Article
Biochemistry & Molecular Biology
Melis D. Arslanhan, Navin Rauniyar, John R. Yates, Elif N. Firat-Karalar
Summary: AURKA is a conserved kinase in cell processes and is frequently overexpressed in human cancers. It was found to have extensive interactions with centriolar satellites, regulating its activity and localization. Loss of satellites leads to AURKA activation at the basal body and causes ciliogenesis defects.
Article
Biology
Emma A. Hall, Dhivya Kumar, Suzanna L. Prosser, Patricia L. Yeyati, Vicente Herranz-Perez, Jose Manuel Garcia-Verdugo, Lorraine Rose, Lisa McKie, Daniel O. Dodd, Peter A. Tennant, Roly Megaw, Laura C. Murphy, Marisa F. Ferreira, Graeme Grimes, Lucy Williams, Tooba Quidwai, Laurence Pelletier, Jeremy F. Reiter, Pleasantine Mill
Summary: By generating mice lacking PCM1, this study reveals the important role of PCM1 and centriolar satellites in ciliogenesis, specifically in motile cilia on ependymal cells. The findings demonstrate that PCM1 is involved in efficient protein trafficking to and from centrioles, including the removal of CP110 and CEP97 to initiate ciliogenesis, and the threshold for ciliogenesis differs between cell types.
Review
Cell Biology
Clotilde C. N. Renaud, Nicolas Bidere
Summary: Centriolar satellites are small granules that revolve around the centrosome, and recent research has uncovered their complex nature and diverse roles in cellular processes beyond centrosome and primary cilia formation. Post-translational modifications like phosphorylation and ubiquitination play a crucial role in shaping the composition and functions of these structures.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Obstetrics & Gynecology
Philip Xie, Olena M. Kocur, Stephanie Cheung, Lily Ng, David F. Albertini, Zev Rosenwaks, Gianpiero D. Palermo
Summary: The sperm centrosome, an important organelle in fertilization and embryonic development, plays a crucial role in mitotic spindle formation, chromosome segregation, and syngamy. Abnormalities of the sperm centrosome can lead to abnormal embryonic development, pregnancy loss, and chromosomal instability. Understanding the molecular composition, regulation, and function of the sperm centrosome is vital for elucidating the mechanisms of successful fertilization and embryonic development, as well as addressing infertility and developmental disorders associated with centrosomal defects.
FERTILITY AND STERILITY
(2023)
Article
Biology
Dandan Ma, Fulin Wang, Rongyi Wang, Yingchun Hu, Zhiquan Chen, Ning Huang, Yonglu Tian, Yuqing Xia, Junlin Teng, Jianguo Chen
Summary: In this study, new components of the subdistal appendage (SDA), alpha-taxilin and gamma-taxilin, were identified and found to form a complex and play a role in the hierarchical assembly of the SDA. Additionally, the molecular composition and functional understanding of the SDA were revealed.
Article
Biochemistry & Molecular Biology
Matteo Burigotto, Alessia Mattivi, Daniele Migliorati, Giovanni Magnani, Chiara Valentini, Michela Roccuzzo, Martin Offterdinger, Massimo Pizzato, Alexander Schmidt, Andreas Villunger, Stefano Maffini, Luca L. Fava
Summary: This study demonstrates that PIDD1 is recruited to centrosomes by ANKRD26, triggering PIDDosome activation and influencing cell fate. Supernumerary centrosomes resulting from centrosome amplification can activate PIDDosome, and this activation can also occur independently of centrosome amplification in the context of DNA damage.
Review
Cell Biology
Robert Mahen
Summary: The article discusses the various roles and assembly pathways of rootlets in cellular function and structure, pointing out that the structure and function of rootlets still require further investigation.
JOURNAL OF CELL SCIENCE
(2021)
Editorial Material
Cell Biology
Ashari R. Kannangara, Joshua L. Andersen
Summary: This study used BioID and quantitative LC-MS/MS techniques to investigate the proximity interactome of ATG9A, revealing interactions with protein complexes involved in protein trafficking and autophagy. Additionally, an unexpected interaction between ATG9A and the ULK1-independent ATG13-ATG101 dimer was discovered, which promotes autophagy in fed cells.
