Oleoresin defenses in conifers: chemical diversity, terpene synthases and limitations of oleoresin defense under climate change

The cytochrome P450 CYP6DE1 catalyzes the conversion of α-pinene into the mountain pine beetle aggregation pheromone trans-verbenol

(2019) Christine C. Chiu et al.   Scientific Reports

Multiyear weather anomalies associated with range shifts by the mountain pine beetle preceding large epidemics

(2019) Kishan R. Sambaraju et al.   FOREST ECOLOGY AND MANAGEMENT

Cytochrome P450 enzymes: A driving force of plant diterpene diversity

(2019) Ulschan Bathe et al.   PHYTOCHEMISTRY

Functions of mountain pine beetle cytochromes P450 CYP6DJ1, CYP6BW1 and CYP6BW3 in the oxidation of pine monoterpenes and diterpene resin acids

(2019) Christine C. Chiu et al.   PLoS One

Isolation, characterization and functional analysis of a novel 3-hydroxy-3-methylglutaryl-coenzyme A synthase gene (GbHMGS2) from Ginkgo biloba

(2018) Xiangxiang Meng et al.   ACTA PHYSIOLOGIAE PLANTARUM

Monoterpenyl esters in juvenile mountain pine beetle and sex-specific release of the aggregation pheromonetrans-verbenol

(2018) Christine C. Chiu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An annotated transcriptome of highly inbred Thuja plicata (Cupressaceae) and its utility for gene discovery of terpenoid biosynthesis and conifer defense

(2018) Tal J. Shalev et al.   Tree Genetics & Genomes

Cytochromes P450 Preferentially Expressed in Antennae of the Mountain Pine Beetle

(2018) Christine C. Chiu et al.   JOURNAL OF CHEMICAL ECOLOGY

Functions of stone cells and oleoresin terpenes in the conifer defense syndrome

(2018) Justin G. A. Whitehill et al.   NEW PHYTOLOGIST

Characterization, Function, and Transcriptional Profiling Analysis of 3-Hydroxy-3-methylglutaryl-CoA Synthase Gene (GbHMGS1) towards Stresses and Exogenous Hormone Treatments in Ginkgo biloba

(2017) et al.   MOLECULES

Defence syndromes in lodgepole - whitebark pine ecosystems relate to degree of historical exposure to mountain pine beetles

(2017) Kenneth F. Raffa et al.   PLANT CELL AND ENVIRONMENT

Cell-type- and tissue-specific transcriptomes of the white spruce (Picea glauca ) bark unmask fine-scale spatial patterns of constitutive and induced conifer defense

(2017) Jose M. Celedon et al.   PLANT JOURNAL

Biosynthesis of the microtubule-destabilizing diterpene pseudolaric acid B from golden larch involves an unusual diterpene synthase

(2017) Sibongile Mafu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Transcriptome Assembly and Systematic Identification of Novel Cytochrome P450s in Taxus chinensis

(2017) Weifang Liao et al.   Frontiers in Plant Science

LOCALIZER: subcellular localization prediction of both plant and effector proteins in the plant cell

(2017) Jana Sperschneider et al.   Scientific Reports

Toxicity of Pine Monoterpenes to Mountain Pine Beetle

(2017) Christine C. Chiu et al.   Scientific Reports

Climate influences on whitebark pine mortality from mountain pine beetle in the Greater Yellowstone Ecosystem

(2016) Polly C. Buotte et al.   ECOLOGICAL APPLICATIONS

The influence of variation in host tree monoterpene composition on secondary attraction by an invasive bark beetle: Implications for range expansion and potential host shift by the mountain pine beetle

(2016) Jordan L. Burke et al.   FOREST ECOLOGY AND MANAGEMENT

Sequence of the Sugar Pine Megagenome

(2016) Kristian A. Stevens et al.   GENETICS

Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees

(2016) Ivica Letunic et al.   NUCLEIC ACIDS RESEARCH

Modularity of Conifer Diterpene Resin Acid Biosynthesis: P450 Enzymes of Different CYP720B Clades Use Alternative Substrates and Converge on the Same Products

(2016) Katrin Geisler et al.   PLANT PHYSIOLOGY

Sequence of the Sugar Pine Megagenome

(2016) Kristian A. Stevens et al.   GENETICS

Tree mortality from drought, insects, and their interactions in a changing climate

(2015) William R. L. Anderegg et al.   NEW PHYTOLOGIST

Improved white spruce (Picea glauca) genome assemblies and annotation of large gene families of conifer terpenoid and phenolic defense metabolism

