Article
Endocrinology & Metabolism
Marija K. Simic, Sindhu T. Mohanty, Ya Xiao, Tegan L. Cheng, Victoria E. Taylor, Olga Charlat, Peter I. Croucher, Michelle M. McDonald
Summary: In this study, a novel anti-LRP6 antibody that enhances Wnt signaling and a Wnt antagonist DKK1 were used in combination to prevent bone loss and improve bone structure in multiple myeloma. The combination strategy showed significant improvements in bone volume and resistance to fracture by inhibiting bone resorption without exacerbating tumor activity.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Endocrinology & Metabolism
Roy B. Choi, April M. Hoggatt, Daniel J. Horan, Emily Z. Rogers, Gabriela G. Loots, Alexander G. Robling
Summary: The development of Wnt-based osteoanabolic agents has progressed rapidly in recent years, showing potent effects on bone homeostasis. Simultaneous inhibition of sclerostin and Dkk1 can enhance the effects in the cancellous bone. Further research reveals that Sostdc1 can be co-inhibited with sclerostin to improve cortical bone properties. Combined administration of sclerostin antibody and Sostdc1 antibody produces potentiation of cortical bone gain.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Stefano Negri, Yiyun Wang, Takashi Sono, Qizhi Qin, Ginny Ching-Yun Hsu, Masnsen Cherief, Jiajia Xu, Seungyong Lee, Robert J. Tower, Victoria Yu, Abhi Piplani, Carolyn A. Meyers, Kristen Broderick, Min Lee, Aaron W. James
Summary: Research has shown that anti-DKK1 neutralizing antibodies can enhance the osteogenic differentiation of human adipose-derived stem cells, aiding in bone repair, and demonstrating potential beneficial effects in a mouse model.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Review
Pharmacology & Pharmacy
Hewen Jiang, Zongkang Zhang, Yuanyuan Yu, Hang Yin Chu, Sifan Yu, Shanshan Yao, Ge Zhang, Bao-Ting Zhang
Summary: This article reviews the currently available or under study DKK1 inhibitors and discusses future research prospects.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Endocrinology & Metabolism
Zhao Li, Xin Xing, Mario Armando Gomez-Salazar, Mingxin Xu, Stefano Negri, Jiajia Xu, Aaron W. James
Summary: Targeting the inhibitor WAY-262611 promotes local bone formation and has potential clinical value for osteoporotic bone repair. This small molecule enhances osteogenesis and Wnt signaling activity in bone marrow-derived stem/stromal cells, leading to improved lumbar fusion in an ovariectomized rat model.
Article
Biology
Zhen Yang, Xinping Huang, Jiaye Zhang, Kai You, Yue Xiong, Ji Fang, Anteneh Getachew, Ziqi Cheng, Xiaorui Yu, Yan Wang, Feima Wu, Ning Wang, Shufen Feng, Xianhua Lin, Fan Yang, Yan Chen, Hongcheng Wei, Yin-xiong Li
Summary: Nonalcoholic fatty liver disease (NAFLD) is a prevalent global disease with about 25% of patients progressing to severe conditions. Up-regulated DKK1 plays a pivotal role in the development and progression of NAFLD induced by high-fat diet, making it a potential therapeutic and diagnostic target.
LIFE SCIENCE ALLIANCE
(2022)
Article
Biochemistry & Molecular Biology
Lisa Y. Lawson, Nicole Migotsky, Christopher J. Chermside-Scabbo, John T. Shuster, Kyu Sang Joeng, Roberto Civitelli, Brendan Lee, Matthew J. Silva
Summary: Mechanical loading stimulates bone formation, and the Wnt signaling pathway plays a crucial role in the skeletal response to loading. Specifically, Wnt1 mediates the mechano-adaptive response to loading.
Review
Cell Biology
Dimitri Papukashvili, Nino Rcheulishvili, Cong Liu, Fengfei Xie, Deependra Tyagi, Yunjiao He, Peng George Wang
Summary: Androgenetic alopecia (AGA) is a challenging issue that requires the development of new treatment methods. The Wnt/β-catenin signaling pathway is crucial for hair growth and can be targeted for AGA therapy.
