Review
Biochemistry & Molecular Biology
Markus Axmann, Birgit Plochberger, Mario Mikula, Florian Weber, Witta Monika Strobl, Herbert Stangl
Summary: Cholesterol transfer between lipoprotein particles and the plasma membrane is crucial for lipid supply in the human body. Different density lipoprotein particles deliver cholesterol to cells through redundant pathways, which can compensate for defects in one pathway but may lead to systemic diseases later on. Intracellular membrane-membrane contact sites and the plasma membrane itself play roles in cholesterol transfer.
Article
Biochemistry & Molecular Biology
Ryoka Uchiyama, Eriko Toyoda, Miki Maehara, Shiho Wasai, Haruka Omura, Masahiko Watanabe, Masato Sato
Summary: PRP purified using commercial kits can affect macrophage polarization, with APS having a higher concentration of M1 and M2 macrophage related factors. When PRP supernatants were added to monocyte-derived macrophages and M1 polarized macrophages, it was observed that PRPs suppressed M1 macrophage polarization and promoted M2 macrophage polarization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Arrigo F. G. Cicero, Federica Fogacci, Antonio Di Micoli, Maddalena Veronesi, Elisa Grandi, Claudio Borghi
Summary: This study evaluated the effect of dietary supplementation with highly standardized polyphenols derived from Coratina Olive on the plasma cholesterol levels of hypercholesterolemic individuals. Results showed significant improvement in several blood markers with this supplementation, indicating its potential in the prevention and treatment of metabolic syndrome.
APPLIED SCIENCES-BASEL
(2022)
Article
Multidisciplinary Sciences
Kenta Shigetomi, Yumiko Ono, Kenji Matsuzawa, Junichi Ikenouchi
Summary: It has been found that cholesterol accumulation is essential for the formation of tight junctions, which are crucial for the epithelial barrier. Despite the absence of tight junctions, cholesterol still accumulates normally in the vicinity of the apical junctions. Moreover, a claudin mutant that cannot bind to Zonula Occludens (ZO) proteins can still form tight junction strands. ZO proteins are not only scaffolds for claudins, but also promote the formation of cholesterol-rich membrane domains at apical junctions through their effect on the junctional actomyosin cytoskeleton.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Eric T. Hall, Miriam E. Dillard, Daniel P. Stewart, Yan Zhang, Ben Wagner, Rachel M. Levine, Shondra M. Pruett-Miller, April Sykes, Jamshid Temirov, Richard E. Cheney, Motomi Mori, Camenzind G. Robinson, Stacey K. Ogden
Summary: Morphogens act in concentration-dependent ways to guide cell fate during tissue patterning, with cytoneme transport model suggesting the extension of specialized filopodia between sending and responding cells to ensure proper signaling thresholds. The actin motor Myosin 10 facilitates vesicular transport of Sonic Hedgehog (SHH) morphogen in mouse cell cytonemes, and cytoneme-mediated deposition of SHH onto receiving cells induces a rapid, receptor-dependent signal response. The activity is dependent on a novel Dispatched (DISP)-BOC/CDON co-receptor complex in ligand-producing cells to promote cytoneme occurrence and facilitate signal activation.
Article
Biochemistry & Molecular Biology
Bilge Ercan, Tomoki Naito, Dylan Hong Zheng Koh, Dennis Dharmawan, Yasunori Saheki
Summary: The GRAM domain of GRAMD1b plays a crucial role in detecting cholesterol and anionic lipids, with mutations potentially leading to intellectual disabilities in humans. Cell utilize the GRAM domain to regulate the rate of cholesterol transport and membrane tethering.
