Article
Environmental Sciences
Lijia Zhao, Jing Zhang, Luda Yang, Haisen Zhang, Yu Zhang, Dengke Gao, Haizhen Jiang, Yating Li, Hao Dong, Tiantian Ma, Xiaoyu Wang, Meina Wu, Aihua Wang, Yaping Jin, Yalin Yuan, Huatao Chen
Summary: Glyphosate, a broad-spectrum herbicide, impairs testosterone synthesis in mammals, potentially through disrupting the circadian clock system via NR1D1. Exposure to glyphosate reduces testosterone production, affecting fertility in both male and female mice.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Cuimei Li, Linlin Zhang, Tiantian Ma, Lei Gao, Luda Yang, Meina Wu, Zhaoxia Pang, Xiaoyu Wang, Qiyang Yao, Yaoyao Xiao, Lijia Zhao, Wei Liu, Hongcong Zhao, Caixia Wang, Aihua Wang, Yaping Jin, Huatao Chen
Summary: This study demonstrates that BPA disrupts testosterone production by inhibiting NR1D1 signaling in Leydig cells, leading to a decrease in testosterone levels in mice. Additionally, circadian clock genes are also involved in this process.
Article
Cell Biology
Marija Lj Medar, Silvana A. Andric, Tatjana S. Kostic
Summary: This study investigates the stress response of testosterone-producing rat Leydig cells depending on the time of stressful events. The findings show that the stress response of Leydig cells varies depending on the diurnal time, highlighting the importance of the circadian system in regulating androgen homeostasis and male fertility.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Medicine, Research & Experimental
Lingling Zhang, Lijun Ding, Yifan Li, Fangxi Zhang, Yanhong Xu, Hongjie Pan, Xiaofeng Wan, Guijun Yan, Fei Yu, Runsheng Li
Summary: This study demonstrates that EHD3 plays a role in promoting testosterone synthesis, with NR5A1 positively modulating its promoter activity to regulate endocytosis. Conditional knockout of NR5A1 in Leydig cells results in decreased levels of EHD3 and clathrin, impacting serum testosterone concentration. These findings provide insights into the molecular mechanisms of testosterone synthesis for better understanding of male reproductive health.
Article
Environmental Sciences
Lu Lu, Jing-Bo Liu, Jin-Qiu Wang, Cai-Yu Lian, Zhen-Yong Wang, Lin Wang
Summary: Glyphosate (GLY) induces mitochondrial dysfunction and mitochondrial reactive oxygen species (mtROS) overproduction, leading to the activation of parkin-dependent mitophagy and ultimately inhibiting testosterone synthesis.
ENVIRONMENTAL POLLUTION
(2022)
Article
Veterinary Sciences
Yan Lin, De Wu, Lianqiang Che, Zhengfeng Fang, Shengyu Xu, Bin Feng, Yong Zhuo, Jian Li, Caimei Wu, Junjie Zhang, Lujie Li
Summary: This study investigated the effects of fibre supplementation on testis development in growing boars and found that it increased semen production without affecting growth performance or testicular volume. Fibre supplementation resulted in lower serum cholesterol and LDL levels, but higher testicular cholesterol, triglyceride, and LDL levels. It also increased Leydig cell density and up-regulated the expression of genes related to hormone synthesis.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Environmental Sciences
Lijia Zhao, Yaoyao Xiao, Cuimei Li, Jing Zhang, Yaojia Zhang, Meina Wu, Tiantian Ma, Luda Yang, Xiaoyu Wang, Haizhen Jiang, Qian Li, Hongcong Zhao, Yiqun Wang, Aihua Wang, Yaping Jin, Huatao Chen
Summary: The study suggests that Zearalenone (ZEA) may impair testosterone synthesis by attenuating the circadian clock in Leydig cells (LCs), leading to reduced protein levels and testosterone secretion, ultimately resulting in reproductive dysfunction in male mammals.
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
(2021)
Article
Cell Biology
Marija Lj Medar, Silvana A. Andric, Tatjana S. Kostic
Summary: The study reveals the time-dependent effects of stress on gene expression in Leydig cells, connecting them to glucocorticoid signaling. Stress leads to changes in corticosterone and testosterone levels, affecting steroidogenesis-related genes and clock genes in Leydig cells. Stressful stimuli can entrain the clock in Leydig cells through glucocorticoid-mediated communication.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Article
Obstetrics & Gynecology
Betcy Susan Johnson, Meera B. Krishna, Renjini A. Padmanabhan, Sathy M. Pillai, K. Jayakrishnan, Malini Laloraya
Summary: The study found that circadian genes show altered expression in peripheral blood mononuclear cells of polycystic ovary syndrome (PCOS) patients, potentially affecting steroid metabolism. The variations in gene expression are associated with different phenotypes of PCOS patients.
