Article
Chemistry, Multidisciplinary
Sonia Serna, Raik Artschwager, Damian Perez-Martinez, Rosa Lopez, Niels-Christian Reichardt
Summary: The isolation of glycoproteins has become a convenient source of N-glycans for various applications. This study focuses on the development of a UV active linker for attaching to anomeric glycosylamines and compares its performance on glycan arrays with other linkers. The results show that the urea type linker exhibits superior performance in lectin binding and emphasizes the influence of linker choice on lectin recognition.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Immunology
Hyeongjwa Choi, Seung-Woo Yang, Jin-Soo Joo, Min Park, Yihua Jin, Ji-Woon Kim, Seon-Yeong Lee, Sung-Vin Lee, Tae-Jin Yun, Mi-La Cho, Han-Sung Hwang, Young-Sun Kang
Summary: Although the exact action mechanisms of IVIg are not fully understood, the interaction between DC-SIGN and sIVIg in HBCs promotes IL-10 secretion and enhances immune tolerance. This suggests the therapeutic application of sIVIg to prevent preeclampsia, a major complication in pregnancy.
CLINICAL IMMUNOLOGY
(2023)
Review
Cell Biology
Franziska Krautter, Asif J. Iqbal
Summary: The recruitment of leukocytes is a complex process involving interactions between proteins expressed by the endothelium and circulating leukocytes. Recent research has expanded our understanding of these interactions, identifying novel lectins and regulatory pathways. Targeting glycans and glycan-binding proteins could offer new therapeutic strategies for modulating leukocyte recruitment and transmigration during inflammation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Giulio Fittolani, Elena Shanina, Monica Guberman, Peter H. Seeberger, Christoph Rademacher, Martina Delbianco
Summary: This study presents a rapid approach that combines automated glycan assembly with high-throughput NMR methods for studying protein-glycan interactions. Through screening synthetic Lewis type 2 antigens, preferences for fucosylated glycans were confirmed, and previously unknown weak interactions between glycans and lectins were discovered.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Asako Shimoda, Risako Miura, Hiroaki Tateno, Naohiro Seo, Hiroshi Shiku, Shin-ichi Sawada, Yoshihiro Sasaki, Kazunari Akiyoshi
Summary: The study found both similarities and differences in glycan patterns on EVs obtained under different experimental conditions. EV size and isolation method impact lectin-binding patterns. Additionally, cellular uptake behaviors of EVs are influenced by EV glycan profiles and acceptor cells.
Article
Biology
Tongcui Ma, Matthew McGregor, Leila Giron, Guorui Xie, Ashley F. George, Mohamed Abdel-Mohsen, Nadia R. Roan
Summary: This study develops a method called CyTOF-Lec that allows for simultaneous characterization of protein and glycan features of human immune cells at the single-cell level. It finds that HIV infection not only remodels cellular proteins but also glycans, and that glycan expression can differentiate memory CD4+ T cells with varying susceptibility to HIV infection.
Article
Chemistry, Organic
Zoeisha S. Chinoy, Kelley W. Moremen, Frederic Friscourt
Summary: This study reports a new chemical tool, sydnone-aglycon, that can be used for the synthesis of O-glycans and the high-throughput screening of O-glycan-lectin interactions.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Irene Herrera-Gonzalez, Michel Thepaut, Elena M. Sanchez-Fernandez, Antonio di Maio, Corinne Vives, Javier Rojo, Jose M. Garcia Fernandez, Franck Fieschi, Pedro M. Nieto, Carmen Ortiz Mellet
Summary: Selective targeting of DC-SIGN and langerin may lead to the development of anti-infective agents. This study demonstrates that multivalent sp(2)-iminosugar-containing mannobioside analogs can achieve total DC-SIGN selectivity by leveraging the canonical binding mode towards high-mannose oligosaccharide ligands, acting as real biomimics.
