Article
Multidisciplinary Sciences
Shokoofeh Shamsi, Lachlan Sibraa, Xiaocheng Zhu, Diane P. Barton
Summary: The study identified Temnocephalidae species in Macrobrachium australiense and found that 96.3% of the prawns showed signs of infection. The phylogenetic tree showed that the Temnocephalidae species in this study grouped separately from those reported in Australia.
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Warachin Gangnonngiw, Malinee Bunnontae, Pattanapon Kayansamruaj, Saengchan Senapin, Jiraporn Srisala, Timothy W. Flegel, Kanokpan Wongprasert
Summary: A novel ssDNA virus, Macrobrachium hepatopancreatic bidnavirus (MHBV), was identified in giant freshwater prawn using metagenomic analysis and de novo assembly. This virus showed circular, eosinophilic inclusions in the nuclei of hepatopancreatic cells. The MHBV genome closely matched the polymerase B gene in the V1 fragment of Bombyx mori bidensovirus 2 (BmBDV2) and belongs to the family Bidnaviridae.
Article
Environmental Sciences
Qun Jiang, Ziyan Jiang, Shiqi Ao, Xiaojian Gao, Xinhai Zhu, Zirui Zhang, Xiaojun Zhang
Summary: The study assessed the toxicity of deltamethrin on Macrobrachium rosenbergii, revealing tissue damage and oxidative stress at high concentrations, with persistent harm even after decontamination.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Fisheries
Jonathan Molcho, Rivka Manor, Maayan Shamsian, Gurucharan Sudarshan, Rivka Ofir, Danit Parker, Simy Weil, Hanin Wattad, Emily Hayun, Tom Levy, Eliahu D. Aflalo, Ayal Hendel, Amir Sagi
Summary: This study successfully established two CRISPR platforms for the giant freshwater prawn Macrobrachium rosenbergii and achieved high gene editing efficiency through optimization. Editing efficiency was examined and reached as high as 100% in embryos, while the maximum efficiency obtained in primary embryonic cell cultures was 64%. Additionally, a striking difference in deletion patterns around the Cas9 cut site was observed between the two systems, indicating distinct repair mechanisms that require further clarification. The investigation of the Pax6 transcription factor provided a phenotypic proof of concept by showing clear effects on eye development in edited embryos and larvae. This study lays down the foundations for precise functional genomic research and genome editing applications in crustacean species for both aquaculture and sustainable biocontrol, offering opportunities for the creation of selected crustacean lines with distinct attributes.
Article
Biochemical Research Methods
Xi-Lian Li, Qiang Gao, Pei-jing Shen, Yu-fei Zhang, Wen-ping Jiang, Zhen-yuan Huang, Fei Peng, Zhi-min Gu, Xue-Feng Chen
Summary: This study conducted proteomic analysis on iron prawn and identified a large number of differently expressed proteins, which play various functional roles in the severe growth retardation of iron prawn.
JOURNAL OF PROTEOMICS
(2021)
Article
Physiology
Yakun Wang, Jie Wei, Kunhao Hong, Nan Zhou, Xiaoli Liu, Xiaoyou Hong, Wei Li, Jian Zhao, Chen Chen, Liang Wu, Lingyun Yu, Xinping Zhu
Summary: This study revealed the molecular mechanism of salinity response in larvae of the giant freshwater prawn through transcriptome analysis. Differential expression of genes involved in osmoregulation, energy metabolism, molting, and immune response was observed in larvae cultured at different salinities.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Zoology
Nukul Saengphan, Bhinyo Panijpan, Saengchan Senapin, Parames Laosinchai, Auaree Suksomnit, Kornsunee Phiwsaiya
Summary: A new species of small freshwater prawn was discovered in the upper southern peninsula of Thailand. This new species, M. prachuapense sp. nov., has distinct characteristics that differentiate it from closely related species, and phylogenetic analysis confirms its unique status.