Article
Biochemistry & Molecular Biology
Shuwei Xie, Naava Naslavsky, Steve Caplan
Summary: Primary cilia are sensory organelles that coordinate diverse signaling pathways, controlling development and homeostasis. In this study, we found that EHD1 regulates the ubiquitination of CP110, a protein required for ciliogenesis, and identified HERC2 and MIB1 as E3 ubiquitin ligases that interact with and ubiquitinate CP110. We also discovered that HERC2 is necessary for ciliogenesis and localizes to centriolar satellites, which play a role in ciliogenesis regulation. Our findings highlight a mechanism in which EHD1 controls the movement of centriolar satellites and HERC2 to the mother centriole during ciliogenesis, promoting CP110 ubiquitination and degradation.
Article
Biology
Fernando R. Balestra, Andres Dominguez-Calvo, Benita Wolf, Coralie Busso, Alizee Buff, Tessa Averink, Marita Lipsanen-Nyman, Pablo Huertas, Rosa M. Rios, Pierre Gonczy
Summary: The research suggests that TRIM37 may prevent the formation of Cenpas by regulating the stability and solubility of Centrobin, thus maintaining genome integrity.
Article
Cell Biology
Camila de Castro Ferezin, Terry C. C. Lim Kam Sian, Yunjian Wu, Xiuquan Ma, Anderly C. Chueeh, Cheng Huang, Ralf B. Schittenhelm, Jorg Kobarg, Roger J. Daly
Summary: In this study, we found that NEK5 has diverse effects on signaling pathways and biological processes in breast epithelial cells, and may play an important role in the development and progression of breast cancer.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Anastassia L. Kantsadi, Georgios N. Hatzopoulos, Pierre Gonczy, Ioannis Vakonakis
Summary: Centrioles are eukaryotic organelles that play important roles in cellular processes like cilia and flagella formation, microtubule network organization, and mitotic spindle assembly. This research reveals that the asymmetric interaction mediated by the coiled-coil domain of SAS-6 is crucial for the assembly and polarity of the centriolar central scaffold, the cartwheel. Disrupting this asymmetric interaction impairs the stacking of SAS-6 rings. These findings provide new insights into the assembly of centrioles and cell division.
Article
Biochemical Research Methods
Camila M. Aprosoff, Boris J. A. Dyakov, Vivian H. W. Cheung, Cassandra J. Wong, Mikaela Palandra, Anne-Claude Gingras, Haley D. M. Wyatt
Summary: The DNA repair scaffold SLX4 plays a pivotal role in maintaining genome stability, particularly in homologous recombination. Germline mutations in SLX4 are associated with Fanconi anemia, chromosome instability, and cancer susceptibility. By using BioID and AP-MS techniques, we identified 221 unique high-confidence interactors of SLX4, most of which are novel SLX4-binding proteins. This comprehensive SLX4 interactome provides insights into its role in DNA repair and reveals new cellular processes that may involve SLX4.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Cell Biology
Bailey L. McCurdy, Cayla E. Jewett, Alexander J. Stemm-Wolf, Huy Nguyen Duc, Molishree Joshi, Joaquin M. Espinosa, Rytis Prekeris, Chad G. Pearson
Summary: Trisomy 21 leads to increased PCNT protein, which disrupts primary cilia formation and signaling. Enlarged cytoplasmic puncta of PCNT affect the density and stability of the microtubule network, leading to defects in cilia. Decreasing PCNT can alleviate these defects in trisomy 21 cells.
MOLECULAR BIOLOGY OF THE CELL
(2022)
Article
Developmental Biology
Sven Willekers, Federico Tessadori, Babet van der Vaart, Heiko H. Henning, Riccardo Stucchi, Maarten Altelaar, Bernard A. J. Roelen, Anna Akhmanova, Jeroen Bakkers
Summary: In zebrafish embryos, Cfap53 is involved in the formation of the zygotic MTOC and aids in the proper assembly of the mitotic spindle.
