Re-introducing non-optimal synonymous codons into codon-optimized constructs enhances soluble recovery of recombinant proteins from Escherichia coli
Published 2019 View Full Article
- Home
- Publications
- Publication Search
- Publication Details
Title
Re-introducing non-optimal synonymous codons into codon-optimized constructs enhances soluble recovery of recombinant proteins from Escherichia coli
Authors
Keywords
Guanosine triphosphatase, Solubility, Cloning, Protein translation, Ribosomes, Transfer RNA, Centrifugation, Recombinant proteins
Journal
PLoS One
Volume 14, Issue 4, Pages e0215892
Publisher
Public Library of Science (PLoS)
Online
2019-04-24
DOI
10.1371/journal.pone.0215892
References
Ask authors/readers for more resources
Related references
Note: Only part of the references are listed.- High-throughput evaluation of T7 promoter variants using biased randomization and DNA barcoding
- (2018) Ryo Komura et al. PLoS One
- Measurements of translation initiation from all 64 codons in E. coli
- (2017) Ariel Hecht et al. NUCLEIC ACIDS RESEARCH
- A fully automated procedure for the parallel, multidimensional purification and nucleotide loading of the human GTPases KRas, Rac1 and RalB
- (2017) Christopher H. Gray et al. PROTEIN EXPRESSION AND PURIFICATION
- Streamlining workflow and automation to accelerate laboratory scale protein production
- (2017) Jennifer Konczal et al. PROTEIN EXPRESSION AND PURIFICATION
- %MinMax: A versatile tool for calculating and comparing synonymous codon usage and its impact on protein folding
- (2017) Anabel Rodriguez et al. PROTEIN SCIENCE
- Tunable recombinant protein expression in E. coli: promoter systems and genetic constraints
- (2016) Lukas Marschall et al. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
- Quality over quantity: optimizing co-translational protein folding with non-‘optimal’ synonymous codons
- (2016) Giselle N Jacobson et al. CURRENT OPINION IN STRUCTURAL BIOLOGY
- The Effect of an Alternate Start Codon on Heterologous Expression of a PhoA Fusion Protein in Mycoplasma gallisepticum
- (2015) Indu S. Panicker et al. PLoS One
- Synonymous codons, ribosome speed, and eukaryotic gene expression regulation
- (2014) Daniel Tarrant et al. CELLULAR AND MOLECULAR LIFE SCIENCES
- A critical analysis of codon optimization in human therapeutics
- (2014) Vincent P. Mauro et al. TRENDS IN MOLECULAR MEDICINE
- Expression of codon optimized genes in microbial systems: current industrial applications and perspectives
- (2014) Claudia Elena et al. Frontiers in Microbiology
- Gene Position More Strongly Influences Cell-Free Protein Expression from Operons than T7 Transcriptional Promoter Strength
- (2013) Fabio Chizzolini et al. ACS Synthetic Biology
- tRNA concentration fine tunes protein solubility
- (2012) Ivan Fedyunin et al. FEBS LETTERS
- Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution
- (2012) Siddhartha Paul et al. NUCLEIC ACIDS RESEARCH
- Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: Advantages of high-throughput screening
- (2011) Agustín Correa et al. Biotechnology Journal
- Increased incidence of rare codon clusters at 5' and 3' gene termini:implications for function
- (2010) Thomas F Clarke et al. BMC GENOMICS
- Advancing high-throughput gene synthesis technology
- (2009) Jingdong Tian et al. Molecular BioSystems
- Tuning Escherichia coli for membrane protein overexpression
- (2008) S. Wagner et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Publish scientific posters with Peeref
Peeref publishes scientific posters from all research disciplines. Our Diamond Open Access policy means free access to content and no publication fees for authors.
Learn MoreCreate your own webinar
Interested in hosting your own webinar? Check the schedule and propose your idea to the Peeref Content Team.
Create Now