Article
Cardiac & Cardiovascular Systems
Chang Liu, Ruihua Li, Yang Liu, Zhenguo Li, Yujiao Sun, Peiyuan Yin, Rihong Huang
Summary: This study identified significant metabolic differences in patients with CHD-, VHD-, and DCM-induced heart failure. Certain metabolites were found to be related to the occurrence and differentiation of HF subtypes. Circulating amino acids and long-chain acylcarnitine levels were closely associated with heart failure progression. Monitoring these metabolic alterations may aid in early differentiation and offer new diagnostic or therapeutic targets.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cell Biology
Juntuo Zhou, Xi Chen, Wei Chen, Lijun Zhong, Ming Cui
Summary: Using plasma metabolomic and lipidomic analysis, this study identified potential biomarkers correlated with different stages of heart failure. Several dysregulated molecules were found, and marker panels were proposed for the diagnosis of heart failure stages. This research provides valuable information for further studies and biomarker development in heart failure.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Donghui Lao, Rong Liu, Jianying Liang
Summary: By applying metabolomics, potential biomarkers for AIDS patients have been identified and verified, suggesting their potential role in the diagnosis of HIV/AIDS.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Obstetrics & Gynecology
See Ling Loy, Jieliang Zhou, Liang Cui, Tse Yeun Tan, Tat Xin Ee, Bernard Su Min Chern, Jerry Kok Yen Chan, Yie Hou Lee
Summary: The study identified potential biomarkers for peritoneal endometriosis in peritoneal fluid and serum using metabolomics analysis. Phenylalanyl-isoleucine showed promising discriminatory performance in distinguishing peritoneal endometriosis from controls, particularly in the proliferative phase. Further research is needed to validate these findings and assess the clinical utility of phenylalanyl-isoleucine as a diagnostic biomarker for peritoneal endometriosis.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Review
Oncology
Andrew Gold, Fouad Choueiry, Ning Jin, Xiaokui Mo, Jiangjiang Zhu
Summary: Colorectal Cancer (CRC) is a leading cause of cancer-related death in the United States and current diagnosis techniques lack sufficient sensitivity. Metabolomics is a promising technique for identifying CRC biomarkers with high sensitivity and specificity, but it is rarely used in clinical settings. This study conducted a systematic review of recent literature on the detection of CRC biomarkers through metabolomics, identifying dysregulated metabolites across different biospecimens. The study also highlighted the interplay between the gut microbiome and perturbed metabolites in CRC. The findings demonstrate the value of metabolomics as a promising diagnostic technique for CRC.
Article
Food Science & Technology
Run Zhang, Man Yang, Xinhua Hou, Renda Hou, Ligang Wang, Lijun Shi, Fuping Zhao, Xin Liu, Qingshi Meng, Lixian Wang, Longchao Zhang
Summary: This study investigated the characterization and difference of lipids and metabolites between JWX and LW pork using metabolomic and lipidomic profiling. The results showed that JWX pork had higher intramuscular fat content and meat redness compared to LW pork. The composition of metabolites and lipids also showed significant differences between the two types of pork.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Agriculture, Multidisciplinary
Guanshi Zhang, Dawid Tobolski, Grzegorz Zwierzchowski, Rupasri Mandal, David S. Wishart, Burim N. Ametaj
Summary: Metabolomics analysis can be used to identify potential biomarkers for subclinical mastitis in dairy cows. The study also found that specific metabolic pathways are affected by the disease.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Integrative & Complementary Medicine
Hong Zong-Chao, Cai Quan, Wu He-Zhen, Yang Yan-Fang, Fan Heng, Duan Xue-Yun
Summary: Ulcerative colitis is a chronic disease that is difficult to cure, new biomarkers can aid in understanding and early diagnosis. Compound Sophorae Decoction is effective in treating UC by regulating multiple metabolic pathways.
