Article
Developmental Biology
Elvin Leonard, Sana Safatul Hasan, Arndt F. Siekmann
Summary: Blood vessels in the zebrafish caudal fin form through a stepwise process involving different morphogenetic processes, including artery generation from vein-derived endothelial cells, production of veins by veins, formation of distinct vascular plexuses, and angiogenesis. These processes are regulated by a combination of signaling pathways and local cues. The study provides insights into the diverse mechanisms underlying the assembly of mature vasculature.
Review
Biochemistry & Molecular Biology
Selvaraj Vimalraj
Summary: Angiogenesis is the formation of new blood vessels from existing ones. Abnormal angiogenesis, seen in tumor microenvironments, is characterized by distorted and leaky blood vessels with various shapes and high perfusion efficiency. Tumor angiogenesis plays a crucial role in cancer growth, invasion, and metastasis, and is tightly regulated by signaling networks. Targeting multiple signaling pathways involved in angiogenesis is important for effective antiangiogenic therapy. New therapeutic approaches, such as blocking specific proteins or genes, are being explored to dissect alternative angiogenesis mechanisms in tumor microenvironments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Multidisciplinary Sciences
Zhiyang Li, Heng Zhang, Yujie Sun, Zhuangzhuang Feng, Bijia Cui, Jingxia Han, Yinan Li, Huijuan Liu, Tao Sun
Summary: Angiogenesis plays a crucial role in embryologic development, growth, and tissue repair, but dysregulation of this process is a hallmark of various pathologies, including cancer. Existing methods for evaluating cell vascular formation are often limited to static analysis, leading to bias. Researchers have developed code scripts to study the dynamic angiogenesis process, allowing for the screening of drugs that can affect various parameters of vascular formation. Animal experiments have confirmed the efficacy of these drugs in inhibiting blood vessel formation, providing a new perspective for angiogenesis research and drug development.
Review
Biology
Jean Eberlein, Lukas Herdt, Julian Malchow, Annegret Rittershaus, Stefan Baumeister, Christian S. M. Helker
Summary: Establishing a functional cardiovascular system is crucial for vertebrate development, with defects leading to cardiovascular diseases. Zebrafish has been instrumental in identifying novel mechanisms of vascular growth, particularly in understanding the molecular pathways involved in blood vessel formation within different tissues and organs.
Article
Ophthalmology
Yueru Li, Basma Baccouche, Olamide Olayinka, Anara Serikbaeva, Andrius Kazlauskas
Summary: The study reveals that VEGF/anti-VEGF controls endothelial cell permeability by regulating multiple members of the Wnt pathway, and activating this pathway helps strengthen the endothelial cell barrier. FDA-approved agents such as LiCl may serve as adjuvants to anti-VEGF therapy for blinding conditions like diabetic retinopathy.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Manusmriti Agarwal, Ankush Sharma, R. Andrea Kagoo, Anamika Bhargava
Summary: As the global demand for new synthetic compounds continues to grow, the adverse health effects of these compounds have become a critical public health concern. Zebrafish, with their genomic similarity to humans, optical clarity, well-defined developmental stages, short generation time, and cost-effective maintenance, have emerged as a prominent model organism for toxicology studies. The use of zebrafish allows for faster in vivo toxicology evaluation compared to mammalian experimental systems.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Christina M. Termini, Amara Pang, Tiancheng Fang, Martina Roos, Vivian Y. Chang, Yurun Zhang, Nicollette J. Setiawan, Lia Signaevskaia, Michelle Li, Mindy M. Kim, Orel Tabibi, Paulina K. Lin, Joshua P. Sasine, Avradip Chatterjee, Ramachandran Murali, Heather A. Himburg, John P. Chute
Summary: Ionizing radiation and chemotherapy deplete hematopoietic stem cells and damage the vascular niche, leading to the secretion of SEMA3A from bone marrow endothelial cells. Inhibition of NRP1 promotes vascular and hematopoietic regeneration through increased expression of R spondin 2, suggesting a potential therapeutic target for improving hematopoietic recovery after myeloablation.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Amirfarbod Yazdanyar, Charles L. Cai, Jacob V. Aranda, Eric Shrier, Kay D. Beharry
Summary: This study found that Eylea is not as effective as Avastin in promoting angiogenesis, although the lower dose of Eylea may be more effective than the higher dose. Additionally, Eylea may promote normal vascular development by regulating the expression of factors such as VEGFR-1 and Notch/Jagged-1.