(2015) René L. Warren et al.   PLANT JOURNAL

The Gymnosperm Cytochrome P450 CYP750B1 Catalyzes Stereospecific Monoterpene Hydroxylation of (+)-Sabinene in Thujone Biosynthesis in Western Redcedar

(2015) Andreas Gesell et al.   PLANT PHYSIOLOGY

Orthologs of the archaeal isopentenyl phosphate kinase regulate terpenoid production in plants

(2015) Laura K. Henry et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plant diterpene synthases: exploring modularity and metabolic diversity for bioengineering

(2015) Philipp Zerbe et al.   TRENDS IN BIOTECHNOLOGY

Gene Discovery for Enzymes Involved in Limonene Modification or Utilization by the Mountain Pine Beetle-Associated Pathogen Grosmannia clavigera

(2014) Ye Wang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Valencene oxidase CYP706M1 from Alaska cedar (Callitropsis nootkatensis)

(2014) Katarina Cankar et al.   FEBS LETTERS

Unique Features of the Loblolly Pine (Pinus taeda L.) Megagenome Revealed Through Sequence Annotation

(2014) J. L. Wegrzyn et al.   GENETICS

Sequencing and Assembly of the 22-Gb Loblolly Pine Genome

(2014) A. Zimin et al.   GENETICS

Plasticity and Evolution of (+)-3-Carene Synthase and (−)-Sabinene Synthase Functions of a Sitka Spruce Monoterpene Synthase Gene Family Associated with Weevil Resistance

(2014) Christopher R. Roach et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Observations and models of emissions of volatile terpenoid compounds from needles of ponderosa pine trees growing in situ: control by light, temperature and stomatal conductance

(2014) Peter Harley et al.   OECOLOGIA

Decoding the massive genome of loblolly pine using haploid DNA and novel assembly strategies

(2014) David B Neale et al.   GENOME BIOLOGY

Comparison of lodgepole and jack pine resin chemistry: implications for range expansion by the mountain pine beetle,Dendroctonus ponderosae(Coleoptera: Curculionidae)

(2014) Erin L. Clark et al.   PeerJ

Network Analysis of the MVA and MEP Pathways for Isoprenoid Synthesis

(2013) Eva Vranová et al.   Annual Review of Plant Biology

Mountain Pine Beetles Colonizing Historical and Naïve Host Trees Are Associated with a Bacterial Community Highly Enriched in Genes Contributing to Terpene Metabolism

(2013) Aaron S. Adams et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Assembling the 20 Gb white spruce (Picea glauca) genome from whole-genome shotgun sequencing data

(2013) Inanc Birol et al.   BIOINFORMATICS

Transcriptome resources and functional characterization of monoterpene synthases for two host species of the mountain pine beetle, lodgepole pine (Pinus contorta) and jack pine (Pinus banksiana)

(2013) Dawn E Hall et al.   BMC PLANT BIOLOGY

Terpenes Tell Different Tales at Different Scales: Glimpses into the Chemical Ecology of Conifer - Bark Beetle - Microbial Interactions

(2013) Kenneth F. Raffa   JOURNAL OF CHEMICAL ECOLOGY

Bacteria Associated with a Tree-Killing Insect Reduce Concentrations of Plant Defense Compounds

(2013) Celia K. Boone et al.   JOURNAL OF CHEMICAL ECOLOGY

The Norway spruce genome sequence and conifer genome evolution

(2013) Björn Nystedt et al.   NATURE

Chemical similarity between historical and novel host plants promotes range and host expansion of the mountain pine beetle in a naïve host ecosystem

(2013) Nadir Erbilgin et al.   NEW PHYTOLOGIST

Valencene synthase from the heartwood of Nootka cypress (Callitropsis nootkatensis) for biotechnological production of valencene

(2013) Jules Beekwilder et al.   PLANT BIOTECHNOLOGY JOURNAL

Identification of Genes in Thuja plicata Foliar Terpenoid Defenses

(2013) A. J. Foster et al.   PLANT PHYSIOLOGY

Gene Discovery of Modular Diterpene Metabolism in Nonmodel Systems

(2013) P. Zerbe et al.   PLANT PHYSIOLOGY

Deep Sequencing Reveals Transcriptome Re-Programming of Taxus × media Cells to the Elicitation with Methyl Jasmonate

(2013) Guiling Sun et al.   PLoS One

Temperature-driven range expansion of an irruptive insect heightened by weakly coevolved plant defenses

