Article
Chemistry, Analytical
Kai Zhao, Penglu Zhao, Jianhong Dong, Yunman Wei, Bin Chen, Yanjuan Wang, Xinxiang Pan, Junsheng Wang
Summary: This study demonstrates the successful isolation of circulating tumor cells (CTCs) from blood cells using a combination of dielectrophoresis and magnetophoresis in a microfluidic chip. The results show the potential application of this method in diagnostics.
Article
Biochemical Research Methods
Kunpeng Cai, Shruti Mankar, Taiga Ajiri, Kentaro Shirai, Tasuku Yotoriyama
Summary: The study introduces a fully integrated high-throughput microfluidic circulatory fluorescence-activated cell sorting system capable of significantly increasing the purity of rare cells in a short period of time and effectively enriching different types of cells. Through a sequential sorting process and efficient contamination-free design, effective enrichment of clinical rare cells has been achieved.
Article
Chemistry, Multidisciplinary
Anil Koklu, Shofarul Wustoni, Valentina-Elena Musteata, David Ohayon, Maximilian Moser, Iain McCulloch, Suzana P. Nunes, Sahika Inal
Summary: Alzheimer's disease is a neurodegenerative disorder with no specific cure, emphasizing the importance of early diagnosis. A common pathological change in AD-affected brains is the accumulation of Aβ protein into plaques, which was detected using a micron-scale OECT in this study.
Article
Chemistry, Analytical
Haitao Zhao, Lip Ket Chin, Yuzhi Shi, Patricia Yang Liu, Yi Zhang, Hong Cai, Eric Peng Huat Yap, Wee Ser, Ai-Qun Liu
Summary: The SWANS platform utilizes microfluidic-nanophotonic technology to achieve continuous sorting of nanoscale biomolecules. Through the study of near-field optical lattice and sorting mechanism, efficient separation of mixtures is achieved.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Mechanics
Majid Gholinejad, Ali Jabari Moghadam, Seyed Ali Mousavi Shaegh
Summary: This study investigates the governing parameters of ion concentration polarization (ICP) and their effect on the preconcentration behavior of charged analytes. The results show that different electric fields can change the accumulation patterns of preconcentrated analytes. In addition, the buffer concentration only affects the enrichment factor without influencing the pattern of preconcentrated analytes.
Article
Biochemical Research Methods
Yang Bu, Jinhui Wang, Sheng Ni, Yusong Guo, Levent Yobas
Summary: Extracellular vesicles (EVs) are cell-derived bioparticles that have significant roles in various biological processes and can be potential biomarkers for pre-diagnostic applications. However, isolating EV subpopulations, especially exosomes, from biological fluids is challenging due to their small size. In this study, a microfluidic device combining electrothermal fluid rolls and dielectrophoresis was used for continuous-flow label-free size fractionation of EVs. The device showed high efficiency in separating submicron particles and isolating intact exosomes from cell culture medium or blood serum. This method provides a promising platform for the purification of target bioparticles directly from physiological fluids and may have important implications in EV-related diagnostic applications.
Article
Chemistry, Analytical
Adam Shebindu, Himali Somaweera, Zachary Estlack, Jungtae Kim, Jungkyu Kim
Summary: This study introduces a programmable microfluidic platform (PMP) combined with isotachophoresis (ITP) for fully automated optimization of multiple analytes. By utilizing lifting-gate microvalve technology, a two-dimensional microvalve array was designed and fabricated to seamlessly integrate with an ITP chip for automated buffer selection and repetitive ITP procedures. The optimal buffer combination of 40mM borate and 100mM Tris-HCl successfully preconcentrated all analytes during a single ITP run.
Review
Chemistry, Analytical
Shuang Ding, Haijun Zhang, Xuemei Wang
Summary: Lung diseases pose serious threats to human health worldwide, and traditional models are unable to accurately simulate lung conditions. Human organ-on-a-chip platforms and biosensors can better mimic lung characteristics, providing new methods for studying disease development and drug efficacy.
Article
Mechanics
Shuai Yuan, Mingyong Zhou, Xijiang Liu, Qiang Li, Dietmar Drummer, Bingyan Jiang
Summary: With the recent interest in microfluidic devices, this study focuses on preconcentration technology using field-amplified sample stacking (FASS) in microchannels. Numerical investigations reveal that reducing microchannel width is an effective way to improve enrichment quality. In addition, the study proposes a high-conductance gradient boundary to further enhance the performance of traditional FASS.