CHEMICAL COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Ruben Warkentin, David H. Kwan
Summary: This review provides information on available methods for engineering glycan-binding proteins (GBPs) and highlights the importance of glycans in physiological functions and their applications. Recent advances in glycobiology have largely overcome challenges in developing GBPs.
Article
Cardiac & Cardiovascular Systems
Koki Mise, Mariko Imamura, Satoshi Yamaguchi, Mayu Watanabe, Chigusa Higuchi, Akihiro Katayama, Satoshi Miyamoto, Haruhito A. Uchida, Atsuko Nakatsuka, Jun Eguchi, Kazuyuki Hida, Tatsuaki Nakato, Atsuhito Tone, Sanae Teshigawara, Takashi Matsuoka, Shinji Kamei, Kazutoshi Murakami, Ikki Shimizu, Katsuhiro Miyashita, Shinichiro Ando, Tomokazu Nunoue, Michihiro Yoshida, Masao Yamada, Kenichi Shikata, Jun Wada
Summary: The study found that urinary glycan signals binding to two specific lectins were significantly associated with CVE, particularly the high-mannose type of N-glycans. Adding the glycan index for UDA to the model improved outcome prediction significantly.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Razie Amraei, Wenqing Yin, Marc A. Napoleon, Ellen L. Suder, Jacob Berrigan, Qing Zhao, Judith Olejnik, Kevin Brown Chandler, Chaoshuang Xia, Jared Feldman, Blake M. Hauser, Timothy M. Caradonna, Aaron G. Schmidt, Suryaram Gummuluru, Elke Muehlberger, Vipul Chitalia, Catherine E. Costello, Nader Rahimi
Summary: The study identified CD209L and CD209 as receptors for SARS-CoV-2 entry into human cells. CD209L is prominently expressed in lung and kidney tissues and interacts with ACE2, potentially playing a role in SARS-CoV-2 infection. This finding may have implications for antiviral drug development in tissues where ACE2 expression is low or absent.
ACS CENTRAL SCIENCE
(2021)
Article
Oncology
Michal Hires, Eduard Jane, Katarina Kalavska, Michal Chovanec, Michal Mego, Peter Kasak, Tomas Bertok, Jan Tkac
Summary: This study investigates changes in glycosylation associated with cisplatin resistance in testicular cancer cell lines. The results suggest that a specific lectin called Dolichos biflorus agglutinin (DBA) can selectively discriminate between sensitive and resistant cell lines, and other lectins can provide useful information about the strength of cisplatin resistance.
Article
Biochemistry & Molecular Biology
Ji Young Hyun, Sanggil Kim, Chang-Hee Lee, Hyun Soo Lee, Injae Shin
Summary: This study presents a general method for efficient preparation of homogeneous glycoproteins using genetic code expansion and chemoselective ligation techniques. The glycoproteins produced through this method can recognize mammalian cell-surface lectins and enter cells, showing potential for biomedical applications.
ACS CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Irene Herrera-Gonzalez, Manuel Gonzalez-Cuesta, Michel Thepaut, Eugenie Laigre, David Goyard, Javier Rojo, Jose M. Garcia Fernandez, Franck Fieschi, Olivier Renaudet, Pedro M. Nieto, Carmen Ortiz Mellet
Summary: The carbohydrate chemical mimicry of sp2-iminosugars enables the synthesis of high-mannose-type oligosaccharide analogs whose solution and lectin bound conformations match those of the natural partners. The ability to finely modulate the binding affinity and selectivity towards specific lectins by incorporating the OMJ sp2-iminosugar caps offers potential applications in various fields.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Meina Gao, Hui Li, Chenghao Ye, Kaixian Chen, Hualiang Jiang, Kunqian Yu
Summary: The study identified the crucial mechanism of DC-SIGN binding to the SARS-CoV-2 Spike protein, providing a novel approach for exploring COVID-19 treatments, and predicted potential glycan epitopes. Additionally, molecular dynamics simulations revealed specific binding pockets for oligosaccharides.