Article
Biochemical Research Methods
Stefan J. Tekel, Nicholas Brookhouser, Kylie Standage-Beier, Xiao Wang, David A. Brafman
Summary: The introduction of transient reporters of editing enrichment (TREE) has enabled highly efficient single-base editing of human cells using a transient episomal fluorescent reporter, allowing for rapid generation of clonal editing efficiencies exceeding 80% in biallelic or multiplexed edited isogenic human pluripotent stem cell lines within approximately 3-4 weeks.
Review
Agriculture, Dairy & Animal Science
Julia Popova, Victoria Bets, Elena Kozhevnikova
Summary: Genome editing has practical applications in farm animals, improving production traits, economic value, and disease resistance. It also has potential in biomedical research and drug production, as well as xenograft donors. Recent advancements in site-specific nucleases and embryological delivery methods have revolutionized transgenesis, providing efficient and reliable tools for genome engineering in agriculture.
Review
Biochemistry & Molecular Biology
Yulin Mu, Chengxiao Zhang, Taihua Li, Feng-Jie Jin, Yun-Ju Sung, Hee-Mock Oh, Hyung-Gwan Lee, Long Jin
Summary: Lactobacillus, important in food production and probiotics, can benefit from CRISPR/Cas9-based genome editing to improve strain efficiency and achieve traceless genome modification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Xueli Zhang, Chaohui Zhang, Caijiao Liang, Bizhou Li, Fanmei Meng, Yuncan Ai
Summary: Bacteriophages, the most abundant organisms in the biosphere, have been sequenced extensively. However, the study of bacteriophage functional genomics has been hindered by a lack of effective research methods. This study designed a phage genome editing platform based on the CRISPR-Cas9 system, and successfully achieved gene editing in Vibrio natriegens phage TT4P2. This platform has the potential to advance research on phage gene diversity and accelerate the development of phage synthetic biology and nanotechnology.
MICROBIOLOGY SPECTRUM
(2022)
Review
Pharmacology & Pharmacy
Siwei Chen, Deng Chen, Bin Liu, Hidde J. Haisma
Summary: CRISPR/Cas9-mediated genome engineering has extensive applications in basic biology, biotechnology, and medicine. However, the low gene modification efficiency and uncontrollable prolonged Cas9 activity hinder its further use. Researchers have explored small molecules with clinical potential to precisely modulate CRISPR/Cas9 genome editing activity.
DRUG DISCOVERY TODAY
(2022)
Review
Biochemistry & Molecular Biology
Min Ho Lee, Jae Il Shin, Jae Won Yang, Keum Hwa Lee, Do Hyeon Cha, Jun Beom Hong, Yeoeun Park, Eugene Choi, Kalthoum Tizaoui, Ai Koyanagi, Louis Jacob, Seoyeon Park, Ji Hong Kim, Lee Smith
Summary: This review highlights the current and potential use of CRISPR-Cas9 in the management of autoimmune diseases. Several studies have shown that CRISPR-Cas9 can be utilized for immunomodulation, reducing cholesterol, and treating rare diseases. However, there is limited research on the treatment of autoimmune diseases using CRISPR-Cas9.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Kristina J. Tatiossian, Robert D. E. Clark, Chun Huang, Matthew E. Thornton, Brendan H. Grubbs, Paula M. Cannon
Summary: This study found that CRISPR-Cas9 indel signatures can be used to identify gRNAs that maximize HDR outcomes, with the frequency of deletions resulting from MMEJ repair of at least 3 base pairs better predicts HDR frequency. Existing tools can be repurposed to identify gRNAs that promote HDR.
Article
Plant Sciences
Syed Inzimam Ul Haq, Dianfeng Zheng, Naijie Feng, Xingyu Jiang, Feng Qiao, Jin-Sheng He, Quan-Sheng Qiu
Summary: This review summarizes the advances of CRISPR/Cas9 technology in plant genome editing and discusses its applications in forage breeding, as well as the potential and challenges.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Chemistry, Physical
Juhee Lee, Yoo Kyung Kang, Eonju Oh, Juhee Jeong, San Hae Im, Duk Ki Kim, Haeshin Lee, Sang-Gyu Kim, Keehoon Jung, Hyun Jung Chung
Summary: The study presents a cancer gene therapy strategy based on NanoRNP that efficiently blocks the PD-L1 immune checkpoint and induces an antitumor effect in vivo without the need for combination therapy. In vivo results demonstrate that NanoRNP can induce indels in target cells at high frequencies, significantly suppressing tumor growth.