Article
Environmental Sciences
Md Ashekur Rahman, Md Yeamin Hossain, Mostafa A. R. Hossain, Zoarder Faruque Ahmed, Ahmed Jaman, Jun Ohtomi
Summary: This study explored the effects of climate change on the reproductive activity of the monsoon river prawn in the Ganges River basin of Bangladesh. The study found a significant correlation between rainfall and the spawning season, with rainfall impacting the number of ovigerous females and fecundity. The findings suggest that the spawning season of the monsoon river prawn may shift due to climate change.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Fisheries
Quanxin Gao, Jinping Luo, Peimin Liu, Shaokui Yi, Qiongying Tang, Jingfen Li, Zhenglong Xia, Guoliang Yang
Summary: This study explored the relationship between gut bacterial community composition and phenotypic traits in different morphotypes of giant freshwater prawn. The results showed that Lactococcus garvieae was the dominant bacterial species and the relative abundance of gut microbiota differed significantly among different morphotypes. Furthermore, there were significant associations between gut microbiota and phenotypic traits.
Article
Environmental Sciences
Bo Liu, Changyou Song, Qiang Gao, Bo Liu, Qunlan Zhou, Cunxin Sun, Huimin Zhang, Mingyang Liu, Dawit Adisu Tadese
Summary: This study found that maternal and environmental microbes have significant impacts on the embryonic and larval microbiota of Macrobrachium rosenbergii. The microbial colonization in the embryonic stage was directly related to the maternal prawn's microbiota, while the post-larval stage microbiota was more influenced by the environment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Fisheries
Lindan Sun, Feng Lin, Binbin Sun, Zhendong Qin, Keping Chen, Lijuan Zhao, Jun Li, Yulei Zhang, Li Lin
Summary: This study found that Scutellaria polysaccharide (SPS) extracted from the Chinese medicinal herb Scutellaria baicalensis can regulate the immune capacity and enhance the antioxidant capacity of the giant freshwater prawn (Macrobrachium rosenbergii), thus improving its survival rate.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Baharuddin Haslawati, Ibrahim Saadiah, Razman Pahri Siti-Dina, Murnira Othman, Mohd Talib Latif
Summary: This study used a cradle-to-farm Life Cycle Assessment to assess the potential environmental impacts of Macrobrachium rosenbergii farming in Malaysia. The study found that the farming phase had the largest impact on human carcinogenic toxicity and freshwater ecotoxicity. Overall, M. rosenbergii farming was found to have a low environmental impact, but further research on other aspects of sustainability is needed.
Review
Fisheries
Kianann Tan, Weimin Wang
Summary: This article reviews factors influencing the embryonic development of M. rosenbergii and key points in larval rearing that can promote its growth and survival. It also covers gonadal development and functional genes associated with the reproductive process. Understanding these topics is of importance for the culture and reproductive success of M. rosenbergii.
Review
Fisheries
Bindu R. Pillai, Raul W. Ponzoni, Kanta Das Mahapatra, Debabrata Panda
Summary: The giant freshwater prawn is a popular tropical species widely cultured across the world with high market demand and attractive prices. Several countries have started selective breeding programmes for this species focused on growth rate, which has resulted in increased edible meat without negative effects on flesh quality attributes. Despite challenges, ongoing research aims to improve productivity in this species.
REVIEWS IN AQUACULTURE
(2022)
Article
Agriculture, Dairy & Animal Science
Jitao Xia, Chong Wang, Liang Yao, Wei Wang, Wenxiu Zhao, Tianchang Jia, Xingtong Yu, Guoliang Yang, Qingli Zhang
Summary: This study detected the presence of Covert mortality nodavirus (CMNV) in farmed giant freshwater prawn (Macrobrachium rosenbergii) in Jiangsu, China using TaqMan RT-qPCR. The study identified the intestine, gill, hepatopancreas, and ovary as target organs of CMNV infection in giant freshwater prawn. In addition, the study observed CMNV-like particles in the hepatopancreas and gill tissues. These findings further expand our knowledge on CMNV and provide new insights for investigating the pathogenic factors of giant freshwater prawn disease.