CHINESE JOURNAL OF NATURAL MEDICINES
(2021)
Article
Biochemical Research Methods
Kaiyang Wang, Lan Luo, Xiaoli Xu, Xingyu Chen, Qiong He, Zhongjie Zou, Shumei Wang, Shengwang Liang
Summary: This study validated the effectiveness of hawthorn in improving gastrointestinal motility disorder in a rat model, with ethyl acetate extract (T3) showing the best therapeutic effect in promoting gastrointestinal motility. Other parts showed no obvious therapeutic effect, suggesting that the effective components of hawthorn may be compounds of medium polarity.
BIOMEDICAL CHROMATOGRAPHY
(2021)
Article
Nutrition & Dietetics
Stefania Noerman, Jyrki K. Virtanen, Marko Lehtonen, Carl Brunius, Kati Hanhineva
Summary: The study aimed to identify fasting serum metabolites associated with whole grain intake in a free-living population. The findings suggested strong associations between whole grain intake and several metabolites, which remained significant even after adjusting for potential confounders. These metabolites have the potential to serve as robust biomarkers for whole grain consumption.
EUROPEAN JOURNAL OF NUTRITION
(2023)
Article
Microbiology
Maryam Mapar, Thomas Rydzak, Ryan A. Groves, Ian A. Lewis
Summary: Microbes have diverse metabolic capabilities and differences in these phenotypes are critical for differentiating strains, species, and broader taxa of microorganisms. Recent advances in liquid chromatography-mass spectrometry (LC-MS) allow researchers to track the complex combinations of molecules that are taken up by each cell type and to quantify the rates that individual metabolites enter or exit the cells. This metabolomics-based approach allows complex metabolic phenotypes to be captured in a single assay, enables computational models of microbial metabolism to be constructed, and can serve as a diagnostic approach for clinical microbiology.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Samuel Bernardo-Bermejo, Jingchuan Xue, Linh Hoang, Elizabeth Billings, Bill Webb, M. Willy Honders, Sanne Venneker, Bram Heijs, Maria Castro-Puyana, Maria Luisa Marina, Erik B. van den Akker, Marieke Griffioen, Gary Siuzdak, Martin Giera, Elena Sanchez-Lopez
Summary: Analytical techniques with high sensitivity and selectivity are crucial in clinical sample analysis. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is the standard method, but it is expensive. This study demonstrates that similar results can be achieved using a simpler single mass spectrometry detector combined with enhanced in-source fragmentation/annotation and correlated ion monitoring. The protocol provides step-by-step instructions for optimizing the conditions for this technique, making it broadly applicable and cost-effective for quantifying small molecules.
Article
Medicine, General & Internal
Huanhuan Liu, Minmin Xu, Qiongzi He, Peng Wei, Mengying Ke, Shijia Liu
Summary: In this study, specific biomarkers for Crohn's disease (CD) were identified through serum metabolomics analysis. Deoxycholic acid and palmitic amide were found to be potential predictive biomarkers for CD diagnosis. These biomarkers can effectively discriminate CD patients from healthy controls and suggest a potential association between CD metabolic disturbance and bile acid biosynthesis, fatty acids, and energy metabolism.
