Article
Biochemical Research Methods
Lisa Monika Klein, Angelika Miriam Gabler, Michael Rychlik, Christoph Gottschalk, Florian Kaltner
Summary: This study developed a sensitive analytical approach for simultaneous detection and quantification of unsaturated pyrrolizidine alkaloids, their corresponding N-oxides, and tropane alkaloids in milk of dairy cows. The method showed low limits of detection and quantification, good recovery rates and repeatability. Traces of pyrrolizidine alkaloids were detected in three milk samples obtained from milk vending stations and local marketers in Bavaria, Germany.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Nuria Leon, Pablo Miralles, Vicent Yusa, Clara Coscolla
Summary: A green analytical method has been developed to determine the levels of 30 tropane and pyrrolizidine alkaloids and their N-oxides in dried teas and herbs. The method combines the QuEChERS procedure with LC-Q-Orbitrap HRMS analysis. The method has been validated and shown to have satisfactory performance in different tea and herb matrices. The results also demonstrate that the analyzed tea and herb samples comply with European regulations.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Food Science & Technology
Alejandro Garcia-Juan, Nuria Leon, Sergio Armenta, Olga Pardo
Summary: This study successfully developed and validated an analytical method for the simultaneous determination of ergot, tropane, and pyrrolizidine alkaloids in cereal-based food. The method showed high recoveries and precision, meeting the validation requirements. Analysis of reference materials further confirmed the reliability of the method. Analysis of actual samples revealed the presence of ergot alkaloids and pyrrolizidine alkaloids in some samples.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Haolei Han, Changling Jiang, Chen Wang, Ziqi Wang, Yunfeng Chai, Xiangchun Zhang, Xin Liu, Chengyin Lu, Hongping Chen
Summary: A sensitive method was developed to simultaneously detect 14 PAs/PANOs in teas and weeds using UHPLC-MS/MS. The pollution levels and patterns of PAs/PANOs were significantly different in various types of teas, suggesting that the source of PAs/PANOs in teas may be related to weeds in tea gardens.
Article
Chemistry, Applied
Oliver Keuth, H. U. Humpf, Peter Fuerst
Summary: A method combining automated solid-phase extraction clean-up and Ultra-Performance Liquid Chromatography/Tandem Mass Spectrometry detection was developed to determine pyrrolizidine alkaloids (PA) in various food commodities. Survey results showed that some honey and tea samples contained PA, with levels reaching up to 595ug/kg.
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
(2022)
Article
Chemistry, Applied
Serena Rizzo, Rita Celano, Anna Lisa Piccinelli, Mariateresa Russo, Luca Rastrelli
Summary: An analytical procedure for the screening and determination of pyrrolizidine alkaloids (PAs) in food supplements, herbal infusions, honey, and teas was successfully validated. The study found that 10% of the analyzed samples were contaminated above the maximum levels (MLs) set by European legislation. Contamination of herbal infusions of mixed plants raised potential health concerns, with 23% showing PA levels above ML. Although a high number of PAs not included in the regulation were detected in honey and herbal food supplements, their contribution to overall levels in honey was not significant.
Article
Chemistry, Applied
Hyoyoung Kim, Eun Joo Baek, Yong-Kyoung Kim, Hyejin Park, Suel Hye Hur, Jang-Eok Kim, Ho Jin Kim
Summary: In this study, a specific, reliable, and suitable method for a fast and simultaneous analysis of 335 pesticides in ginseng was developed using liquid chromatography quadrupole mass spectrometry (LC-MS/MS) and gas chromatography quadrupole mass spectrometry (GC-MS/MS). The method was validated for linearity, sensitivity, selectivity, accuracy, and precision, with limits of detection (LOD) and limits of quantification (LOQ) ranging from 0.1-5.8 μg/kg and 0.3-17.5 μg/kg, respectively. Analysis of 467 ginseng samples from 2016 to 2019 revealed pesticide residues in 304 samples, but most were below the standard. The hazard quotient (HQ) of ginseng for detected pesticides was less than 1, indicating low risk.
Article
Biochemical Research Methods
Ryan W. Pearce, Jillian Kodger, Yana Sandlers
Summary: This study developed an LC-ESI-MS/MS method for analyzing various cellular acyl-CoA in a single injection, with a quick extraction step and compensation for matrix effects through isotopically labeled internal standards and matrix-matched calibration. The method was successfully applied to identify 21 cellular acyl-CoA in SK-HEP-1 cells and investigate alterations in acyl-CoA levels post Mito Q antioxidant intervention.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Applied
Lorena Lucatello, Roberta Merlanti, Luciana De Jesus Inacio, Vittoria Bisutti, Ludovica Montanucci, Francesca Capolongo
Summary: The study used liquid chromatography tandem mass spectrometry to analyze 775 honey samples from different sources, revealing lower alkaloid contamination in local beekeepers' honey compared to that from large retailers. The results indicated that local honey samples do not pose a double health risk compared to products from large retailers.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Medical Laboratory Technology
Jordi Farre-Segura, Neus Fabregat-Cabello, Chiara Calaprice, Laurent Nyssen, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: A rapid and specific LC-MS/MS method for glucagon quantitation has been developed and validated, showing stability and accuracy in comparison to other immunoassay methods. This method could potentially establish a standardized approach for glucagon analysis in routine care settings.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Delia Castilla-Fernandez, David Moreno-Gonzalez, Juan F. Garcia-Reyes, Evaristo Ballesteros, Antonio Molina-Diaz
Summary: Datura species are known for their high concentration of tropane alkaloids, which can lead to poisoning when accidentally mixed with edible crops. Testing on frozen spinach and spinach-based infant food products found that some samples had levels close to the maximum limits set by the European Union. This highlights the global concern over the presence of these compounds in various commodities.
