Investigation on antimicrobial agents of the terrestrial Streptomyces sp BCC71188

The terrestrial actinomycete strain BCC71188 was identified as Streptomyces by its morphology (having spiral chain spore on the aerial mycelium), chemotaxonomy (containing LL-diaminopimelic acid in the cell wall), and 16S rRNA gene sequence analysis [showing high similarity values compared with Streptomyces samsunensis M1463(T) (99.85 %) and Streptomyces malaysiensis NBRC 16446(T) (99.40 %)]. The crude extract exhibited antimalarial against Plasmodium falciparum (IC50 0.19 mu g/ml), anti-TB against Mycobacterial tuberculosis (MIC 6.25 mu g/ml), and antibacterial against Bacillus cereus (MIC 1.56 mu g/ml) activities. Therefore, chemical investigation was conducted by employing bioassay-guided method and led to the isolation of 19 compounds including two cyclic peptides (1-2), five macrolides (3-7), new naphthoquinone (8), nahuoic acid C (9), geldanamycin derivatives (10-13), cyclooctatin (14), germicidins A (15) and C (16), actinoramide A (17), abierixin, and 29-O-methylabierixin. These isolated compounds were evaluated for antimicrobial activity, such as antimalarial, anti-TB, and antibacterial activities, and for cytotoxicity against both cancerous (MCF-7, KB, NCI-H187) and non-cancerous (Vero) cells. Compounds 1-7, 10-14 exhibited antimalarial (IC50 0.22-7.14 mu g/ml), and elaiophylin analogs (4-6) displayed anti-TB (MIC 0.78-12.00 mu g/ml) and B. cereus (MIC 0.78-3.13 mu g/ml) activities. Compounds 1, 2, 14, and abierixin displayed weak cytotoxicity, indicating a potential for antimicrobial agents.