Article
Plant Sciences
Adeola Folasade Ehigie, Peng Wei, Taotao Wei, Xiyun Yan, Olufunso O. Olorunsogo, Fiyinfoluwa Demilade Ojeniyi, Leonard Ona Ehigie
Summary: The study aimed to investigate the mechanism of cell death in breast and lung cancer cell lines treated with fractions derived from bitter melon extract. The results showed that the fractions reduced mitochondrial membrane potential and intracellular ATP levels, while increasing reactive oxygen species levels. However, they did not activate caspase-3 or release cytochrome c in the cancer cells, suggesting a different mechanism of cell death induction.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Javier Florido, Cesar Rodriguez-Santana, Laura Martinez-Ruiz, Alba Lopez-Rodriguez, Dario Acuna-Castroviejo, Iryna Rusanova, Germaine Escames
Summary: ROS are highly reactive molecules that regulate important signaling pathways. Tumor cells have altered redox balance compared to normal cells, making them susceptible to anti-tumoral therapy targeting ROS levels and antioxidant system capacity. Melatonin has potential as an innovative cancer treatment, but its mechanism of action remains unclear, limiting its clinical applications.
Article
Biochemistry & Molecular Biology
James H. Schofield, Zachary T. Schafer
Summary: The relationship between mitophagy and ROS production is complex and not fully understood. This review discusses mtROS generation and their detrimental effects on cellular viability, along with the cellular defense mechanisms against oxidative stress. Furthermore, the prominent mechanisms governing mitophagy induction that bear on oxidative stress are explored.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Engineering, Environmental
Guoliang Chen, Mingxing Wang, Panpan Zhu, Guixue Wang, Tingzhang Hu
Summary: Pyraoxystrobin (SYP-3343), a newly-invented strobilurin fungicide, exhibits high toxicity to aquatic organisms, particularly in zebrafish embryos, through affecting cell cycle, mitochondrial membrane potential, and reactive oxygen species generation. This toxicity is dose- and time-dependent, inducing apoptosis in zebrafish embryos.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Review
Immunology
Miranda D. Chavez, Hubert M. Tse
Summary: Mitochondrial dysfunction and oxidative stress are associated with T cell-mediated autoimmune diseases, and targeting metabolic pathways can inhibit autoreactive T cell activation. Increasing the requirements of Tregs for ROS and oxidative phosphorylation can promote self-tolerance and inhibit the activity of autoreactive T cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chou-Yuan Ko, Po-Chang Shih, Po-Wei Huang, Yi-Hsin Lee, Yen-Fu Chen, Ming-Hong Tai, Chi-Hao Liu, Zhi-Hong Wen, Hsiao-Mei Kuo
Summary: Sinularin was found to effectively inhibit the growth of hepatocellular carcinoma SK-HEP-1 cells at a concentration of 10 μM, inducing DNA fragmentation and apoptosis, as well as affecting cell migration and colony formation. Additionally, it altered the expression levels of various proteins related to cell signaling and metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Andrew P. Bischer, Timothy M. Baran, Andrew P. Wojtovich
Summary: Environmental surveillance-mediated behavior in Caenorhabditis elegans integrates multiple cues and is coordinated by neurons through signaling cascades. Despite lacking eyes, C. elegans is able to perceive and react to the color blue. This study explains this color perception by showing that internally-generated reactive oxygen species (ROS), in response to light, add to exogenous sources of ROS. Multiple sub-threshold sources of ROS are integrated to coordinate behavioral responses with internal cues. Blue light affects C. elegans behavior through ROS generation by endogenous flavins, mediated by the neuronal gustatory photoreceptor like protein, LITE-1. Overall, this study demonstrates that ROS and LITE-1 play central roles in C. elegans foraging behavior through integration of multiple inputs, including light.
