Article
Biochemical Research Methods
Athina G. Nicolaou, Ioannis J. Stavrou, Artemis P. Louppis, Michalis S. Constantinou, Constantina Kapnissi-Christodoulou
Summary: A validated UPLC-MS/MS method was developed for the detection of THC and CBN in cerumen samples, with quantification possible in 75% of cases.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Zhibo Fu, Qiangqiang Jia, Hongyang Zhang, Lu Kang, Xuezhi Sun, Min Zhang, Yuerong Wang, Ping Hu
Summary: A new method for simultaneous quantification of eleven SCFAs by derivatization combined with SPME and GC-MS/MS was developed, showing satisfactory linearity, low LOD and LOQ, as well as good precision and recovery rates in validation.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Ethel D. Weld, Teresa L. Parsons, Ryann Gollings, Marybeth McCauley, Beatriz Grinsztejn, Raphael J. Landovitz, Mark A. Marzinke
Summary: We developed and validated a LC-MS/MS assay for quantifying CAB and RPV from DBS, and derived conversion factors to estimate plasma concentrations from spotted blood.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Applied
Cesar N. Pegoraro, Diego Nutter, Mario Thevenon, Cristina L. Ramirez
Summary: This study compared the variability in chemical profiles of three different varieties of Cannabis sativa extracts using two different extraction and evaporation methods. Results showed that the highest mass of final extract was obtained when extracting inflorescences using the soxhlet method. Heating and the presence of oxygen during solvent evaporation greatly altered the final profiles, emphasizing the importance of careful consideration and standardization in the preparation of medicinal oils.
NATURAL PRODUCT RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Adina Borbely, Lilla Petho, Ildiko Szabo, Mohammed Al-Majidi, Arnold Steckel, Tibor Nagy, Sandor Keki, Gergo Kallo, Eva Csosz, Gabor Mezo, Gitta Schlosser
Summary: The use of peptide-drug conjugates as targeted antitumor therapeutics has gained significant attention. Our study focused on optimizing fragmentation conditions to suppress dissociation of anthracycline drug and provide good sequence coverage in tandem mass spectrometry. We found that electron-based dissociation methods can inhibit peptide backbone fragmentation, while efficient HCD fragmentation is influenced by factors such as amino acid sequences and charge states. High energy HCD and MALDI-TOF/TOF with CID mode are recommended for unambiguous sequence assignment and differentiation of conjugate isomers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Applied
Maria Tiziana Lisanti, Justine Laboyrie, Stephanie Marchand-Marion, Gilles de Revel, Luigi Moio, Laurent Riquier, Celine Franc
Summary: A novel automated method has been developed for the quantitative determination of nine terpenoids contributing to the minty notes in red wine bouquet. The method shows good performance and is suitable for the quantification of the pool of minty terpenoids in fourteen red wines.
Article
Chemistry, Analytical
Bo Sun, Jiuyang Liu, Pei Cai, Jianhua Wu, Wei Liu, Hankun Hu, Liang Liu
Summary: In this study, a simple and accurate LC-MS/MS assay was developed to quantify trastuzumab in human serum using aptamers for sample purification. The method involved extraction of trastuzumab from serum samples, followed by reduction, alkylation, trypsin digestion, and quantification using LC-MS/MS. The assay provided a novel approach for accurate and quantitative monitoring of mAb-trastuzumab.
Article
Chemistry, Applied
Artemis P. Louppis, Michalis S. Constantinou, Michael G. Kontominas
Summary: The study aimed to develop a rapid, simple, and reliable method for identifying and quantifying ten natural antioxidants in prickly pears. The analytical method was validated and successfully applied to prickly pear samples from Cyprus, with all antioxidant compounds detected except for myricetin and luteolin.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Chemistry, Multidisciplinary
Julius Pavlov, Ramu Errabelli, Sihang Xu, Zhaoyu Zheng, David Douce, Steve Bajic, Athula B. Attygalle
Summary: The helium-plasma (HePI) ionization mass spectrometry method was utilized to monitor the formation of reaction products and consumption of reactants in real time. The application procedure included nucleophilic acyl substitution reaction and 1,4-nucleophilic addition process. The setup of HePI source on a mass spectrometer allows it to be used in organic and analytical chemistry laboratories for teaching and demonstrations. By sampling the headspace of the reaction vessel with a Venturi suction device, the experiments can be quickly set up and adapted for any electrospray equipped mass spectrometer.
