Article
Cell Biology
Elizabeth S. Greene, Clay Maynard, Garrett Mullenix, Mike Bedford, Sami Dridi
Summary: In this study, using a chicken myotube culture model, it was found that hypoxia and endoplasmic reticulum stress are important causes of woody breast myopathy in broiler chickens. Hypoxia can induce cell death and increase collagen synthesis. These findings suggest potential targets for future interventions to mitigate this myopathy.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Christopher M. Lemon, Deirdre Hanley, Allison E. Batka, Michael A. Marletta
Summary: Ratiometric sensors are a type of self-referencing constructs that can function in cells and tissues, and their read-out is not affected by sensor concentration. This study developed protein-based ratiometric oxygen sensors using heme nitric oxide/oxygen-binding protein (H-NOX) and oxygen-responsive fluorescent probes. By modifying the amino acid residues on the protein surface, the oxygen sensitivity of the sensor can be adjusted.
INORGANIC CHEMISTRY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Artem Smirnov, Eleonora Candi
Summary: Skin acts as a barrier to protect the body and is directly exposed to oxygen. The role of oxygen in in vitro generation of skin graft is not clear. Teshima et al. discovered the impact of the HIF pathway on epidermal differentiation in skin models and how air-lifting of cultures affects HIF activity and keratinocyte differentiation.
Article
Agriculture, Dairy & Animal Science
Nima K. Emami, Reagan N. Cauble, Ahmed E. Dhamad, Elizabeth S. Greene, Cynthia S. Coy, Sandra G. Velleman, Sara Orlowski, Nicholas Anthony, Mike Bedford, Sami Dridi
Summary: The Woody Breast condition poses challenges to the global poultry industry with no effective treatment currently available. Research suggests that satellite cells in affected birds may undergo a shift in fate under hypoxic conditions.
Review
Cell Biology
T. van Vliet, F. Casciaro, M. Demaria
Summary: Oxygen is crucial for tissue function, disruptions in oxygen pathways lead to health issues and aging. Oxygen-modulating therapies and natural oxygen oscillations may impact lifespan and healthy aging.
AGEING RESEARCH REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Agnieszka Drzal, Anthony Delalande, Gabriela Dziurman, Chantal Pichon, Jan Swakon, Martyna Elas
Summary: The inadequate oxygenation in tumors is associated with increased invasiveness of cancer cells and reduced sensitivity to anticancer therapies. Attempts to increase tumor oxygenation have been largely ineffective. The use of phospholipid-coated oxygen microbubbles (OMB) can increase tumor oxygenation, but it also affects tumor vasculature and promotes cancer cell spread.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Developmental Biology
Yida Jiang, Li-Juan Duan, Guo-Hua Fong
Summary: This article discusses the molecular mechanisms of the oxygen-sensing pathway and the regulation of HIFα under normoxia and hypoxia conditions. Through mouse models, it investigates loss-of-function phenotypes and emphasizes the clinical relevance of HIF in angiogenesis and tissue repair.
Article
Biochemistry & Molecular Biology
Agnieszka Drzal, Gabriela Dziurman, Pawel Hola, Jakub Lechowski, Anthony Delalande, Jan Swakon, Chantal Pichon, Martyna Elas
Summary: Radiotherapy is a crucial part of cancer treatment, but challenges such as tumor hypoxia and resistance to radiation still exist. Vascular normalization, which aims to improve oxygen supply, has shown promise. This study explores the combination of metformin, an anti-diabetic drug, and oxygen microbubbles to achieve vascular normalization and enhance the effectiveness of radiotherapy. The findings demonstrate that the combination treatment has a more pronounced and sustained effect on normalizing abnormal cancer vasculature compared to individual treatments. It also increases the radiosensitivity of the tumors, although resistance to treatment and recurrence of hypoxia remain as challenges.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Peibin Zhu, Lixiong Lin, Wen Chen, Liang Liu
Summary: In this study, oxygen probes based on halogen-bridged Cu clusters were successfully synthesized in the micropores of COFs through in-situ synthesis. The resulting composite samples showed high emission quantum yield and long emissive lifetime, indicating promising oxygen-sensing performance. The change in emission color of the sample allowed for direct visual detection of oxygen concentration.
Article
Multidisciplinary Sciences
Hortensia Torres-Torrelo, Patricia Ortega-Saenz, Lin Gao, Jose Lopez-Barneo
Summary: Lactate, traditionally seen as a by-product of anaerobic metabolism, is now understood to be a crucial fuel for mitochondrial oxidative phosphorylation and a signaling molecule with medical significance. Changes in blood lactate levels can trigger adaptive responses in normal and pathological conditions. Carotid body cells are shown to sense lactate and hypoxia through separate mechanisms, but activate the same pathway to enhance compensatory respiratory reflexes.