Article
Biochemical Research Methods
Yen-Ling Fang, Chih-Hung Wang, Yi-Sin Chen, Chun-Chih Chien, Feng-Chih Kuo, Huey-Ling You, Mel S. Lee, Gwo-Bin Lee
Summary: The study developed an integrated microfluidic chip for rapid isolation of bacteria from blood, aiding in early sepsis diagnosis. The device is efficient with a high capture rate of 85% and the entire detection process can be completed in just 4 hours.
Article
Biochemical Research Methods
Yan Zhang, Sungho Kim, Weihua Shi, Yaoyao Zhao, Insu Park, Christopher Brenden, Hrishikesh Iyer, Prasoon Jha, Rashid Bashir, Jonathan V. Sweedler, Yurii Vlasov
Summary: The silicon microfluidic platform allows for the monolithic integration of transparent micron-scale microfluidic channels, on-chip segmentation of analyte flows into picoliter droplets, and spatial and temporal separation of oil and aqueous phases during electro-spray for subsequent mass spectrometry analysis.
Article
Chemistry, Analytical
Mian Yang, Jorvani Cruz Villarreal, Nethmi Ariyasinghe, Rory Kruithoff, Robert Ros, Alexandra Ros
Summary: Advancements in single-cell analysis technologies are crucial for characterizing cells, studying cell biology, drug design, and cancer diagnosis and treatment. Current approaches using fluorescent antibody-binding technology are limited by high background and cross-talk from fluorescent labels, highlighting the need for novel label-free methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Xiaorui Wang, Gaowa Xing, Nan Li, Yaoshuang Xie, Ling Lin
Summary: An integrated microfluidic device combining a microfluidic chip with immunochromatographic strips was developed for rapid detection of multiple antibiotics in milk. The device achieved qualitative and quantitative analysis of four types of antibiotics using mobile phone photography and mobile phone application analysis in a 10-minute detection time. The device successfully integrated separation and real-time detection onto a chip, providing a promising perspective for the detection of multiple antibiotics in milk.
CHINESE CHEMICAL LETTERS
(2023)
Article
Chemistry, Analytical
Yuting Shang, Gaowa Xing, Xuejiao Liu, Haifeng Lin, Jin-Ming Lin
Summary: A portable microfluidic biosensor has been developed for the detection of E. coli O157:H7 using finger actuation. The biosensor consists of three functional zones and is capable of separating and detecting the target bacteria through immunomagnetic separation, nucleic acid extraction and purification, and signal detection. It offers quantitative detection and high recovery rate, eliminating the need for external pumps and skilled personnel.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Subin Mao, Catherine Fonder, Md Fazlay Rubby, Gregory J. J. Phillips, Donald S. S. Sakaguchi, Long Que
Summary: To study the effects of dopamine and serotonin on adult rat hippocampal progenitor cells, an integrated chip was developed. This chip allows the diffusion of neurotransmitters to neurospheroids through a diffusion barrier created by misaligned micropillars. By controlling the composition of the gel inside the barrier, the concentration of neurotransmitters inside the neurospheroid chamber can be increased. The chip also proves to be useful in studying the interactions between gut microbiota and the brain.
Article
Clinical Neurology
Kiran Samra, Amy M. MacDougall, Georgia Peakman, Arabella Bouzigues, Martina Bocchetta, David M. Cash, Caroline Greaves, Rhian S. Convery, John C. van Swieten, Lize Jiskoot, Harro Seelaar, Fermin Moreno, Raquel Sanchez-Valle, Robert Laforce, Caroline Graff, Mario Masellis, Carmela Tartaglia, James B. Rowe, Barbara Borroni, Elizabeth Finger, Matthis Synofzik, Daniela Galimberti, Rik Vandenberghe, Alexandre de Mendonca, Chris R. Butler, Alexander Gerhard, Simon Ducharme, Isabelle Le Ber, Pietro Tiraboschi, Isabel Santana, Florence Pasquier, Johannes Levin, Markus Otto, Sandro Sorbi, Jonathan D. Rohrer, Lucy L. Russell
Summary: This study investigated the optimal method of adding motor features to a clinical rating scale for frontotemporal dementia (FTD). The results showed that motor symptoms are present in mutation carriers at all disease stages, and including motor symptoms in a rating scale can provide a more accurate assessment of disease severity and incorporate a wider spectrum of FTD phenotypes in the same clinical trial.
