Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging

Multiplex quantitative analysis of tumor-infiltrating lymphocytes and immunotherapy outcome in metastatic melanoma

(2019) Pok Fai Wong et al.   CLINICAL CANCER RESEARCH

Interpreting heterogeneity in intestinal tuft cell structure and function

(2018) Amrita Banerjee et al.   JOURNAL OF CLINICAL INVESTIGATION

Cluster Size and Quinary Structure Determine the Rheological Effects of Antibody Self-Association at High Concentrations

(2018) Wenhua Wang et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Generation and validation of homozygous fluorescent knock-in cells using CRISPR–Cas9 genome editing

(2018) Birgit Koch et al.   Nature Protocols

Mass cytometry analysis of immune cells in the brain

(2018) Ben Korin et al.   Nature Protocols

Multiplexed immunofluorescence reveals potential PD-1/PD-L1 pathway vulnerabilities in craniopharyngioma

(2018) Shannon Coy et al.   NEURO-ONCOLOGY

Meningioma transcription factors link cell lineage with systemic metabolic cues

(2018) Ziming Du et al.   NEURO-ONCOLOGY

Atezolizumab for First-Line Treatment of Metastatic Nonsquamous NSCLC

(2018) Mark A. Socinski et al.   NEW ENGLAND JOURNAL OF MEDICINE

Neoadjuvant PD-1 Blockade in Resectable Lung Cancer

(2018) Patrick M. Forde et al.   NEW ENGLAND JOURNAL OF MEDICINE

Emerging biomarkers for immune checkpoint inhibition in lung cancer

(2018) George Cyriac et al.   SEMINARS IN CANCER BIOLOGY

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes

(2018) Jia-Ren Lin et al.   eLife

Simultaneous Multiplexed Imaging of mRNA and Proteins with Subcellular Resolution in Breast Cancer Tissue Samples by Mass Cytometry

(2018) Daniel Schulz et al.   Cell Systems

Unsupervised Trajectory Analysis of Single-Cell RNA-Seq and Imaging Data Reveals Alternative Tuft Cell Origins in the Gut

(2018) Charles A. Herring et al.   Cell Systems

Deep Profiling of Mouse Splenic Architecture with CODEX Multiplexed Imaging

(2018) Yury Goltsev et al.   CELL

A Structured Tumor-Immune Microenvironment in Triple Negative Breast Cancer Revealed by Multiplexed Ion Beam Imaging

(2018) Leeat Keren et al.   CELL

Fundamental Mechanisms of Immune Checkpoint Blockade Therapy

(2018) Spencer C. Wei et al.   Cancer Discovery

A Cancer Cell Program Promotes T Cell Exclusion and Resistance to Checkpoint Blockade

(2018) Livnat Jerby-Arnon et al.   CELL

New tools for pathology: a user's review of a highly multiplexed method for in situ analysis of protein and RNA expression in tissue

(2018) Jérémie Decalf et al.   JOURNAL OF PATHOLOGY

Avelumab for patients with previously treated metastatic or recurrent non-small-cell lung cancer (JAVELIN Solid Tumor): dose-expansion cohort of a multicentre, open-label, phase 1b trial

(2017) James L Gulley et al.   LANCET ONCOLOGY

Rapid Sequential in Situ Multiplexing with DNA Exchange Imaging in Neuronal Cells and Tissues

(2017) Yu Wang et al.   NANO LETTERS

Elements of cancer immunity and the cancer–immune set point

(2017) Daniel S. Chen et al.   NATURE

Data-analysis strategies for image-based cell profiling

(2017) Juan C Caicedo et al.   NATURE METHODS

histoCAT: analysis of cell phenotypes and interactions in multiplex image cytometry data

(2017) Denis Schapiro et al.   NATURE METHODS

First-Line Nivolumab in Stage IV or Recurrent Non–Small-Cell Lung Cancer

(2017) David P. Carbone et al.   NEW ENGLAND JOURNAL OF MEDICINE

Technology Feature | Agreeable antibodies: Antibody validation challenges and solutions

