetylation levels and prognosis in HCC patients.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Charlotte M. Francois, Thomas Pihl, Marion Dunoyer de Segonzac, Chloe Herault, Bruno Hudry
Summary: This study reveals the interaction between cellular metabolism and differentiation, with circulating citrate regulating sperm production through specific protein post-translational modification.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Aniko Farago, Nora Zsindely, Anita Farkas, Alexandra Neller, Fruzsina Siagi, Marton Richard Szabo, Tamas Csont, Laszlo Bodai
Summary: Huntington's Disease is a fatal neurodegenerative disorder caused by the expansion of a polyglutamine-coding CAG repeat in the Huntingtin gene. One of the main causes of neurodegeneration in HD is transcriptional dysregulation, which is partly caused by the inhibition of histone acetyltransferase enzymes. Increasing the activity of specific HATs or inhibiting histone deacetylase enzymes can alleviate HD pathology. This study suggests that Gcn5-dependent acetylation of H3.3K14 may be an important epigenetic contributor to HD pathology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Subba Reddy Palli
Summary: Modifications to DNA and core histones play crucial roles in chromatin organization and gene expression, affecting various biological processes. Studies have identified genes encoding enzymes that modify core histones in multiple insect genomes, with some enzymes contributing to post-embryonic development. Research on epigenetic modifiers could help identify inhibitors that may be used to control pests and disease vectors.
CURRENT OPINION IN INSECT SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Yoshihiro Matsumura, Timothy F. Osborne, Juro Sakai
Summary: Adipocytes are essential for maintaining energy homeostasis in the body. Different types of adipocytes regulate energy storage and expenditure through dynamic control by environmental cues. Recent studies have shown that environmental signals regulate gene expression in adipocytes through the interaction between transcription factors and epigenomic chromatin regulators.
JOURNAL OF BIOCHEMISTRY
(2022)
Review
Cell Biology
Fanyun Kong, Lihong Ma, Xing Wang, Hongjuan You, Kuiyang Zheng, Renxian Tang
Summary: Research has found that lysine acetylation plays a role in modulating molecules involved in EMT, including marker proteins, transcription factors, and signal transduction molecules. Histone acetylation functions through epigenetic regulation, while non-histone acetylation is a post-translational modification, both contributing to the control of EMT. Inhibitors targeting KATs and KDACs have potential therapeutic value in cancer treatment by suppressing EMT and tumor development.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Jin Song, Julia A. VanBuskirk, Shannath L. Merbs
Summary: This study reveals an important role of epigenetic regulation in controlling opsin expression, and demonstrates this through experiments using epigenetic modifiers in cell cultures and mouse retinal explants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Genetics & Heredity
Merrick S. Fallah, Dora Szarics, Clara M. Robson, James H. Eubanks
Summary: Epigenetic processes play a critical role in regulating gene expression patterns in neurodevelopment, including the dynamic network of post-translational histone modifications. Mutations in histone-modifying enzymes can disrupt this system and lead to developmental consequences. This review examines neurodevelopmental conditions associated with histone methylation and acetylation, discussing clinical phenotypes, genetic bases, and model systems for studying pathological mechanisms.
FRONTIERS IN GENETICS
(2021)
Article
Biology
Kanishk Jain, Matthew R. Marunde, Jonathan M. Burg, Susan L. Gloor, Faith M. Joseph, Karl F. Poncha, Zachary B. Gillespie, Keli L. Rodriguez, Irina K. Popova, Nathan W. Hall, Anup Vaidya, Sarah A. Howard, Hailey F. Taylor, Laylo Mukhsinova, Ugochi C. Onuoha, Emily F. Patteson, Spencer W. Cooke, Bethany C. Taylor, Ellen N. Weinzapfel, Marcus A. Cheek, Matthew J. Meiners, Geoffrey C. Fox, Kevin E. W. Namitz, Martis W. Cowles, Krzysztof Krajewski, Zu-Wen Sun, Michael S. Cosgrove, Nicolas L. Young, Michael-Christopher Keogh, Brian D. Strahl
Summary: This study reveals that H3 tail acetylation promotes nucleosomal accessibility to H3K4 methyl readers and writers, resolving the long-standing question of why H3K4me3 levels are coupled with H3 acetylation.
