Article
Medicine, General & Internal
Kaori Takeshita, Ichiro Abe, Wataru Kameda, Kota Ishii, Yuya Fujita, Mai Nagata, Kentaro Ochi, Yuki Senda, Midori Koga, Tadachika Kudo, Yurika Hada, Kaoru Takase, Yusuke Morinaga, Miiko Ito, Makiko Abe, Kenichi Ishizawa, Kunihisa Kobayashi
Summary: Pituitary apoplexy is a rare syndrome that often leads to spontaneous bleeding or infarction in pituitary tumors or glands. This study found that pituitary apoplexy occurs most frequently in patients with nonfunctional pituitary adenomas and is not related to tumor size. However, it is associated with hormonal deficiencies, liver dysfunction, hyponatremia or hypochloremia, and dyslipidemia.
Article
Chemistry, Analytical
Wenhan Li, Xiaojun Yang, Tingting Bai, Junwen Xu, Zhuyin Qian, Yawen Li, Zhirui Guo, Yefei Zhu
Summary: Pancreatic cancer has a high mortality rate due to its insidious onset and lack of early clinical symptoms. This study presents a new method for detecting tumor-secreted extracellular vesicles (EVs) in serum, which could be a potential biomarker for pancreatic cancer. The method uses light-initiated chemiluminescent assay (LICA) with photosensitive beads and can rapidly distinguish pancreatic cancer patients by detecting EphA2-EVs in serum without purification.
ANALYTICAL METHODS
(2022)
Article
Medical Laboratory Technology
Yuan Ren, Ying Wang, Xinjie Bao, Ming Feng, Bing Xing, Wei Lian, Yong Yao, Renzhi Wang
Summary: Incomplete surgical resection of invasive non-functional pituitary adenomas (NFPAs) carries a risk of subsequent complications. This study examined biomarkers for determining the invasiveness of NFPAs and explored therapeutic targets. The results highlighted the role of MMP1 in tumor invasion and angiogenesis, with exosomes playing a regulatory role.
CLINICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Shan Chen, Tao Jiang, Hao Lin, Junyan Chen, Shuangli Yang, Pengcheng Wang, Xinqiang Gan, Yali Wang, Bin Xu, Junjie Sun, Congcong Yin, Zongxiong Huang, Yimin Fang
Summary: The study successfully detected low-concentration exosomes in complex matrices by enriching exosomes with a filter membrane and using CuS nanoparticles as labels. The high sensitivity, specificity, and simplicity of the method make it suitable for rapid diagnosis of diseases like prostate cancer.
ANALYTICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Samridhi Banskota, David C. Adamson
Summary: Pituitary adenomas are tumors that arise in the anterior pituitary gland, most commonly benign and exerting their effects through excess hormone secretion or mass effect. The clinical presentation varies based on the size and hormone secretion of the adenoma.
Article
Cell Biology
Zhaowei Zhang, Xuehui Liu, Xiaoqing Yang, Ying Jiang, Ang Li, Jiying Cong, Yuwei Li, Qinjian Xie, Chen Xu, Dingbin Liu
Summary: In this study, it was discovered that the extracellular vesicles (EVs) in the faeces of colorectal cancer (CRC) patients can serve as a potential biomarker for the non-invasive diagnosis and prognosis of CRC. The levels of CD147 and A33 on faeces-derived EVs (fEVs) were found to be upregulated in CRC patients, distinguishing them from healthy donors. The CD147/A33-enriched fEVs offer a higher clinical sensitivity (89%) compared to the established serum biomarker CEA (40%) for CRC diagnosis.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Endocrinology & Metabolism
Kunzhe Lin, Jianping Zhang, Yinghong Lin, Zhijie Pei, Shousen Wang
Summary: This study investigated the metabolic differences between invasive and non-invasive NFPAs and found distinct metabolite profiles. It also confirmed the high expression of the M2 macrophage marker in invasive NFPAs.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Oncology
Xiao Yue, Fengming Lan, Weiping Liu
Summary: circDDX17 is downregulated in PA tissues and is associated with invasion, tumor size, and progression-free survival. Overexpression of circDDX17 significantly inhibits cell migration and invasion through miR-1279. CADM2 is a direct target of miR-1279 and silencing CADM2 reverses the tumor-suppressing effects of circDDX17. Our study demonstrates that circDDX17 upregulates CADM2 expression by sponging miR-1279, suppressing the invasive behaviors of PA.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemical Research Methods
Wenwen Chen, Rongkai Cao, Wentao Su, Xu Zhang, Yuhai Xu, Peng Wang, Zhongqiao Gan, Yingying Xie, