Article
Genetics & Heredity
Anura Shodhan, Martin Xaver, David Wheeler, Michael Lichten
Summary: The axis protein Hop1 plays a crucial role in promoting double-strand break formation and homologous recombination during meiotic recombination, while other factors may determine the specific recombination pathways involved.
Article
Biochemistry & Molecular Biology
Chao Yang, Kostika Sofroni, Yuki Hamamura, Bingyan Hu, Hasibe Tuncay Elbasi, Martina Balboni, Lei Chu, Dagmar Stang, Maren Heese, Arp Schnittger
Summary: The ASY1 remodeling complex is assembled differently in terms of timing and location. PCH2 and COMET interact in the cytoplasm during early meiosis, but PCH2 is recruited by ZYP1 and brought to the ASY1-bound COMET to ensure the timely removal of ASY1 during chromosome synapsis.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Plant Sciences
Maria Cuacos, Christophe Lambing, Miguel Pachon-Penalba, Kim Osman, Susan J. Armstrong, Ian R. Henderson, Eugenio Sanchez-Moran, F. Christopher H. Franklin, Stefan Heckmann
Summary: In Brassica rapa, axis formation and remodelling are crucial for meiotic fidelity, including synapsis and crossover (CO) formation. Mutants of ASY1 and PCH2 show altered distribution of CO, with asy1 plants being sterile and pch2 plants being semi-sterile, making PCH2 a potential target for breeding programs.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biology
Dorota Rousova, Vaishnavi Nivsarkar, Veronika Altmannova, Vivek B. Raina, Saskia K. Funk, David Liedtke, Petra Janning, Franziska Mueller, Heidi Reichle, Gerben Vader, John R. Weir
Summary: This study focuses on the foundational DSB factor Mer2 and reveals its interaction with nucleosomes and a chromosomal axis factor, as well as its crucial role in DSB activity. By bridging key protein complexes involved in the initiation of meiotic recombination, Mer2 is established as a keystone of the DSB machinery.
Article
Genetics & Heredity
Anna D. Russo, Stefani Giacopazzi, Alison P. Deshong, Malaika E. Menon, Valery P. Ortiz, Kaori E. Ego, Kevin P. Corbett, Needhi E. Bhalla
Summary: Sexual reproduction relies on the coordination of various meiotic events, such as pairing, synapsis, and recombination. The enzyme PCH-2 plays a crucial role in regulating these events by interacting with different members of the meiotic HORMAD protein family in the nematode C. elegans. This coordination ensures the fidelity of meiotic chromosome segregation.
Article
Cell Biology
Xuan Yang, Binyuan Zhai, Shunxin Wang, Xiangfei Kong, Yingjin Tan, Lin Liu, Xiao Yang, Taicong Tan, Shuxian Zhang, Liangran Zhang
Summary: RNA-DNA hybrids formed at ssDNA ends of DSBs actively regulate meiotic recombination in budding yeast, affecting the efficiency of sporulation and spore viability. The accumulation of RNA-DNA hybrids competes with Rad51/Dmc1, impairs homolog bias, and decreases crossover and noncrossover recombination.
Article
Biochemistry & Molecular Biology
Ying Wang, Binyuan Zhai, Taicong Tan, Xiao Yang, Jiaming Zhang, Meihui Song, Yingjin Tan, Xuan Yang, Tingting Chu, Shuxian Zhang, Shunxin Wang, Liangran Zhang
Summary: Meiotic recombination is regulated by chromosome organization, with the NuA4 complex catalytic subunit Esa1 playing a crucial role in chromosome axis length regulation and recombination frequency. Depletion of Esa1 affects chromosome structure and downregulates specific meiotic genes, leading to defects in meiotic processes.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Taicong Tan, Yingjin Tan, Ying Wang, Xiao Yang, Binyuan Zhai, Shuxian Zhang, Xuan Yang, Hui Nie, Jinmin Gao, Jun Zhou, Liangran Zhang, Shunxin Wang
Summary: This study reveals that negative supercoils play a crucial role in connecting interference and crossover interference in meiosis, and top2 gene mutations affect the accumulation and relief of negative supercoils, thereby regulating the strength of crossover interference.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Andrology
Feng-Guo Zhang, Rui-Rui Zhang, Jin-Min Gao
Summary: The synaptonemal complex (SC) is a proteinaceous macromolecular structure that assembles between paired homologous chromosomes during meiosis in various eukaryotes, playing critical roles in accurate meiotic chromosome segregation. Recent studies using super-resolution microscopy have provided insights into the structure and regulation of the SC, shedding light on its biological functions.