FRONTIERS IN MEDICINE
(2022)
Article
Biochemical Research Methods
Kirsten E. E. Overdahl, Justin B. B. Collier, Anton M. M. Jetten, Alan K. K. Jarmusch
Summary: Feature finding is a common method in processing untargeted mass spectrometry data to obtain a list of chemicals present in a sample. This study introduces signal response evaluation as a method to assess the individual features observed in untargeted MS data.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Shai Dagan, Dana Marder, Nitzan Tzanani, Eyal Drug, Hagit Prihed, Lilach Yishai-Aviram
Summary: This study investigated the impact of matrix complexity on nontargeted detection by LC-MS/MS (Orbitrap) analysis. The results showed that detection limits for nontargets were higher in complex matrices and required higher concentrations. A quantitative method was used to assess and compare the complexity of matrices and predict the typical detection limits of unknowns.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Julia J. Griese, Ramona Kositzki, Peer Schrapers, Rui M. M. Branca, Anders Nordstrom, Janne Lehtio, Michael Haumann, Martin Hogbom
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Oncology
Claudia Staubert, Rosanna Krakowsky, Hasanuzzaman Bhuiyan, Barbara Witek, Anna Lindahl, Oliver Broom, Anders Nordstrom
Article
Endocrinology & Metabolism
Anna Lindahl, Rainer Heuchel, Jenny Forshed, Janne Lehtio, Matthias Lohr, Anders Nordstrom
Article
Biochemistry & Molecular Biology
Riccardo Diamanti, Vivek Srinivas, Annika Johansson, Anders Nordstrom, Julia J. Griese, Hugo Lebrette, Martin Hogbom
Summary: In this study, we characterized the ligands copurified with R2lox using mass spectrometry-based metabolomics. We also determined the crystal structures of two new homologs of R2lox, providing new insights into putative mechanisms regulating the function of the enzyme.
Article
Chemistry, Analytical
Anna Lindahl, Siv Saaf, Janne Lehtio, Anders Nordstrom
ANALYTICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Soojung Hahn, Gyuri Kim, Sang-Man Jin, Jae Hyeon Kim
Summary: This study utilized three-dimensional intestinal organoids to investigate the effects of metformin on inflammatory bowel disease (IBD) and found that metformin can enhance intestinal barrier function and reduce levels of inflammatory cytokines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
V. V. Sudarev, M. S. Gette, S. V. Bazhenov, O. M. Tilinova, E. V. Zinovev, I. V. Manukhov, A. I. Kuklin, Yu. L. Ryzhykau, A. V. Vlasov
Summary: This study investigated the self-assembly processes of ferritin-based protein complexes and obtained structurally characterized oligomeric states. These results provide new potential and opportunities for the application of ferritin in various fields.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Yalda Sabaghi, Farnaz Pourfarzad, Leila Zolghadr, Azita Bahrami, Tahereh Shojazadeh, Alireza Farasat, Nematollah Gheibi
Summary: p-Coumaric acid (p-CA) is a plant compound with anti-cancer activities. This study designed a nano-liposomal carrier containing p-CA to enhance its effectiveness against melanoma cells. The findings showed that the liposomal form of p-CA had a greater impact on the cells. Kinetic modeling indicated that the best fitting model was zero-order.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
M. D. Nazmul Hasan, Md Mahfuzur Rahman, Al Asmaul Husna, Nobuhiro Nozaki, Osamu Yamato, Naoki Miura
Summary: This study investigated the expression of ncRNAs other than miRNAs in different histologic subtypes of canine mammary gland tumors (MGT). Three aberrantly expressed ncRNAs were identified as potential biomarkers for differentiating MGT subtypes. YRNA and tRFs expression levels were found to be decreased in metastatic compared to primary MGT cell lines.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Seine A. Shintani
Summary: In this study, the researchers used signal analysis to study the instantaneous amplitude and phase of sarcomeric oscillations in skeletal muscle. They identified two types of oscillations, sarcomeric oscillations and sarcosynced oscillations, and visualized their behavior during propagating waves. The researchers discovered the presence of sarcomeric defect holes and sarcomeric collision holes, which are important indicators for understanding the oscillation properties of sarcomeres. This finding has important implications for improving our understanding of muscle function and its regulatory mechanisms.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Shuanglin Zhang, Yuzhong Jia, Guolan Ma, Yanyan Yang, Zhenzhen Cao, Antao Luo, Zefu Zhang, Shihan Li, Jie Wen, Hanfeng Liu, Jihua Ma
