Article
Biophysics
Seigo Tateo, Hiroyuki Shinchi, Hikaru Matsumoto, Nonoka Nagata, Masahito Hashimoto, Masahiro Wakao, Yasuo Suda
Summary: In this study, scFv-fluorescent nanoparticle conjugates were developed for efficient labeling of scFv. The scFv was immobilized onto cadmium-free ZnS-AgInS2/ZnS core/shell nanoparticles via the interaction of nickel ions on nitrilotriacetic acid and hexahistidine (His-tag). The scFv-FNPs showed selective binding to cancer cells and can be used as bioanalytical tools.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Analytical
Surisa Sa-nguanprang, Anukorn Phuruangrat, Opas Bunkoed
Summary: A nanocomposite fluorescent probe was developed for the simultaneous determination of florfenicol and sparfloxacin through fluorescence quenching. The probe consisted of nitrogen-doped graphene quantum dots, cadmium telluride quantum dots, and zinc oxide nanoparticles integrated into a molecularly imprinted polymer. The probe showed high sensitivity and specificity, with good linear relationships and low limits of detection for both analytes. It was successfully applied to determine these antibiotics in food samples with good accuracy, precision, and recovery rates.
Article
Chemistry, Multidisciplinary
Shouzhen Li, Junfei Ma, Xuelin Zhao, Peide Zhu, Meng Xu, Yingchun Niu, Dixian Luo, Quan Xu
Summary: In this study, N-doped Ta4C3 quantum dots with high fluorescence quantum yield were synthesized for the first time. These quantum dots showed promising applications in liquid ion sensing and solid mechanics sensing.
CHINESE CHEMICAL LETTERS
(2022)
Article
Spectroscopy
Like Yang, Jia Ge, Demiao Ma, Jinlu Tang, Hongqi Wang, Zhaohui Li
Summary: In this study, a unique fluorescence biosensor based on MoS(2 )quantum dots was developed for methotrexate assay. The fluorescence of MoS(2 )QDs was quenched by methotrexate due to the inner filter effect (IFE). The IFE-based method showed a linear relationship between MoS(2 )QDs fluorescence intensity and methotrexate concentration in the range of 0.05-1 μM.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Xin Zhang, Bo Tang, Yansong Li, Chengbin Liu, Pengfei Jiao, Yuping Wei
Summary: A new strategy for designing molecularly imprinted magnetic fluorescent nanocomposites for selective fluorescence detection of target proteins has been proposed. The multifunctional nanocomposite with optics, magnetism, and biocompatibility has shown promising results in the detection of lysozyme in biological samples.
Article
Chemistry, Inorganic & Nuclear
Shuang Ding, Ying Gao, Binshan Ni, Xiaodong Yang
Summary: This study reports the environmentally friendly synthesis of Carbon Quantum Dots (CQDs) using various typical crop biomasses as carbon sources. The resulting CQDs exhibit strong fluorescence and good dispersion in water, making them suitable as fluorescent probes for the selective and sensitive detection of Fe3+ ions.
INORGANIC CHEMISTRY COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Yunchun Li, Wanxia Jia, Zhouping Yang, Jun Yang, Yinyin Chen, Jianqing Yang, Wei Zhang, Changfang Lu, Xianxiang Wang
Summary: In this study, sulfur quantum dot (COS-S QDs) nanozyme with excellent fluorescence and peroxidase activity was successfully synthesized with the assistance of chitosan oligosaccharide. The concentration of MoO42- was accurately detected by the changes in fluorescence intensity and absorbance, and satisfactory results were obtained for its determination in toiletries and cigarettes.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Article
Multidisciplinary Sciences
Cassandra M. Stawicki, Torri E. Rinker, Markus Burns, Sonal S. Tonapi, Rachel P. Galimidi, Deepthi Anumala, Julia K. Robinson, Joshua S. Klein, Parag Mallick
Summary: This study presents a straightforward method for preparing fluorescent nanoparticle probes using commercially available reagents and common laboratory equipment, enhancing their performance for biological research. The functionalized nanoparticles with DNA aptamers showed high affinity and stability, making them suitable for various biological experiments.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Hong Zhuang, Xinyu Jiang, Sijia Wu, Shujin Wang, Yong Pang, Yanjun Huang, Haiyang Yan
Summary: In this study, biomolecule-functionalized fluorescent gold nanoclusters were synthesized as a probe for sensitive detection of Cu2+. The detection conditions were pH 6 and 30 degrees Celsius, with a minimum detection limit of 52 nmol/L.