JOURNAL OF CHEMICAL EDUCATION
(2023)
Article
Medical Laboratory Technology
Martijn van Faassen, Anna van der Veen, Sonja van Ockenburg, Helma de Jong, Elisabeth G. E. de Vries, Ido P. Kema
Summary: This study validated a LC-MS/MS method for quantifying 6-sulfatoxymelatonin in urine, showing a decrease in levels with age. Biological variation of 6-sulfatoxymelatonin excretion was high between subjects and lower within subjects.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2021)
Article
Chemistry, Analytical
Susan C. Scott, Nicole M. Anders, Morgan Scardina, Christine L. Hann, Michelle A. Rudek
Summary: Vistusertib, an orally bioavailable mTOR inhibitor under clinical trials, was quantitated using a novel LC-MS/MS method to explore exposure-response relationships accurately. The method allowed accurate quantitation of high-concentration samples through dilution, and maintained long-term frozen stability.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Miriam Stempfer, Vera Reinstadler, Anna Lang, Herbert Oberacher
Summary: The study found that recreational cannabis may be contaminated with pesticides, mycotoxins, and synthetic cannabinoids, posing a major threat to public health. A screening workflow using non-targeted LC MS/MS identified contaminants including synthetic cannabinoids, pesticide residues, and mycotoxins in seized cannabis samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Plant Sciences
Carni Lipson Feder, Oded Cohen, Anna Shapira, Itay Katzir, Reut Peer, Ohad Guberman, Shiri Procaccia, Paula Berman, Moshe Flaishman, David Meiri
Summary: The study found that fertilization significantly decreased the concentration of phytocannabinoids in Cannabis plants, while monoterpenoids exhibited similar trends to phytocannabinoids. Specific phytocannabinoids and terpenoids showed an uncommon increase in concentration after fertilization with certain male plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Pharmacology & Pharmacy
Sabrina Wohlfart, Michael Kilian, Philip Storck, Thomas Gutsmann, Klaus Brandenburg, Walter Mier
Summary: A novel method for precise quantification of Pep19-2.5 in biological samples has been developed in this study, which is crucial for the treatment of sepsis. The method utilizes mass spectrometry and labeled amino acid residues to reliably quantify the peptide content.
Article
Biochemical Research Methods
Xinrui Wang, Yehong Han, Jiankun Cao, Hongyuan Yan
Summary: A headspace solid-phase microextraction (HS-SPME) method combined with gas chromatography-tandem mass spectrometry was developed and validated for the analysis of polychlorinated naphthalenes (PCNs) in shrimp. The method utilized a graphene aerogel as the SPME fiber coating, which exhibited good performance in terms of sorption/desorption for PCNs. The optimized method showed excellent linearity, low limits of detection, and satisfactory recoveries. Compared with previous methods, this developed method is simpler, more sensitive, and environmentally friendly.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Anesthesiology
Joshua Aviram, Gil M. Lewitus, Yelena Vysotski, Paula Berman, Anna Shapira, Shiri Procaccia, David Meiri
Summary: Studies have shown that women are more susceptible to adverse effects from conventional drugs. This study aimed to investigate the differences of medical cannabis-related adverse effects between women and men in patients with chronic noncancer pain. The results showed that women reported more medical cannabis-related adverse effects, possibly due to both the inherent sex effect and the consumption of specific phytocannabinoid compositions.
Article
Biophysics
Yaniv Shlosberg, Nimrod Krupnik, Tunde N. Toth, Ben Eichenbaum, Matan M. Meirovich, David Meiri, Omer Yehezkeli, Gadi Schuster, Alvaro Israel, Noam Adir
Summary: The study demonstrates the potential of using intact live marine macroalgae in BPECs to produce high electrical currents. These currents are significantly greater than those reported for single-cell microorganisms, indicating a promising approach for low-cost solar energy conversion technologies.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Pharmacology & Pharmacy
Shiri Procaccia, Gil Moshe Lewitus, Carni Lipson Feder, Anna Shapira, Paula Berman, David Meiri
Summary: Medical cannabis and its major components THC and CBD are gaining popularity for various medical purposes, but studies regarding their efficacy are inconclusive due to the versatile nature of the cannabis plant and the interplay between its compounds and the endocannabinoid system.
FRONTIERS IN PHARMACOLOGY
(2022)
Correction
Oncology
Gil Bar-Sela, Idan Cohen, Salvatore Campisi-Pinto, Gil M. Lewitus, Lanuel Oz-Ari, Ayellet Jehassi, Avivit Peer, Ilit Turgeman, Olga Vernicova, Paula Berman, Mira Wollner, Mor Moskovitz, David Meiri
Article
Biochemistry & Molecular Biology
Zhanna Yekhtin, Iman Khuja, David Meiri, Reuven Or, Osnat Almogi-Hazan
Summary: Phytocannabinoids have immune regulatory properties that can modulate macrophage function through the endocannabinoid system. In this study, the effects of specific phytocannabinoids on macrophage activation were evaluated. The results showed that phytocannabinoids inhibited macrophage activation, reduced inflammation in a murine model of colitis, and had unique effects on cytokine composition. Understanding the relationship between cannabinoids and immunity is crucial for developing targeted treatment strategies.