Summary: Bupleurum is an antiarrhythmic agent that may exert its effects by inhibiting L-type calcium channels.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Tomotaka Ohkubo, Yasuhiko Matsumoto, Hiroaki Sasaki, Kaoru Kinoshita, Yuki Ogasawara, Takashi Sugita
Summary: This study found that Citrobacter koseri inhibits the growth of Staphylococcus epidermidis, disrupting the balance between S. epidermidis and Staphylococcus aureus, and exacerbating inflammation in atopic dermatitis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Toshifumi Asano, Philipp Sasse, Takao Nakata
Summary: A Cre recombination-based fluorescent reporter system was developed to monitor cell-cell fusion. The system successfully detected the formation of multinuclear myotubes and placental syncytiotrophoblast. This tool could facilitate the study of cell-to-cell fusion.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Ke Shi, Yunlong Shan, Xiao Sun, Kuida Chen, Qiong Luo, Qiang Xu
Summary: This study found that low expression of TP53INP2 is associated with poor survival in colorectal cancer (CRC) patients. As the malignancy of CRC progresses, TP53INP2 expression gradually decreases. Knockdown of TP53INP2 promotes CRC cell proliferation and tumor growth. Mechanistically, TP53INP2 deficiency decreases phosphorylation of beta-catenin, leading to increased accumulation and enhanced nuclear translocation and transcriptional activity. Additionally, TP53INP2 sequesters TIM50, inhibiting its activation of beta-catenin. In conclusion, downregulation of TP53INP2 promotes CRC progression by activating beta-catenin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Martina Rossi, Fabio Tomaselli, Alejandro Hochkoeppler
Summary: Oligomeric enzymes are known for their higher catalytic rates compared to monomeric enzymes, but the extent of additivity in their activity is still not well understood. This study used tetrameric rabbit lactate dehydrogenase as a model to examine the kinetics of its catalytic action. Surprisingly, when the concentration of the limiting reactant exceeded that of a single subunit, there was a significant slowdown in the enzyme's conformational rearrangements.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Amin Sahraei, Mohammad Javad Shamsoddini, Fakhrossadat Mohammadi, Leila Hassani
Summary: This study explored the inhibitory effects of gallium curcumin, indium curcumin, and vanadyl curcumin on the amyloid fibrillation of hen egg white lysozyme, as well as the binding interactions of these metal complexes with the enzyme. The results showed that indium curcumin and vanadyl curcumin exhibited higher binding affinities and stronger inhibitory effects on amyloid fibrillation compared to gallium curcumin.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Takahiro Sasaki, Yoshiki Kuse, Shinsuke Nakamura, Masamitsu Shimazawa
Summary: PGRN deficiency plays a significant role in cardiac remodeling and arrhythmias post-myocardial infarction (MI), potentially by promoting metabolic abnormalities in macrophages.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Hongwei Zhao, Yiqiang Li, Yibo Zhang, Chi Zhang
Summary: Electrical brain stimulation technology is commonly used to treat brain neurological disorders, but it can cause side effects. This study investigated the impact of electric fields on nerve fibers and revealed the possible origin of side effects. The findings provide guidance for selecting electrical parameters in clinical stimulation therapy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Julia S. Scott, Lake-Ee Quek, Andrew J. Hoy, Johannes V. Swinnen, Zeyad D. Nassar, Lisa M. Butler
Summary: The fatty acid elongation enzyme ELOVL5 plays a critical role in promoting metastasis in prostate cancer. Knocking down ELOVL5 leads to the accumulation of malonyl-CoA, which inhibits fatty acid oxidation in mitochondria. This study highlights the importance of fatty acid elongation in regulating cell viability and provides a potential target for prostate cancer treatment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)
Article
Biochemistry & Molecular Biology
Zan Zhou, Wen-jun Jiang, Li Li, Jun-qiang Si
Summary: This study investigates the effect of noise exposure on cognitive function in mice and explores the underlying molecular mechanisms. The findings suggest that noise exposure leads to increased inflammation, increased phosphorylation of Tau protein, and decreased levels of postsynaptic density protein, resulting in cognitive impairment.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2024)