Article
Biochemical Research Methods
Martins Jansons, Deniss Fedorenko, Romans Pavlenko, Zane Berzina, Vadims Bartkevics
Summary: A method for the determination of pyrrolizidine alkaloids in tea, honey, herbal tinctures, and milk samples was developed using nano-LC-MS with high-resolution Orbitrap mass spectrometry. The method demonstrated the occurrence of pyrrolizidine alkaloids in 33% of honey samples and 47% of tea samples, exceeding the maximum limit in tea samples. The developed method showed advantages in sensitivity and solvent consumption reduction.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Anran Sheng, Qingqing Chen, Mengqi Yu, Ruiqi Xiao, Tianji Zhang, Zhiyu Wang, Robert J. Linhardt, Xiaojun Sun, Lan Jin, Lianli Chi
Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Sophie Catanese, Raoul K. Khanna, Antoine Lefevre, Hugo Alarcan, Pierre-Jean Pisella, Patrick Emond, Helene Blasco
Summary: To apply metabo-lipidomics in ophthalmological and systemic diseases, it is necessary to standardize the preanalytical and analytical steps in human tears. This study compared different methods for each step of the workflow and found that methanol/water extraction provided the best results for metabolomics, while Folch extraction was superior for lipidomics. The use of microcapillary glass tube for sampling was superior to Schirmer strip for metabolomics. A volume of 5 mu L provided satisfactory metabo-lipidomic coverage. These findings will contribute to improving the metabo-lipidomic workflow in human tears for the identification of new biomarkers.
Article
Biochemical Research Methods
Masaki Takiwaki, Fumio Nomura, Mamoru Satoh, Sachio Tsuchida, Kazuo Otake, Junko Takagi
Summary: This study aimed to develop an LC/MS/MS-based method for detecting human plasma AVP and compare it with the traditional RIA method. Although there was a weak overall correlation between the results obtained using the two different methods, disagreements were observed at low concentrations.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Chemistry, Applied
Larissa E. Pferdmenges, Annett Schroeter, Regina Lohmayer, Lisa Striegel, Michael Rychlik, Alexandra Mueller, Ann-Katrin Meinhardt, Bernhard Trierweiler, Bernd M. Hartmann, Lara Frommherz
Summary: This study revealed significant differences in nutrient composition among different German beer styles, providing an improved basis for understanding the nutritional value of beer. The new data showed higher daily nutrient recommendation percentages for niacin, vitamin B6, and folate, but lower values for alcohol and elements like copper and iodine for the average beer consumer. These findings demonstrate the importance of updated data for future studies on nutritional value calculation in beer consumption.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Food Science & Technology
Anne-Margarethe Enge, Florian Kaltner, Christoph Gottschalk, Angelina Kin, Michael Kirstgen, Joachim Geyer, Anja These, Helen Hammer, Oliver Poetz, Albert Braeuning, Stefanie Hessel-Pras
Summary: This study investigates the transporter-mediated uptake of PAs and its implications in PA toxicity, finding that treatment with PAs affects transporter expression levels in HepaRG cells. Knockdown of the two uptake transporters results in reduced PA uptake and attenuated cytotoxic effects of PAs in a time-dependent manner. The results confirm the importance of toxicokinetic studies for the risk assessment of PAs.
MOLECULAR NUTRITION & FOOD RESEARCH
(2022)
Article
Food Science & Technology
Felix Hoheneder, Eva Maria Biehl, Katharina Hofer, Johannes Petermeier, Jennifer Groth, Markus Herz, Michael Rychlik, Michael Hess, Ralph Hueckelhoven
Summary: The epidemiology of Fusarium Head Blight (FHB) in spring barley was assessed in this study. Soil and spray inoculation were used to increase disease pressure and provoke genotypic differentiation. The evaluation of stable quantitative FHB resistance in barley genotypes was achieved through the quantification of fungal DNA contents and multiple Fusarium toxins. The study also identified the impact of weather conditions on fungal colonization in mature barley grain.
Article
Food Science & Technology
Maria Z. Tsimidou, Stella A. Ordoudi, Fani Th Mantzouridou, Nikolaos Nenadis, Tamara Stelzl, Michael Rychlik, Nastasia Belc, Claudia Zoani
Summary: The pan-European distributed research infrastructure for promoting metrology in food and nutrition, known as METROFOOD-RI, aims to provide high-quality metrology services across the food chain. It includes physical and electronic facilities and is currently preparing to apply for ERIC legal status. METROFOOD-RI partners have identified key thematic areas and strategic priorities to contribute to global societal challenges and emergencies.