Article
Biochemistry & Molecular Biology
Natalia Ruiz-Lafuente, Alfredo Minguela, Manuel Muro, Antonio Parrado
Summary: The inducible model from HeLa cells was used to study the interactions of Cdc42 and Rac1 with DOCK10 and DOCK9 in regulating plasma membrane protrusions. The study showed that Cdc42 promotes filopodia regardless of its conformation, while Rac1 induces blebs in non-CA conformations, especially WT-Rac1, in the inducible HeLa clones.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Li Zhu, Hongjiao Li, Jiyu Li, Yuan Zhong, Shuai Wu, Meng Yan, Sheng Ni, Kun Zhang, Guixue Wang, Kai Qu, Deqin Yang, Xian Qin, Wei Wu
Summary: A disorder of cholesterol homeostasis initiates atherosclerosis progression. Macrophage membrane-camouflaged methotrexate nanoparticles (MM@MTX NPs) showed improved therapeutic efficacy by promoting cholesterol efflux and inhibiting foam cell formation. The NPs significantly reduced plaque area, aortic plaque, and cholesterol crystal deposition in ApoE-/- mice, and exhibited good biocompatibility.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yueqi Niu, Si Jia Chen, Jeffery B. Klauda
Summary: Macrophage membranes in the activated state are more tightly packed, exhibit increased chain order across lipid species, and form specific lipid clusters. These findings provide physiologically accurate models for future computational studies of macrophage membranes and their proteins.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2024)
Article
Biochemical Research Methods
Alexander J. Bryer, Tyler Reddy, Edward Lyman, Juan R. Perilla
Summary: Enveloped viruses are protected by a lipid membrane, and the complex lipid composition of the envelope is closely related to its structural, mechanical, and dynamical properties.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Review
Microbiology
Kai Zhang
Summary: Leishmania parasites rely on different sources of lipids during their life cycle stages in the sand fly vector and vertebrate host, reflecting their growth rate and metabolic activity. Future studies may reveal how Leishmania uptake and remodel host lipids at cellular and molecular levels, and blocking this lipid transfer could be a novel strategy to control Leishmania growth.
CURRENT OPINION IN MICROBIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Huirong Jiang, Zongguang Tai, Huijun Pan, Zhen Cui, Rongrong Chai, Congcong Zhu, Jing Tian, Leilei Bao, Quangang Zhu, Zhongjian Chen
Summary: A study found that macrophage membrane-derived biomimetic nanoparticles have potential in the treatment of cytokine release syndrome (CRS). By fusing macrophage membrane onto the surface of nanoparticles, the biomimetic nanoparticles can neutralize CRS-related cytokines. Among the three types of macrophage membranes compared, M0 macrophage membrane-derived biomimetic nanoparticles showed the best efficacy in reducing organ damage in a mouse model of CRS.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)
Article
Microbiology
Liang Zhang, Yanyan Yi, Tao Wang, Mengzhao Song, Kangkang Guo, Yanming Zhang
Summary: Classical swine fever virus (CSFV) utilizes cellular lipids and manipulates host lipid metabolism to ensure its replication. In this study, we screened the anti-CSFV activity of 12 compounds targeting lipid metabolism pathways. 25-hydroxycholesterol (25HC) exhibited potent anti-CSFV activity by inhibiting virus entry into cells.
VETERINARY MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Orsolya Geda, Tamas Tabi, Peter P. Lakatos, Eva Szoko
Summary: This study investigated the effects of DIMEB, HPBCD, RAMEA, and HPACD on ganglioside and cholesterol levels in rat brain synaptosomes. HPBCD showed selective cholesterol depletion capability, while RAMEA and HPACD selectively depleted gangliosides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Chemical
Fangfang Wei, Feng Qi, Yangyang Li, Weiyu Dou, Tangye Zeng, Jing Wang, Zhikan Yao, Lin Zhang, Zhe Tang
Summary: In this study, PAN/PEI nanofiber membrane (NFM) with amino-rich and smooth surface structure was prepared through a more facile co-blended electrospun method. PAN/PEI NFMs exhibited a high adsorption capacity of 142.79 mg g-1 for free bilirubin at clinical bilirubin concentration (100 mg L-1) in patients with liver failure. The development of this facile method promotes the feasibility of nanofibrous membranes for the treatment of hyperbilirubinemia with significant implications for improving therapeutic efficacy.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Ka-Chung Tong, Chun-Nam Lok, Pui-Ki Wan, Di Hu, Yi Man Eva Fung, Xiao-Yong Chang, Song Huang, Haibo Jiang, Chi-Ming Che
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Review
Neurosciences
David Hartnell, Wendy Andrews, Nicole Smith, Haibo Jiang, Erin McAllum, Ramesh Rajan, Frederick Colbourne, Melinda Fitzgerald, Virginie Lam, Ryusuke Takechi, M. Jake Pushie, Michael E. Kelly, Mark J. Hackett
FRONTIERS IN NEUROSCIENCE
(2020)
Editorial Material
