Article
Plant Sciences
Thais R. Boufleur, Nelson S. S. Massola Jr, Sioly Becerra, Elena Baraldi, Lillian B. J. Bibiano, Serenella A. A. Sukno, Michael R. R. Thon, Riccardo Baroncelli
Summary: This study investigates the response of soybean to different strains of C. truncatum and reveals that different soybean cultivars exhibit varying levels of susceptibility. The study also identifies genes related to the plant immune system that play important roles in defense against soybean anthracnose.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Infectious Diseases
Heng Kang Ng, Suat Moi Puah, Cindy Shuan Ju Teh, Nuryana Idris, Kek Heng Chua
Summary: This study investigated the transcriptomic profiles of pellicle cells from carbapenem-resistant Acinetobacter baumannii strains. The analysis identified 366 differentially expressed genes (DEGs) in pellicle cells, including potential genes involved in fatty acid degradation (ABUW_1624, ABUW_1550) and a methionine transporter system gene (ABUW_2820). Upregulation of two-component systems (CusSR, KdpDE) related to copper and potassium ion regulation during CRAB pellicle formation was also observed. These findings provide insights into gene expression regulation in CRAB pellicle formation and potential targets for eradicating CRAB infections.
Article
Plant Sciences
Eugene Koh, William Goh, Irene Julca, Erielle Villanueva, Marek Mutwil
Summary: The Plant Expression Omnibus (PEO) is a web application that provides biologists with access to gene expression insights across over 100 plant species. Users can explore the expression patterns of genes across different organs and discover functional annotations.
Article
Food Science & Technology
Xiaojun Zhang, Ning Tang, Huijie Zhang, Cunkun Chen, Li Li, Chenghu Dong, Yongqiang Cheng
Summary: Ozone treatment delays postharvest cantaloupe decay and improves its quality by regulating gene expression in peel and pulp, maintaining firmness and reducing ethylene production. It also affects phenylalanine metabolic pathway, promoting secondary metabolism in pulp.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Immunology
Feng-Jie Su, Thirunavukkarasu Periyasamy, Meei-Mei Chen
Summary: This study found that Lactococcus garvieae infection induces a different immune response compared to planktonic bacterial infection. Preventing biofilm formation may be a new strategy for controlling bacterial infection in aquaculture.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tian Zhao, Jiaqi Chen, Shuai Liu, Jie Yang, Juan Wu, Leiying Miao, Weibin Sun
Summary: Periodontitis is a chronic infectious disease that can lead to various dental problems and even tooth loss. The dental plaque biofilm is a crucial factor in the development of periodontitis. Fusobacterium nucleatum plays an important role in the structure and ecology of dental plaque biofilms. Understanding the molecular mechanism of F. nucleatum during biofilm development is essential for controlling periodontitis. This study used RNA-sequencing and quantitative reverse transcriptase PCR (RT-qPCR) to analyze the gene expression profiles of F. nucleatum in the planktonic and biofilm phases. The results revealed significant differences in gene expression between the two states, with several genes related to metabolism, cell growth, division, oxidative stress, virulence, and oral malodor being differentially expressed. Additionally, the study identified lysine as a potential antibacterial and antibiofilm agent against F. nucleatum.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Immunology
Akinsola Oyelakin, Erich Horeth, Eun-Ah Christine Song, Sangwon Min, Monika Che, Brandon Marzullo, Christopher J. Lessard, Astrid Rasmussen, Lida Radfar, R. Hal Scofield, David M. Lewis, Donald U. Stone, Kiely Grundahl, Scott S. De Rossi, Zoya Kurago, A. Darise Farris, Kathy L. Sivils, Satrajit Sinha, Jill M. Kramer, Rose-Anne Romano
Summary: Primary Sjogren's syndrome is a systemic autoimmune disease primarily affecting women, leading to loss of saliva and tears. Currently, there is no effective cure due to limited understanding of disease etiology and pathology.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Erich Horeth, Akinsola Oyelakin, Eun-Ah Christine Song, Monika Che, Jonathan Bard, Sangwon Min, Jeremy Kiripolsky, Jill M. Kramer, Satrajit Sinha, Rose-Anne Romano
Summary: Sjogren's Syndrome is a chronic autoimmune disease affecting salivary and lacrimal glands. RNA sequencing and single-cell RNA sequencing have revealed important pathways and regulatory networks in the pathobiology of SS, as well as the diversity in activated state of salivary gland epithelial cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Orthopedics
Nathaniel P. Disser, Alexander N. Piacentini, Andrea J. De Micheli, Martin M. Schonk, Vincent J. H. Yao, Xiang-Hua Deng, David J. Oliver, Scott A. Rodeo
Summary: This study investigated the transcriptomic and mechanical properties of a single Achilles tendon and found region-specific gene expression signatures and mechanical properties. The proximal region displayed gene expression resembling a tendon phenotype and increased expression of nerve cell markers, while the distal region showed increased expression of genes involved in extracellular matrix synthesis and remodeling, immune cell regulation, and a phenotype similar to cartilage and bone. There was a significant increase in Young modulus from the proximal to middle region and an additional increase from the middle to distal region.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2022)
Article
Dentistry, Oral Surgery & Medicine
E. Horeth, J. Bard, M. Che, T. Wrynn, E. A. C. Song, B. Marzullo, M. S. Burke, S. Popat, T. Loree, J. Zemer, J. L. Tapia, J. Frustino, J. M. Kramer, S. Sinha, R. A. Romano