Article
Immunology
Nan-Nan Liang, Ying Zhao, Yue-Yue Guo, Zhi-Hui Zhang, Lan Gao, De-Xin Yu, De-Xiang Xu, Shen Xu
Summary: This study found that mitochondria-derived reactive oxygen species (ROS) contribute to renal cell ferroptosis during lipopolysaccharide (LPS)-induced acute kidney injury (AKI). Inhibition of ferroptosis and scavenging of mitochondrial ROS with specific inhibitors, ferrostatin-1 and MitoQ, respectively, attenuated renal lipid peroxidation, ferroptosis-characteristic mitochondrial damage, and renal cell death. These findings suggest that mitochondria-targeted antioxidants may be potential therapeutic agents for sepsis-induced AKI.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Anatomy & Morphology
Shao-Peng Lin, Jingyi Bu, Shan Ye, Qiangda Xie, Jue-Xian Wei, Xiaofang Yin, Fen Mei, Pei-Yi Lin, Xiao-Hui Chen
Summary: This study investigated whether AMPK-induced glucose metabolic and mitochondrial dysfunction were related to glutamate-dependent ROS injury of neurons. The results showed that AMPK phosphorylation promoted glucose uptake, enhanced oxygen consumption rate and intracellular ROS levels. Inhibition of AMPK mitigated glutamate-induced cellular toxicity. These findings suggest that AMPK phosphorylation may be a potential target for treating glutamate-induced neurotoxicity.
Article
Cell Biology
Yanhong Wang, Xinyu Mang, Xuran Li, Zhengyu Cai, Fei Tan
Summary: This study investigated the effect and mechanism of cold atmospheric plasma (CAP) on the proliferation and apoptosis of lung cancer and colon cancer cells. The results showed that CAP treatment inhibited tumor cell proliferation and induced apoptosis through the activation of the mitochondrial pathway.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hiromu Ito, Hiromi Kurokawa, Hirofumi Matsui
Summary: Mitochondria, essential organelles for energy production in eukaryotes, generate reactive oxygen species during the process which play pivotal roles in cell signaling and iron homeostasis regulation. The regulation of iron transportation, involving proteins like HCP1, DMT1, and mitoferrin, is increasingly understood in relation to mitochondrial reactive oxygen species and diseases.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Andrey Y. Vinokurov, Olga A. Stelmashuk, Polina A. Ukolova, Evgeny A. Zherebtsov, Andrey Y. Abramov
Summary: The brain produces various reactive oxygen species at different rates in different brain regions, with the brain stem and cerebellum being more sensitive to oxidative stress. Mitochondrial ROS has minor implications to total ROS production, and levels of lipid peroxidation and GSH vary across brain regions.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Medicine, Research & Experimental
Zhi-Hong Wen, Hsiao-Mei Kuo, Po-Chang Shih, Ling-Chen Hsu, Jimmy Ming-Jung Chuang, Nan-Fu Chen, Hsi-Wen Sun, Hsin-Tzu Liu, Chun-Sung Sung, Wu-Fu Chen
Summary: This study evaluated the effects of isoaaptamine and aaptamine on cell viability in glioblastoma multiforme (GBM) cell lines. The results showed that isoaaptamine was more potent than aaptamine in these cell lines, with GBM 8401 being the most sensitive. The study also found that isoaaptamine could induce apoptosis, increase oxidative stress, disrupt mitochondrial function, and trigger autophagy in GBM cells.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Immunology
Marilyne Lavergne, Maria Andrea Hernandez-Castaneda, Pierre-Yves Mantel, Denis Martinvalet, Michael Walch
Summary: Cell-mediated cytotoxicity is a crucial immune defense mechanism against viral, bacterial, or parasitic infections, where killer lymphocytes release cytotoxic granules containing perforin, granulysin, and granzymes to attack infected cells. Granzymes can induce host cell apoptosis or exert non-cytolytic antimicrobial activities by degrading microbial substrates or targeting factors mediating microbial virulence. Reactive oxygen species play a key role in the antimicrobial mechanisms exerted by the granzymes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Michael O. Isei, Don Stevens, Collins Kamunde