Review
Biochemistry & Molecular Biology
Ahmed Omran, Diana Atanasova, Filip Landgren, Per Magnusson
Summary: This article reviews recent progress in the study of SOST gene, including the regulation pathways, structural and functional properties of sclerostin, and its pathophysiology. It also introduces the development of new assay methods and the importance of standardization work.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Emily G. Atkinson, Michele Adaway, Daniel J. Horan, Crystal Korff, Angela Klunk, Ashley L. Orr, Katherine Ratz, Teresita Bellido, Lilian I. Plotkin, Alexander G. Robling, Joseph P. Bidwell
Summary: Activation of bone anabolic pathways is a promising strategy for treating severe osteoporosis. The inactivation of the Nmp4 gene enhances the bone anabolic effects of parathyroid hormone (PTH). The Prx1-expressing mesenchymal stem/progenitor cells (MSPCs) play a crucial role in the efficacy of PTH therapy.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Dimitri Papukashvili, Cong Liu, Nino Rcheulishvili, Fengfei Xie, Xingyun Wang, Shunping Feng, Xiu Sun, Chi Zhang, Yingyu Li, Yunjiao He, Peng George Wang
Summary: Despite advancements in medical research, androgenetic alopecia (AGA) still lacks an ideal treatment. This study suggests that targeting dickkopf-related protein 1 (DKK1) with small interfering RNA (siRNA) may promote hair growth regulation in the AGA population by activating the Wnt/I3-catenin signaling pathway. The results showed that DKK1-targeting siRNA had comparable efficacy to the FDA-approved minoxidil topical solution.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Nahyun Choi, Juyeong Hwang, Doo Yeong Kim, Jino Kim, Seung Yong Song, Jong-Hyuk Sung
Summary: This study discovered that the secretion of DKK1 by adipose-derived stem cells (ASCs) plays a crucial role in the development and progression of alopecia areata (AA). Knocking out DKK1 in ASCs enhances cell motility, reduces cellular senescence, and decreases the expression of inflammatory cytokines. Cell therapy using DKK1-KO-ASCs shows greater efficiency compared to using naive ASCs.
CELL PROLIFERATION
(2023)
Article
Cell Biology
Lina Qi, Jiani Chen, Biting Zhou, Kailun Xu, Kailai Wang, Zhihao Fang, Yingkuan Shao, Ying Yuan, Shu Zheng, Wangxiong Hu
Summary: HOXC6 is overexpressed in right-sided colon cancer and promotes migration and invasion of colon cancer cells by activating the Wnt/beta-catenin signaling pathway. Silencing of HOXC6 can make colon cancer cells more sensitive to irinotecan.
CELL DEATH & DISEASE
(2021)
Article
Medicine, Research & Experimental
Yujia Zheng, Zheng Zhou, Ran Wei, Chu Xiao, Hao Zhang, Tao Fan, Bo Zheng, Chunxiang Li, Jie He
Summary: The study reveals that PCBP1 inhibits the development of lung adenocarcinoma (LUAD) by upregulating DKK1 to inactivate the Wnt/beta-catenin pathway.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Medicine, Research & Experimental
Peng Zeng, Jie Yang, Lipei Liu, Xiaoxiao Yang, Zhi Yao, Chuanrui Ma, Haibo Zhu, Jiamin Su, Qian Zhao, Ke Feng, Shu Yang, Yan Zhu, Xiaoju Li, Wenguang Wang, Yajun Duan, Jihong Han, Yuanli Chen
Summary: The study showed a positive correlation between calcification in human aortic valves and ERK1/2 activity. Inhibition of ERK1/2 reduced atherosclerosis and calcification in animal models, as well as calcification in SMCs and arterial rings. The reduction of calcification was due to decreased expression of ALP, BMP2, and RUNX2, and activation of DKK1 and LRP6 expression, thereby inactivating Wnt signaling pathways in SMCs.
Article
Endocrinology & Metabolism
Ryan C. Chai, Michelle M. McDonald
Summary: This review highlights recent findings achieved through intravital imaging, emphasizing the importance of understanding the pathways and cellular interactions that regulate tumour cell seeding, dormancy, and growth in bone. Advances in imaging techniques have provided insights into the behavior of bone marrow resident cells and the elements of the bone microenvironment that tumour cells interact with. Combining imaging insights with single cell RNA sequencing data opens up new avenues for research on the cell-cell interactions controlling tumour cell growth in bone.
Article
Chemistry, Medicinal
Jiadai Wu, Helen Power, Monica Miranda-Saksena, Peter Valtchev, Aaron Schindeler, Anthony L. Cunningham, Fariba Dehghani
Summary: This study identified potential antiviral compounds using computational methods and experimental evaluations, with one active compound showing a novel antiviral mechanism of action.
Article
Orthopedics
Alexandra K. O'Donohue, Aiken Dao, Justin D. Bobyn, Craig F. Munns, David G. Little, Aaron Schindeler
Summary: Osteogenesis imperfecta (OI), a genetic bone fragility disorder, can be treated with bone morphogenetic proteins (BMPs) and bisphosphonates (BPs). However, the use of BMPs as local agents for fracture healing in OI may be less effective, while the combination of BMP-2 and BPs shows considerable improvements in bone volume and polar moment of inertia. Therefore, caution should be taken when using BMP-2 alone in OI surgical settings, but the off-label administration of BPs may help overcome this limitation.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Endocrinology & Metabolism
Amanda M. Rooney, Tyler J. McNeill, F. Patrick Ross, Mathias P. G. Bostrom, Marjolein C. H. van der Meulen
Summary: Parathyroid hormone (PTH) is an effective treatment for increasing bone mass and reducing fracture risk in osteoporosis. Its effects vary depending on the site, with greater bone mass increase at the spine than the hip and no increase at the radius. The differences in local loading mode may contribute to these variations. This study found that compression loading is more anabolic than tension loading, and PTH enhances the effect of loading, particularly under compression.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Endocrinology & Metabolism
Carolyn Chlebek, Jacob A. Moore, F. Patrick Ross, Marjolein C. H. van der Meulen
Summary: Osteoporosis affects over 200 million women worldwide, with one-third of them predicted to experience osteoporotic fractures in their lifetime. A study using a mouse model found that mechanical loading can stimulate bone formation, potentially identifying new pathways for oral treatment.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Endocrinology & Metabolism
Alexandra K. O'Donohue, Ya Xiao, Lucinda R. Lee, Timothy Schofield, Tegan L. Cheng, Craig F. Munns, Paul A. Baldock, Aaron Schindeler
Summary: In this study, a bone-targeted recombinant adeno-associated virus (AAV) vector was used to efficiently produce postnatal skeletal knockout mice using gene-floxed strains. The AAV8-Sp7-Cre vector induced widespread and persistent recombination in the bone compartment, resulting in significant increases in bone volume and strength. This research confirms the important postnatal role of the Sost gene in regulating bone homeostasis and identifies a novel role in canalicular remodeling.
Article
Endocrinology & Metabolism
Marija K. Simic, Sindhu T. Mohanty, Ya Xiao, Tegan L. Cheng, Victoria E. Taylor, Olga Charlat, Peter I. Croucher, Michelle M. McDonald
Summary: In this study, a novel anti-LRP6 antibody that enhances Wnt signaling and a Wnt antagonist DKK1 were used in combination to prevent bone loss and improve bone structure in multiple myeloma. The combination strategy showed significant improvements in bone volume and resistance to fracture by inhibiting bone resorption without exacerbating tumor activity.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Oncology
Bridie S. Mulholland, Pierre Hofstee, Ewan K. A. Millar, Dana Bliuc, Sandra O'Toole, Mark R. Forwood, Michelle M. McDonald
Summary: This study found an association between MCP-1 expression in the primary tumor and distant relapse and risk of death in breast cancer patients, particularly in ER- breast cancers. However, this association was likely a result of most low MCP-1-expressing ER- breast cancers being in advanced stages. The study emphasized the need for further investigation into the role of MCP-1 in breast cancer progression and improved characterization of MCP-1 in breast cancers.
