Article
Chemistry, Analytical
Yanjing Yang, Tong Luo, Yao He, Zhiwei Deng, Jiacheng Li, Hui Liu, Jing Nie, De Wang, Jin Huang, Shian Zhong
Summary: This study develops a nanotheranostic platform for the theranostics of cancer. The platform shows high efficiency in multiplex mRNA imaging and combinational therapy.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Renjie Niu, Fei Gao, Dou Wang, Dan Zhu, Shao Su, Shufen Chen, Lihui YuWen, Chunhai Fan, Lianhui Wang, Jie Chao
Summary: A DNA origami-directed pattern recognition strategy is utilized to assemble AuNCs into shape-controllable PGNs, creating localized field enhancement in gaps to generate hot spots for stronger single-molecule SERS signals.
Article
Chemistry, Multidisciplinary
Aurimas Kopustas, Sarune Ivanovaite, Tomas Rakickas, Ernesta Pocevicitue, Juste Paksaite, Tautvydas Karvelis, Mindaugas Zaremba, Elena Manakova, Marijonas Tutkus
Summary: Over the past 20 years, single-molecule methods in combination with new nanotechnological platforms have become extremely important for biophysical studies, facilitating experimental design and faster data acquisition. Utilizing a nanotechnological platform called DNA Curtains, which involves a flow-stretch of immobilized DNA molecules, allowed for stable immobilization and orientation of DNA molecules using a protein template-directed assembly. This method improves immobilization stability and enables the study of protein-DNA interactions under more controllable conditions, demonstrating potential applications in nucleic acid-interacting protein binding assays.
Article
Multidisciplinary Sciences
Qiaoqiao Ruan, Patrick J. Macdonald, Kerry M. Swift, Sergey Y. Tetin
Summary: This study demonstrates that direct single-molecule imaging can fundamentally improve the sensitivity and specificity of medically relevant assays, enhancing the screening of blood donations and diagnostic testing. Compared to traditional microparticle-based assays, single-molecule imaging increases signal-to-noise ratio and improves discrimination against nonspecific effects. This approach lowers detection sensitivity to attomolar levels and enables a wider linear detection range, shorter incubation times, simpler assay protocols, and reduced reagent consumption.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Jinrun Dong, Yuxian Lu, Yang Xu, Fanfan Chen, Jinmei Yang, Yuang Chen, Jiandong Feng
Summary: Optical imaging of single-molecule electrochemical reactions in aqueous solution enables super-resolution electrochemiluminescence microscopy, providing high spatiotemporal resolution for monitoring adhesion dynamics of live cells. Single-molecule methods go beyond ensemble averages and reveal statistical distribution of reaction positions, pathways, and dynamics, advancing fundamental understanding of electrochemical reactions and proving useful for bioassays and cell-imaging applications.
Article
Chemistry, Multidisciplinary
Shaohua Xu, Yueliang Wang, Yuanyuan Yao, Lifen Chen, Jiahui Xu, Bin Qiu, Longhua Guo
Summary: This study proposed a highly sensitive strategy for miRNA-21 detection using programmable toehold-mediated strand displacement (TMSD) and dark-field microscopy imaging. The method achieved highly specific recognition and amplification of miRNA-21 through the hybridization of assist strands and substrate strand. By modifying glucose oxidase (Gox) on the assist strands, the size and scattering intensity of Au@Ag NCs were reduced, allowing for the quantitative detection of miRNA-21.
Article
Multidisciplinary Sciences
Chih-Ping Mao, Shih-Chin Wang, Yu-Pin Su, Ssu-Hsueh Tseng, Liangmei He, Annie A. Wu, Richard B. S. Roden, Jie Xiao, Chien-Fu Hung
Summary: Characterizing individual biomarker protein molecules in patient blood samples using single-molecule imaging techniques holds promise for early disease diagnosis and effective treatment. However, current methods have detection limits that are orders of magnitude higher than needed, making them unsuitable for clinical use. The development of SMAC, with its ability to quantify and characterize protein molecules down to the subfemtomolar range, presents a new avenue for noninvasive disease profiling with single-molecule imaging.