Summary: The research found that heart mitochondria show different responses to copper and thermal stress, and the consumption capacity of H2O2 is influenced by the type of substrate, temperature, and copper. The NADPH-dependent peroxidase system is the centerpiece of ROS scavenging in heart mitochondria, and copper has the ability to inhibit specific mitochondrial ROS scavenging pathways.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Immunology
Prarthana J. Dalal, David P. Sullivan, Evan W. Weber, David B. Sacks, Matthias Gunzer, Isabella M. Grumbach, Joan Heller Brown, William A. Muller
Summary: The research highlights the important roles of endothelial CaM and CaMKIIδ in transducing specific calcium signals required for TEM, which interferes with the delivery of the lateral border recycling compartment (LBRC) and reduces TEM.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Ophthalmology
Michelle R. Tamplin, Kimberly A. Broadhurst, Anthony H. Vitale, Ryuya Hashimoto, Randy H. Kardon, Isabella M. Grumbach
Summary: The study investigated the use of LSFG for blood flow analysis in mice during and after angiotensin-II infusion, finding that hypertension induced by angiotensin-II leads to reduced blood flow, increased vascular resistance, and decreased blood flow area in retinal vessels. These effects reversed post-infusion for 2 weeks, but persisted up to 6 weeks post-infusion in mice administered with angiotensin-II for 4 weeks. The study highlights the potential utility of retinal LSFG for translational studies on hypertension-induced microvascular effects.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Aarti Asnani, Javid J. Moslehi, Bishow B. Adhikari, Alan H. Baik, Andreas M. Beyer, Rudolf A. de Boer, Alessandra Ghigo, Isabella M. Grumbach, Salvia Jain, Han Zhu
Summary: Cardio-oncology has emerged as a new field focusing on the cardiovascular toxicity associated with cancer therapy, aiming to understand the underlying molecular mechanisms to improve patient outcomes. The intersection of cancer and heart disease has led to the discovery of genetic and environmental risk factors predisposing to both conditions. Preclinical models serve as the foundation for mechanistic studies in the development of biomarkers and cardioprotective therapies.
CIRCULATION RESEARCH
(2021)
Editorial Material
Hematology
Hanrui Zhang, Zhen Bouman Chen, Gabrielle Fredman, Delphine Gomez, Isabella M. Grumbach, Ngan F. Huang, Patricia Kim Phuong Nguyen, Mireille Ouimet, Nadia R. Sutton, Elena Aikawa
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Biochemical Research Methods
Kuo-Kuang Wen, Stephen Roy, Isabella M. Grumbach, Meng Wu
Summary: In an attempt to find rescuing small molecules for radiation damage, researchers developed a 96-well microplate-based in vitro assay for high-throughput measurement. They observed radiation-induced cell damage and rescue effects on endothelial cells under various conditions using live-cell imaging and functional assays. Despite not moving forward with the pilot screening, lessons learned from this failed assay development were shared.
Article
Cardiac & Cardiovascular Systems
Zhiyong Zhu, Zhan Gao, Biyi Chen, Duane D. Hall, Rachel Minerath, Olha Koval, Ana Sierra, Ekaterina Subbotina, Xiaoyi Zhu, Young-Rae Kim, Jun Yang, Isabella Grumbach, Kaikobad Irani, Chad Grueter, Long-Sheng Song, Denice M. Hodgson-Zingman, Leonid Zingman
Summary: The study demonstrates that delivering a exercise-similar heart rate pattern through atrial pacing while maintaining intracardiac synchrony can induce cardiac conditioning and enhance resistance to ischemic stress. This suggests that the heart rate pattern serves as a conditioning signal and has the potential to be repurposed for cardioprotection.