SCIENTIFIC REPORTS
(2022)
Article
Materials Science, Multidisciplinary
Yanbin Zhang, Lu-Lu Qiao, Zhi-Qiang Zhang, Yong-Fang Liu, Lin-Song Li, Huaibin Shen, Mei-Xia Zhao
Summary: Mitochondria play a crucial role in numerous cellular functions, making it important to conduct long-term stable studies of their activity. However, current commercial mitochondrial probes have poor stability, hindering their ability to trace mitochondria. In this study, a novel mitochondrial fluorescent probe was designed using InP quantum dots as a fluorescent marker, combined with TPP for specific recognition. The bespoke InP quantum dots probes demonstrated several advantages, including simple and low-cost synthesis, high quantum yields, extremely low toxicity, good biocompatibility, and excellent photochemical stability.
MATERIALS & DESIGN
(2022)
Article
Nanoscience & Nanotechnology
Sachin Kadian, Narendra Chaulagain, Naveen Narasimhachar Joshi, Kazi M. Alam, Kai Cui, Karthik Shankar, Gaurav Manik, Roger J. Narayan
Summary: A new type of heavy-metal free single-element nanomaterial, sulfur quantum dots (SQDs), has attracted significant attention for its advantages in biomedical and optoelectronic applications. A novel optimized synthesis strategy using probe sonication and heating is proposed, which reduces the reaction time from 125 hours to 15 minutes. The as-synthesized SQDs exhibit excellent aqueous solubility, photostability, and a high photoluminescence quantum yield without any post-treatment, and show excitation-dependent emission and stability in different pH and temperature environments.
Article
Chemistry, Multidisciplinary
Cara E. Bradsher, Cayla D. Ontko, Alexandra C. Koziel, James R. McBride, Sandra J. Rosenthal
Summary: This research has found that ferroelectric behavior can be achieved in nanoparticles through ion exchange, with fluorescence retention achieved through CdS shelling before ion exchange, containing defects in the CdS shell rather than the fluorescent CdSe cores. The ferroelectric response remains constant with an increase in the number of CdS monolayers, while fluorescence retention also increases, reaching 99% at 8 monolayers, allowing for the addition of ferroelectric applications to the growing list of quantum dot applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Physical
Yongfeng Liu, Xiaosheng Tang, Ming Deng, Tao Zhu, Ludvig Edman, Jia Wang
Summary: This study reports a one-step aqueous synthesis of more benign and hydrophilic AgInZnS quantum dots as a fluorescent probe for the detection of Cd2+ in aqueous solutions, with a detection range of 0.1-290 mu M and a lower limit of detection of 37.8 nM. The excellent turn-on detection of Cd2+ is attributed to the passivation of surface defects on the AgInZnS quantum dots by Cd2+, resulting in a prolonged fluorescent lifetime and increased photoluminescence quantum yield. Additionally, the AgInZnS quantum dot probe is capable of detecting Cd2+ in lake water samples and meets the WHO standard.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Chemistry, Analytical
Xiudan Hou, Jiying Song, Qi Wu, Haitao Lv
Summary: A fluorescent chiral recognition method based on CCQDs was developed to distinguish Ile enantiomers effectively. The method can quantitatively detect L-Ile and determine the percentage of enantiomers accurately.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Zahra Karimzadeh, Afshin Gharekhani, Elaheh Rahimpour, Abolghasem Jouyban
Summary: A synchronous fluorescence spectroscopy (SFS) sensor for pethidine detection was developed using UiO-66 metal-organic frameworks (MOFs) modified with N-doped carbon quantum dots (N-CQDs) embedded in hydrogel nanocomposites. N-CQDs were successfully deposited in the pores of the UiO-66 network and used as a sensitive segment. UiO-66 was employed for sensing the bonding interactions between N-CQDs and pethidine, resulting in quenching the SFS intensity of UiO-66. The nanocomposite hydrogel showed two well-resolved emission peaks corresponding to N-CQDs and UiO-66 and was used for ratiometric detection of pethidine with a low limit of detection.
Article
Neurosciences
Fatemeh Khani-Habibabadi, Leila Zare, Mohammad Ali Sahraian, Mohammad Javan, Mehrdad Behmanesh
Summary: The study investigated the role of long non-coding RNAs in regulating BDNF gene expression and OPC cell maturation, demonstrating significant correlations between Malat1 and Hotair with BDNF in glial cells. Fingolimod treatment was found to enhance OPC myelination and Malat1 may play a role in the last stages of intrinsic OL myelination, regardless of treatment.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Alireza Allafchian, Mohammad Fathi, Seyed Amir Hossein Jalali
Summary: Hybrid nanofibrous 3D scaffolds containing Aloe vera, polyvinyl alcohol, and tetracycline hydrochloride are fabricated by electrospinning for cell culture applications. The polysaccharides in Aloe vera gel enhance the biocompatibility of the scaffolds. The scaffolds show favorable fiber diameter, tensile strength, and inhibitory effect against bacteria, making them promising for cell growth.
Article
Biochemistry & Molecular Biology
Hanieh Hadady, Fereshteh Karamali, Fatemeh Ejeian, Sareh Soroushzadeh, Mohammad Hossein Nasr-Esfahani
Summary: This study evaluated two methods (micro-electromechanical systems and ultrafiltration) for isolating extracellular vesicles (EVs) from stem cells from apical papilla (SCAP). It found that different methods enriched different subsets of EVs, which significantly impacted their biological and functional effects. Further experiments confirmed the neuroprotection and anti-inflammatory capacity of SCAP-EVs in vitro, as well as their potential therapeutic effects in a retinal degeneration model.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Parisa Nadri, Saeid Ansari-Mahyari, Farnoosh Jafarpour, Amir Hossein Mahdavi, Nima Tanhaei Vash, Liana Lachinani, Kianoush Dormiani, Mohammad Hossein Nasr-Esfahani
Summary: The addition of melatonin to the culture medium can accelerate blastocyst formation and improve the quality of ovine SCNT embryos by reducing oxidative stress, apoptosis, and promoting telomere elongation. These findings offer new possibilities for the development of ovine SCNT embryos.
Article
Andrology
Fatemeh Karevanpour, Marziyeh Tavalaee, Fatemeh Kazeminasab, Milad Abdollahi, Samaneh Shirkhani, Mohsen Rahmani, Kamran Ghaedi, Sayed Mohammad Marandi, Mohammad Hossein Nasr-Esfahani
Summary: This study investigated the effects of green coffee and chlorogenic acid as active ingredients, as well as exercise, on spermatogenesis and sperm function in pre-diabetic mice. The results showed that pre-diabetic status had adverse effects on spermatogenesis and sperm quality. Exercise improved sperm motility, while green coffee supplementation reduced DNA damage and chlorogenic acid supplementation significantly improved motility. These findings suggest potential approaches for improving sperm quality in individuals with diabetes.
Article
Plant Sciences
Nikoo Azad, Hassan Ebrahimzadeh, Mehrdad Behmanesh, Vahid Niknam
Summary: Given the health risks and environmental damages caused by synthetic herbicides and fertilizers, it is important to understand how plants respond to naturally derived bioactive substances. This study investigates the effects of certain benzoic acid derivatives on spearmint growth and the role of nitric oxide in alleviating growth inhibition. The results show that some phenolic acids inhibit growth while others stimulate growth and increase the accumulation of secondary metabolites.
JOURNAL OF PLANT NUTRITION
(2023)
Article
Biology
M. Zanjirband, M. Baharlooie, Z. Safaeinejad, M. H. Nasr-Esfahani
Summary: This study investigated the novel therapeutics targets in PCOS by analyzing the transcriptome of granulosa cells using a drug repositioning approach. The results could provide valuable insights for the management of PCOS.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Multidisciplinary Sciences
Marjan Sadeghi, Mohsen Rahimi Andani, Mehdi Hajian, Nafiseh Sanei, Reza Moradi-Hajidavaloo, Nasrin Mahvash, Farnoosh Jafarpour, Mohammad Hossein Nasr-Esfahani
Summary: The role of the WNT/beta-catenin signaling pathway in preimplantation goat development was investigated. The expression of beta-CATENIN was observed in IVF and SCNT embryos, and inhibition of beta-catenin resulted in increased blastocyst formation rate. Different localization patterns of beta-catenin were observed in IVF and SCNT blastocysts. Inhibition of WNT signaling during the compact morula to blastocyst transition improved preimplantation embryonic development.
