Article
Engineering, Electrical & Electronic
Baini Li, Tianran Wang, Weiguo Bai, Qingqing Su, Xuezhong Wu, Peitao Dong
Summary: A label-free detection scheme based on surface-enhanced Raman Scattering (SERS) was proposed for ultra-sensitive on-site rapid detection of anthrax protective antigens (PA). The detection limit of PA in human serum albumin (HSA) solution was found to be 100 μg/mL, lower than the PA concentration in human serum after anthrax infection. Principal Component Analysis (PCA) method was used to classify the SERS data of PA, HSA, and the mixture into three completely separated areas. The entire testing process only takes about eight minutes, faster than traditional technologies like Enzyme-linked Immunosorbent Assay (ELISA).
IEEE SENSORS JOURNAL
(2021)
Article
Nanoscience & Nanotechnology
Eun Hye Koh, Won-Chul Lee, Yeong-Jin Choi, Joung-Il Moon, Jinah Jang, Sung-Gyu Park, Jaebum Choo, Dong-Ho Kim, Ho Sang Jung
Summary: A wearable SERS sensor has been developed for molecular sweat sensing, with the capability of measuring Raman signals without detachment from the skin. Through a multi-layer design, the sensor can absorb sweat, protect the skin, enhance signal, and has great potential for drug detection applications.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Spectroscopy
Xinpeng Lv, Zhe Zhang, Yue Zhao, Xiaomeng Sun, Heng Jiang, Shuwen Zhang, Xianqi Sun, Xiaohong Qiu, Yang Li
Summary: This study develops a suitable virus detection hotspot using surface-enhanced Raman scattering (SERS) by reducing sodium borohydride and aggregating silver nanoparticles. Monkeypox virus and human papillomavirus fingerprints were successfully obtained and identified in serum and artificial vaginal discharge, respectively, using the principal component analysis method. The method has a low detection limit of 100 copies/mL, good reproducibility, and signal-to-noise ratio, and can solve the SERS signal interference problem in complex biological samples, showing potential for clinical application.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Physical
C. K. Chung, C. Y. Yu
Summary: A new type of SERS substrate is proposed, which does not require the use of metal nanoparticles. The substrate is fabricated by electrochemically micromachining aluminum foil to create a roughened surface with randomly-distributed 3D over-etched cavities and nanopores of anodized aluminum oxide (AAO). This SERS substrate demonstrates high analytical enhancement and low detection limit, making it suitable for rapid detection of substances such as methylene blue.
APPLIED SURFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Ping Zhang, Xi-Hao Wu, Lan Su, Hui-Qin Wang, Tai-Feng Lin, Ya-Ping Fang, Hui-Min Zhao, Wen-Jing Lu, Meng-Jia Liu, Wen-Bo Liu, Da-Wei Zheng
Summary: This study rapidly recorded cefotaxime (CTX)-derived resistance in Salmonella typhimurium using a portable Raman spectrometer. The molecular changes in drug-resistant strains were monitored using label-free surface-enhanced Raman scattering (SERS). Different degrees of resistance could be accurately discriminated through statistical analysis of bacterial SERS profiles. The results of this preliminary study demonstrate the potential of SERS as a supplementary method in antibiotic resistance testing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Environmental
Guangda Xu, Na Li, Ye Sun, Ce Gao, Liping Ma, Peng Song, Lixin Xia
Summary: A label-free, concise, time-efficient, ultrasensitive, and selective strategy for the determination of Fe2+ ion based on SERRS spectroscopy has been developed. The platform displayed a wide linear range with an unprecedented detection limit, offering a new universal route for practical detection in various water samples. The formation of [Fe(Bpy)(3)](2+) complexes with Fe2+ induced the aggregation of AgNPs and produced an intense Raman signal, providing a theoretical foundation for quantitative analysis of Fe2+.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Analytical
Yuan Zhang, De-Sheng Zhan, Xiao-Ying Xu, Zhe Zhang, Mahmoud Elsayed Hafez, Yue He, Yang Li, Da-Wei Li
Summary: This study developed a SERS platform for detecting DNA methylation by modifying silver nanoparticles with zirconium ions. The use of zirconium ions enabled the opening of folded DNA molecules, allowing for the identification of subtle differences between normal and methylated DNA with single base-level sensitivity.
Article
Chemistry, Multidisciplinary
Shi Xuan Leong, Charlynn Sher Lin Koh, Howard Yi Fan Sim, Yih Hong Lee, Xuemei Han, Gia Chuong Phan-Quang, Xing Yi Ling
Summary: The study successfully achieves direct, label-free SERS sensing of biologically important enantiomers by synergizing asymmetric nanoporous gold (NPG) nanoparticles with electrochemical-SERS. This strategy is versatile and can be readily extended to detect various enantiomers, and allows for multiplex quantification of enantiomeric ratios with excellent predictive performance. The research offers a paradigm shift in achieving label-free chiral SERS sensing of various enantiomers.