CHEMISTRY OF MATERIALS
(2022)
Article
Multidisciplinary Sciences
Beate Rieblinger, Hicham Sid, Denise Duda, Tarik Bozoglu, Romina Klinger, Antonina Schlickenrieder, Kamila Lengyel, Krzysztof Flisikowski, Tatiana Flisikowska, Nina Simm, Alessandro Grodziecki, Carolin Perleberg, Andrea Bahr, Lucie Carrier, Mayuko Kurome, Valeri Zakhartchenko, Barbara Kessler, Eckhard Wolf, Lutz Kettler, Harald Luksch, Ibrahim T. Hagag, Daniel Wise, Jim Kaufman, Benedikt B. Kaufer, Christian Kupatt, Angelika Schnieke, Benjamin Schusser
Summary: Research on genetically modified animals has mostly focused on mice, but also includes species like pigs that are more physiologically similar to humans, and cross-species comparisons with phylogenetically distant species like chickens. CRISPR-Cas9 is a versatile genetic editing method applicable across various species. The successful generation of transgenic chickens and pigs that express Cas9 in all organs has confirmed the functionality of Cas9 across different target genes, cell types, and in vivo applications.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Chao Zhang, Shanhe Liu, Xuan Li, Ruixuan Zhang, Jun Li
Summary: CRISPR/Cas-based genome editing technologies have revolutionized plant science by allowing precise manipulation of plant genomes. Virus-induced genome editing (VIGE) has emerged as a powerful method for delivering CRISPR/Cas reagents into plant cells, with high editing efficiency and simplified process. This article provides an overview of CRISPR/Cas-based genome editing and focuses on the VIGE systems, types of viruses used for CRISPR/Cas9 cassette delivery, recent applications, and challenges and potential of VIGE in plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Chin-Kai Chuang, Wei-Ming Lin
Summary: Cas9-sgRNA is a key tool for genome editing, with its simplicity, unique RNA-guided DNA recognition activity, and independent nuclease activities. Different variants of Cas9, in combination with sgRNA, can achieve various effects on target site breakage. Additionally, engineered enzyme activities can be introduced using Cas9 derivatives, and methods for transient expression and biosafety have been developed to address off-target effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Peng Yang, Athena Yue-Tung Lee, Jingjing Xue, Shih-Jie Chou, Calvin Lee, Patrick Tseng, Tiffany X. Zhang, Yazhen Zhu, Junseok Lee, Shih-Hwa Chiou, Hsian-Rong Tseng
Summary: The CRISPR/Cas9 genome editing system has revolutionized the field of therapeutic applications for genetic diseases and cancers. Efficient delivery of the system in vivo remains a significant challenge. Non-viral nano-vectors offer advantages over viral vectors and can be used to deliver CRISPR/Cas9 cargoes for targeted gene editing. The article highlights recent advances in nano-vector delivery for treating cancers and genetic diseases and proposes strategies for future development.
Article
Multidisciplinary Sciences
Joonbum Lee, Dong-Hwan Kim, Madeline C. Karolak, Sangsu Shin, Kichoon Lee
Summary: In this study, we successfully generated genome-edited chicken and duck lines without the conventional PGC-mediated procedures by injecting an adenovirus containing the CRISPR-Cas9 system into avian blastoderms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Xing Yin, Romain Harmancey, David D. McPherson, Hyunggun Kim, Shao-Ling Huang
Summary: The efficient delivery of CRISPR/Cas remains a challenge in gene editing, but liposomes provide a promising approach to enhance the delivery of CRISPR/Cas and guide RNA. Liposomes facilitate endosomal escape and offer spatial and temporal control of cargo release, making them an effective tool for precise and efficient genetic modifications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Mei Hu, Jiao Zhang, Li Kong, Yulin Yu, Qian Hu, Ting Yang, Yi Wang, Kun Tu, Qi Qao, Xianya Qin, Zhiping Zhang
Summary: Researchers have developed a novel hybrid nanovesicle that combines chemotherapy drugs with immunotherapy, targeting tumor tissue and triggering an effective immunogenic cell death response, thus activating the immune system and enhancing tumor immunogenicity.