(2013) K. F. Raffa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Draft genome of the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major forest pest

(2013) Christopher I Keeling et al.   GENOME BIOLOGY

Transcriptional profile of Taxus chinensis cells in response to methyl jasmonate

(2012) Shu-tao Li et al.   BMC GENOMICS

ISOPRENE SYNTHASE GENES FORM A MONOPHYLETIC CLADE OF ACYCLIC TERPENE SYNTHASES IN THE TPS-B TERPENE SYNTHASE FAMILY

(2012) Thomas D. Sharkey et al.   EVOLUTION

Insights into Diterpene Cyclization from Structure of Bifunctional Abietadiene Synthase fromAbies grandis

(2012) Ke Zhou et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Bifunctionalcis-Abienol Synthase fromAbies balsameaDiscovered by Transcriptome Sequencing and Its Implications for Diterpenoid Fragrance Production

(2012) Philipp Zerbe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Terpenoid synthase structures: a so far incomplete view of complex catalysis

(2012) Yang Gao et al.   NATURAL PRODUCT REPORTS

Microbial engineering for the production of advanced biofuels

(2012) Pamela P. Peralta-Yahya et al.   NATURE

A specialized ABC efflux transporter GcABC-G1 confers monoterpene resistance toGrosmannia clavigera, a bark beetle-associated fungal pathogen of pine trees

(2012) Ye Wang et al.   NEW PHYTOLOGIST

Inducibility of chemical defenses in Norway spruce bark is correlated with unsuccessful mass attacks by the spruce bark beetle

(2012) Christian Schiebe et al.   OECOLOGIA

Evolution of Conifer Diterpene Synthases: Diterpene Resin Acid Biosynthesis in Lodgepole Pine and Jack Pine Involves Monofunctional and Bifunctional Diterpene Synthases

(2012) D. E. Hall et al.   PLANT PHYSIOLOGY

Transcriptome mining, functional characterization, and phylogeny of a large terpene synthase gene family in spruce (Picea spp.)

(2011) Christopher I Keeling et al.   BMC PLANT BIOLOGY

Efficacy of tree defense physiology varies with bark beetle population density: a basis for positive feedback in eruptive species

(2011) Celia K. Boone et al.   CANADIAN JOURNAL OF FOREST RESEARCH

Biochemical Characterization and Homology Modeling of Methylbutenol Synthase and Implications for Understanding Hemiterpene Synthase Evolution in Plants

(2011) Dennis W. Gray et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Primary Diterpene Synthase Products ofPicea abiesLevopimaradiene/Abietadiene Synthase (PaLAS) Are Epimers of a Thermally Unstable Diterpenol

(2011) Christopher I. Keeling et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

UCSF Chimera, MODELLER, and IMP: An integrated modeling system

(2011) Zheng Yang et al.   JOURNAL OF STRUCTURAL BIOLOGY

Mountain pine beetle host-range expansion threatens the boreal forest

(2011) CATHERINE I. CULLINGHAM et al.   MOLECULAR ECOLOGY

Mutational analysis of white spruce (Picea glauca) ent-kaurene synthase (PgKS) reveals common and distinct mechanisms of conifer diterpene synthases of general and specialized metabolism

(2011) Philipp Zerbe et al.   PHYTOCHEMISTRY

The family of terpene synthases in plants: a mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom

(2011) Feng Chen et al.   PLANT JOURNAL

Induction of isoprenyl diphosphate synthases, plant hormones and defense signalling genes correlates with traumatic resin duct formation in Norway spruce (Picea abies)

(2011) Axel Schmidt et al.   PLANT MOLECULAR BIOLOGY

Evolution of Diterpene Metabolism: Sitka Spruce CYP720B4 Catalyzes Multiple Oxidations in Resin Acid Biosynthesis of Conifer Defense against Insects

(2011) B. Hamberger et al.   PLANT PHYSIOLOGY

The First Insight into the Tissue Specific Taxus Transcriptome via Illumina Second Generation Sequencing

(2011) Da Cheng Hao et al.   PLoS One

Genome and transcriptome analyses of the mountain pine beetle-fungal symbiont Grosmannia clavigera, a lodgepole pine pathogen

(2011) S. DiGuistini et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of a Three-Domain Sesquiterpene Synthase: A Prospective Target for Advanced Biofuels Production