Article
Biochemical Research Methods
Chang-Chun Xiong, Shan-Shan Zhu, Deng-Hui Yan, Yu-Dong Yao, Zhe Zhang, Guo-Jun Zhang, Shuo Chen
Summary: Early, express, and reliable detection of cancer using surface-enhanced Raman scattering (SERS) combined with deep learning can provide a rapid, reliable, and non-invasive approach. This study collected serum samples from healthy controls and cancer patients and used 1D-CNN for precise identification, achieving high accuracy of 98.27%. The contributions of SERS peaks indicated potential biomarkers for different types of cancer, providing insights into the intelligent diagnosis of cancers based on label-free serum SERS. The integration of label-free SERS and deep learning has great potential to significantly improve precise diagnosis in clinical practice.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zhe Zhang, Shen Jiang, Xiaotong Wang, Tuo Dong, Yunpeng Wang, Dan Li, Xin Gao, Zhangyi Qu, Yang Li
Summary: This study presents a novel enhanced substrate for label-free detection of respiratory viruses using surface-enhanced Raman Scattering. The method involves the use of sodium borohydride to reduce silver ions, eliminating the disorganized peak signal of traditional reducing agents. The study successfully obtained the fingerprints and concentration-dependent curves of various respiratory viruses, including SARS-CoV-2, and identified them in serum and saliva within two minutes using linear discriminant diagnostic analysis. This enhanced substrate is therefore highly valuable for the global response to the COVID-19 pandemic.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Biochemical Research Methods
Nan Lu, Hui Min Tay, Chayakorn Petchakup, Linwei He, Lingyan Gong, Kay Khine Maw, Sheng Yuan Leong, Wan Wei Lok, Hong Boon Ong, Ruya Guo, King Ho Holden Li, Han Wei Hou
Summary: Blood tests are standard clinical procedures for disease and health condition screening, but the complex cellular background and biomolecular composition often make accurate analysis challenging. Label-free microfluidic technologies that rely on intrinsic cell properties are emerging as a promising approach for point-of-care blood diagnostics. This review summarizes recent advances in label-free blood cell separation, single cell analysis, and the combination of microfluidics with machine learning for enhanced clinical studies.
Article
Chemistry, Applied
Md Mehedi Hassan, Peihuan He, Muhammad Zareef, Huanhuan Li, Quansheng Chen
Summary: A hollow Au/Ag nano-flower nanosensor was synthesized for label-free and ultrasensitive detection of chloramphenicol, integrating surface-enhanced Raman scattering and multivariate calibration. The CP generated a strong SERS signal when adsorbed on the surface of HAu/Ag NFs, showing excellent linearity with CARS-PLS in the range of 0.0001-1000 μg/mL. The CARS-PLS method had the lowest prediction errors for milk and water samples, demonstrating high accuracy and precision of the proposed method.
Article
Chemistry, Analytical
Xin Liu, Alei Dang, Tiehu Li, Yiting Sun, Weibin Deng, Tung-Chun Lee, Yong Yang, Amir Zada, Boning Wang, Yuhui Liu, Shaoheng Wu, Tingkai Zhao
Summary: This article introduces a surface-enhanced Raman scattering (SERS) sensor that achieves reliable and sensitive large-scale rapid screening of target analytes. The sensor demonstrates ultralow limit of detection, outstanding sensitivity, and high stability. It also allows for the multiplexed detection of pesticide residues and the recognition of target molecules in a wide range of fields.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Guangda Xu, Qijia Zhang, Ce Gao, Liping Ma, Peng Song, Lixin Xia
Summary: A surface-enhanced Raman scattering (SERS) sensor utilizing phenylacetylene as a label-free probe has been established for the detection of mercury ions. The sensor shows high sensitivity, excellent selectivity, reproducibility, and accuracy in analyzing mercury ions in lake water samples. The results suggest that the SERS platform is a promising alternative tool for practical determination of mercury ions.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Xiaoming Su, Xinyu Liu, Yangcenzi Xie, Mingyang Chen, Hong Zhong, Ming Li
Summary: The widespread abuse of fentanyl has become a major global public health concern, leading to a drastic increase in overdose deaths. A recently developed freestanding surface-enhanced Raman spectroscopy (SERS) biosensor, called the FrEnSERS biosensor, has shown excellent capability in identifying and quantifying trace amounts of fentanyl in biofluids. This biosensor, composed of a reduced graphene oxide membrane decorated with hydrophobic Au nanostars, combines high SERS enhancement and analyte enrichment to achieve remarkable SERS performance. It has demonstrated sensitive and quantitative detection of fentanyl in serum and urine, with a wide dynamic range and low limits of detection. The FrEnSERS biosensor is a promising tool for forensic analysis and clinical diagnosis, providing a sensitive, cost-effective, and reliable method for rapid quantitative analysis of fentanyl in biofluids.
ANALYTICAL CHEMISTRY
(2023)