JOURNAL OF NEUROLOGY
(2023)
Article
Clinical Neurology
Kiran Samra, Amy Macdougall, Georgia Peakman, Arabella Bouzigues, Martina Bocchetta, David M. Cash, Caroline Greaves, Rhian S. Convery, John C. van Swieten, Lize C. Jiskoot, Harro Seelaar, Fermin Moreno, Raquel Sanchez-Valle, Robert Laforce, Caroline Graff, Mario Masellis, Maria Carmela Tartaglia, James B. Rowe, Barbara Borroni, Elizabeth Finger, Matthis Synofzik, Daniela Galimberti, Rik Vandenberghe, Alexandre de Mendonca, Christopher R. Butler, Alexander Gerhard, Simon Ducharme, Isabelle Le Ber, Pietro Tiraboschi, Isabel Santana, Florence Pasquier, Johannes Levin, Markus Otto, Sandro Sorbi, Jonathan D. Rohrer, Lucy L. Russell
Summary: A study on frontotemporal dementia found that neuropsychiatric symptoms occur in mutation carriers at all disease stages, with hallucinations and delusions providing additional staging benefit. The inclusion of these features in rating scales could improve the evaluation of disease progression.
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2023)
Article
Clinical Neurology
Giancarlo Logroscino, Marco Piccininni, Caroline Graff, Orla Hardiman, Albert C. Ludolph, Fermin Moreno, Markus Otto, Anne M. Remes, James B. Rowe, Harro Seelaar, Eino Solje, Elka Stefanova, Latchezar Traykov, Vesna Jelic, Melissa Taheri Rydell, Niall Pender, Sarah Anderl-Straub, Myriam Barandiaran, Alazne Gabilondo, Johanna Kruger, Alexander G. Murley, Timothy Rittman, Emma L. van der Ende, John C. van Swieten, Paeivi Hartikainen, Gorana Mandic Stojmenovic, Shima Mehrabian, Luisa Benussi, Antonella Alberici, Maria Teresa Dell'Abate, Chiara Zecca, Barbara Borroni
Summary: This study examined the incidence of frontotemporal lobar degeneration (FTLD)-associated syndromes in Europe and found that they are more common than previously recognized, warranting diagnosis at any age. The findings have important implications for health and social care planning, as well as the design of future clinical trials.
Article
Neuroimaging
Leonie Lampe, Hans-Jurgen Huppertz, Sarah Anderl-Straub, Franziska Albrecht, Tommaso Ballarini, Sandrine Bisenius, Karsten Mueller, Sebastian Niehaus, Klaus Fassbender, Klaus Fliessbach, Holger Jahn, Johannes Kornhuber, Martin Lauer, Johannes Prudlo, Anja Schneider, Matthis Synofzik, Jan Kassubek, Adrian Danek, Arno Villringer, Janine Diehl-Schmid, Markus Otto, Matthias L. Schroeter
Summary: The study aimed to build a classifier for multiple dementia syndromes using MRI, and the results showed that the binary classification models achieved high prediction accuracies between 71% and 95%, with disease-specific atrophy patterns reflected in feature importance. The multi-syndrome model reached accuracies more than three times higher than chance level but was still far from 100%, and the performance varied across different dementia syndromes.
NEUROIMAGE-CLINICAL
(2023)
Article
Clinical Neurology
Patrick Oeckl, Marina Bluma, Marco Bucci, Steffen Halbgebauer, Konstantinos Chiotis, Anna Sandebring-Matton, Nicholas J. Ashton, Guglielmo Di Molfetta, Lana Grotschel, Miia Kivipelto, Kaj Blennow, Henrik Zetterberg, Irina Savitcheva, Agneta Nordberg, Markus Otto
Summary: Plasma beta-synuclein levels were found to be higher in individuals with Alzheimer's disease and mild cognitive impairment with amyloid-beta positivity. It demonstrated good discrimination and prediction of amyloid-beta status. The correlation between plasma beta-synuclein and amyloid-beta PET was observed in multiple cortical regions. These findings suggest that beta-synuclein is not a direct marker of amyloid-beta pathology and highlight the different longitudinal dynamics of synaptic degeneration and amyloid deposition in Alzheimer's disease.
ALZHEIMERS & DEMENTIA
(2023)
Article
Clinical Neurology
Patrick Oeckl, Shorena Janelidze, Steffen Halbgebauer, Erik Stomrud, Sebastian Palmqvist, Markus Otto, Oskar Hansson
Summary: Beta-synuclein can serve as a synaptic blood biomarker for Alzheimer's disease, even in the preclinical stages. Higher levels of plasma beta-synuclein are observed in preclinical AD, mild cognitive impairment, and AD dementia. The levels are associated with amyloid and tau pathology, as well as cognitive impairment and temporal cortical thinning.