(2011) Ryan P. McAndrew et al.   STRUCTURE

Identification and microbial production of a terpene-based advanced biofuel

(2011) Pamela P. Peralta-Yahya et al.   Nature Communications

Laser microdissection of conifer stem tissues: Isolation and analysis of high quality RNA, terpene synthase enzyme activity and terpenoid metabolites from resin ducts and cambial zone tissue of white spruce (Picea glauca)

(2010) Eric Abbott et al.   BMC PLANT BIOLOGY

Terpenoid metabolite profiling in Sitka spruce identifies association of dehydroabietic acid, (+)-3-carene, and terpinolene with resistance against white pine weevil

(2010) Jeanne A. Robert et al.   Botany

Terpenoid Biosynthesis and Specialized Vascular Cells of Conifer Defense

(2010) Katherine G. Zulak et al.   Journal of Integrative Plant Biology

Taxadiene synthase structure and evolution of modular architecture in terpene biosynthesis

(2010) Mustafa Köksal et al.   NATURE

3V: cavity, channel and cleft volume calculator and extractor

(2010) N. R. Voss et al.   NUCLEIC ACIDS RESEARCH

Species-specific responses of pine sesquiterpene synthases to sawfly oviposition

(2010) Diana Köpke et al.   PHYTOCHEMISTRY

Immunofluorescence localization of levopimaradiene/abietadiene synthase in methyl jasmonate treated stems of Sitka spruce (Picea sitchensis) shows activation of diterpenoid biosynthesis in cortical and developing traumatic resin ducts

(2010) Katherine G. Zulak et al.   PHYTOCHEMISTRY

An integrated genomic, proteomic and biochemical analysis of (+)-3-carene biosynthesis in Sitka spruce (Picea sitchensis) genotypes that are resistant or susceptible to white pine weevil

(2010) Dawn E. Hall et al.   PLANT JOURNAL

Identification and Functional Characterization of Monofunctional ent-Copalyl Diphosphate and ent-Kaurene Synthases in White Spruce Reveal Different Patterns for Diterpene Synthase Evolution for Primary and Secondary Metabolism in Gymnosperms

(2010) C. I. Keeling et al.   PLANT PHYSIOLOGY

Transcriptome Analysis ofTaxus cuspidataNeedles Based on 454 Pyrosequencing

(2010) Qiong Wu et al.   PLANTA MEDICA

Abiotic stresses and induced BVOCs

(2010) Francesco Loreto et al.   TRENDS IN PLANT SCIENCE

Characterization of 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase (HDS) gene from Ginkgo biloba

(2009) Sang-Min Kim et al.   MOLECULAR BIOLOGY REPORTS

Protein structure prediction on the Web: a case study using the Phyre server

(2009) Lawrence A Kelley et al.   Nature Protocols

Targeted proteomics using selected reaction monitoring reveals the induction of specific terpene synthases in a multi-level study of methyl jasmonate-treated Norway spruce (Picea abies)

(2009) Katherine G. Zulak et al.   PLANT JOURNAL

A Bifunctional Geranyl and Geranylgeranyl Diphosphate Synthase Is Involved in Terpene Oleoresin Formation in Picea abies

(2009) A. Schmidt et al.   PLANT PHYSIOLOGY

Regulation of resin acid synthesis in Pinus densiflora by differential transcription of genes encoding multiple 1-deoxy-d-xylulose 5-phosphate synthase and 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase genes

(2009) Yeon-Bok Kim et al.   TREE PHYSIOLOGY

Cross-scale Drivers of Natural Disturbances Prone to Anthropogenic Amplification: The Dynamics of Bark Beetle Eruptions

(2008) Kenneth F. Raffa et al.   BIOSCIENCE

A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464 high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea sitchensis)

(2008) Steven G Ralph et al.   BMC GENOMICS

Movement of outbreak populations of mountain pine beetle: influences of spatiotemporal patterns and climate

(2008) Brian H. Aukema et al.   ECOGRAPHY

Mountain pine beetle and forest carbon feedback to climate change

(2008) W. A. Kurz et al.   NATURE

Phylogeny.fr: robust phylogenetic analysis for the non-specialist

(2008) A. Dereeper et al.   NUCLEIC ACIDS RESEARCH

Does egg deposition by herbivorous pine sawflies affect transcription of sesquiterpene synthases in pine?

(2008) Diana Köpke et al.   PLANTA

Functional plasticity of paralogous diterpene synthases involved in conifer defense

(2008) C. I. Keeling et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cloning and characterization of two different types of geranyl diphosphate synthases from Norway spruce (Picea abies)

(2007) Axel Schmidt et al.   PHYTOCHEMISTRY