Article
Obstetrics & Gynecology
Aram Minas, Mazdak Razi, Mohammad Hossein Nasr-Esfahani, Seyed Mohammad Hashemi-Asl, Marziyeh Tavalaee
Summary: Varicocele (VCL) induces severe oxidative stress in testicular tissue, leading to infertility in 35% of males. This study compares the impact of varicose vessels on oxidative DNA damage and antioxidant reactions in the seminiferous tubules. The results show that varicocele causes significant DNA damage, reduces antioxidant reactions, and negatively affects the cell cycle progression.
REPRODUCTIVE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maryam Mirani, Soghra Bahmanpour, Fatemeh Masjedi, Zahra Derakhshan, Mahintaj Dara, Mohammad Hossein Nasr-Esfahani, Seyed Mohammad Bagher Tabei
Summary: This study reveals the negative effects of AGEs-RAGE axis on steroidogenesis in luteinized GCs, which can be mitigated by PM treatment. Understanding the interaction between AGEs and PM in ovarian physiology and pathology may lead to more targeted therapy for ovarian dysfunction.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Multidisciplinary Sciences
Ali Moghimi-Khorasgani, Farshad Homayouni Moghadam, Mohammad Hossein Nasr-Esfahani
Summary: Microglial cells (MGCs) are resident macrophages in the brain and spinal cord, playing a crucial role in immune defense and transition between different states. Increased Nurr1 expression is associated with anti-neuroinflammatory responses. This study investigates the potential role of ferulic acid (FA) in facilitating microglia transition by inducing Nurr1 expression.
Article
Obstetrics & Gynecology
Saeideh Abdollahzadeh, Ahmad Riasi, Marziyeh Tavalaee, Farnoosh Jafarpour, M. H. Nasr-Esfahani
Summary: An imbalance between omega-6 and omega-3 fatty acids in sperm is correlated with lipid peroxidation and DNA damage in sperm, indicating a possible link to fertility potential. This study found that men with high DNA fragmentation had higher levels of saturated, monounsaturated, and omega-6 to omega-3 fatty acid ratios in sperm and seminal plasma compared to men with low DNA fragmentation. Additionally, there were positive associations between the omega-6 to omega-3 fatty acid ratio and sperm lipid peroxidation, DNA fragmentation, and residual histones in both sperm and seminal plasma.
REPRODUCTIVE SCIENCES
(2023)
Article
Multidisciplinary Sciences
M. Zanjirband, R. Hodayi, Z. Safaeinejad, M. H. Nasr-Esfahani, R. Ghaedi-Heydari
Summary: This study investigated the impact of the p53 pathway in enhancing apoptosis and abnormal function of granulosa cells. Results suggest that p53 target genes play a critical role in the pathogenesis of PCOS, specifically through the enhancement of apoptosis in granulosa cells.
SCIENTIFIC REPORTS
(2023)
Article
Environmental Sciences
Ali Seraj, Alireza Allafchian, Fathallah Karimzadeh, Arian Valikhani, Seyed Amir Hossein Jalali
Summary: In this study, an enhanced antibacterial agent was developed by inlaying silver nanoparticles (AgNPs) on a kappa-carrageenan (KC) base and coating them on Fe3O4 magnetic cores (Fe3O4@KC@Ag). The resulting Fe3O4@KC@Ag nanocomposites showed improved antibacterial activity due to the decreased agglomeration of AgNPs. These findings suggest that Fe3O4@KC@Ag nanocomposites could be promising agents for combating bacterial infections in aqueous environments.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemical Research Methods
Behrouz Aghajanloo, Fatemeh Ejeian, Francesca Frascella, Simone L. L. Marasso, Matteo Cocuzza, Alireza Fadaei Tehrani, Mohammad Hossein Nasr Esfahani, David W. W. Inglis
Summary: Deterministic lateral displacement (DLD) is a passive separation method for particle and cell sorting. It offers high size resolution and operates using capillary pressure, making it suitable for point-of-care devices. The method achieves high separation efficiency and is simple to use, making it a valuable technique in the field of microfluidic technologies.