ALZHEIMERS & DEMENTIA
(2023)
Article
Clinical Neurology
Babak Hooshmand, Franziska Appold, Patrick Fissler, Robert Perneczky, Markus Otto, Hayrettin Tumani, Miia Kivipelto, Christine A. F. von Arnim
Summary: This study investigated the association between vitamin B12 status and biomarkers of Alzheimer's disease. The results suggest that vitamin B12-related markers are associated with AD biomarkers and cognitive performance. Vitamin B12 supplementation may be important in slowing down brain changes and cognitive decline.
ANNALS OF NEUROLOGY
(2023)
Article
Neurosciences
Aurelie P. Bussy, Jake P. Levy, Tristin Best, Raihaan Patel, Lani Cupo, Tim Van Langenhove, Jorgen E. Nielsen, Yolande Pijnenburg, Maria Landqvist M. Waldo, Anne M. L. Remes, Matthias L. Schroeter, Isabel Santana, Florence Pasquier, Markus Otto, Adrian Danek, Johannes Levin, Isabelle Le Ber, Rik Vandenberghe, Matthis Synofzik, Fermin Moreno, Alexandre de Mendonca, Raquel Sanchez-Valle, Robert Laforce, Tobias Langheinrich, Alexander Gerhard, Caroline R. Graff, Chris R. Butler, Sandro Sorbi, Lize Jiskoot, Harro C. Seelaar, John C. van Swieten, Elizabeth Finger, Maria Carmela Tartaglia, Mario Masellis, Pietro Tiraboschi, Daniela Galimberti, Barbara B. Borroni, James B. Rowe, Martina D. Bocchetta, Jonathan D. A. Rohrer, Gabriel A. Devenyi, M. Mallar Chakravarty, Simon Ducharme
Summary: Recent studies have shown that early cerebellar and subcortical changes are seen in the progression of genetic frontotemporal dementia due to specific gene mutations. This study aimed to investigate the relationship between cerebellar and subcortical atrophy and neuropsychiatric symptoms across different genetic mutations in FTD.
HUMAN BRAIN MAPPING
(2023)
Article
Clinical Neurology
Anna Semenkova, Olivier Piguet, Andreas Johnen, Matthias L. Schroeter, Jannis Godulla, Christoph Linnemann, Markus Muhlhauser, Thomas Sauer, Markus Baumgartner, Sarah Anderl-Straub, Markus Otto, Ansgar Felbecker, Reto W. Kressig, Manfred Berres, Marc Sollberger
Summary: Based on the revised diagnostic criteria, the Behavioural Dysfunction Questionnaire (BDQ) was developed to discriminate between behavioural variant frontotemporal dementia (bvFTD) and non-bvFTD patients. BDQ-scores without any time criterion were found highly discriminatory between early-stage bvFTD and non-bvFTD groups, suggesting its potential for improving early diagnosis of bvFTD.
JOURNAL OF NEUROLOGY
(2023)
Article
Clinical Neurology
Ahmed Abdelhak, Lorenzo Barba, Michele Romoli, Pascal Benkert, Francesco Conversi, Lucio D'Anna, Ruturaj R. Masvekar, Bibiana Bielekova, Mercedes Prudencio, Leonard Petrucelli, James F. Meschia, Young Erben, Roberto Furlan, Rebecca De Lorenzo, Alessandra Mandelli, Raoul Sutter, Lisa Hert, Varenka Epple, Damiano Marastoni, Johann Sellner, Petra Steinacker, Anne Hege Aamodt, Lars Heggelund, Anne Margarita Dyrhol-Riise, Johan Virhammar, David Fallmar, Elham Rostami, Eva Kumlien, Kaj Blennow, Henrik Zetterberg, Hayrettin Tumani, Simona Sacco, Ari J. Green, Markus Otto, Jens Kuhle, Raffaele Ornello, Matteo Foschi, Samir Abu-Rumeileh
Summary: This study investigated the prognostic value of blood neurofilament light chain protein (NfL) levels in the acute phase of COVID-19. The results showed that elevated NfL levels were significantly associated with disease duration and severity, and higher NfL levels were linked to a higher likelihood of ICU admission, need of mechanical ventilation, and death. Therefore, blood NfL levels may have potential in predicting the prognosis of COVID-19.