Biochemistry & Molecular Biology
Haibo Jiang, Cuiwen He, Loren G. Fong, Stephen G. Young
JOURNAL OF LIPID RESEARCH
(2020)
Article
Multidisciplinary Sciences
Cuiwen He, Haibo Jiang, Wenxin Song, Howard Riezman, Peter Tontonoz, Thomas A. Weston, Paul Guagliardo, Paul H. Kim, Rachel Jung, Patrick Heizer, Loren G. Fong, Stephen G. Young
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Engineering, Environmental
Luqing Zhang, Haibo Jiang, Wen-Xiong Wang
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Alessandra Ferrari, Cuiwen He, John Paul Kennelly, Jaspreet Sandhu, Xu Xiao, Xun Chi, Haibo Jiang, Stephen G. Young, Peter Tontonoz
MOLECULAR AND CELLULAR BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Cuiwen He, Wenxin Song, Thomas A. Weston, Caitlyn Tran, Ira Kurtz, Jonathan E. Zuckerman, Paul Guagliardo, Jeffrey H. Miner, Sergey Ivanov, Jeremy Bougoure, Billy G. Hudson, Selene Colon, Paul A. Voziyan, Gautam Bhave, Loren G. Fong, Stephen G. Young, Haibo Jiang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Engineering, Environmental
Zishuang Shao, Paul Guagliardo, Haibo Jiang, Wen-Xiong Wang
Summary: This study utilized high-resolution nanoscale secondary ion mass spectrometry and scanning electron microscopy to visualize the subcellular localization and potential toxicity effects of AgNPs in oyster gill filaments. The findings demonstrated an endocytosis-based entry of AgNPs into cells and identified AgNP-induced autophagy in oyster gill cells. Additionally, the study revealed the transport and sequestration roles of different hemocytes in AgNP distribution.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cuiwen He, Michael T. Migawa, Kai Chen, Thomas A. Weston, Michael Tanowitz, Wenxin Song, Paul Guagliardo, K. Swaminathan Iyer, C. Frank Bennett, Loren G. Fong, Punit P. Seth, Stephen G. Young, Haibo Jiang
Summary: Nucleic acid therapeutics have shown promise in modifying gene expression, but there is limited understanding of their cellular uptake and tissue distribution. Utilizing NanoSIMS imaging allows for high sensitivity and spatial resolution in defining the distribution of antisense oligonucleotides, potentially improving the efficacy and delivery of nucleic acid therapeutics for treating human diseases.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Antony Fearns, Daniel J. Greenwood, Angela Rodgers, Haibo Jiang, Maximiliano G. Gutierrez
Article
Materials Science, Multidisciplinary
Mei Cui, Wei Guo, Houqiang Xu, Jie'an Jiang, Li Chen, Somak Mitra, Iman S. Roqan, Haibo Jiang, Xiaohang Li, Jichun Ye
Summary: The research demonstrates that introducing opposite polarity domains in AlGaN-based multiple quantum wells can improve the optical properties of UV emitters, despite the inferior crystalline quality in N-polar domains. By combining space-resolved luminescence peak position and Raman spectroscopy analysis, it is confirmed that carrier localization effect exists in the MQWs.
PHYSICA STATUS SOLIDI-RAPID RESEARCH LETTERS
(2021)
Article
Engineering, Environmental
Nanyan Weng, Paul Guagliardo, Haibo Jiang, Wen-Xiong Wang
Summary: This study investigated the distribution and accumulation of manganese in oyster oocytes and sperm cells, revealing significant differences in subcellular localization and accumulation capacity between the two cell types. Manganese was found to mainly accumulate in cortical granules of oocytes, while it was primarily distributed in the nucleus of sperm cells, indicating sex differentiation in susceptibility to manganese stress. This highlights the importance of considering gender differences in future biomonitoring and ecotoxicological studies of manganese in marine bivalves.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Pierre Santucci, Daniel J. Greenwood, Antony Fearns, Kai Chen, Haibo Jiang, Maximiliano G. Gutierrez
Summary: It is crucial for tuberculosis chemotherapy to kill the intracellular population of the pathogen, and PZA accumulates and is most effective in acidified phagosomes. Combination with bedaquiline enhances PZA accumulation by a host-dependent process.
NATURE COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Kai Chen, Shijie Liao, Yicheng Li, Haibo Jiang, Yun Liu, Chao Wang, Vincent Kuek, Jacob Kenny, Boxiang Li, Qian Huang, Jianxin Hong, Yan Huang, Shek Man Chim, Jennifer Tickner, Nathan J. Pavlos, Jinmin Zhao, Qian Liu, An Qin, Jiake Xu
Summary: EGFL6 serves as a crucial regulator in coupling osteogenesis to angiogenesis during bone repair. Overexpression of EGFL6 enhances osteogenic capacity, while deficiency leads to compromised bone repair.
Article
Chemistry, Analytical
Anna Maria Ranieri, Matteo Vezzelli, Kathryn G. Leslie, Song Huang, Stefano Stagni, Denis Jacquemin, Haibo Jiang, Alysia Hubbard, Luca Rigamonti, Elizabeth L. J. Watkin, Mark I. Ogden, Elizabeth J. New, Massimiliano Massi
Summary: New organometallic molecular probes have been developed for bacterial imaging, with the potential to support multiple imaging modalities.