Trypanosoma brucei ribonuclease H2A is an essential R-loop processing enzyme whose loss causes DNA damage during transcription initiation and antigenic variation

Programmed DNA Breaks Activate the Germline Genome in Caenorhabditis elegans

(2018) Matthew M. Wong et al.   DEVELOPMENTAL CELL

Genome-wide Map of R-Loop-Induced Damage Reveals How a Subset of R-Loops Contributes to Genomic Instability

(2018) Lorenzo Costantino et al.   MOLECULAR CELL

Genome-wide mapping reveals conserved and diverged R-loop activities in the unusual genetic landscape of the African trypanosome genome

(2018) Emma Briggs et al.   NUCLEIC ACIDS RESEARCH

Ribonuclease H1-targeted R-loops in surface antigen gene expression sites can direct trypanosome immune evasion

(2018) Emma Briggs et al.   PLoS Genetics

Canonical DNA Repair Pathways Influence R-Loop-Driven Genome Instability

(2017) Peter C. Stirling et al.   JOURNAL OF MOLECULAR BIOLOGY

Rad52 Inverse Strand Exchange Drives RNA-Templated DNA Double-Strand Break Repair

(2017) Olga M. Mazina et al.   MOLECULAR CELL

DNA–RNA hybrids: the risks of DNA breakage during transcription

(2017) Andrés Aguilera et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Trypanosoma brucei RAP1 maintains telomere and subtelomere integrity by suppressing TERRA and telomeric RNA:DNA hybrids

(2017) Vishal Nanavaty et al.   NUCLEIC ACIDS RESEARCH

DNA–RNA hybrids: the risks of DNA breakage during transcription

(2017) Andrés Aguilera et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Genome-wide and protein kinase-focused RNAi screens reveal conserved and novel damage response pathways in Trypanosoma brucei

(2017) Jennifer A. Stortz et al.   PLoS Pathogens

High-resolution analysis of multi-copy variant surface glycoprotein gene expression sites in African trypanosomes

(2016) Sebastian Hutchinson et al.   BMC GENOMICS

Creative damage unleashes transcription

(2016) Stuart K. Calderwood   CELL CYCLE

Does DNA replication direct locus-specific recombination during host immune evasion by antigenic variation in the African trypanosome?

(2016) Rebecca Devlin et al.   CURRENT GENETICS

Gene expression in Kinetoplastids

(2016) CE Clayton   CURRENT OPINION IN MICROBIOLOGY

cGAS‐STING do it again: pivotal role in RNase H2 genetic disease

(2016) Matteo Gentili et al.   EMBO JOURNAL

Ribonuclease H2 mutations induce a cGAS/STING‐dependent innate immune response

(2016) Karen J Mackenzie et al.   EMBO JOURNAL

Role of genome guardian proteins in transcriptional elongation

(2016) Heeyoun Bunch   FEBS LETTERS

RNA polymerase II promoter-proximal pausing in mammalian long non-coding genes

(2016) Heeyoun Bunch et al.   GENOMICS

RNase H2 catalytic core Aicardi-Goutières syndrome–related mutant invokes cGAS–STING innate immune-sensing pathway in mice

(2016) Vladislav Pokatayev et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Prevalent, Dynamic, and Conserved R-Loop Structures Associate with Specific Epigenomic Signatures in Mammals

(2016) Lionel A. Sanz et al.   MOLECULAR CELL

VEX1 controls the allelic exclusion required for antigenic variation in trypanosomes

(2016) Lucy Glover et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Balancing Act of Ribonucleotides in DNA

(2016) Susana M. Cerritelli et al.   TRENDS IN BIOCHEMICAL SCIENCES

Mapping replication dynamics in Trypanosoma brucei reveals a link with telomere transcription and antigenic variation

(2016) Rebecca Devlin et al.   eLife

Characterization of human disease phenotypes associated with mutations inTREX1,RNASEH2A,RNASEH2B,RNASEH2C,SAMHD1,ADAR, andIFIH1

(2015) Yanick J. Crow et al.   AMERICAN JOURNAL OF MEDICAL GENETICS PART A

The Yin and Yang of R-loop biology

(2015) Lorenzo Costantino et al.   CURRENT OPINION IN CELL BIOLOGY

Stimulation of Chromosomal Rearrangements by Ribonucleotides

(2015) H. N. Conover et al.   GENETICS

Elevated Genome-Wide Instability in Yeast Mutants Lacking RNase H Activity

(2015) K. O'Connell et al.   GENETICS

Architecture of a Host–Parasite Interface: Complex Targeting Mechanisms Revealed Through Proteomics

(2015) Catarina Gadelha et al.   MOLECULAR & CELLULAR PROTEOMICS

The Overlooked Fact: Fundamental Need for Spike-In Control for Virtually All Genome-Wide Analyses

(2015) Kaifu Chen et al.   MOLECULAR AND CELLULAR BIOLOGY

R loops: new modulators of genome dynamics and function

(2015) José M. Santos-Pereira et al.   NATURE REVIEWS GENETICS

Breaking bad: R-loops and genome integrity

(2015) Julie Sollier et al.   TRENDS IN CELL BIOLOGY

Transcriptional elongation requires DNA break-induced signalling

(2015) Heeyoun Bunch et al.   Nature Communications

R-loops and initiation of DNA replication in human cells: a missing link?

