Review
Biochemical Research Methods
Jiashu Liu, Cui-Xiang Lin, Xiaoqi Zhang, Zongxuan Li, Wenkui Huang, Jin Liu, Yuanfang Guan, Hong-Dong Li
Summary: Alternative splicing (AS), a key pathway for transcriptional regulation, has been shown to be associated with complex diseases. Computational approaches for detecting disease-associated AS events have been developed. This review discusses the metrics used for characterizing AS events quantitatively. It also reviews and discusses three types of methods for detecting disease-associated splicing events: differential splicing analysis, aberrant splicing detection, and splicing-related network analysis. Additionally, methods for detecting genetic variants that potentially regulate splicing are described. Experimental comparisons are conducted to illustrate the performance of each method. The limitations of these methods are discussed, as well as potential ways to address them. This review aims to provide a systematic understanding of computational approaches for analyzing disease-associated splicing.
BRIEFINGS IN BIOINFORMATICS
(2023)
Review
Biochemistry & Molecular Biology
Yunqi Chao, Yonghui Jiang, Mianling Zhong, Kaiyan Wei, Chenxi Hu, Yifang Qin, Yiming Zuo, Lili Yang, Zheng Shen, Chaochun Zou
Summary: Alternative splicing plays a crucial role in regulating gene expression patterns post-transcriptionally, particularly in adipogenesis. Aberrant expression and alterations in splicing regulators are linked to mutations in obesity-related genes, indicating their potential role in diet-induced obesity and metabolic dysregulation. Research on the splicing machinery's interaction with obesity and metabolic disorders provides insights for potential therapeutic strategies in clinical settings.
CELL AND BIOSCIENCE
(2021)
Article
Oncology
Jia Liu, Dekang Lv, Xiaobin Wang, Ruicong Wang, Xiaodong Li
Summary: This study systematically analyzed the survival-related AS signal in ovarian serous cystadenocarcinoma and established a new comprehensive prognostic model based on seven AS events. The models were highly efficient in distinguishing patient survival and showed higher prediction ability than mRNA signature, which could be used as potential predictor and therapeutic target for patients with OV.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemical Research Methods
Guangyu Yang, Leslie Cope, Zitong He, Liliana Florea
Summary: This study introduces a toolkit called Jutils for visualizing differential splicing events at the intron level. Jutils is method-agnostic, converting outputs from different tools into a unified representation to create heatmaps, sashimi plots, and Venn diagrams. The tool is lightweight and relies solely on a unified data file for visualizations.
Review
Biochemistry & Molecular Biology
Woo Ryung Kim, Eun Gyung Park, Yun Ju Lee, Woo Hyeon Bae, Du Hyeong Lee, Heui-Soo Kim
Summary: Alternative splicing of mRNA plays a crucial role in genetic diversity and cancer development. Transposable elements can induce alternative splicing in cancer cells through different mechanisms, and also control the overall gene expression process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Zeng-Hong Wu, Yun Tang, Yue Zhou
Summary: Alternative splicing (AS) is a critical post-transcriptional regulatory mechanism that increases protein diversity by expanding gene expression patterns. In this study, an all-around AS profile of thyroid cancer cells was drawn based on RNA-seq data, revealing that ASEs are common in TC. Additionally, 1819 AS signatures were significantly associated with the overall survival of TC patients, with seven types of ASEs linked to poor prognosis of TC.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Huan Qi, Xiaokun Guo, Tianpeng Wang, Ziding Zhang
Summary: In this study, a bioinformatics tool named ASTool was developed for detecting AS events from plant RNA-Seq data. ASTool demonstrated good performance and competitive computational speed, providing consistent detection results in simulated and real plant RNA-Seq data.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Xu Zhang, Xiaofeng Yin, Lichao Zhang, Zhiqiang Ye, Guangmin Liang
Summary: This study aimed to explore the importance of alternative splicing (AS) events in uterine carcinosarcoma (UCS), construct an AS-based prognosis model, and identify key splicing factors (SFs). Through data collection and analysis, seven AS events related to survival were detected and a risk score was obtained. The prognosis model showed high prediction accuracy and the identified SFs could act as potential drug targets and prognosis biomarkers.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Oncology
Boxue He, Cong Wei, Qidong Cai, Pengfei Zhang, Shuai Shi, Xiong Peng, Zhenyu Zhao, Wei Yin, Guangxu Tu, Weilin Peng, Yongguang Tao, Xiang Wang
Summary: This study evaluated AS-based biomarkers for lung squamous cell carcinoma (LUSC) patients using machine learning algorithms. Through data analysis, AS events related to LUSC were identified, and a LNM classifier and prognostic model were built. Validation experiments confirmed the reliability of these events.
CANCER CELL INTERNATIONAL
(2022)
Article
Immunology
Xueyi Song, Xuehao Li, Yuexin Ge, Jia Song, Qian Wei, Miao He, Minjie wei, Yining Zhang, Tianbao Chen, Lin Zhao
Summary: Alternative splicing controls gene expression and produces distinct protein heterodimers. Aberrant alternative splicing affects cell development and plays a role in cancer invasion and metastasis. Recent research shows that alternative splicing can alter the tumor microenvironment and regulate processes such as remodeling, immunity, and inflammation. However, there is a lack of comprehensive literature review on the complex relationship between alternative splicing and the tumor microenvironment. This review aims to provide new perspective on the therapeutic and prognostic markers of cancer by collecting the latest data on this topic.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Oncology
Ping Ye, Yan Yang, Liqiang Zhang, Guixi Zheng
Summary: This study identified 2,212 AS events significantly associated with overall survival in esophageal carcinoma patients from TCGA database. Prognostic signatures based on these AS events were established through multivariate analysis, with risk score proving to be an independent survival factor. The interaction network between AS events and splicing factors revealed hub genes and AS events warranting further investigation, providing novel insights into tumorigenesis and progression mechanisms of esophageal carcinoma.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Jun Yao, Yu-Chen Tang, Bin Yi, Jian Yang, Yun Chai, Ni Yin, Zi-Xiang Zhang, Yi-Jun Wei, De-Chun Li, Jian Zhou
Summary: Alternative splicing plays a crucial role in the development and progression of cancer, with potential implications for patient prognosis. By analyzing RNA-Seq data from PAAD patients, differentially expressed AS events were identified, revealing associations with patient clinical characteristics and survival patterns. Additionally, splicing factors such as ESRP1 and RBM5 were found to be important in PAAD-related AS events, providing valuable insights for future therapeutic strategies.