(2017) Alan Dove   SCIENCE

Quantitative Multiplex Immunohistochemistry Reveals Myeloid-Inflamed Tumor-Immune Complexity Associated with Poor Prognosis

(2017) Takahiro Tsujikawa et al.   Cell Reports

Coinhibitory Pathways in Immunotherapy for Cancer

(2016) Susanne H. Baumeister et al.   Annual Review of Immunology

Combination Therapy with Anti-PD-1, Anti-TIM-3, and Focal Radiation Results in Regression of Murine Gliomas

(2016) Jennifer E. Kim et al.   CLINICAL CANCER RESEARCH

Development and Validation of a Deep Learning Algorithm for Detection of Diabetic Retinopathy in Retinal Fundus Photographs

(2016) Varun Gulshan et al.   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

Machine learning and computer vision approaches for phenotypic profiling

(2016) Ben T. Grys et al.   JOURNAL OF CELL BIOLOGY

Automated mapping of phenotype space with single-cell data

(2016) Nikolay Samusik et al.   NATURE METHODS

A proposal for validation of antibodies

(2016) Mathias Uhlen et al.   NATURE METHODS

High-performance multiplexed fluorescence in situ hybridization in culture and tissue with matrix imprinting and clearing

(2016) Jeffrey R. Moffitt et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Visualization and analysis of gene expression in tissue sections by spatial transcriptomics

(2016) P. L. Stahl et al.   SCIENCE

Large molecular systems landscape uncovers T cell trapping in human skin cancer

(2016) Reyk Hillert et al.   Scientific Reports

Multiplexed Epitope-Based Tissue Imaging for Discovery and Healthcare Applications

(2016) Bernd Bodenmiller   Cell Systems

Validation of biomarkers to predict response to immunotherapy in cancer: Volume I — pre-analytical and analytical validation

(2016) Giuseppe V. Masucci et al.   Journal for ImmunoTherapy of Cancer

Immune Checkpoint Targeting in Cancer Therapy: Toward Combination Strategies with Curative Potential

(2015) Padmanee Sharma et al.   CELL

Nivolumab versus chemotherapy in patients with advanced melanoma who progressed after anti-CTLA-4 treatment (CheckMate 037): a randomised, controlled, open-label, phase 3 trial

(2015) Jeffrey S Weber et al.   LANCET ONCOLOGY

The European antibody network's practical guide to finding and validating suitable antibodies for research

(2015) Giovanna Roncador et al.   mAbs

Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues

(2015) Je Hyuk Lee et al.   Nature Protocols

PD-1 Blockade with Nivolumab in Relapsed or Refractory Hodgkin's Lymphoma

(2015) Stephen M. Ansell et al.   NEW ENGLAND JOURNAL OF MEDICINE

Spatially resolved, highly multiplexed RNA profiling in single cells

(2015) K. H. Chen et al.   SCIENCE

Human and Mouse CD8+CD25+FOXP3+ Regulatory T Cells at Steady State and during Interleukin-2 Therapy

(2015) Guillaume Churlaud et al.   Frontiers in Immunology

A Single Slide Multiplex Assay for the Evaluation of Classical Hodgkin Lymphoma

(2014) Denise Hollman-Hewgley et al.   AMERICAN JOURNAL OF SURGICAL PATHOLOGY

Survival, Durable Tumor Remission, and Long-Term Safety in Patients With Advanced Melanoma Receiving Nivolumab

(2014) Suzanne L. Topalian et al.   JOURNAL OF CLINICAL ONCOLOGY

Quantitative measurement of cancer tissue biomarkers in the lab and in the clinic

(2014) Daniel E Carvajal-Hausdorf et al.   LABORATORY INVESTIGATION

Preanalytical variables and phosphoepitope expression in FFPE tissue: quantitative epitope assessment after variable cold ischemic time