Review
Oncology
Rodrigo Borrega, Carlos Araujo, Nadia Aguiam, Fernando Magro, Joao Eurico Fonseca, Silvio Danese, Joao Lopes, Joao Goncalves
Summary: This study identified significant variability in the reported immunogenicity rates of adalimumab across different studies, with a noticeable increase over time. The type of assay used to detect anti-adalimumab antibodies, as well as the study type and exposure time, were found to be associated with the immunogenicity rates. Factors contributing to the temporal increase in immunogenicity rates require further investigation in future studies.
Review
Rheumatology
Vibeke Strand, Joao Goncalves, John D. Isaacs
Summary: Biologic agents have become crucial in treating inflammatory rheumatic diseases, but their immunogenicity can impact treatment response and adverse events. Immunogenicity testing is becoming part of routine clinical management in some fields, with the cost-effectiveness in rheumatology still being studied.
NATURE REVIEWS RHEUMATOLOGY
(2021)
Article
Chemistry, Medicinal
Iris Cadima-Couto, Alexandra Tauzin, Joao M. Freire, Tiago N. Figueira, Ruben D. M. Silva, Clara Perez-Peinado, Catarina Cunha-Santos, Ines Bartolo, Nuno Taveira, Lurdes Gano, Joao D. G. Correia, Joao Goncalves, Fabrizio Mammano, David Andreu, Miguel A. R. B. Castanho, Ana Salome Veiga
Summary: The anti-HIV-1 peptide pepRF1, derived from the Dengue virus capsid protein, shows promising inhibitory effects with a potential therapeutic window higher than 53,000. It specifically targets CXCR4-tropic strains, preventing viral entry into target cells by acting as an antagonist of the viral coreceptor CXCR4. Compared to T20, a peptide-based HIV-1 entry inhibitor, pepRF1 is more effective and can potentially be used alone or in combination with other anti-HIV drugs, as well as a novel therapeutic strategy for other CXCR4-related diseases.
ACS INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Raksha Devi, Laurence Pelletier, Suzanna L. Prosser
Summary: The centrosome, primary cilium, and centriolar satellites play critical roles in cellular processes and are linked to a variety of human diseases. Understanding the molecular mechanisms underlying these diseases requires studying the biology of these organelles. Technological breakthroughs have provided unprecedented spatial and temporal resolution in dissecting the molecular players of these enigmatic organelles.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kwangjin Park, Chunmei Li, Sofia Tsiropoulou, Joao Goncalves, Christine Kondratev, Laurence Pelletier, Oliver E. Blacque, Michel R. Leroux
Summary: Cilia play essential roles in cell motility, fluid flow, and signaling, and are divided into subcompartments with variable structures and functions. CDKL-1, a kinase related to the human CDKL family, controls the length of a specific ciliary subdomain and influences cilium-dependent processes. Overall, cilium length is regulated by various pathways and CDKL-1 is crucial for the construction of a specific ciliary compartment and contributes to development and sensory physiology.
Article
Cell Biology
Yi Xin (Iris) Tu, Andrew M. Sydor, Etienne Coyaud, Estelle M. N. Laurent, Diana Dyer, Nora Mellouk, Jonathan St-Germain, Robert M. Vernon, Julie D. Forman-Kay, Taoyingnan Li, Rong Hua, Kexin Zhao, Neale D. Ridgway, Peter K. Kim, Brian Raught, John H. Brumell
Summary: This study identified a network of interactions among core human macroautophagy proteins using proximity-dependent biotin identification, highlighting the critical role of OSBPL family members in the macroautophagy process.
Article
Cell Biology
Bing-Ru Yan, Taoyingnan Li, Etienne Coyaud, Estelle M. N. Laurent, Jonathan St-Germain, Yuhuan Zhou, Peter K. Kim, Brian Raught, John H. Brumell
Summary: The protein C5orf51 plays a crucial role in regulating the activity of RAB7A during mitophagy, facilitating the shuttle of RAB7A between late endosomes and mitochondria. Depletion of C5orf51 leads to compromised localization of RAB7A on depolarized mitochondria, degradation by the proteasome, and inhibition of ATG9A recruitment to depolarized mitochondria.
