A novel polypeptide-modified fluorescent gold nanoclusters for copper ion detection

Biomolecule-functionalized fluorescent gold nanocluster (AuNCs) have attracted a lot of attention due to good biocompatibility, stable physicochemical properties and considerable cost advantages. Inappropriate concentration of Cu2+ may cause a variety of diseases. In this study, AuNCs were synthesized in alkaline aqueous solution using bovine serum albumin (BSA) as a template. And then, the peptide CCYVVDAHRDY was coupled to AuNCs. Furthermore, the fluorescence of synthesized CCYWDAHRDY-AuNCs response to Cu2+ was evaluated. As the results shown that the CCYWDAHRDY-AuNCs can sensitively detect Cu2+. After adding Cu2+ to the probe system, the fluorescence of the CCYWDAHRDY-AuNCs was quenched. The detection conditions were at pH 6 and 30 degrees C for 10 min, the linear relationship between Cu(2+ )concentration and fluorescence intensity were good in the range of 0.1 similar to 4.2 mu mol/L. The regression equation was y = - 105.9x +693.68, the linear correlation coefficient is 0.997, and the minimum detection limit was 52 nmol/L.

Automated summary

In this study, biomolecule-functionalized fluorescent gold nanoclusters were synthesized as a probe for sensitive detection of Cu2+. The detection conditions were pH 6 and 30 degrees Celsius, with a minimum detection limit of 52 nmol/L.