Summary: This study comprehensively characterizes the molecular features and expression profiles of human submandibular gland cells using single-cell and bulk RNA sequencing. It reveals a variety of cell types and provides insights into cell-cell communication and the dynamic process of differentiation.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Patrick Danaher, Youngmi Kim, Brenn Nelson, Maddy Griswold, Zhi Yang, Erin Piazza, Joseph M. Beechem
Summary: The paper introduces SpatialDecon, a method for deconvolution and quantification of cell types in spatial transcriptomics data, demonstrating its application in analyzing immune response heterogeneity in cancer. SpatialDecon incorporates advancements in gene expression deconvolution and provides cell abundance estimates with high spatial resolution.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Thidathip Wongsurawat, Piroon Jenjaroenpun, Visanu Wanchai, Intawat Nookaew
Summary: Direct sequencing of single molecules through nanopores allows accurate quantification and full-length characterization of native RNA or cDNA without amplification. This study compares the characteristics and differences between nanopore-based native RNA and cDNA sequencing methods, and evaluates their performance using Saccharomyces cerevisiae as a model organism. The findings suggest that while there are differences in sequence characteristics, the two approaches yield comparable results in differential gene expression analysis. The study also highlights the underestimation of RNA modification in direct RNA sequencing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Pranjali A. Gedam, Kiran Khandagale, Dhananjay Shirsat, A. Thangasamy, Onkar Kulkarni, Abhijeet Kulkarni, Swaranjali S. Patil, Vitthal T. Barvkar, Vijay Mahajan, Amar Jeet Gupta, Kiran P. Bhagat, Yogesh P. Khade, Major Singh, Suresh Gawande
Summary: This study identified differentially expressed genes (DEGs) through transcriptome analysis in two onion genotypes (Acc. 1666 and W-344) with contrasting responses to waterlogging stress. The proteins coded by these DEGs regulate key biological processes such as phytohormone production, antioxidant enzymes, programmed cell death, and energy production. The results provide valuable molecular information for improving stress tolerance and developing waterlogging tolerant onion varieties.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Food Science & Technology
Yibin Xu, Yuanyuan Wang, Xiaoqing Ding, Jiangshui Wang, Xiuan Zhan
Summary: This study demonstrates the antibacterial and antibiofilm properties of reuterin derived from L. reuteri strains. Reuterin exerts its bactericidal activity through ROS production. Furthermore, co-cultures with glycerol enhance the inhibitory effects of LR 21 on Cp 13124, reducing bacterial count and the production of virulence factors.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Cell Biology
Shouguo Gao, Zhijie Wu, Jeerthi Kannan, Liza Mathews, Xingmin Feng, Sachiko Kajigaya, Neal S. Young
Summary: The study revealed evolutionary conservation in the hematopoietic systems of mice and humans, extending to cell types, gene expression, and regulatory elements.
Article
Veterinary Sciences
Kriengwich Limpavithayakul, Nataya Charoenvisal, Somsak Pakpinyo
Summary: The PCR-RFLP molecular assay was verified as a convenient technique for detecting the MS-H vaccine strain, and the immune response mechanisms in vaccinated layer-type chickens were characterized. Vaccination stimulated immune response mechanisms in chickens with no significant difference between single and double doses.
Article
Veterinary Sciences
Doan Hoang Phu, Ruethai Narinthorn, Nguyen Thi Nhung, Niwat Chansiripornchai, Patrick J. Blackall, Conny Turni, Juan Carrique-Mas, Thotsapol Thomrongsuwannakij
Summary: Chicken in southern Thailand has different prevalence and resistance patterns of Campylobacter. Commercial broilers exhibit higher resistance to quinolones and lower resistance to macrolides and tetracycline compared to native chickens. Resistance patterns are more consistent within farms, and commercial broilers show higher homogeneity. Phenotypic and genotypic resistance have partial agreement among isolates.
Article
Veterinary Sciences
Sanjiv Kumar, Madhurendu Kumar Gupta, Sanjit Kumar, Tanmoy Rana
Summary: This study evaluated the relative efficacy of allopurinol and febuxostat, two important anti-gout agents, in controlling hyperuricemia/gout using a poultry model. The results showed that both allopurinol and febuxostat have definite potential for ameliorating hyperuricemia and gout in poultry, with febuxostat showing better effects compared to allopurinol.
Article
Veterinary Sciences
Robert Jan Molenaar, Remco Dijkman, Christiaan ter Veen, Annet Heuvelink, Fiona van Kaam, Marieke Augustijn, Anneke Feberwee
Summary: This report describes an outbreak of Salmonella Pullorum (SP) in a Dutch laying flock, causing increased mortality, decreased egg production, and neurological signs. SP was frequently isolated from various organs, and immunostaining showed cerebral granulomas in birds with neurological signs. Vaccination did not affect the detection of SP antibodies. Some clinically healthy birds had ovarian lesions associated with SP contact, but carrier status could not be confirmed. Phylogenetic analysis showed clonality of the SP isolates within the outbreak.
Article
Veterinary Sciences
Yingfei Sun, Yingjie Wang, Lulu Wang, Mengyun Zou, Xiuli Peng
Summary: This study investigates the regulation of miR-33-5p gene expression during MG infection. The researchers found an upregulation in pri-miR-33-5p and pre-miR-33-5p expression, indicating transcriptional regulation. They identified a binding motif for STAT5 in the miR-33-5p promoter element and showed that STAT5 modulates miR-33-5p transcription in response to MG infection. The overexpression of STAT5 led to increased miR-33-5p expression while suppressing JNK1 expression.