Review
Oncology
Mosebo Armstrong Manabile, Rodney Hull, Richard Khanyile, Thulo Molefi, Botle Precious Damane, Nigel Patrick Mongan, David Owen Bates, Zodwa Dlamini
Summary: Colorectal cancer is a leading cause of cancer-related deaths globally, with increasing incidence in developing countries. Dysregulated alternative splicing processes in colorectal cancer can promote its development and progression by producing proteins that activate cancer-promoting genes or deactivate cancer-inhibiting genes. It is important to identify these dysregulated alternative splicing genes in order to develop targeted treatments and diagnostics to stop cancer development and progression.
Article
Cell Biology
Jichang Liu, Yadong Wang, Xiaogang Zhao, Kai Wang, Chao Wang, Jiajun Du
Summary: This study reveals the significant association between splicing factors and tumor microenvironment remodeling. Evaluating different splicing factor patterns enhances our understanding of the tumor microenvironment and improves effective immunotherapy strategies.
Article
Cell Biology
Yunjie Wang, Zexu Li, Guanheng Yang, Linlin Cai, Fan Yang, Yaqiong Zhang, Yitao Zeng, Qingwen Ma, Fanyi Zeng
Summary: Down's syndrome is a disorder with multiple congenital disabilities, which can be associated with severe cognitive impairment, intellectual disability, and other comorbid conditions. Studies have found a significant number of up-regulated and down-regulated genes in individuals with DS, which are enriched in biological processes such as cell adhesion, cardiac muscle contraction, and immune response. This research provides insights into the potential role of alternative splicing in DS.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Xia Yi, Ping Wu, Jianyun Liu, Ying Gong, Xiaoyuan Xu, Weidong Li
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Article
Cell Biology
Xia Yi, Jianyun Liu, Ping Wu, Ying Gong, Xiaoyuan Xu, Weidong Li
JOURNAL OF CELLULAR PHYSIOLOGY
(2020)
Article
Cell Biology
Xia Yi, Jianyun Liu, Ping Wu, Ying Gong, Xiaoyuan Xu, Weidong Li
JOURNAL OF CELLULAR PHYSIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Xia Yia, Qiuqiang Gao, Jie Bao
JOURNAL OF BIOTECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Xia Yi, Jun Mei, Ling Lin, Wei Wang
Summary: The study identified a dioxygenase encoding gene ZMO1721 that was differentially expressed under the stress of 4-hydroxybenzaldehyde in Z. mobilis ZM4, which was confirmed to have NADH-dependent reduction activity in phenolic aldehyde vanillin conversion. Overexpression of ZMO1721 gene improved the ethanol productivity and growth rate of Z. mobilis ZM4. These findings could serve as synthetic biology tools for modifying ethanologenic strains.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xia Yi, Ling Lin, Jun Mei, Wei Wang
Summary: Transporter proteins play a crucial role in improving the tolerance of fermentation strains to lignocellulose-derived furans and phenolic inhibitors. A study identified six transporter proteins as potential candidates responsible for the tolerance of phenolic aldehydes in Zymomonas mobilis, highlighting their significant correlation with oxidoreductases and transcriptional regulators. This research provides important transporter genes as synthetic biology tools for enhancing the robustness of biorefinery strains.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Xia Yi, Jianfang Wu, He Jiang, Yan Zhao, Jun Mei
Summary: The expression of certain kinase genes under phenolic aldehydes stress affects the conversion of aldehydes and the fermentability of ethanol in Zymomonas mobilis.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Bin Zhang, Mesfin Geberekidan, Zhao Yan, Xia Yi, Jie Bao
Summary: A highly thermotolerant strain of Saccharomyces cerevisiae, S. cerevisiae Z100, was obtained through laboratory adaptive evolution. The cell survival ratio of S. cerevisiae Z100 at 50 degrees C increased by 1.2 times compared to the parental strain, resulting in higher ethanol production during cellulosic ethanol fermentation. Transcriptome analysis suggested that enhanced trehalose and glycogen synthesis, as well as carbon metabolism, contributed to the improved thermotolerance and ethanol fermentability of S. cerevisiae Z100.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Xia Yi, Dong Yang, Xiaoyan Xu, Youjun Wang, Yan Guo, Meng Zhang, Yilong Wang, Yucai He, Jie Zhu
Summary: By pretreating Zymomonas mobilis ZM4 with cold plasma, this study improved its tolerance to aldehyde inhibitors and its fermentability for cellulosic bioethanol production.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Xia Yi, Ping Wu, Jianyun Liu, Shan He, Ying Gong, Jianjun Xiong, Xiaoyuan Xu, Weidong Li
Summary: This study identified important regulatory genes in adipo-osteoblastogenesis by constructing the transcriptional landscapes of kinases. PTK2B and PRKG2, as key kinases involved in protein phosphorylation, may serve as potential molecular switches for controlling cell fate determination in hMSCs.