Article
Infectious Diseases
Yuttapong Thawornwattana, Ha Thanh Dong, Kornsunee Phiwsaiya, Pakkakul Sangsuriya, Saengchan Senapin, Pakorn Aiewsakun
Summary: Tilapia lake virus (TiLV) is an emerging virus that rapidly spread worldwide over the past 6 years, its origin and epidemiology characteristics are still largely unknown.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Fisheries
Naruporn Rungrueng, Watcharachai Meemetta, Kornsunee Phiwsaiya, Ha Thanh Dong, Wattana Panphut, Saengchan Senapin
Summary: Accuracy is key in PCR diagnostics, with inconsistent results observed when changing Taq DNA polymerase brands. Testing of 6 primer sets for aquaculture viruses showed differences in specificity and sensitivity, highlighting the potential improvement of detection sensitivity by adding ammonium sulfate to reaction buffers.
AQUACULTURE INTERNATIONAL
(2021)
Article
Fisheries
Nguyen Dinh-Hung, Pattiya Sangpo, Thanapong Kruangkum, Pattanapon Kayansamruaj, Tilladit Rung-ruangkijkrai, Saengchan Senapin, Channarong Rodkhum, Ha Thanh Dong
Summary: This study identified the distribution of TiLV in the entire brain of experimentally infected Nile tilapia, showing that the virus was broadly distributed throughout the forebrain and hindbrain. The virus may enter the brain through the circulatory system and induce brain dysfunction. Understanding the pattern of viral localization in the brain could help elucidate the neurological disorders of the diseased fish.
JOURNAL OF FISH DISEASES
(2021)
Article
Zoology
Nukul Saengphan, Bhinyo Panijpan, Saengchan Senapin, Parames Laosinchai, Auaree Suksomnit, Kornsunee Phiwsaiya
Summary: A new species of small freshwater prawn was discovered in the upper southern peninsula of Thailand. This new species, M. prachuapense sp. nov., has distinct characteristics that differentiate it from closely related species, and phylogenetic analysis confirms its unique status.
Article
Fisheries
Jantana Kampeera, Sirintip Dangtip, Rapheephat Suvannakad, Pakapreud Khumwan, Saengchan Senapin, Wansika Kiatpathomchai
Summary: Tilapia is a globally significant aquaculture species facing mortality issues due to infectious pathogens like TiLV. A novel colorimetric assay based on RT-LAMP and AuNP has been developed for rapid onsite detection, showing high agreement with conventional PCR methods recommended by OIE but requiring significantly less time to perform.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Jerome Delamare-Deboutteville, Suwimon Taengphu, Han Ming Gan, Pattanapon Kayansamruaj, Partho Pratim Debnath, Andrew Barnes, Shaun Wilkinson, Minami Kawasaki, Chadag Vishnumurthy Mohan, Saengchan Senapin, Ha Thanh Dong
Summary: This study successfully demonstrated a rapid diagnostic and genotyping method for tilapia lake virus using a combination of specific PCR assay and ONT-based amplicon sequencing, with high accuracy and operational feasibility. The approach is suitable for deployment in regional aquatic animal health diagnostic laboratories in low- and medium-income countries, allowing for fast identification and genotyping of emerging infectious pathogens from field samples within a single day.