Article
Cell Biology
Munerah Hamed, Jihong Chen, Qiao Li
Summary: Dystroglycan, a key component for muscle membrane integrity, is regulated by retinoid X receptor signaling and plays a crucial role in myoblast differentiation. It is a target gene of the muscle master regulator MyoD and its expression is influenced by residue-specific histone acetylation. Our study provides new insights into the regulation and function of dystroglycan in myoblasts and suggests potential therapeutic approaches for muscle pathology.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Medicinal
Katherine L. Jones, Dominic M. Beaumont, Sharon G. Bernard, Rino A. Bit, Simon P. Campbell, Chun-wa Chung, Leanne Cutler, Emmanuel H. Demont, Kate Dennis, Laurie Gordon, James R. Gray, Michael Haase, Antonia J. Lewis, Scott McCleary, Darren J. Mitchell, Susanne M. Moore, Nigel Parr, Olivia J. Robb, Nicholas Smithers, Peter E. Soden, Colin J. Suckling, Simon Taylor, Ann L. Walker, Robert J. Watson, Rab K. Prinjha
Summary: This study details the optimization process of the in vivo tool molecule I-BET151 towards I-BET282E, a molecule with properties suitable for clinical progression, in order to reduce the risk of compound attrition due to related toxicity findings.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Nofar Harpaz, Tamir Mittelman, Olga Beresh, Ofir Griess, Noa Furth, Tomer-Meir Salame, Roni Oren, Liat Fellus-Alyagor, Alon Harmelin, Sanda Alexandrescu, Joana Graca Marques, Mariella G. Filbin, Guy Ron, Efrat Shema
Summary: This study adapted CyTOF to analyze histone modifications in DIPG tumor cells and identified two epigenetically distinct subpopulations. These subpopulations were consistent across different tumor lines, and showed differences in proliferation capacity and expression of stem cell and differentiation markers. The study also demonstrated potential interactions between histone modifications and epigenetic alterations during the cell cycle.
Article
Engineering, Environmental
Huanliang Liu, Lijie Tian, Man Qu, Dayong Wang
Summary: Using Caenorhabditis elegans as a model, the study demonstrates that CBP-1 mediates protective response to polystyrene nanoparticles (PS-NPs) toxicity by regulating various signaling pathways in intestinal, neuronal, and germline cells.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Immunology
Chao Yang, Dan Li, Dehong Teng, Yueru Zhou, Lei Zhang, Zhangfeng Zhong, Guan-Jun Yang
Summary: This review discusses the research on the pathogenesis of rheumatoid arthritis (RA) in the past five years based on epigenetic modifications, providing new methods and strategies for the diagnosis and treatment of this disease.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Tiffiney R. Hartman, Erin M. Ventresca, Anthony Hopkins, Daniel Zinshteyn, Tanu Singh, Jenny A. O'Brien, Benjamin C. Neubert, Matthew G. Hartman, Heather K. Schofield, Kevin P. Stavrides, Danielle E. Talbot, Devon J. Riggs, Caroline Pritchard, Alana M. O'Reilly
Article
Genetics & Heredity
Tiffiney R. Hartman, Erin M. Ventresca, Anthony Hopkins, Daniel Zinshteyn, Tanu Singh, Jenny A. O'Brien, Benjamin C. Neubert, Matthew G. Hartman, Heather K. Schofield, Kevin P. Stavrides, Danielle E. Talbot, Devon J. Riggs, Caroline Pritchard, Alana M. O'Reilly
Article
Oncology
Alexander Beatty, Lauren S. Fink, Tanu Singh, Alexander Strigun, Erik Peter, Christina M. Ferrer, Emmanuelle Nicolas, Kathy Q. Cai, Timothy P. Moran, Mauricio J. Reginato, Ulrike Rennefahrt, Jeffrey R. Peterson
MOLECULAR CANCER THERAPEUTICS
(2018)