NATURE COMMUNICATIONS
(2021)
Article
Cardiac & Cardiovascular Systems
Cooper M. Warne, Salman I. Essajee, Selina M. Tucker, C. Alberto Figueroa, Daniel A. Beard, Gregory M. Dick, Johnathan D. Tune
Summary: The study investigated the mechanisms behind coronary autoregulation. It found that hypoxemia-induced exaggeration of the metabolic error signal improved the autoregulatory response. The findings supported the presence of an autoregulatory threshold that preserves myocardial oxygen delivery during reductions in coronary perfusion pressure.
BASIC RESEARCH IN CARDIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Renbin Zhou, Tymish Y. Ohulchanskyy, Hao Xu, Roman Ziniuk, Junle Qu
Summary: Using CatCry as an oxygen-generating system can effectively alleviate tumor hypoxia and enhance PDT efficiency. The CatCry-MB drug platform significantly enhances PDT efficiency through three mechanisms, including continuous oxygen supply, shortening of diffusion distance between O-2 and MB, and avoidance of MB aggregation.
Article
Chemistry, Physical
Zheng-Liang Diao, Yong-Wen Zhang, Ji-Yang Chen, Wan-Yi Tan, Yan-Nan Qian, Lian-Gang Xiao, Yonggang Min
Summary: This study introduces a facile approach to improve the efficiency and stability of blue perovskite light-emitting diodes (PeLEDs) by using a sacrificial agent, maleic anhydride (MAH). MAH can passivate defects and absorb water, and its hydrolysis product, maleic acid (MA), can also modify the perovskite. Additionally, the use of MAH can improve the morphology of the perovskite film.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Chemistry, Physical
Yonghong Ding, Ziyue Zhao, Joerg Matysik, Wolfgang Gaertner, Aba Losi
Summary: Aromatic amino acids play a crucial role in determining the photophysical features of LOV proteins, affecting fluorescence quantum yields and lifetimes, triplet yields and lifetimes, and efficiency of singlet oxygen formation. Tryptophan and tyrosine can act as potential electron donors to the flavin excited states, while those located too close to the chromophore may reduce the yield of photoproduct formation. Adding inert phenylalanine in key positions can help in developing efficient LOV-based photosensitisers.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Physics, Applied
Raj Kumar Khan, Pratibha Ramarao
Summary: Optimizing strategies for blue phase liquid crystals directly impact device performance, with recent use of colloidal nanoparticle assemblies enhancing the BP ranges, widening the applications of BPs as photonic materials.
JOURNAL OF APPLIED PHYSICS
(2021)
Article
Biochemical Research Methods
Joel Rodriguez Troncoso, Paola Monterroso Diaz, David E. Lee, Charles M. Quick, Narasimhan Rajaram
Summary: Diffuse reflectance spectroscopy can be used to monitor tumor response to immune checkpoint inhibitors in a murine colorectal cancer model. Monotherapy with anti-PD-L1 or anti-CTLA-4 led to increased tumor vascular oxygenation, while the combined treatment group showed no significant changes in oxygenation.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Oncology
Santosh Kumar Paidi, Joel Rodriguez Troncoso, Piyush Raj, Paola Monterroso Diaz, Jesse D. Ivers, David E. Lee, Nathan L. Avaritt, Allen J. Gies, Charles M. Quick, Stephanie D. Byrum, Alan J. Tackett, Narasimhan Rajaram, Ishan Barman
Summary: This study demonstrates the potential of label-free Raman spectroscopy for analyzing biomolecular changes induced by immune checkpoint inhibitors in the tumor microenvironment, providing a new approach for predicting response to immunotherapy.
Article
Biochemistry & Molecular Biology
Isabella M. Acquistapace, Emma J. Thompson, Imke Kuhn, Mike R. Bedford, Charles A. Brearley, Andrew M. Hemmings
Summary: The role of the proton donor residue in determining the stereospecificity of the AppA phytase enzyme was investigated. Mutations in the proton donor residue strongly influenced the enzyme's activity and preference for different substrates. High-resolution crystal structures provided evidence for the importance of the proton donor residue in regulating specificity pockets A and B.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
V. R. Pirgozliev, S. C. Mansbridge, T. Kendal, E. S. Watts, S. P. Rose, C. A. Brearley, M. R. Bedford
Summary: This study examined the effect of different rapeseed meal processing methods and exogenous enzyme supplementation on key nutritional factors of broiler chickens. The results showed that the use of the MT method yielded feed with higher apparent metabolizable energy. PHY supplementation affected feed intake and feed conversion ratio in MT diets. XYL feeding reduced overall feed intake and feed conversion ratio. PHY supplementation reduced the concentration of IP6 in excreta and increased the concentration of MI. The combination of PHY and XYL feeding decreased the concentration of MI compared to PHY alone. Feeding rapeseed meal diets led to an increase in the concentration of IP6 and MI in excreta.