JOURNAL OF NEUROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Badrieh Fazeli, Andre Huss, Nerea Gomez de San Jose, Markus Otto, Hayrettin Tumani, Steffen Halbgebauer
Summary: This study developed a sensitive and easy-to-use microfluidic assay to measure GFAP in blood, and confirmed previous findings of elevated GFAP levels in AD by applying the assay in a cohort of clinically characterized patients.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Clinical Neurology
Martina Bocchetta, Emily G. Todd, Arabella Bouzigues, David M. Cash, Jennifer M. Nicholas, Rhian S. Convery, Lucy L. Russell, David L. Thomas, Ian B. Malone, Juan Eugenio Iglesias, John C. van Swieten, Lize C. Jiskoot, Harro Seelaar, Barbara Borroni, Daniela Galimberti, Raquel Sanchez-Valle, Robert Laforce, Fermin Moreno, Matthis Synofzik, Caroline Graff, Mario Masellis, Maria Carmela Tartaglia, James B. Rowe, Rik Vandenberghe, Elizabeth Finger, Fabrizio Tagliavini, Alexandre de Mendonca, Isabel Santana, Chris R. Butler, Simon Ducharme, Alexander Gerhard, Adrian Danek, Johannes Levin, Markus Otto, Sandro Sorbi, Isabelle Le Ber, Florence Pasquier, Jonathan D. Rohrer
Summary: The study quantified brain anomalies on MRI in individuals with C9orf72, MAPT, and GRN mutations. The identified imaging markers associated with clinical and behavioral changes in presymptomatic carriers over one year, providing important data for participant stratification in trials. Biomarkers predicting disease progression in genetic frontotemporal dementia are urgently needed.
BRAIN COMMUNICATIONS
(2023)
Editorial Material
Neurosciences
Lorenzo Barba, Markus Otto, Samir Abu-Rumeileh
Summary: Around 10-15% of amyotrophic lateral sclerosis (ALS) cases show concurrent Alzheimer's disease (AD) pathology. These ALS-AD patients have a higher prevalence of amnestic cognitive disturbances before motor symptoms appear. While cerebrospinal fluid (CSF) AD core biomarkers do not show significant changes in ALS, phosphorylated tau protein levels in blood may increase independently of AD copathology. Neurofilament proteins are consistently elevated in ALS, but there is limited research on their role in ALS-AD. These considerations are important when using fluid biomarkers as inclusion criteria or secondary endpoints in clinical trials.
JOURNAL OF ALZHEIMERS DISEASE
(2023)
Article
Clinical Neurology
Samir Abu-Rumeileh, Steffen Halbgebauer, Giuseppe Mario Bentivenga, Lorenzo Barba, Simone Baiardi, Andrea Mastrangelo, Patrick Oeckl, Petra Steinacker, Angela Mammana, Sabina Capellari, Markus Otto, Piero Parchi
Summary: Beta-synuclein is a promising biomarker for synaptic damage in cerebrospinal fluid and blood, and it can accurately distinguish sporadic Creutzfeldt-Jakob disease from non-prion rapidly progressive dementias. It outperforms protein 14-3-3 and total tau in cerebrospinal fluid, and has similar diagnostic value as cerebrospinal fluid biomarkers in plasma. Beta-synuclein may serve as the first highly accurate blood biomarker for the diagnosis of sporadic Creutzfeldt-Jakob disease.
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2023)
Article
Clinical Neurology
Samir Abu-Rumeileh, Lorenzo Barba, Matthias Bache, Steffen Halbgebauer, Patrick Oeckl, Petra Steinacker, Antje Guettler, Jacqueline Kessler, Joerg Illert, Christian Strauss, Dirk Vordermark, Markus Otto
Summary: The concentrations and prognostic roles of plasma β-synuclein, glial fibrillary acidic protein (GFAP), and neurofilament proteins (NfL and NfH) were analyzed in 33 patients with malignant gliomas. GFAP and NfL levels were higher in glioblastoma patients compared to other tumor cases. Post-surgery, β-syn, NfL, and NfH increased, while GFAP decreased in long-term follow-up. Surgery and/or radiotherapy regimens influenced β-syn and neurofilament concentrations. GFAP and neurofilament levels were significantly associated with survival. Plasma neuronal and astrocytic biomarkers are differentially altered in malignant glioma types and have distinct trajectories after surgical and adjuvant therapy.
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2023)