Article
Endocrinology & Metabolism
Terrence H. Diamond, Carl Bryant, Richard Quinn, Sindhu T. Mohanty, Fiona Bonar, Paul A. Baldock, Michelle M. McDonald
Summary: A 71-year-old man with high bone mass and diffuse osteosclerosis presented with hypocalcemia and hypocalciuria. PET confirmed no focal areas of abnormal uptake, suggesting no underlying primary malignancy or metastatic disease. Bone biopsy showed sclerotic woven and lamellar bone with tumor cell infiltration. The unique bone disorder raises the possibility of a novel anabolic paracrine factor secreted by an adenocarcinoma of unknown primary. Differential diagnosis and potential mechanisms for the high bone mass are discussed.
Article
Infectious Diseases
Aiken Dao, Alexandra K. O'Donohue, Emily R. Vasiljevski, Justin D. Bobyn, David G. Little, Aaron Schindeler
Summary: This study developed a reliable and cost-effective murine bone infection model that mimics bacterial bone infections associated with biofilm and metal implants. The tibial drilled hole (TDH) and needle insertion surgery (NIS) infection models were compared, and both achieved a 100% infection rate when a metal implant was present. The NIS model was simpler to perform than the TDH model.
JOURNAL OF BONE AND JOINT INFECTION
(2023)
Review
Cell Biology
Helen Power, Peter Valtchev, Fariba Dehghani, Aaron Schindeler
Summary: This article discusses the classification of senolytic drugs and methods for screening new drugs. Researchers have discovered a range of senolytic drugs and introduce their different categories and mechanisms of action. The article also highlights the need for further research into drug targets and mechanisms, as well as rigorous evaluation in pre-clinical models and human trials.
Article
Cell Biology
Sheela R. Damle, Agata K. Krzyzanowska, Maximilian K. Korsun, Kyle W. Morse, Susannah Gilbert, Han Jo Kim, Oheneba Boachie-Adjei, Bernard A. Rawlins, Marjolein C. H. van der Meulen, Matthew B. Greenblatt, Chisa Hidaka, Matthew E. Cunningham
Summary: This study investigated the possibility of inducing bone formation and angiogenesis in the intervertebral discs (IVDs) by manipulating the IVD environment. The results showed that gene delivery and proteoglycanase treatment can promote bone and vascular growth in the IVD. However, proteoglycanase treatment impaired bone formation and reduced histological staining in the IVD.
Article
Rheumatology
Sohel M. Julovi, Aiken Dao, Katie Trinh, Alexandra K. O'Donohue, Cindy Shu, Susan Smith, Meena Shingde, Aaron Schindeler, Natasha M. Rogers, Christopher B. Little
Summary: This study developed a preclinical comorbid model of chronic kidney disease (CKD) and osteoarthritis (OA), and investigated the disease-modifying interactions between these two diseases. The findings demonstrated bidirectional disease-modifying interaction between CKD and OA.
Article
Endocrinology & Metabolism
Kyle D. Anderson, Christian Beckmann, Saskia Heermant, Frank C. Ko, Bryan Dulion, Imad Tarhoni, Jeffrey A. Borgia, Amarjit S. Virdi, Markus A. Wimmer, D. Rick Sumner, Ryan D. Ross
Summary: An increasing number of patients with type 2 diabetes will require total joint replacement in the future, and this study investigates the effects and mechanisms of implant placement in diabetic rats. The results suggest that peri-implant structure and matrix composition play a role in implant fixation, and postoperative inflammation may contribute to poor outcomes in type 2 diabetes patients undergoing joint replacement surgeries.
Article
Endocrinology & Metabolism
V. A. Shahen, A. Schindeler, M. S. Rybchyn, C. M. Girgis, B. Mulholland, R. S. Mason, I. Levinger, T. C. Brennan-Speranza
Summary: Patients with type 2 diabetes mellitus have a higher risk of fractures despite normal to high bone mineral density. This study investigated the effects of high glucose levels on human osteoblasts and tested the efficacy of parathyroid hormone and cinacalcet in promoting cell growth and matrix mineralization. The findings suggest that combination therapy with these two agents may ameliorate the negative impact of chronic high blood glucose on bone remodeling.