Correction
Biochemistry & Molecular Biology
Stephen J. Roy, Olha M. Koval, Sara C. Sebag, Karima Ait-Aissa, Bryan G. Allen, Douglas R. Spitz, Isabella M. Grumbach
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Correction
Oncology
Sara C. Sebag, Olha M. Koval, John D. Paschke, Christopher J. Winters, Alejandro P. Comellas, Isabella M. Grumbach
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Ophthalmology
Michelle R. Tamplin, Kimberly A. Broadhurst, Anthony H. Vitale, Ryuya Hashimoto, Randy H. Kardon, Isabella M. Grumbach
Summary: Alterations in neurovascular coupling have been associated with various ocular, cerebral, and systemic vascular disorders. A new method for quantifying light-flicker induced hyperemia in mouse retina was presented using laser speckle flowgraphy (LSFG). This method showed low variability over multiple imaging sessions, indicating its potential application in serial studies of neurovascular coupling.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Hematology
Karima Ait-Aissa, Olha M. Koval, Nathanial R. Lindsey, Isabella M. Grumbach
Summary: Radiation therapy increases the risk of atherosclerotic vascular disease, but the mechanisms involved are not well understood. This study shows that radiation induces DNA damage in endothelial cells, particularly in mitochondrial DNA, leading to mitochondrial dysfunction and endothelial dysfunction. Targeting the mitochondrial calcium uniporter and mitochondrial reactive oxygen species could help mitigate radiation-induced vascular disease.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Ophthalmology
Michelle R. Tamplin, Jui-Kai Wang, Anthony H. Vitale, Ryuya Hashimoto, Mona K. Garvin, Elaine M. Binkley, Daniel E. Hyer, John M. Buatti, H. Culver Boldt, Randy H. Kardon, Isabella M. Grumbach
Summary: In this study, reductions in retinal and choroidal blood flow were detected using laser speckle flowgraphy after I-125-plaque brachytherapy for uveal melanoma, suggesting that decreased blood flow may be an early indicator of microangiographic response to radiation therapy.
Article
Cardiac & Cardiovascular Systems
Jared M. McLendon, Xiaoming Zhang, Daniel S. Matasic, Mohit Kumar, Olha M. Koval, Isabella M. Grumbach, Sakthivel Sadayappan, Barry London, Ryan L. Boudreau
Summary: This study explores the dysregulation of Sorbs2 in adult-onset cardiomyopathies and heart failure. It demonstrates that Sorbs2 is crucial for maintaining structural integrity in cardiomyocytes and its loss leads to cardiac structural changes and contractile dysfunction. The study also identifies genetic variants associated with decreased Sorbs2 expression and human cardiac phenotypes.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Cardiac & Cardiovascular Systems
Celio Damacena de Angelis, Benney T. Endoni, Daniel Nuno, Kathryn Lamping, Johannes Ledolter, Olha M. Koval, Isabella M. Grumbach
Summary: This study investigated sex differences in vasoreactivity and the role of mitochondrial Ca2+ entry via the MCU in resistance arteries. Female mice exhibited reduced vasodilation compared to male mice in genetic models of endothelial MCU ablation. These differences were attributed to higher cytosolic Ca2+ transients and enhanced mitochondrial Ca2+ entry in female mice. Estradiol treatment in human aortic endothelial cells also increased cytosolic Ca2+ transients and mitochondrial Ca2+ entry. These findings suggest that differences in mitochondrial function, rather than MCU complex composition, contribute to the sex-specific differences in vasodilation.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Cardiac & Cardiovascular Systems
Emma Herbach, Michael A. O'Rorke, Ryan M. Carnahan, Bradley D. McDowell, Bryan Allen, Isabella Grumbach, Barry London, Brian J. Smith, Douglas R. Spitz, Aaron Seaman, Elizabeth A. Chrischilles
Summary: This study compared the cardiac outcomes of stage III non-small-cell lung cancer patients who received adjuvant or neoadjuvant chemotherapy versus chemo-radiation therapy. The results showed that adding radiation therapy to chemotherapy increased the risk of severe cardiac events, and patients who received chemo-radiation had a higher cancer severity.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Ophthalmology
Michelle R. Tamplin, Wenxiang Deng, Mona K. Garvin, Elaine M. Binkley, Daniel E. Hyer, John M. Buatti, Johannes Ledolter, H. Culver Boldt, Randy H. Kardon, Isabella M. Grumbach
Summary: Through quantitative methods, radiation microvasculopathy and visual field loss can be detected earlier after brachytherapy. During radiation therapy, radiation-induced ischemia appears to be a primary early manifestation of radiation retinopathy.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