Article
Agriculture, Multidisciplinary
Florian Kaltner
Summary: Toxic plant alkaloids (TPAs) found in food may be affected by storing or processing, leading to decreased levels of the parent alkaloids. However, little is known about potential transformation products and their toxic potential. This perspective summarizes the current knowledge on the behavior of opium, pyrrolizidine, and tropane alkaloids during processing or storing, and highlights analytical strategies to identify and elucidate potential transformation products.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Mycology
Sophie Scheibenzuber, Fabian Dick, Marina Brettraeger, Martina Gastl, Stefan Asam, Michael Rychlik
Summary: A liquid chromatography tandem mass spectrometry (LC-MS/MS) multi-mycotoxin method was developed for the analysis of multiple Alternaria toxins in barley and malt. The method employed stable isotope dilution analysis and matrix-matched calibration for quantitation. The study revealed the potential formation of mycotoxins during the malting process.
MYCOTOXIN RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Lance Buckett, Sabrina Schoenberger, Veronika Spindler, Nadine Sus, Christian Schoergenhofer, Jan Frank, Oliver Frank, Michael Rychlik
Summary: This study presents a synthetic approach to obtain reference standards for the identification of human xanthohumol metabolites. The metabolites were analyzed using qTOF LC-MS/MS, and some were matched to a human blood sample. Isomers of the reference standards were also identified. The methods used in this study unequivocally identified the metabolites of xanthohumol in the blood circulatory system.
Article
Biochemistry & Molecular Biology
Julia Buchmueller, Florian Kaltner, Christoph Gottschalk, Maria Maares, Albert Braeuning, Stefanie Hessel-Pras
Summary: This study aims to elucidate the toxicokinetic parameters of PAs, including their uptake and metabolism, using in vitro models. The results show that PAs pass through the intestinal barrier and reach the liver, where they are metabolized into reactive metabolites that interact with nucleophilic centers. These findings contribute to a better understanding of the molecular processes underlying the observed effects of PAs in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Lisa Monika Klein, Angelika Miriam Gabler, Michael Rychlik, Christoph Gottschalk, Florian Kaltner
Summary: This study developed a sensitive analytical approach for simultaneous detection and quantification of unsaturated pyrrolizidine alkaloids, their corresponding N-oxides, and tropane alkaloids in milk of dairy cows. The method showed low limits of detection and quantification, good recovery rates and repeatability. Traces of pyrrolizidine alkaloids were detected in three milk samples obtained from milk vending stations and local marketers in Bavaria, Germany.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Correction
Biochemical Research Methods
Lisa Monika Klein, Angelika Miriam Gabler, Michael Rychlik, Christoph Gottschalk, Florian Kaltner
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Sabir Khan, Ademar Wong, Michael Rychlik, Maria del Pilar Taboada Sotomayor
Summary: This study developed a novel voltammetric sensor for the quantitation of folate in foodstuffs. The sensor, made from magnetic molecularly imprinted polymers (MMIPs), showed high sensitivity and selectivity. The sensor successfully detected folate in different food samples with high recovery percentages. The results demonstrated the efficiency and reliability of the biomimetic sensor for folate determination in real food samples.
Article
Food Science & Technology
Marina Brettraeger, Sophie Scheibenzuber, Stefan Asam, Michael Rychlik, Martina Gastl, Thomas Becker
Summary: Brewing experiments were conducted to assess the impact of black-colored grain on Alternaria mycotoxin concentrations throughout the brewing process. The concentrations of 13 different Alternaria mycotoxins were analyzed in each step using liquid chromatography-tandem mass spectrometry, and the toxin concentrations were balanced over the entire process. The results showed that TeA was the only toxin to migrate into the final beer, while other toxins were mainly found in the spent grains. This study suggests that optical sorting of malt batches can be a useful tool for preventing elevated mycotoxin concentrations in the malting and brewing industry.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2023)
Article
Biochemical Research Methods
Leopold Weidner, Daniel Hemmler, Michael Rychlik, Philippe Schmitt-Kopplin
Summary: We developed DBDIpy, a Python library for processing and analyzing untargeted, time-sensitive plasma ionization MS datasets. It is capable of identifying in-source fragments and rivaling ionization pathways of the same analytes in time-sensitive datasets. DBDIpy also includes basic functions for processing untargeted metabolomics data and interfaces to existing tools for MS data analysis in Python.
Article
Biotechnology & Applied Microbiology
Yvonne Methner, Nadine Weber, Oliver Kunz, Martin Zarnkow, Michael Rychlik, Mathias Hutzler, Fritz Jacob
Summary: Nonalcoholic beers are gaining popularity due to increasing consumer awareness of healthier lifestyles. These beers, produced using non-Saccharomyces yeasts, offer a diverse range of flavors. This study found that nonalcoholic beers contain significant amounts of B vitamins, making them a potential dietary supplement.
FEMS YEAST RESEARCH
(2022)