An arsenate-reducing and alkane-metabolizing novel bacterium, Rhizobium arsenicireducens sp nov., isolated from arsenic-rich groundwater

A novel arsenic (As)-resistant, arsenate-respiring, alkane-metabolizing bacterium KAs 5-22(T), isolated from As-rich groundwater of West Bengal was characterized by physiological and genomic properties. Cells of strain KAs 5-22(T) were Gram-stain-negative, rod-shaped, motile, and facultative anaerobic. Growth occurred at optimum of pH 6.0-7.0, temperature 30 A degrees C. 16S rRNA gene affiliated the strain KAs 5-22(T) to the genus Rhizobium showing maximum similarity (98.4 %) with the type strain of Rhizobium naphthalenivorans TSY03b(T) followed by (98.0 % similarity) Rhizobium selenitireducens B1(T). The genomic G + C content was 59.4 mol%, and DNA-DNA relatedness with its closest phylogenetic neighbors was 50.2 %. Chemotaxonomy indicated UQ-10 as the major quinone; phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol as major polar lipids; C-16:0, C-17:0, 2-OH C-10:0, 3-OH C-16:0, and unresolved C-18:1 E center dot 7C/E center dot 9C as predominant fatty acids. The cells were found to reduce O-2, As5+, NO3 (-), SO4 (2-) and Fe3+ as alternate electron acceptors. The strain's ability to metabolize dodecane or other alkanes as sole carbon source using As5+ as terminal electron acceptor was supported by the presence of genes encoding benzyl succinate synthase (bssA like) and molybdopterin-binding site (mopB) of As5+ respiratory reductase (arrA). Differential phenotypic, chemotaxonomic, genotypic as well as physiological properties revealed that the strain KAs 5-22(T) is separated from its nearest recognized Rhizobium species. On the basis of the data presented, strain KAs 5-22(T) is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium arsenicireducens sp. nov. is proposed as type strain (=LMG 28795(T)=MTCC 12115(T)).