(2014) Maria Vassilakopoulou et al.   LABORATORY INVESTIGATION

Multiplexed ion beam imaging of human breast tumors

(2014) Michael Angelo et al.   NATURE MEDICINE

Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry

(2014) Charlotte Giesen et al.   NATURE METHODS

Highly Multiplexed Subcellular RNA Sequencing in Situ

(2014) J. H. Lee et al.   SCIENCE

Methodological requirements for valid tissue-based biomarker studies that can be used in clinical practice

(2014) Lawrence D. True   VIRCHOWS ARCHIV

Metabolic Pathways in Immune Cell Activation and Quiescence

(2013) Erika L. Pearce et al.   IMMUNITY

viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia

(2013) El-ad David Amir et al.   NATURE BIOTECHNOLOGY

In situ sequencing for RNA analysis in preserved tissue and cells

(2013) Rongqin Ke et al.   NATURE METHODS

Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue

(2013) M. J. Gerdes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-content multiplexed tissue imaging and quantification for cancer drug discovery

(2012) Beverly L. Falcon et al.   DRUG DISCOVERY TODAY

Safety, Activity, and Immune Correlates of Anti–PD-1 Antibody in Cancer

(2012) Suzanne L. Topalian et al.   NEW ENGLAND JOURNAL OF MEDICINE

Study Designs and Statistical Analyses for Biomarker Research

(2012) Masahiko Gosho et al.   SENSORS

The challenge and importance of standardizing pre-analytical variables in surgical pathology specimens for clinical care and translational research

(2011) DG Hicks et al.   BIOTECHNIC & HISTOCHEMISTRY

Phenotype, effector function, and tissue localization of PD-1-expressing human follicular helper T cell subsets

(2011) Chuanwu Wang et al.   BMC IMMUNOLOGY

Immune Inhibitory Molecules LAG-3 and PD-1 Synergistically Regulate T-cell Function to Promote Tumoral Immune Escape

(2011) S.-R. Woo et al.   CANCER RESEARCH

Method of calibration of a fluorescence microscope for quantitative studies

(2011) KATARZYNA M. KEDZIORA et al.   JOURNAL OF MICROSCOPY

Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum

(2011) S. C. Bendall et al.   SCIENCE

Antibody validation

(2010) Jennifer Bordeaux et al.   BIOTECHNIQUES

Predictive biomarker validation in practice: lessons from real trials

(2010) Sumithra J Mandrekar et al.   Clinical Trials

Increased liver-infiltrating CD8+FoxP3+ regulatory T cells are associated with tumor stage in hepatocellular carcinoma patients

(2010) Zhi-Qing Yang et al.   HUMAN IMMUNOLOGY

In situ detection and genotyping of individual mRNA molecules

(2010) Chatarina Larsson et al.   NATURE METHODS

Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry

(2010) Erik Fung et al.   Nature Protocols

Tumor-infiltrating NY-ESO-1–specific CD8+T cells are negatively regulated by LAG-3 and PD-1 in human ovarian cancer

(2010) Junko Matsuzaki et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antibody-Based Protein Multiplex Platforms: Technical and Operational Challenges

(2009) A. A. Ellington et al.   CLINICAL CHEMISTRY

Accuracy and precision in quantitative fluorescence microscopy

(2009) Jennifer C. Waters   JOURNAL OF CELL BIOLOGY

Simple: A Sequential Immunoperoxidase Labeling and Erasing Method

(2009) George Glass et al.   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

Antibody Elution Method for Multiple Immunohistochemistry on Primary Antibodies Raised in the Same Species and of the Same Subtype

(2009) Daniel Pirici et al.   JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY

Use of Archived Specimens in Evaluation of Prognostic and Predictive Biomarkers

(2009) R. M. Simon et al.   JNCI-Journal of the National Cancer Institute

Tissue is alive: New technologies are needed to address the problems of protein biomarker pre-analytical variability

(2009) Virginia Espina et al.   Proteomics Clinical Applications