Article
Multidisciplinary Sciences
Gregory D. Rix, Colleen Sprigg, Hayley Whitfield, Andrew M. Hemmings, Jonathan D. Todd, Charles A. Brearley
Summary: Phylogenetic analysis, homology modelling, and biochemical methods were used to study a phytase from a Gram-negative soil bacterium. The enzyme showed stability in solution, broad pH activity profile, and specific substrate binding pockets. The transcription of the phytase gene was affected by environmental conditions.
Article
Multidisciplinary Sciences
Colleen Sprigg, Hayley Whitfield, Emily Burton, Dawn Scholey, Michael R. Bedford, Charles A. Brearley
Summary: Many studies have shown that the addition of phytase can improve the productivity of animals. However, the effect of dietary phytase on liver and kidney tissue inositol phosphates has not been thoroughly studied.
Article
Biology
April Jules, Davin Means, Joel Rodriguez Troncoso, Alric Fernandes, Sina Dadgar, Eric R. Siegel, Narasimhan Rajaram
Summary: This study used diffuse reflectance spectroscopy to assess the effects of different doses of radiation on the tumor microenvironment, finding that 1 Gy/fraction of radiation significantly increased tumor vascular oxygenation, while all doses of radiation led to a decrease in tissue scattering.
RADIATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Hayley L. Whitfield, Sining He, Yinghong Gu, Colleen Sprigg, Hui -Fen Kuo, Tzyy-Jen Chiou, Andrew M. Riley, Barry V. L. Potter, Andrew M. Hemmings, Charles A. Brearley
Summary: This study describes the differences in substrate specificity between two isoforms of ITPK enzymes (ITPK1 and ITPK4) in Arabidopsis, and provides a molecular explanation for the diverse phosphotransferase activity of ITPK4 by analyzing its ATP-coordinated structure. Furthermore, the study reveals a previously unreported N-terminal haloacid dehalogenase-like fold in ITPK4 and its homologues in other plants.
BIOCHEMICAL JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Melissa Chanderban, Christopher A. Hill, Ahmed E. Dhamad, Daniel J. Lessner
Summary: This study provides the first evidence that a methanogen can produce all three forms of nitrogenases, including simultaneously. The results reveal components of Mo-nitrogenase regulate or are needed to produce V-nitrogenase and Fe-nitrogenase in methanogens, a result not seen in bacteria. Overall, this study provides a foundation to understand the assembly, regulation, and activity of the alternative nitrogenases in methanogens.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Colleen R. Sprigg, Philip T. A. Leftwich, Emily Burton, Dawn Scholey, Michael R. Bedford, Charles A. Brearley
Summary: Inert digestibility index markers like titanium dioxide are commonly used in poultry feeding trials. However, the use of titanium dioxide in cell biology for enrichment of inositol phosphates suggests that its use as an inert marker may not be as inert as assumed. Our experiment showed no significant difference in the effect of phytase inclusion in the presence or absence of titanium dioxide, indicating that its inclusion in feed does not interfere with the recovery of inositol phosphates from digesta samples.
Meeting Abstract
Oncology
Santosh K. Paidi, Joel R. Troncoso, Narasimhan Rajaram, Ishan Barman
Meeting Abstract
Oncology
Santosh K. Paidi, Joel R. Troncoso, Narasimhan Rajaram, Ishan Barman
Article
Medicine, Research & Experimental
Santosh Kumar Paidi, Joel Rodriguez Troncoso, Mason G. Harper, Zhenhui Liu, Khue G. Nguyen, Sruthi Ravindranathan, Lisa Rebello, David E. Lee, Jesse D. Ivers, David A. Zaharoff, Narasimhan Rajaram, Ishan Barman
Summary: The use of Raman spectroscopy and machine learning allows for the identification of metastatic phenotype in tumors. Spectral analysis of tumors can predict their metastatic potential and evaluate the risk of metastasis in primary tumor biopsies of clinical patients.
Article
Biochemistry & Molecular Biology
Raquel Faba-Rodriguez, Yinghong Gu, Melissa Salmon, Giuseppe Dionisio, Henrik Brinch-Pedersen, Charles A. Brearley, Andrew M. Hemmings
Summary: This study presents the X-ray crystal structure of the b2 isoform of wheat PAPhy induced during germination, revealing a model for phytate recognition and its impact on the activity of cereal PAPhys, laying the groundwork for rational engineering of phytase activity in plants.
PLANT COMMUNICATIONS
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Nagma Vohra, Tanny Chavez, Joel R. Troncoso, Narasimhan Rajaram, Jingxian Wu, Patricia N. Coan, Todd A. Jackson, Keith Bailey, Magda El-Shenawee
Summary: This study quantitatively evaluated the potential of THz imaging in differentiating cancerous from non-cancerous tissues in mammary tumors developed in Sprague Dawley rats injected with ENU. Results showed that high reflection THz signals were able to distinguish cancerous tissues, and the implemented EM classification algorithm achieved decent tumor classification in both fresh and fixed tissues.
JOURNAL OF MEDICAL IMAGING
(2021)