Targeted genetic screening in mice through haploid embryonic stem cells identifies critical genes in bone development

Mosaicism in CRISPR/Cas9-mediated genome editing

(2018) Maryam Mehravar et al.   DEVELOPMENTAL BIOLOGY

FOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging

(2017) Hanjun Li et al.   JOURNAL OF CLINICAL INVESTIGATION

A reversible haploid mouse embryonic stem cell biobank resource for functional genomics

(2017) Ulrich Elling et al.   NATURE

Refined sgRNA efficacy prediction improves large- and small-scale CRISPR–Cas9 applications

(2017) Maurice Labuhn et al.   NUCLEIC ACIDS RESEARCH

Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus

(2016) Cuiqing Zhong et al.   CELL RESEARCH

Leptin Receptor Promotes Adipogenesis and Reduces Osteogenesis by Regulating Mesenchymal Stromal Cells in Adult Bone Marrow

(2016) Rui Yue et al.   Cell Stem Cell

Inactivation of Regulatory-associated Protein of mTOR (Raptor)/Mammalian Target of Rapamycin Complex 1 (mTORC1) Signaling in Osteoclasts Increases Bone Mass by Inhibiting Osteoclast Differentiation in Mice

(2016) Qinggang Dai et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes

(2016) Sean Chen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Generation and application of mammalian haploid embryonic stem cells

(2016) M. Bai et al.   JOURNAL OF INTERNAL MEDICINE

Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis

(2015) Sidi Chen et al.   CELL

CRISPR-Cas9-Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library

(2015) Cuiqing Zhong et al.   Cell Stem Cell

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing

(2015) Masakazu Hashimoto et al.   Scientific Reports

RELAXIN enhances differentiation and matrix mineralization through Relaxin/insulin-like family peptide receptor 2 (Rxfp2) in MC3T3-E1 cells in vitro

(2014) Carolina Duarte et al.   BONE

When Half Is Better Than the Whole: Advances in Haploid Embryonic Stem Cell Technology

(2014) Chikara Kokubu et al.   Cell Stem Cell

Genetic Exploration of the Exit from Self-Renewal Using Haploid Embryonic Stem Cells

(2014) Martin Leeb et al.   Cell Stem Cell

Formation of Proximal and Anterior Limb Skeleton Requires Early Function of Irx3 and Irx5 and Is Negatively Regulated by Shh Signaling

(2014) Danyi Li et al.   DEVELOPMENTAL CELL

Improved vectors and genome-wide libraries for CRISPR screening

(2014) Neville E Sanjana et al.   NATURE METHODS

Generating genetically modified mice using CRISPR/Cas-mediated genome engineering

(2014) Hui Yang et al.   Nature Protocols

Easy quantitative assessment of genome editing by sequence trace decomposition

(2014) Eva K. Brinkman et al.   NUCLEIC ACIDS RESEARCH

Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA

(2014) C. Long et al.   SCIENCE

Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems

(2014) Akihiro Yasue et al.   Scientific Reports

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes

(2013) Hui Yang et al.   CELL RESEARCH

Genetic Modification and Screening in Rat Using Haploid Embryonic Stem Cells

(2013) Wei Li et al.   Cell Stem Cell

An RNA-seq protocol to identify mRNA expression changes in mouse diaphyseal bone: Applications in mice with bone property alteringLrp5mutations

(2013) Ugur M Ayturk et al.   JOURNAL OF BONE AND MINERAL RESEARCH

The microtubule-associated protein DCAMKL1 regulates osteoblast function via repression of Runx2

(2013) Weiguo Zou et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library

(2013) Hiroko Koike-Yusa et al.   NATURE BIOTECHNOLOGY

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA

(2013) Daisuke Mashiko et al.   Scientific Reports

Genome engineering of mammalian haploid embryonic stem cells using the Cas9/RNA system

(2013) Takuro Horii et al.   PeerJ

Generation of Genetically Modified Mice by Oocyte Injection of Androgenetic Haploid Embryonic Stem Cells

(2012) Hui Yang et al.   CELL

Cooperative and antagonistic roles for Irx3 and Irx5 in cardiac morphogenesis and postnatal physiology

(2012) N. Gaborit et al.   DEVELOPMENT

Standardized nomenclature, symbols, and units for bone histomorphometry: A 2012 update of the report of the ASBMR Histomorphometry Nomenclature Committee

(2012) David W Dempster et al.   JOURNAL OF BONE AND MINERAL RESEARCH

Androgenetic haploid embryonic stem cells produce live transgenic mice

(2012) Wei Li et al.   NATURE

Mutations in IRX5 impair craniofacial development and germ cell migration via SDF1

(2012) Carine Bonnard et al.   NATURE GENETICS

Use and Abuse of RNAi to Study Mammalian Gene Function

(2012) W. G. Kaelin   SCIENCE

Haploid embryonic stem cells: an ideal tool for mammalian genetic analyses

(2012) Linyu Shi et al.   Protein & Cell

Forward and Reverse Genetics through Derivation of Haploid Mouse Embryonic Stem Cells

(2011) Ulrich Elling et al.   Cell Stem Cell

Heterozygous deletion at the RLN1 locus in a family with testicular germ cell cancer identified by integrating copy number variation data with phenome and interactome information

(2011) D. Edsgärd et al.   INTERNATIONAL JOURNAL OF ANDROLOGY

Derivation of haploid embryonic stem cells from mouse embryos

(2011) Martin Leeb et al.   NATURE

Zebrafish as a unique model system in bone research: the power of genetics andin vivoimaging

(2010) K. M. Spoorendonk et al.   JOURNAL OF APPLIED ICHTHYOLOGY

The Genetic Landscape of a Cell

(2010) M. Costanzo et al.   SCIENCE

Wnt and PPARγ signaling in osteoblastogenesis and adipogenesis

(2009) Ichiro Takada et al.   Nature Reviews Rheumatology

Age- and gender-dependent changes in three-dimensional microstructure of cortical and trabecular bone at the human femoral neck

(2009) H. Chen et al.   OSTEOPOROSIS INTERNATIONAL