Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 21, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.nano.2019.102073
Keywords
Nanoparticles; PLGA NP; Chitosan-PLGA NP; PBMC; Monocyte-derived DC; Intracellular trafficking
Funding
- Helmholtz Zukunftsthema Immunology und Inflammation [ZT-0027]
- Deutsche Forschungsgemeinschaft (DFG, Joint French-German Project cGAS-VAV) [406922110]
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Biodegradable polymeric nanoparticles (NP) made from poly (lactid-co-glycolide) acid (PLGA) and chitosan (CS) hold promise as innovative formulations for targeted delivery. Since interactions of such NP with primary human immune cells have not been characterized, yet, here we assessed the effect of PLGA or CS-PLGA NP treatment on human peripheral blood mononuclear cells (PBMC), as well as on monocyte-derived DC (moDC). Amongst PBMC, antigen presenting cells (APC) showed higher uptake of both NP preparations than lymphocytes. Furthermore, moDC internalized CS-PLGA NP more efficiently than PLGA NP, presumably because of receptor-mediated endocytosis. Consequently, CS-PLGA NP were delivered mostly to endosomal compartments, whereas PLGA NP primarily ended up in lysosomes. Thus, CS-PLGA NP confer enhanced delivery to endosomal compartments of APC, offering new therapeutic options to either induce or modulate APC function and to inhibit pathogens that preferentially infect APC. (C) 2019 The Author(s). Published by Elsevier Inc.
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