Journal
ARCHIVES OF MICROBIOLOGY
Volume 199, Issue 2, Pages 385-390Publisher
SPRINGER
DOI: 10.1007/s00203-016-1298-1
Keywords
Bacillus thuringiensis subsp morrisoni; Thurincin H; Bacteriocin; thnR transcriptional regulator
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Funding
- (Academic Excellence) from the University of Guanajuato [004/2014, 015/2015]
- UG from the Secretary of Innovation, Science and Higher Education of Guanajuato, Mexico [103/2016]
- Catedra CONACyT, Mexico [2069]
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Thurincin H is a bacteriocin synthesized by some strains of Bacillus thuringiensis. In this study, the thurincin H genetic cassette, which contains ten genes, from a Mexican strain of B. thuringiensis subsp. morrisoni (Btm) was cloned and sequenced. To study the function of the thnR gene component in the cassette, we generated various constructs with or without thnR for expression in Btm. All transformants harboring thnR in recombinant plasmids showed a decrease of similar to 15 to similar to 90 % in inhibitory activity against the target strain, Bacillus cereus 183. Importantly, a similar to 90 % reduction in inhibition occurred with Btm harboring a construct containing thnR alone, suggesting that ThnR, indeed, functions as a negative transcription regulator of the thurincin H cassette. Based on sequence homology, ThnR was classified as a member of the YtrA subfamily of the GntR superfamily of transcriptional regulators.
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