Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts

The future of CRISPR technologies in agriculture

(2018) Caixia Gao   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis

(2017) Lindsey A Lonowski et al.   Nature Protocols

High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9

(2017) Javier Gil-Humanes et al.   PLANT JOURNAL

Defining and improving the genome-wide specificities of CRISPR–Cas9 nucleases

(2016) Shengdar Q. Tsai et al.   NATURE REVIEWS GENETICS

Transient Glyco-Engineering to Produce Recombinant IgA1 with Defined N- and O-Glycans in Plants

(2016) Martina Dicker et al.   Frontiers in Plant Science

Transient Expression of Tetrameric Recombinant Human Butyrylcholinesterase in Nicotiana benthamiana

(2016) Salem Alkanaimsh et al.   Frontiers in Plant Science

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

Fast and sensitive detection of indels induced by precise gene targeting

(2015) Zhang Yang et al.   NUCLEIC ACIDS RESEARCH

Improving cold storage and processing traits in potato through targeted gene knockout

(2015) Benjamin M. Clasen et al.   PLANT BIOTECHNOLOGY JOURNAL

When plant virology metAgrobacterium: the rise of the deconstructed clones

(2015) Hadrien Peyret et al.   PLANT BIOTECHNOLOGY JOURNAL

Within leaf variation is the largest source of variation in agroinfiltration of Nicotiana benthamiana

(2015) Hany Bashandy et al.   Plant Methods

Auxin binding protein 1 (ABP1) is not required for either auxin signaling orArabidopsisdevelopment

(2015) Yangbin Gao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing

(2015) Kangquan Yin et al.   Scientific Reports

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

Oligomerization status influences subcellular deposition and glycosylation of recombinant butyrylcholinesterase inNicotiana benthamiana

(2014) Jeannine D. Schneider et al.   PLANT BIOTECHNOLOGY JOURNAL

Biochemical composition of haemagglutinin-based influenza virus-like particle vaccine produced by transient expression in tobacco plants

(2014) François Le Mauff et al.   PLANT BIOTECHNOLOGY JOURNAL

DNA Replicons for Plant Genome Engineering

(2014) N. J. Baltes et al.   PLANT CELL

Plant regeneration from leaf-derived protoplasts within the Daucus genus: effect of different conditions in alginate embedding and phytosulfokine application

(2014) Katarzyna Maćkowska et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis

(2014) Z. Feng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Application of the CRISPR–Cas System for Efficient Genome Engineering in Plants

(2013) Yanfei Mao et al.   Molecular Plant

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

A Draft Genome Sequence of Nicotiana benthamiana to Enhance Molecular Plant-Microbe Biology Research

(2012) Aureliano Bombarely et al.   MOLECULAR PLANT-MICROBE INTERACTIONS

Tiptoeing around transgenics

(2012) Emily Waltz   NATURE BIOTECHNOLOGY

Physical methods for genetic plant transformation

(2012) Ana Leonor Rivera et al.   Physics of Life Reviews

Monoclonal tobacco cell lines with enhanced recombinant protein yields can be generated from heterogeneous cell suspension cultures by flow sorting

(2012) Janina Kirchhoff et al.   PLANT BIOTECHNOLOGY JOURNAL

Transcription Activator-Like Effector Nucleases Enable Efficient Plant Genome Engineering

(2012) Y. Zhang et al.   PLANT PHYSIOLOGY

A Novel Method of Transgene Delivery into Triticale Plants Using the Agrobacterium Transferred DNA-Derived Nano-Complex

(2012) A. Ziemienowicz et al.   PLANT PHYSIOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

O-Glycosylated Cell Wall Proteins Are Essential in Root Hair Growth

(2011) S. M. Velasquez et al.   SCIENCE

A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering

(2010) Morten HH Nørholm   BMC BIOTECHNOLOGY

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

Fluorescence Activated Cell Sorting of Plant Protoplasts

(2010) Bastiaan O. R. Bargmann et al.   Jove-Journal of Visualized Experiments

High-frequency modification of plant genes using engineered zinc-finger nucleases

(2009) Jeffrey A. Townsend et al.   NATURE

High level protein expression in plants through the use of a novel autonomously replicating geminivirus shuttle vector

(2009) Guy L. Regnard et al.   PLANT BIOTECHNOLOGY JOURNAL

Extremely High-Level and Rapid Transient Protein Production in Plants without the Use of Viral Replication

(2008) F. Sainsbury et al.   PLANT PHYSIOLOGY