Review
Pharmacology & Pharmacy
Vishal Jhanji, Isabelle Billig, Gary Hin-Fai Yam
Summary: Corneal opacification is the fourth most common cause of blindness globally. The standard treatment for serious corneal scarring is corneal transplantation, but there are limitations such as long-term graft survival and the lifelong use of immunosuppressants. Research is focused on finding new technologies to modulate wound healing and prevent corneal opacification.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Pascale Desjardins, Rebecca Berthiaume, Camille Couture, Gaetan Le-Bel, Vincent Roy, Francois Gros-Louis, Veronique J. Moulin, Stephanie Proulx, Sylvain Chemtob, Lucie Germain, Sylvain L. Guerin
Summary: Corneal wound healing involves communication between different cell types, which is partially mediated by exosomes. This study isolated exosomes released by different corneal cell types and found that they can accelerate wound healing. Additionally, exosomes alter the expression pattern of regulated genes. These findings are important for understanding the mechanisms of corneal wound repair and may lead to new therapeutic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Paulina Escandon, Angela Liu, Sarah E. Nicholas, Asher Khan, Kamran M. Riaz, Dimitrios Karamichos
Summary: This study explores the role of salivary exosomes in corneal wound healing for the first time. The results show that stimulation with salivary exosomes can slow down wound closure in healthy individuals and diabetic patients, and it affects the expression of certain proteins. However, it is still unclear whether salivary exosomes can be used in clinical settings to restore corneal defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Sandra Schumann, Eva Dietrich, Charli Kruse, Salvatore Grisanti, Mahdy Ranjbar
Summary: The study established an ex vivo organ culture model for evaluating and modulating epithelial wound healing on porcine corneas. Results showed the model can mimic the in vivo situation and provide a valuable research platform to study the effects of different culture media, wound sizes, and Mitomycin C on wound healing.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Engineering, Biomedical
Suyue Han, Duy T. Nguyen, Yahya Modarres-Sadeghi, Juan Miguel Jimenez
Summary: A POD order reduction method was implemented to reduce the high dimensional dynamical system associated with wound healing cell migration to a low-dimensional approximation. The POD analysis generated representative modes of the prevalent cell trajectories. The shapes of the modes depended on the location of the cells and the characteristics of the fluid flow.
ANNALS OF BIOMEDICAL ENGINEERING
(2022)
Article
Multidisciplinary Sciences
Hongmei Xu, Yucheng Huo, Quan Zhou, Lianghao Abraham Wang, Pingqiang Cai, Bryant Doss, Changjin Huang, K. Jimmy Hsia
Summary: Wound healing through reepithelialization of gaps is regulated by the geometry of the gap, which may occur through different pathways. In addition to purse-string contraction, cell protrusion and lamellipodium extension are critical mechanisms for closure of wavy gaps. The direction of cell migration, gap bridging, and actin cable constriction depend on the perpendicular alignment, size, and negative curvature of the gap. The discoveries deepen our understanding of cell responses to curvature and provide insights for tissue repair and wound management strategies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Joung-Hee Kim, Eun-Yeong Kim, Kyu Jin Chung, Jung-Hee Lee, Hee-Jung Choi, Tae-Wook Chung, Keuk-Jun Kim
Summary: Mealworm and mealworm oil have been reported to have effects on antioxidant, anti-coagulation, anti-adipogenic, and anti-inflammatory activities. This study demonstrates that mealworm oil may promote wound healing by inducing fibroblast migration and expression of wound healing factors, as well as promoting endothelial cell tube formation and migration through VEGF/VEGFR-2-mediated signaling pathways. These findings suggest that mealworm oil could be a potential therapeutic agent for skin wound treatment.