(2015) Rodrigo Lombraña et al.   Frontiers in Genetics

Innate Immune Sensing and Signaling of Cytosolic Nucleic Acids

(2014) Jiaxi Wu et al.   Annual Review of Immunology

A double-edged sword: R loops as threats to genome integrity and powerful regulators of gene expression

(2014) K. Skourti-Stathaki et al.   GENES & DEVELOPMENT

Capturing the variant surface glycoprotein repertoire (the VSGnome) of Trypanosoma brucei Lister 427

(2014) George A.M. Cross et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Transcription-Coupled Nucleotide Excision Repair Factors Promote R-Loop-Induced Genome Instability

(2014) Julie Sollier et al.   MOLECULAR CELL

Nucleotide excision repair inTrypanosoma brucei: specialization of transcription-coupled repair due to multigenic transcription

(2014) Carlos R. Machado et al.   MOLECULAR MICROBIOLOGY

Transcript-RNA-templated DNA recombination and repair

(2014) Havva Keskin et al.   NATURE

deepTools: a flexible platform for exploring deep-sequencing data

(2014) Fidel Ramírez et al.   NUCLEIC ACIDS RESEARCH

Regulators of Trypanosoma brucei Cell Cycle Progression and Differentiation Identified Using a Kinome-Wide RNAi Screen

(2014) Nathaniel G. Jones et al.   PLoS Pathogens

Antigenic variation in African trypanosomes: the importance of chromosomal and nuclear context inVSGexpression control

(2013) Lucy Glover et al.   CELLULAR MICROBIOLOGY

Aicardi-Goutières syndrome: a model disease for systemic autoimmunity

(2013) M. A. Lee-Kirsch et al.   CLINICAL AND EXPERIMENTAL IMMUNOLOGY

The homologous recombination machinery modulates the formation of RNA–DNA hybrids and associated chromosome instability

(2013) Lamia Wahba et al.   eLife

Enzymatic Removal of Ribonucleotides from DNA Is Essential for Mammalian Genome Integrity and Development

(2012) Martin A.M. Reijns et al.   CELL

Ribonucleotide incorporation, proofreading and bypass by human DNA polymerase δ

(2012) Anders R. Clausen et al.   DNA REPAIR

R-loop-mediated genome instability in mRNA cleavage and polyadenylation mutants

(2012) P. C. Stirling et al.   GENES & DEVELOPMENT

Trypanosomes lacking uracil-DNA glycosylase are hypersensitive to antifolates and present a mutator phenotype

(2012) Víctor M. Castillo-Acosta et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Human DNA Polymerase ϵ Is Able to Efficiently Extend from Multiple Consecutive Ribonucleotides

(2012) A. Yasemin Göksenin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mammalian RNase H2 removes ribonucleotides from DNA to maintain genome integrity

(2012) Bjoern Hiller et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Trypanosomal histone γH2A and the DNA damage response

(2012) Lucy Glover et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

RNase H2-Initiated Ribonucleotide Excision Repair

(2012) Justin L. Sparks et al.   MOLECULAR CELL

R-Loop Formation Is a Distinctive Characteristic of Unmethylated Human CpG Island Promoters

(2012) Paul A. Ginno et al.   MOLECULAR CELL

R Loops: From Transcription Byproducts to Threats to Genome Stability

(2012) Andrés Aguilera et al.   MOLECULAR CELL

Genome-wide Analysis Reveals Extensive Functional Interaction between DNA Replication Initiation and Transcription in the Genome of Trypanosoma brucei

(2012) Calvin Tiengwe et al.   Cell Reports

Unlocking the Sugar “Steric Gate” of DNA Polymerases

(2011) Jessica A. Brown et al.   BIOCHEMISTRY

RNase H and Multiple RNA Biogenesis Factors Cooperate to Prevent RNA:DNA Hybrids from Generating Genome Instability

(2011) Lamia Wahba et al.   MOLECULAR CELL

PCNA directs type 2 RNase H activity on DNA replication and repair substrates

(2011) Doryen Bubeck et al.   NUCLEIC ACIDS RESEARCH

Gene expression in Trypanosoma brucei: lessons from high-throughput RNA sequencing

(2011) T. Nicolai Siegel et al.   TRENDS IN PARASITOLOGY

Histone H3 trimethylated at lysine 4 is enriched at probable transcription start sites in Trypanosoma brucei

(2010) Jessica R. Wright et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Two thymidine hydroxylases differentially regulate the formation of glucosylated DNA at regions flanking polymerase II polycistronic transcription units throughout the genome of Trypanosoma brucei

(2010) Laura J. Cliffe et al.   NUCLEIC ACIDS RESEARCH

Abundant ribonucleotide incorporation into DNA by yeast replicative polymerases

(2010) S. A. Nick McElhinny et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Transcriptome of the Human Pathogen Trypanosoma brucei at Single-Nucleotide Resolution

(2010) Nikolay G. Kolev et al.   PLoS Pathogens

Ribonuclease H: the enzymes in eukaryotes

(2009) Susana M. Cerritelli et al.   FEBS Journal

Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes

(2009) Takashi Tadokoro et al.   FEBS Journal

Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma brucei

(2009) T. N. Siegel et al.   GENES & DEVELOPMENT

Competition between the RNA Transcript and the Nontemplate DNA Strand during R-Loop Formation In Vitro: a Nick Can Serve as a Strong R-Loop Initiation Site

(2009) D. Roy et al.   MOLECULAR AND CELLULAR BIOLOGY

Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei

(2008) Sam Alsford et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Telomeric Expression Sites Are Highly Conserved in Trypanosoma brucei

(2008) Christiane Hertz-Fowler et al.   PLoS One