HUMAN REPRODUCTION
(2022)
Article
Reproductive Biology
Hong Chen, Kexing Chen, Fange Zhao, Yihan Guo, Yue Liang, Zhengrong Wang, Tengfei Liu, Shulin Chen
Summary: This study investigated the steroidogenic activity and autophagy levels of Leydig cells (LCs) in dairy goats at different stages of development. The results showed that the steroidogenic activity and ultrastructural characteristics of LCs changed with age, and autophagy activity was enhanced in the testes at pubertal and adult stages. In addition, the study found that autophagy affects testosterone production in LCs.
Article
Reproductive Biology
Minghui Yang, Shengyu Guan, Jingli Tao, Kuanfeng Zhu, Dongying Lv, Jing Wang, Guangdong Li, Yuefeng Gao, Hao Wu, Jinghao Liu, Lin Cao, Yao Fu, Pengyun Ji, Zhengxing Lian, Lu Zhang, Guoshi Liu
Summary: The study found that endogenous melatonin can regulate male reproductive function by inhibiting Leydig cell apoptosis, increasing testosterone production, and improving sperm quality. Melatonin exerts these effects by targeting the mitochondrial apoptotic pathway and upregulating testosterone synthesis-related genes in Leydig cells.
BIOLOGY OF REPRODUCTION
(2021)
Article
Genetics & Heredity
Liangfeng Huang, Kai Xiao, Junjun Zhang, Pengfei Zhang, Wengtan He, Yuyan Tang, Weihan Yang, Xingchen Huang, Runfeng Liu, Xianwei Liang, Xingting Liu, Qiang Fu, Yangqing Lu, Ming Zhang
Summary: This study analyzed the transcriptome profiles of immature Leydig cells (ILC) and adult Leydig cells (ALC) in buffalo, identifying key genes and pathways associated with the regulation of testosterone secretion function. The findings suggest that cAMP signaling plays a crucial role in maintaining testosterone function during postnatal development of LCs, with ALCs potentially increasing testosterone levels through enhanced cAMP production compared to ILCs. Protein-protein interaction analysis highlighted hub genes in cAMP signaling pathway, providing insights into molecular mechanisms of LCs regulating spermatogenesis.
Article
Endocrinology & Metabolism
Hongzhou Guo, Di Zhang, Yewen Zhou, Longjie Sun, Changping Li, Xuan Luo, Jiali Liu, Sheng Cui
Summary: Our study showed that CK1 alpha is expressed in both germ cells and somatic cells of mouse testes and plays a crucial role in regulating testosterone synthesis and male reproduction. We found that conditional disruption of CK1 alpha in Leydig cells significantly reduced testosterone levels, affected testis development, sperm motility, and sperm morphology, and caused subfertility. Furthermore, we discovered that luteinizing hormone can upregulate CK1 alpha through a specific signaling pathway and that CK1 alpha can interact with and phosphorylate EGFR, leading to the activation of ERK1/2 and the promotion of testosterone synthesis.
Article
Cell Biology
Yabing Chen, Jing Wang, Dihui Xu, Zou Xiang, Jie Ding, Xiaoyu Yang, Dongmei Li, Xiaodong Han
Summary: The study reveals a negative association between m(6)A modification and autophagy in Leydig cells during testosterone synthesis. The regulation of METTL14, ALKBH5, and m(6)A influences AMP-activated protein kinase activity and subsequent autophagy in Leydig cells. Human chorionic gonadotropin treatment modulates m(6)A modification by upregulating ALKBH5 and decreasing METTL14, affecting the synthesis of testosterone.
Article
Cell Biology
Xiaofan Xiong, Qiuhua Wu, Lingyu Zhang, Shanfeng Gao, Rufeng Li, Lin Han, Meiyang Fan, Miaomiao Wang, Liying Liu, Xiaofei Wang, Chunli Zhang, Yanlong Xin, Zongfang Li, Chen Huang, Juan Yang
Summary: Chronic stress was found to lead to weight loss, decreased genital index, and lower serum testosterone levels in adult male rats. Proteomic analysis revealed that ATP5A1 expression in Leydig cells is significantly reduced after chronic stress, affecting testosterone synthesis.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)