JOURNAL OF FISH DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
Wasusit Somsoros, Takeshi Sangawa, Katsuki Takebe, Jakrada Attarataya, Kanokpan Wongprasert, Saengchan Senapin, Triwit Rattanarojpong, Mamoru Suzuki, Pongsak Khunrae
Summary: The study revealed the crystal structure of the C-terminal domain of VP37 from WSSV, showing a trimeric assembly, sulphate binding sites, and the importance of these sites in binding to heparin. Mutagenesis of R213 and K257 resulted in significant loss of heparin binding activity, further demonstrating the essential role of these sites for VP37 binding to sulphate molecules.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Fisheries
Thanwarat Sukonta, Saengchan Senapin, Watcharachai Meemetta, Thawatchai Chaijarasphong
Summary: A novel detection platform for SDDV based on CRISPR-Cas12a and recombinase polymerase amplification technology has been developed, showing high sensitivity and specificity for rapid and accurate diagnosis of SDDV. This user-friendly method can be completed within an hour at room temperature, potentially serving as a valuable tool for combating large-scale epidemics in fish farming communities.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Partho Pratim Debnath, Nguyen Dinh-Hung, Suwimon Taengphu, Vuong Viet Nguyen, Jerome Delamare-Deboutteville, Saengchan Senapin, Chadag Vishnumurthy Mohan, Ha Thanh Dong, Channarong Rodkhum
Summary: Sixteen countries have reported the presence of tilapia lake virus (TiLV), an emerging pathogen affecting tilapia. In Bangladesh, 20% of farms tested positive for TiLV in tilapia, while co-cultivated fish species and invertebrates did not show signs of TiLV infection. Experimental infection only showed clinical signs in Nile tilapia, suggesting that species in polycultures may not be susceptible to TiLV.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Ha Thanh Dong, Pattiya Sangpo, Le Thanh Dien, Thao Thu Mai, Nguyen Vu Linh, Jorge del-Pozo, Krishna R. Salin, Saengchan Senapin
Summary: CAIB in the pancreas of adult Nile tilapia infected with TiPV serves as a diagnostic feature, indicating exclusive replication of TiPV in pancreatic tissues, often accompanied by co-infection with Streptococcus agalactiae and secondary granulomas.
JOURNAL OF FISH DISEASES
(2022)
Review
Fisheries
Ha Thanh Dong, Thawatchai Chaijarasphong, Andrew C. Barnes, Jerome Delamare-Deboutteville, Peiyu Alison Lee, Saengchan Senapin, Chadag Vishnumurthy Mohan, Kathy F. J. Tang, Sharon E. McGladdery, Melba G. Bondad-Reantaso
Summary: The article "Diagnostics for diseases of tilapia: From basic to molecular diagnosis" explores the importance of the three levels of diagnostics for diseases of tilapia, particularly the frequently overlooked basic procedures. The article also covers current and emerging molecular diagnostic technologies for tilapia pathogens, and discusses future developments for transferring these technologies to farmers and stakeholders for a sustainable aquatic food system transformation.
REVIEWS IN AQUACULTURE
(2023)
Article
Fisheries
Ramida Phasunon, Suwimon Taengphu, Wattana Panphut, Satid Chatchaiphan, Ha Thanh Dong, Saengchan Senapin
Summary: A newly developed probe-based qPCR method for the detection of S. iniae was reported in this study. The method demonstrated high specificity and sensitivity, and can be applied for the diagnosis and surveillance of S. iniae infections in fish populations.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Nguyen Vu Linh, Nareerat Khongcharoen, Dinh-Hung Nguyen, Le Thanh Dien, Naruporn Rungrueng, Chayuda Jhunkeaw, Pattiya Sangpo, Saengchan Senapin, Toungporn Uttarotai, Wattana Panphut, Sophie St-Hilaire, Hien Van Doan, Ha Thanh Dong
Summary: This study investigated the effects of oxygen nanobubble treatment on Nile tilapia. The results showed that both short-term and long-term exposure to high oxygen levels did not have obvious adverse effects on the fish, although there were some changes in the gut microbiome.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Onanong Charoenwai, Saengchan Senapin, Ha Thanh Dong, Molruedee Sonthi
Summary: Non-destructive sampling methods are beneficial for detecting and monitoring viral pathogens in economically important farmed fish. Blood was found to be the best non-destructive sample source for detecting scale drop disease virus in farmed Asian sea bass.
JOURNAL OF FISH DISEASES
(2021)