CALCIFIED TISSUE INTERNATIONAL
(2023)
Review
Endocrinology & Metabolism
Jemima E. Schadow, David Maxey, Toby O. Smith, Mikko A. J. Finnila, Sarah L. Manske, Neil A. Segal, Andy Kin On Wong, Rachel A. Davey, Tom Turmezei, Kathryn S. Stok
Summary: This study systematically reviewed the published parameters for assessing subchondral bone in human osteoarthritis using computed tomography. The study identified clinically meaningful parameter categories and emphasized the importance of quantification and standardized measurement methods for improving the evaluation of disease progression.
Article
Endocrinology & Metabolism
Lindsay L. Loundagin, Kim D. Harrison, Xuan Wei, David M. L. Cooper
Summary: This study developed new techniques to define zones of BMU activity based on the 3D morphology of remodeling spaces in rabbit cortical bone and integrated morphological data with the BMU longitudinal erosion rate (LER) to elucidate the spatial-temporal coordination of BMUs and estimate mineral apposition rate (MAR). The results showed that the manual and semi-automated methods accurately defined the zones of remodeling spaces, and these techniques have the potential to assess dynamic parameters of bone resorption and formation.
Article
Endocrinology & Metabolism
Soroush Masrouri, Farzad Esmaeili, Maryam Tohidi, Fereidoun Azizi, Farzad Hadaegh
Summary: This study examined the association between estimated glomerular filtration rate (eGFR) decline and fracture incidence. The results showed that rapid kidney function decline (RKFD) can increase the incidence of fractures among the general population.
Article
Endocrinology & Metabolism
Steven J. Meas, Gabriella M. Daire, Michael A. Friedman, Rachel Denapoli, Preetam Ghosh, Joshua N. Farr, Henry J. Donahue
Summary: Age- and disuse-related bone loss both lead to decreases in bone mineral density, cortical thickness, and trabecular thickness and connectivity. It is important to experimentally compare these two mechanisms at a structural and transcriptomic level to better understand their similarities and differences. This study compares the effects of hindlimb unloading and aging on bone microarchitecture and gene expression in mice, finding that while both induce similar changes, aging has a greater impact on the transcriptome and tissue level.
Correction
Endocrinology & Metabolism
Masaru Matsuoka, Sho Tsukamoto, Yuta Orihara, Rieko Kawamura, Mai Kuratani, Nobuhiko Haga, Kenji Ikebuchi, Takenobu Katagiri
Article
Endocrinology & Metabolism
Rachel Kohler, Amy Creecy, David R. Williams, Matthew R. Allen, Joseph M. Wallace
Summary: Osteogenesis imperfecta is a hereditary bone disease that weakens bones and increase fracture risk. Current interventions mainly focus on increasing bone mass, but the compromised tissue-level material properties are not addressed. A study found that a RAL analog could reduce fracture risk, but further development is needed for optimal results in patients with osteogenesis imperfecta.
Article
Endocrinology & Metabolism
So Jeong Park, Eunhye Ji, Hyun Ju Yoo, Kyunggon Kim, Sunghwan Ji, Ji Yeon Baek, Jin Young Lee, Hee-Won Jung, Il-Young Jang, Eunju Lee, Namki Hong, Beom-Jun Kim
Summary: The study analyzed the relationship between serum lumican levels and osteosarcopenia in older adults, showing that older adults with osteosarcopenia had lower serum lumican levels. Lower serum lumican levels were associated with reduced bone mass and grip strength, indicating that lumican levels could be used as a biomarker for assessing the risk of osteosarcopenia, osteoporosis, or sarcopenia in older adults.
Article
Endocrinology & Metabolism
Michael B. Chavez, Michelle H. Tan, Tamara N. Kolli, Natalie L. Andras, Brian L. Foster
Summary: This study revealed the complex mechanisms by which disabling BSP functional domains led to profound and distinct changes in cementoblast cell functions, including dysregulated gene expression and reduced mineralization.
Article
Endocrinology & Metabolism
Julien Seiller, Blandine Merle, Romain Fort, Emilie Virot, Solene Poutrel, Giovanna Cannas, Arnaud Hot, Roland Chapurlat
Summary: The purpose of this study was to assess the prevalence of bone fragility in sickle cell patients and to evaluate the potential risk factors and associated complications.