Article
Pharmacology & Pharmacy
Jiali Li, Xue Jiang, Lihuan Shang, Zhen Li, Conglian Yang, Yan Luo, Daoyu Hu, Yaqi Shen, Zhiping Zhang
Summary: The study successfully synthesized L-epigallocatechin gallate complexed Mn2+ nanoparticles with good biocompatibility, stability, pH sensitivity, and anti-tumor properties, potentially serving as an MRI contrast agent.
Review
Pharmacology & Pharmacy
Qi Qiao, Xiong Liu, Ting Yang, Kexin Cui, Li Kong, Conglian Yang, Zhiping Zhang
Summary: Nanomedicine has shown unique advantages in ARDS therapy by interacting with inflammatory cells through targeted delivery, reversing abnormal changes, and demonstrating good therapeutic effects while reducing toxicity.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Nanoscience & Nanotechnology
Kun Tu, Yulin Yu, Yi Wang, Ting Yang, Qian Hu, Xianya Qin, Jingyao Tu, Conglian Yang, Li Kong, Zhiping Zhang
Summary: The use of CHI in TNBC helps to enhance tumor immunogenicity, and combined with PD-L1 blockade therapy can further strengthen the anti-tumor effect, effectively inhibiting tumor growth.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Biomedical
Yongzhi Cui, Yuanyuan Guo, Li Kong, Jingyu Shi, Ping Liu, Rui Li, Yongtao Geng, Weihang Gao, Zhiping Zhang, Dehao Fu
Summary: The complex pathogenesis of osteoporosis makes it difficult to treat with conventional therapies. Researchers have developed an exosome delivery system based on exosomes secreted by mesenchymal stem cells derived from induced pluripotent stem cells. By modifying a bone-targeting peptide, the system can specifically deliver siRNA to osteoblasts and enhance therapeutic effects.
BIOACTIVE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Xianya Qin, Conglian Yang, Hongbo Xu, Runzan Zhang, Dan Zhang, Jingyao Tu, Yuanyuan Guo, Boning Niu, Li Kong, Zhiping Zhang
Summary: The biosynthesis of nanomedicine using tumor cells as a basis has shown potential for enhancing radiotherapy sensitivity and promoting immune responses. Au@MC38's intracellular biomineralization and exocytosis process can be regulated by cellular metabolites and other factors, potentially leading to effective immune responses through radio-sensitization. Additionally, the strategy has demonstrated an effective inhibition of primary and metastatic tumors, as well as improving survival benefits when combined with immune checkpoint blockade.
Article
Biotechnology & Applied Microbiology
Jiali Li, Shichao Li, Yang Li, Guanjie Yuan, Yaqi Shen, Yang Peng, Li Kong, Conglian Yang, Zhiping Zhang, Zhen Li
Summary: A magnetic resonance nanoprobe with STING activation character was synthesized and combined with platinum-based chemotherapeutics to enhance immunochemotherapy. In vitro experiments demonstrated that this therapeutic strategy can promote immune cell activation and inhibit tumor growth in vivo.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Lihuan Shang, Xue Jiang, Ting Yang, Hongbo Xu, Qi Xie, Mei Hu, Conglian Yang, Li Kong, Zhiping Zhang
Summary: A pH-responsive nanogel (NG@M) coated with cancer cell membrane has been developed for the co-delivery of chemotherapeutic and immunotherapeutic agents, showing promising potential in the combinational treatment of breast cancer.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Chemistry, Multidisciplinary
Conglian Yang, Li Kong, Zhiping Zhang
Summary: This article discusses the design principles and strategies of bioreactor-based drug delivery systems and summarizes their advantages and applications in various fields. It emphasizes the applications of bioreactor-based drug delivery systems in the treatment and diagnosis of cancer, inflammatory bowel disease, Alzheimer's disease, and other diseases.