Article
Chemistry, Analytical
Ines Iria, Ruben R. G. Soares, Eduardo J. S. Bras, Virginia Chu, Joao Goncalves, Joao P. Conde
Summary: Inflammatory bowel disease (IBD) is a chronic inflammatory disorder affecting over 6.8 million people worldwide. Biological therapy, specifically anti-TNF-alpha antibodies, is used in severe cases. The development of a microfluidic biosensor for quantifying therapeutic antibodies in IBD patient samples shows promising results for improving therapy scheduling and efficacy.
Article
Biochemistry & Molecular Biology
Melissa Iazzi, Audrey Astori, Jonathan St-Germain, Brian Raught, Gagan D. Gupta
Summary: Deletion of phenylalanine 508 (increment F508) of the CFTR protein is the main cause of CF. This study analyzed the CFTR and increment F508-CFTR interactomes using BioID, and identified known and novel protein interactions. The increment F508 interactome showed significant differences compared to the wild type, with alterations in membrane trafficking and cellular stress functions. Drug therapy also affected the interactome, and depletion of specific interactors impacted CFTR functionality.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Correction
Multidisciplinary Sciences
Christopher D. Go, James D. R. Knight, Archita Rajasekharan, Bhavisha Rathod, Geoffrey G. Hesketh, Kento T. Abe, Ji-Young Youn, Payman Samavarchi-Tehrani, Hui Zhang, Lucie Y. Zhu, Evelyn Popiel, Jean-Philippe Lambert, Etienne Coyaud, Sally W. T. Cheung, Dushyandi Rajendran, Cassandra J. Wong, Hana Antonicka, Laurence Pelletier, Alexander F. Palazzo, Eric A. Shoubridge, Brian Raught, Anne-Claude Gingras
Article
Cell Biology
Suzanna L. Prosser, Johnny Tkach, Ladan Gheiratmand, Jaeyoun Kim, Brian Raught, Ciaran G. Morrison, Laurence Pelletier
Summary: In this study, the researchers found that centriolar satellite and centrosomal proteins are crucial for the formation of aggresomes and the aggregation of disease-relevant proteins. They showed that satellites are required for the growth of aggresomal structure and this growth depends on the centrosomal proteins CP110, CEP97, and CEP290.
NATURE CELL BIOLOGY
(2022)
Article
Clinical Neurology
Robert Siddaway, Laura Canty, Sanja Pajovic, Scott Milos, Etienne Coyaud, Stefanie-Grace Sbergio, Arun Kumaran Vadivel Anguraj, Evan Lubanszky, Hwa Young Yun, Alessia Portante, Sheyenne Carette, Cunjie Zhang, Michael F. Moran, Brian Raught, Eric I. Campos, Cynthia Hawkins
Summary: Histone H3 mutations at amino acids 27 (H3K27M) and 34 (H3G34R) drive pediatric-type high-grade glioma (pHGG) by disrupting chromatin modifications and altering cellular processes. H3K9 methylation plays a crucial role in regulating the effects of these mutations and could be a potential therapeutic target.
ACTA NEUROPATHOLOGICA
(2022)
Article
Biochemical Research Methods
Robert Siddaway, Scott Milos, Etienne Coyaud, Hwa Young Yun, Shahir M. Morcos, Sanja Pajovic, Eric I. Campos, Brian Raught, Cynthia Hawkins
Summary: In this study, unbiased quantitative interactomes for histone H3 variants H3.1 and H3.3 were reported based on BioID proximity labeling, revealing a significant number of new histone-associated proteins and important biological nuances not captured by traditional biochemical means.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Review
Pharmacology & Pharmacy
Zvonimir Petric, Joao Goncalves, Paulo Paixao
Summary: Monoclonal antibodies have revolutionized the treatment of chronic inflammatory diseases, but they face challenges related to their immunogenicity, effectiveness, and safety. Biosimilars have emerged as a cost-effective alternative and have been shown to be as safe and effective as the originator drugs in treating IBD patients.
Review
Biochemistry & Molecular Biology
Melissa Iazzi, Sara Sadeghi, Gagan D. Gupta
Summary: The aim of this review article is to summarize the recent contributions of proteomic studies to CFTR biology and provide a resource for future research. The focus is on CFTR's interaction network at the cell surface, creating a CFTR 'surfaceome', and highlighting potential biomarkers and drug targets for CF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)