Article
Endocrinology & Metabolism
Chirantap Oza, Anuradha Khadilkar, Pranay Goel, Madhura Karguppikar, Nikhil Shah, Nikhil Lohiya, Shruti Mondkar, Prashant Patil, Hemchand Prasad, Ankita Maheshwari, Dipali Ladkat, Neha Kajale, Chidvilas More, Devarati Khurjekar, Vaman Khadilkar
Summary: This study revealed that BoneXpert (BX) can be used for accurate assessment of bone age and screening of bone health in Indian children and youth with type-1 diabetes (T1D). 51.5% of T1D subjects showed significantly decreased metacarpal index (MCI). Height, Tanner stage, and vitamin D concentrations were positively correlated with MCI, while HbA1c and disease duration were negatively correlated with MCI.
Article
Endocrinology & Metabolism
Mariam R. Farman, Catherine Rehder, Theodora Malli, Cheryl Rockman-Greenberg, Kathryn Dahir, Gabriel Angel Martos-Moreno, Agnes Linglart, Keiichi Ozono, Lothar Seefried, Guillermo del Angel, Gerald Webersinke, Francesca Barbazza, Lisa K. John, Sewmi M. A. Delana Mudiyanselage, Florian Hoegler, Erica Burner Nading, Erin Huggins, Eric T. Rush, Ahmed El-Gazzar, Priya S. Kishnani, Wolfgang Hoegler
Summary: The ALPL gene variant database serves as an archive for interpreting the clinical significance of ALPL gene variants, facilitating the reclassification of VUS and continuous updates. The project establishes an international expert consortium, providing a multidisciplinary collaboration framework to improve genetic counseling and medical decision-making for HPP patients.
Article
Endocrinology & Metabolism
Giovanni Adami, Davide Gatti, Maurizio Rossini, Alessandro Giollo, Matteo Gatti, Francesco Bertoldo, Eugenia Bertoldo, Amy S. Mudano, Kenneth G. Saag, Ombretta Viapiana, Angelo Fassio
Summary: Certain diseases requiring glucocorticoids are independently associated with an increased risk of fractures. Chronic obstructive pulmonary disease (COPD) and neurological diseases are associated with both vertebral and non-vertebral fracture risk, while rheumatoid arthritis (RA) and inflammatory bowel disease (IBD) are only associated with non-vertebral fractures.
Article
Endocrinology & Metabolism
Frank C. Ko, Rong Xie, Brandon Willis, Zoe G. Herdman, Bryan A. Dulion, Hoomin Lee, Chun-do Oh, Di Chen, D. Rick Sumner
Summary: Intramembranous bone regeneration is important in joint and tooth replacement, but its underlying mechanisms are not well understood. This study found that increased periostin gene expression preceded increases in osteogenic genes during bone regeneration. Using a genetic mouse model, the researchers discovered that cells transiently expressing periostin played a critical role in intramedullary intramembranous bone regeneration.
Article
Endocrinology & Metabolism
T. Savikangas, T. H. Suominen, M. Alen, T. Rantalainen, S. Sipila
Summary: Regular exercise, especially high-intensity physical activity, can help slow down age-related bone loss and prevent a decline in femoral neck bone mineral density.
Article
Endocrinology & Metabolism
Mishaela R. Rubin, Ruban Dhaliwal
Summary: The increased risk of fractures observed in adults with type 1 diabetes (T1D) cannot be solely explained by modest decreases in areal bone mineral density (BMD). Accumulation of advanced glycation endproducts (AGEs) in bone has been suggested as a possible cause for the increased bone fragility in diabetes. Although the evidence linking AGEs and fractures in individuals with T1D is limited, recent data show that AGEs, as measured by skin intrinsic fluorescence, are a risk factor for lower BMD in T1D. Further research is needed to determine if there is a causal relationship between fractures and AGEs in T1D. If confirmed, this could lead to interventions that can reduce AGE accumulation and ultimately reduce fractures in T1D patients.