Article
Chemistry, Multidisciplinary
Takanori Harashima, Yusuke Hasegawa, Satoshi Kaneko, Yuki Jono, Shintaro Fujii, Manabu Kiguchi, Tomoaki Nishino
Summary: Direct monitoring of single-molecule reactions is a promising means of mechanistic investigation, but interference from extraneous processes like bulk diffusion complicates the resolution of reaction pathways. In a study on DNA hybridization on a metal surface, single-molecule kinetic investigations successfully revealed elementary processes and showed an increase in hybridization efficiency with higher DNA concentration, contrary to previous knowledge from ensemble measurements. The derived rate constants provide a rational explanation for findings such as the suppression of DNA melting on surfaces with higher DNA coverage.
Article
Chemistry, Analytical
Zezhou Yang, Yu Guo, Jie Zhou, Fang Liu, Wenbin Liang, Yaqin Chai, Zhaohui Li, Ruo Yuan
Summary: In this study, an innovative fluorescent sensor was developed for precise and ultrasensitive detection and imaging of target miRNA-21. The sensor utilized a dextrous target-motivated polymerization/nicking DNA nanomachineries and a hyperbranched rolling circle amplification (HB-RCA)-assisted multiposition strand displacement reaction (SDR) signal amplification approach. The technique achieved target recycling through polymerization/nicking DNA nanomachineries and HB-RCA amplification induced by the released transformation target. HB-RCA was exploited to increase the local concentration and collision efficiency of the templates and primers, generating multiple repeated DNA sequences as initiators for SDR, which improved the signal amplification multiplier. The technique effectively distinguished target miRNA-21 and detected drug-manipulative miRNA expression level abnormities. The proposed cascade nucleic acid amplification strategy could offer a promising pathway for ultrasensitive imaging of diverse biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Shijun Cai, Jiaoli Wang, Jing Li, Bing Zhou, Chunmei He, Xiangxian Meng, Jin Huang, Kemin Wang
Summary: In this study, a novel AuNP-free FRET nanoflare for intracellular ATP imaging was developed based on a DNA nanostructure, self-assembled through cyclic U-type hybridization involving a certain number of DNA strands. This new nanoflare addresses the issues of cytotoxicity and time-consuming preparation associated with AuNP-based FRET probes.
CHEMICAL COMMUNICATIONS
(2021)
Review
Chemistry, Multidisciplinary
Bin Dong, Nourhan Mansour, Teng-Xiang Huang, Wenyu Huang, Ning Fang
Summary: Recent progress has been made in using single molecule fluorescence microscopy imaging to study nanoconfinement in porous materials, revealing static and dynamic heterogeneities between seemingly equal molecules by removing ensemble averaging effects. These studies allow for the investigation of various physicochemical processes at nanometer spatial resolution and provide the ability to quantitatively measure the inhomogeneities of nanoconfinement effects.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Chemistry, Multidisciplinary
Kalani M. Wijesinghe, Mazhar A. Kanak, J. Chuck Harrell, Soma Dhakal
Summary: A DNA-based FRET sensor has been developed for ultrasensitive detection of miRNAs, enabling early diagnosis of diseases.
Article
Chemistry, Multidisciplinary
Kalani M. Wijesinghe, Mazhar A. Kanak, Soma Dhakal, J. Chuck Harrell
Summary: In this study, a DNA-based FRET sensor was developed for ultrasensitive detection of a miRNA biomarker expressed by TNBC cells. The sensor showed high specificity, low detection limit, and a large dynamic range, making it suitable for detecting different miRNAs and enabling early diagnosis and screening of other genetic disorders.
Article
Biology
Jigmi Basumatary, Neptune Baro, Prakash Joshi, Partha Pratim Mondal
Summary: Single-molecule localization microscopy (SMLM) is a powerful technique that improves spatial resolution beyond the diffraction limit and allows for the analysis of biological features at the nanoscale. This study introduces a single molecule-based scanning SMLM (scanSMLM) system that enables rapid volume imaging. By rapidly changing the aperture of an electrically-tunable lens, the system achieves selective imaging of the axial object plane, allowing for volume scanning. The technique is employed to visualize the architecture of actin-filaments, the distribution of Meos-Tom20 molecules on the mitochondrial membrane, and the clustering of Hemagglutinin (HA) protein single molecules for studying Influenza-A disease progression. The system enables 3D visualization of HA distribution, providing unprecedented insights into the disease progression at the single-molecule level.
COMMUNICATIONS BIOLOGY
(2023)
Review
Chemistry, Physical
Zhuoyu Peng, Ruixuan Wan, Bo Zhang
Summary: Single-molecule fluorescence microscopy is an effective technique for studying redox processes at the electrochemical interface. It allows for high-resolution imaging of individual molecules and the detection of single nanobubbles generated on the electrode surface.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