Article
Cell Biology
Tanu Singh, Eric H. Lee, Tiffiney R. Hartman, Dara M. Ruiz-Whalen, Alana M. O'Reilly
DEVELOPMENTAL CELL
(2018)
Article
Genetics & Heredity
Mikael V. Garabedian, Chiaki Noguchi, Melissa A. Ziegler, Mukund M. Das, Tanu Singh, Logan J. Harper, Adam R. Leman, Lyne Khair, Bettina A. Moser, Toru M. Nakamura, Eishi Noguchi
Editorial Material
Cell Biology
Tanu Singh, Alana M. O'Reilly
Article
Genetics & Heredity
Chiaki Noguchi, Tanu Singh, Melissa A. Ziegler, Jasmine D. Peake, Lyne Khair, Ana Aza, Toru M. Nakamura, Eishi Noguchi
EPIGENETICS & CHROMATIN
(2019)
Article
Biochemistry & Molecular Biology
Katherine Kaus, Alison Biester, Ethan Chupp, Jianyi Lu, Charlie Visudharomn, Rich Olson
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Alexander Beatty, Tanu Singh, Yulia Y. Tyurina, Vladimir A. Tyurin, Svetlana Samovich, Emmanuelle Nicolas, Kristen Maslar, Yan Zhou, Kathy Q. Cai, Yinfei Tan, Sebastian Doll, Marcus Conrad, Aravind Subramanian, Valerian E. Kagan, Ulrike Rennefahrt, Jeffrey R. Peterson, Hulya Bayir
Summary: Inhibition of lipid peroxidase GPX4 promotes ferroptotic cell death. A complementary approach using conjugated linolenic fatty acids has been identified, triggering lipid peroxidation and ferroptosis via ACSL1, DGAT1/2, and neutral lipids. This may provide a potential therapeutic strategy for cancer treatment.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Alison Biester, Sebastien Dementin, Catherine L. Drennan
Summary: In this study, the gas channels in a monofunctional CODH from Desulfovibrio vulgaris were investigated by pressurizing protein crystals and solving the structure. The results revealed an unvalidated gas channel and a shared channel with another CODH, which lays the groundwork for further exploration of oxygen-tolerance factors in this CODH and study of channels in other CODHs.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Genetics & Heredity
Zachary A. Drum, Stephen M. Lanno, Sara M. Gregory, Serena J. Shimshak, Mukshud Ahamed, Will Barr, Bethlehem Bekele, Alison Biester, Colleen Castro, Lauren Connolly, Nicole DelGaudio, William Humphrey, Helen Karimi, Sophie Karolczak, Tay-Shaun Lawrence, Andrew McCracken, Noah Miller-Medzon, Leah Murphy, Cameron Park, Sojeong Park, Chloe Qiu, Kevin Serra, Gigi Snyder, Alexa Strauss, Spencer Tang, Christina Vyzas, Joseph D. Coolon
Summary: Fruit fly Drosophila sechellia is a dietary specialist endemic to the Seychelles islands, evolved to consume the fruit of Morinda citrifolia with resistance to toxic medium-chain fatty acid volatiles present in the fruit. This specialization is reflected in the differential gene expression compared to generalist fruit flies, highlighting the importance of understanding genetic mechanisms in dietary specialization and trophic interactions.
G3-GENES GENOMES GENETICS
(2022)
Article
Cell Biology
Tanu Singh, Alexander Beatty, Jeffrey R. Peterson
Summary: The study identifies NUAK2 as a regulator of GPX4 expression and ferroptotic sensitivity, suggesting its potential as a biomarker for breast cancer patients who may respond to GPX4 inhibition therapy.
CELL DEATH DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Alison Biester, Andrea N. Marcano-Delgado, Catherine L. Drennan
Summary: Ni-Fe-S-dependent carbon monoxide dehydrogenases play important roles in the microbiota of animals, facilitating the use of CO and CO2 as carbon sources and maintaining redox homeostasis. CODH/ACS is responsible for acetate production, while ACDS is involved in acetate breakdown.