ASIAN JOURNAL OF ANDROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Takeshi Sakuno, Sanki Tashiro, Hideki Tanizawa, Osamu Iwasaki, Da-Qiao Ding, Tokuko Haraguchi, Ken-ichi Noma, Yasushi Hiraoka
Summary: The Rec8-dependent axis-loop structure formed during meiotic prophase is essential for LinE assembly and facilitates meiotic recombination of homologous chromosomes, independent of its role in sister chromatid cohesion.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Florencia Pratto, Kevin Brick, Gang Cheng, Kwan-Wood Gabriel Lam, Jeffrey M. Cloutier, Daisy Dahiya, Stephen R. Wellard, Philip W. Jordan, R. Daniel Camerini-Otero
Summary: This passage discusses the distribution of genetic recombination in the genome, the role of the PRDM9 protein, and the impact of meiotic replication on recombination. Research findings show that meiotic DNA replication is distinct from replication in somatic cells, and this has important implications for understanding genetic recombination and the recombination potential of individual chromosomes.
Article
Genetics & Heredity
Krishnaprasad G. Nandanan, Sagar Salim, Ajith Pankajam, Miki Shinohara, Gen Lin, Parijat Chakraborty, Amamah Farnaz, Lars M. Steinmetz, Akira Shinohara, Koodali T. Nishant
Summary: The Msh4-Msh5 complex in baker's yeast plays a vital role in meiotic crossovers and is found to associate specifically with DSB hotspots, chromosome axes, and centromeres. Its binding is enhanced by DSB formation and resection, and is correlated to DSB frequency, as well as being influenced by chromosome size and density of crossovers. These findings shed light on how Msh4-Msh5 works with other pro-crossover factors to ensure crossover formation.
Article
Genetics & Heredity
Fabien Dutreux, Abhishek Dutta, Emilien Peltier, Sabrina Bibi-Triki, Anne Friedrich, Bertrand Llorente, Joseph Schacherer
Summary: In this study, the non-model budding yeast Lachancea waltii was used to investigate meiotic recombination. It was found that L. waltii frequently lacks crossover, suggesting the existence of an alternative mechanism for gamete formation. The interference between meiotic crossovers in L. waltii was reduced compared to S. cerevisiae, and crossover hotspots were not conserved between Lachancea and Saccharomyces clades. This study demonstrates that meiotic recombination can evolve at a limited evolutionary scale within budding yeasts.
Review
Genetics & Heredity
Meret Arter, Scott Keeney
Summary: This article discusses the process of recombination in meiosis, noting its broad conservation but specific differences between different taxa. The authors highlight the tension between functional conservation and rapid evolutionary change, and explore the important factors that shape our understanding of molecular mechanisms in reproductive biology.
NATURE REVIEWS GENETICS
(2023)
Article
Genetics & Heredity
Christophe Lambing, Pallas Kuo, Jaeil Kim, Kim Osman, Amy Leanne Whitbread, Jianhua Yang, Kyuha Choi, F. Chris H. Franklin, Ian R. Henderson
Summary: This study provides mechanistic insights into the control of meiotic DSB formation and reveals diverse functional interactions between SPO11-1, PRD3 and the chromosome axis.
Editorial Material
Biochemistry & Molecular Biology
Akira Yamashita, Ryo Kariyazono, Yoshinori Watanabe
Article
Multidisciplinary Sciences
Hayato Hirai, Kunio Arai, Ryo Kariyazono, Masayuki Yamamoto, Masamitsu Sato
Article
Plant Sciences
Ryo Kariyazono, Takashi Osanai
Summary: This study identified the binding sites of SigE and SigA using ChIP-seq analysis and found that they shared most of their binding sites, but SigE exclusively occupied the promoters of the SigE regulon.