Article
Biotechnology & Applied Microbiology
Qi Xie, Shichao Li, Xingxing Feng, Jingyu Shi, Yang Li, Guanjie Yuan, Conglian Yang, Yaqi Shen, Li Kong, Zhiping Zhang
Summary: A nano-system was developed to integrate imaging and combinational therapy for triple-negative breast cancer (TNBC). The system encapsulates drugs and photothermal transduction agents, and serves as an MRI contrast agent, achieving integrated diagnosis and treatment.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Qi Qiao, Xiong Liu, Kexin Cui, Xiaonan Li, Tianyi Tian, Yulin Yu, Boning Niu, Li Kong, Conglian Yang, Zhiping Zhang
Summary: Acute respiratory distress syndrome (ARDS) is a life-threatening condition for ICU patients, and there is currently no viable medication for its treatment. Researchers have developed a hybrid nanosystem called liposomes-nanovesicles (LNVs), which combines M2 macrophage-derived nanovesicles and lung-targeting liposomes. The LNVs demonstrate high accumulation in the lungs and can effectively deliver immunoregulatory functions to lung tissues, suppressing inflammation and improving therapeutic outcomes in ARDS mice. This versatile drug delivery platform shows great potential for ARDS therapy.
Article
Chemistry, Physical
Yang Li, Dan Zhang, Tianzi Shi, Yulin Yu, Yinmei Tian, Qi Xie, Jingyu Shi, Li Kong, Conglian Yang, Zhiping Zhang
Summary: Nanotechnology has been applied in the development of traditional Chinese medicine (TCM), where nanostructures are found intrinsically in clinically applied TCM mixtures. The role and potential value of these nanostructures in TCMMs remain unknown. However, a study on a specific TCMM called Qingxuechushi mixture demonstrated that nanoparticles can be the most efficient part, leading to the development of a novel nanoformulation with definite components and good therapeutic effect. This work integrates the advantages of TCMMs and nanotechnology, offering a new approach for TCM development.
Review
Materials Science, Biomaterials
Yixuan Zhou, Xingxing Feng, Hongbo Xu, Jing Guo, Conglian Yang, Li Kong, Zhiping Zhang
Summary: Inflammatory bowel disease (IBD) is a recurrent intestinal disease, and natural product molecules have become an important source of anti-inflammatory drugs for treating IBD. However, poor solubility of natural products restricts their application. Micro/nano drug delivery systems can improve solubility and achieve targeted delivery and responsive release of anti-inflammatory drugs in the colon. This review highlights the potential of these strategies as treatments for IBD.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Multidisciplinary
Xiong Liu, Qi Qiao, Xiaonan Li, Xiangjun Ou, Kexin Cui, Boning Niu, Conglian Yang, Li Kong, Zhiping Zhang
Summary: Acute respiratory distress syndrome (ARDS) is a severe respiratory disease with high mortality, but effective therapeutic drugs for clinical practice are lacking. This study constructed an apoptotic neutrophil-mediated drug delivery system for ARDS treatment. The system exhibited significant effects on reducing neutrophil infiltration, inhibiting cytokine storm, and promoting anti-inflammatory macrophage polarization.
Article
Materials Science, Biomaterials
Dan Zhang, Pengran Liu, Xianya Qin, Lu Cheng, Fuqian Wang, Xin Xiong, Chuanqi Huang, Zhiping Zhang
Summary: Drug delivery systems (DDS) are crucial in tumor vaccine construction and their therapeutic effect promotion. By utilizing the versatile binding sites and bioreduction ability of human serum albumin (HSA), self-generated gold nanoparticles (AuNPs) on HSA (HSA@AuNPs) can serve as a DDS that converts light into heat. These nanoparticles deliver peptides, enhance immune response, and achieve combined photothermal therapy and immunotherapy.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