Article
Engineering, Biomedical
Qiang Zhou, Victor H. Guaiquil, Matthea Wong, Alejandro Escobar, Evguenia Ivakhnitskaia, Ghasem Yazdanpanah, Hongwu Jing, Michael Sun, Joy Sarkar, Yuncin Luo, Mark Rosenblatt
Summary: The acellular porcine corneal stroma (APCS) hydrogel demonstrated optimal light transmittance and gelation properties, retaining critical corneal ECM components. It showed higher porosity, faster nutrition diffusion, improved proliferation of corneal cells, and enhanced viability and infiltration. The APCS-gel also improved corneal epithelial stemness and wound healing in vitro and in vivo, making it a promising option for clinical applications.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Vincent Yeung, Tancy C. Zhang, Ling Yuan, Mohit Parekh, John A. Cortinas, Eleni Delavogia, Audrey E. K. Hutcheon, Xiaoqing Guo, Joseph B. Ciolino
Summary: This study investigates the bidirectional communication between corneal epithelial cells and corneal stromal cells through extracellular vesicles (EVs). The researchers found that EVs released by corneal myofibroblasts can enhance corneal epithelial cell migration and proliferation. These findings may offer a novel therapeutic approach for corneal wound healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Jason A. Wagoner, Ken A. Dill
Summary: Myosin II is a biomolecular machine responsible for muscle contraction. Multiple motors act cooperatively on a single actin filament, and filament backsliding is crucial for the speed and efficiency of muscle contraction. Evolutionary adaptations have reduced backsliding, optimizing the collective action of myosin II motors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Chiara Porro, Antonio Pennella, Maria Antonietta Panaro, Teresa Trotta
Summary: Myosins are a superfamily of actin-based motor proteins that play important roles in various cellular functions, with non-muscle myosin II (NM II) being expressed in neurons and non-neuronal brain cells. In the nervous system, NM II modulates functions such as vesicle transport, phagocytosis, cell migration, and inflammation, and inhibition of NM II could be a potential therapeutic target for CNS diseases. Microglia cells, as the principal regulators of immune processes in the CNS, react to stimuli and produce mediators that contribute to neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Indranil Ghosh, Raman K. Singh, Manjari Mishra, Shobhna Kapoor, Siddhartha S. Jana
Summary: Cells can transition between blebbing and lamellipodia modes of migration by regulating the activity of ROCK and MLCK, which play a key role in determining cell migration behaviors.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Walker D. Short, Emily Steen, Aditya Kaul, Xinyi Wang, Oluyinka O. Olutoye, Hima V. Vangapandu, Natalie Templeman, Alexander J. Blum, Chad M. Moles, Daria A. Narmoneva, Timothy M. Crombleholme, Manish J. Butte, Paul L. Bollyky, Sundeep G. Keswani, Swathi Balaji
Summary: This study found that overexpression of IL-10 in dermal wounds recruits EPCs, increases vascular structures, and accelerates re-epithelialization.
Article
Cell Biology
Shivani Patel, Donna McKeon, Kimheak Sao, Changsong Yang, Nicole M. Naranjo, Tatyana M. Svitkina, Ryan J. Petrie
Summary: The study reveals that RhoA activity inhibits lamellipodia formation in high-pressure cells by increasing intracellular pressure and disrupting actomyosin contractility and water influx. Increasing osmotic pressure triggers a second RhoA pathway through nonmuscle myosin II to disrupt lamellipodia and elevate pressure.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Nanoscience & Nanotechnology
Min Jeong Shin, San Hae Im, Baekman Kim, Jieun Choi, Stephani Edwina Lucia, Wantae Kim, Jesse G. Park, Pilhan Kim, Hyun Jung Chung, Dong Ki Yoon
Summary: Using improper wound care materials may hinder wound healing and cause scar formation and infection. This study introduces a simple method to create a cell alignment scaffold that enhances cell growth and migration, facilitating the reproduction of cellular arrangements and tissue restoration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Ophthalmology
Tzu-Hsun Tsai, Yao-Lin Liu, I-Hsin Ma, Chien-Chia Su, Chao-Wen Lin, Luke Long-Kuang Lin, Chuhsing Kate Hsiao, I-Jong Wang
Summary: The prevalence of myopia among schoolchildren in Taiwan has increased rapidly from 1983 to 2017, with older age and time spent on near-work activities identified as major risk factors. The use of electronic devices has been associated with an increased risk of developing myopia.
Review
Medicine, Research & Experimental
Wen-Yi Wang, Camille Chen, Justine Chang, Lillian Chien, Yung-Feng Shih, Luke L. K. Lin, Chi Pui Pang, I-Jong Wang
Summary: This review provides insights into the mechanism and potential targets for clinical intervention in myopia pathogenesis, emphasizing the impact of environmental factors and the need for further research on the efficacy of pharmacotherapeutics and combination therapy.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Ophthalmology
Meng-Yin Lin, Yun-Dun Shen, Hsin-Yuan Tan, I-Jong Wang, I-C Lin
Summary: Femtosecond laser arcuate keratotomy (FS-AK) and toric intraocular lens (IOL) implantation are effective for correcting corneal astigmatism. Both procedures are safe and achieve good outcomes, with standard phacoemulsification with toric IOL implantation showing better visual acuity results at the 6-month follow-up compared to FLACS with FS-AK.