Article
Oncology
Yanfen Cui, Hui Liu, Zhaosong Wang, He Zhang, Jianfei Tian, Zhiyong Wang, Weijie Song, Hui Guo, Liming Liu, Ruinan Tian, Xiaoyan Zuo, Sixin Ren, Fei Zhang, Ruifang Niu
Summary: High fructose intake is associated with increased risk of multiple cancers. Fructose can enhance the biological function of vascular endothelial cells (VECs) and promote angiogenesis by activating the Akt and Src signaling pathways. It can also upregulate production of reactive oxygen species (ROS) in colorectal cancer cells, indirectly enhancing the function of VECs. These findings have been validated in clinical colorectal cancer tissues and mouse models.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Environmental Sciences
Saifang Fu, Rongbang Tan, Yufei Feng, Ping Yu, Yuqian Mo, Wei Xiao, Shouyu Wang, Jingjing Zhang
Summary: This study revealed that N-methyl-N-nitrosourea (MNU) induced aberrant angiogenesis through the modulation of the rspo1/Wnt/β-catenin/vegfaa axis. Furthermore, it was found that the pro-angiogenic effect of MNU on developing zebrafish embryos could be controlled through pharmacological manipulation.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Review
Biochemistry & Molecular Biology
Mengyuan Cao, Yunmeng Wang, Guige Lu, Haoran Qi, Peiyu Li, Xiaoshuo Dai, Jing Lu
Summary: Although progress has been made in CRC research, CRC still remains the second most frequent type of malignancy and the major cause of cancer-related death among gastrointestinal cancers. Understanding the key signaling pathways that regulate CRC angiogenesis is critical in inhibiting CRC.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Review
Cell Biology
Mia C. Borlongan, Hongbin Wang
Summary: Tumorigenic cancer stem cells (CSCs) are a subset of cells within tumors that have distinct genetic and phenotypic profiles and signaling pathways compared to other tumor cells. Targeting the unique self-renewal and differentiation properties of CSCs could revolutionize cancer therapy. Understanding the signaling mechanisms of CSCs will improve our knowledge of cancer pathology and treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Developmental Biology
Martin Lange, Nils Ohnesorge, Dennis Hoffmann, Susana F. Rocha, Rui Benedito, Arndt F. Siekmann
Summary: Studies in zebrafish show that vegfaa and vegfab have different effects on endothelial cell migration and proliferation. Mutations in vegfaa impact both migration and proliferation, while mutations in vegfab more strongly affect the proliferation of endothelial cells in specific blood vessels, potentially through the PI3K signaling pathway.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Shu Yan, Ji Hu, Jia Li, Pengchao Wang, Yilong Wang, Zhaohui Wang
Summary: PRMT4 is upregulated in ischemic muscles and promotes angiogenesis. It regulates VEGF expression by interacting with YB1. Knockdown of YB1 attenuates PRMT4-mediated angiogenic effects in vitro.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2021)
Article
Behavioral Sciences
Wenyu Zhang, Lin Han, Yan Wen, Lixian Su, Yibing Li, Xudong Luo
Summary: This study found that electroacupuncture (EA) can promote angiogenesis and provide brain protection through the activation of the VEGF/Notch signaling pathway after ischemic stroke.
BRAIN AND BEHAVIOR
(2023)
Article
Multidisciplinary Sciences
Karin D. Prummel, Helena L. Crowell, Susan Nieuwenhuize, Eline C. Brombacher, Stephan Daetwyler, Charlotte Soneson, Jelena Kresoja-Rakic, Manuel Ronner, Agnese Kocere, Alexander Ernst, Zahra Labbaf, David E. Clouthier, Anthony B. Firulli, Hector Sanchez-Iranzo, Sundar R. Naganathan, Rebecca O'Rourke, Erez Raz, Nadia Mercader, Alexa Burger, Emanuela Felley-Bosco, Jan Huisken, Mark D. Robinson, Christian Mosimann
Summary: This study uncovers the early origins of mesothelium progenitor cells in zebrafish, linking the function of the Hand2 gene to mesothelium formation and the re-activation of mesothelioma tumors.
NATURE COMMUNICATIONS
(2022)
Article
Developmental Biology
Elisabeth Kugler, Isabel Bravo, Xhuljana Durmishi, Stefania Marcotti, Sara Beqiri, Alicia Carrington, Brian Stramer, Pierre Mattar, Ryan B. MacDonald
Summary: Cell morphology is important for cell functions, especially for glial cells that rely on complex shape for neuron support. However, accurate and reproducible methods to quantify complex glial cell shape are lacking. To address this, the image analysis pipeline 'GliaMorph' was developed, enabling in-depth understanding of glial cell morphology in the developing and diseased retina.
Article
Hematology
Lindsay Canham, Sam Sendac, Mannekomba R. Diagbouga, Elena Wolodimeroff, Daniela Pirri, Blanca Tardajos Ayllon, Shuang Feng, Celine Souilhol, Timothy J. A. Chico, Paul C. Evans, Jovana Serbanovic-Canic
Summary: Hemodynamic wall shear stress (WSS) exerted on the endothelium by flowing blood determines the spatial distribution of atherosclerotic lesions. Disturbed flow (DF) with low WSS promotes atherosclerosis, while unidirectional flow with high WSS is protective. This study investigates the role of EVA1A in WSS-regulated endothelial cell dysfunction.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Cell Biology
Ryan O. Snodgrass, Karan Govindpani, Karen Plant, Elisabeth C. Kugler, Changmin Doh, Thomas Dawson, Luis E. McCormack, Helen M. Arthur, Timothy J. A. Chico
Summary: Hereditary haemorrhagic telangiectasia (HHT) is caused by mutations in the BMP co-receptor endoglin, leading to arteriovenous malformations (AVMs) and various complications. Zebrafish models were used to study the vascular phenotypes associated with endoglin mutations and the effect of inhibiting Vegf signalling pathways. Inhibiting mTOR or MEK pathways prevented abnormal vasculature phenotypes, suggesting a potential therapeutic strategy in HHT. These findings highlight the importance of Vegf signalling modulation in mitigating HHT-like phenotypes.