Article
Pharmacology & Pharmacy
Orit Stolar, Ariela Hazan, Roni Enten Vissoker, Ibrahim Abu Kishk, Dana Barchel, Mirit Lezinger, Adi Dagan, Nir Treves, David Meiri, Matitiahu Berkovitch, Elkana Kohn, Eli Heyman
Summary: This study aimed to assess the safety of CBD-rich oil treatment in children and adolescents with ASD. The results of analyzing data from 59 participants showed that CBD-rich cannabis oil had a good safety profile and did not cause significant side effects in the short term. However, further long-term monitoring with a larger number of participants is needed.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Barliz Waissengrin, Yasmin Leshem, Marwa Taya, David Meiri, Ofer Merimsky, Sivan Shamai, Ido Wolf, Tami Rubinek
Summary: This study aimed to evaluate the effect of cannabis on the efficiency of immune checkpoint inhibitors (ICIs) in non-small cell lung cancer (NSCLC) patients. The results from both preclinical and clinical observations showed that cannabis does not reduce the effectiveness of ICIs. Although cannabis users were younger and more likely to have symptoms such as brain metastasis, there was no correlation between cannabis use and overall survival. These findings provide reassurance that cannabis does not have a deleterious effect on treatment outcomes in this clinical setting.
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Oncology
Kifah Blal, Elazar Besser, Shiri Procaccia, Ouri Schwob, Yaniv Lerenthal, Jawad Abu Tair, David Meiri, Ofra Benny
Summary: The survival rate of head and neck cancer has only improved slightly over the last 25 years, highlighting the need for new treatment options. This research found that decarboxylated CBD-rich extracts had the strongest anti-tumor effects on head and neck cancer cells, and combining CBD with CBC in a 2:1 ratio enhanced the effect. These findings can help in developing better treatment options for head and neck cancer patients.
Article
Psychology, Clinical
Sharon R. Sznitman, David Meiri, Ben H. Amit, Dennis Rosenberg, Talya Greene
Summary: This study examines the relationship between the use of Medical Cannabis (MC) and sleep disturbances in posttraumatic stress disorder (PTSD) patients. The results show that a shorter time gap between MC use and sleep onset is associated with a lower likelihood of experiencing nightmares throughout the night. However, it is not associated with nightly awakenings or waking up too early. Individuals who used MC products with higher CBD concentrations reported fewer early awakenings.
JOURNAL OF ANXIETY DISORDERS
(2022)
Letter
Oncology
Elazar Besser, Anat Gelfand, Gil Moshe Lewitus, Hila Novak-Kotzer, Shiri Procaccia, Paula Berman, Igal Louria-Hayon, Inbar Shreiber-Livne, Yishai Ofran, David Meiri
CANCER COMMUNICATIONS
(2023)
Article
Plant Sciences
Paula Berman, Luis Alejandro de Haro, Adam Jozwiak, Sayantan Panda, Zoe Pinkas, Younghui Dong, Jelena Cveticanin, Ranjit Barbole, Rotem Livne, Tali Scherf, Eyal Shimoni, Smadar Levin-Zaidman, Nili Dezorella, Ekaterina Petrovich-Kopitman, Sagit Meir, Ilana Rogachev, Prashant D. Sonawane, Asaph Aharoni
Summary: This study uncovers a pathway for cannabinoid production in Helichrysum umbraculigerum, a plant that is genetically distinct from Cannabis. This provides a potential source of new cannabinoids and tools for metabolic engineering.
Article
Pharmacology & Pharmacy
Almog Uziel, Looz Milay, Shiri Procaccia, Ronen Cohen, Amir Burstein, Liron Sulimani, Inbar Shreiber-Livne, Dan Lewitus, David Meiri
Summary: The article introduces a fast and uniform drying method for Cannabis inflorescences based on solid-state microwave, which can considerably reduce drying time and preserve the composition of secondary metabolites. A drying temperature of 50 degrees Celsius was found to be the most effective in maintaining the chemical profile of phytocannabinoids and terpenoids.
CANNABIS AND CANNABINOID RESEARCH
(2022)
Article
Immunology
Ioannis D. Dimitriou, David Meiri, Yulia Jitkova, Alisha R. Elford, Marianne Koritzinsky, Aaron D. Schimmer, Pamela S. Ohashi, Nahum Sonenberg, Robert Rottapel
Summary: In this study, the researchers found that 4E-BP1/2 proteins play a critical role in the proliferation of mouse CD8(+) T cells and the development of antiviral effector function. They also discovered that the translation of genes related to mitochondrial biogenesis is impaired in T cells derived from 4E-BP1/2-deficient mice.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Clinical Neurology
Joshua Aviram, Gil M. Lewitus, Yelena Vysotski, Mahmoud Abu Amna, Anton Ouryvaev, Shiri Procaccia, Idan Cohen, Anca Leibovici, Luiza Akria, Dimitry Goncharov, Neomi Mativ, Avia Kauffman, Ayelet Shai, Gil Bar-Sela, David Meiri
Summary: The use of medical cannabis to treat cancer-related symptoms is increasing. This study prospectively followed up on the effectiveness and safety of medical cannabis treatment in oncology patients. The results showed that most patients experienced significant improvement in symptoms during medical cannabis treatment, with common but non-serious adverse effects.
FRONTIERS IN PAIN RESEARCH
(2022)