INTERNATIONAL OPHTHALMOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chia-Hsiung Liu, Zheng-Hua Huang, Shu-Chien Huang, Tzuu-Shuh Jou
Summary: It was found that the release of Prdx1 after cardiac surgery could compromise immune competence, leading to increased susceptibility to infections. By preventing the binding of Prdx1 to macrophages, the induction of IL-10 and endotoxin tolerance could be reduced.
Article
Ophthalmology
Chi-Juei Jeng, Yi-Ting Hsieh, Cheng-Li Lin, I-Jong Wang
Summary: Antiplatelet/anticoagulant therapy has a protective effect against the development of non-proliferative diabetic retinopathy in patients with type 2 diabetes mellitus. Single use of aspirin or dipyridamole is beneficial for the prevention of non-proliferative diabetic retinopathy.
Article
Engineering, Chemical
Zhi-Xuan Dai, Po-Jen Shih, Jia-Yush Yen, I-Jong Wang
Summary: This study investigated the effects of stretching and shear stress on cultured human keratocytes. The results showed that both stretching and shear stress inhibited cell proliferation and caused cell death. Continuous stretching had higher cell survival rates compared to cyclic stretching, indicating that cyclic stretching has a greater inhibitory effect on cell growth and proliferation.
Article
Ophthalmology
Hsin-Yu Liu, Hsiao Sang Chu, Wei-Li Chen, I-Jong Wang, Fung-Rong Hu
Summary: This study evaluated the utility rate, indication, outcome, and cost of refrigeration and glycerol cryopreservation for storing anterior corneal buttons during endothelial keratoplasty. The results showed that cryopreservation can safely extend the utility of anterior corneal buttons, reducing wastage and being cost-effective.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2023)
Letter
Ophthalmology
Mei-Chi Tsui, Bor-Luen Chiang, I-Jong Wang
CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wei-Ting Ho, Jung-Shen Chang, Tsan-Chi Chen, Jia-Kang Wang, Shu-Wen Chang, Muh-Hwa Yang, Tzuu-Shuh Jou, I-Jong Wang
Summary: ROCK inhibition promotes metabolic reprogramming towards OXPHOS and enhances migration of CECs.
Article
Medicine, Research & Experimental
Hsi-Yun Tai, Jun-Ji Lin, Yi-Hung Huang, Po-Jen Shih, I-Jong Wang, Jia-Yush Yen
Summary: This study investigated the correlation between corneal biomechanical properties and topographic parameters using machine learning networks for automatic severity diagnosis. The results showed strong correlations between symmetric modes and keratoconus severity, as well as asymmetric modes and the weak centroid location. Further studies are needed to gather more patient data for validation.
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2022)
Review
Ophthalmology
Mei-Chi Tsui, Hsin-Yu Liu, Hsiao-Sang Chu, Wei-Li Chen, Fung-Rong Hu, Winston W. -Y. Kao, I-Jong Wang
Summary: Lumican, a keratan sulfate proteoglycan, plays versatile roles in the pathogenesis of eye diseases. It is involved in the maintenance of tissue homogeneity and shows upregulation in pathological conditions such as fibrosis, scar tissue formation, inflammatory responses, and immune anomaly. This review focuses on the role of lumican in the pathogenesis of inherited congenital and acquired eye diseases such as cornea dystrophy, cataract, glaucoma, and chorioretinal diseases.
Article
Biochemistry & Molecular Biology
Jhan-Jhang Syu, Chieh-Hsiang Chang, Pei-Yu Chang, Chia-Hsiung Liu, Chia-Jung Yu, Tzuu-Shuh Jou
Summary: The study shows that galectin 8 promotes the rapid elongation of primary cilia by binding to lipid raft components at the base of cilia and interfering with the interaction between caveolin 1 and TMEM231. The functional barrier of the transition zone is disrupted, resulting in the redistribution of cilia proteins and the elongation of primary cilia.
Article
Ophthalmology
Chien-Chia Su, Chia-Mao Gao, Fu Ti Peng, Tzuu-Shuh Jou, I-Jong Wang
Summary: This study investigated the pathogenesis of CMV-associated anterior segment infection and evaluated the effects of ganciclovir and glucocorticoid treatment. An inoculation model was used to reproduce the infection in rats, and various methods were used to analyze the immune response. The results showed that early ocular immune response reduced virus DNA and alleviated lymphadenopathy, and ganciclovir therapy enhanced cytokine expression. However, glucocorticoid treatment hindered viral clearance.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