DISEASE MODELS & MECHANISMS
(2023)
Article
Health Care Sciences & Services
William G. Dixon, Sabine N. van der Veer, Syed Mustafa Ali, Lynn Laidlaw, Richard J. B. Dobson, Cathie Sudlow, Tim Chico, Jacqueline A. L. MacArthur, Aiden Doherty
Summary: The use of data from smartphones and wearable devices has great potential for population health research, but the uptake of large-scale mobile health research has not met expectations. Digital person-generated health data are crucial for answering research questions, but there are challenges such as data inequality, selection bias, data collection tools, data harmonization, time series data analysis, and patient and public involvement. Establishing an interdisciplinary community can transform people's lives for the better.
JOURNAL OF MEDICAL INTERNET RESEARCH
(2023)
Review
Chemistry, Analytical
Muhammad Ali Shiwani, Timothy J. A. Chico, Fabio Ciravegna, Lyudmila Mihaylova
Summary: Cardiovascular diseases cause 18 million deaths each year. Current assessment of a patient's health only occurs during infrequent clinical visits, providing limited information about their daily life health. Advances in mobile health technologies, such as wearable devices, allow for continuous monitoring of health indicators and mobility. Longitudinal, clinically relevant measurements obtained through these technologies can improve prevention, detection, and treatment of cardiovascular diseases. This review discusses the advantages and disadvantages of different methods for monitoring cardiovascular disease patients during daily life, including physical activity, indoor home, and physiological parameter monitoring.
Article
Biochemical Research Methods
Rory M. Power, Anjalie Schlaeppi, Jan Huisken
Summary: We propose a scheme for multi-directional illumination in selective plane illumination microscopy (SPIM) using a single galvanometric scanning mirror. This scheme allows light sheets to be delivered from two opposed directions and pivoted about their center for effective stripe artifact suppression. Compared with comparable schemes, this approach results in a smaller instrument footprint and reduced expense. It also maintains the lowest rates of photodamage and is suitable for high-speed imaging applications.
BIOMEDICAL OPTICS EXPRESS
(2023)
Review
Biology
Stephan Daetwyler, Reto Paul Fiolka
Summary: This review discusses the future of fluorescence imaging with light sheets and the emergence of adaptive and smart imaging schemes. Light-sheet fluorescence microscopy has greatly transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods. The review explores current and future developments in light-sheet fluorescence microscopy, including smart and adaptive imaging schemes, image restoration techniques, and the potential for multi-modal imaging with high throughput. It predicts that light-sheet microscopy will play a crucial role in biomedical and clinical imaging in the future.
COMMUNICATIONS BIOLOGY
(2023)
Editorial Material
Sport Sciences
Timothy James Chico, Emmanuel Stamatakis, Fabio Ciravegna, Jessilyn Dunn, Simon Redwood, Rasha Al-lamee, Reecha Sofat, Jason Gill
BRITISH JOURNAL OF SPORTS MEDICINE
(2023)
Editorial Material
Sport Sciences
Emmanuel Stamatakis, Matthew Ahmadi, Marie H. Murphy, Timothy James Chico, Karen Milton, Borja Del Pozo Cruz, Peter T. Katzmarzyk, I-Min Lee, Jason Gill
BRITISH JOURNAL OF SPORTS MEDICINE
(2023)
Editorial Material
Sport Sciences
Jason M. R. Gill, Timothy J. Chico, Aiden Doherty, Jessilyn Dunn, Ulf Ekelund, Peter T. Katzmarzyk, Karen Milton, Marie H. Murphy, Emmanuel Stamatakis
BRITISH JOURNAL OF SPORTS MEDICINE
(2023)
Article
Chemistry, Analytical
Peter D. Winter, Timothy J. A. Chico
Summary: A digital twin is a virtual representation of a complex system, updated with data from its real counterpart. They are utilized in various industries, including medicine, to improve healthcare and prevention. However, the implementation of digital twins faces technical, scientific, social, and ethical challenges that need to be addressed.
Article
Medicine, General & Internal
Kamilla Abdullayev, Timothy J. A. Chico, Matthew Manktelow, Oliver Buckley, Joan Condell, Richard J. Van Arkel, Vanessa Diaz, Faith Matcham
Summary: Cardiovascular diseases are prevalent in the UK, but the quality of care is declining due to accessibility and resource issues. The implementation of digital technologies in cardiovascular care has the potential to relieve the burden on the NHS, but it is important to consider the perspectives of both service users and clinicians.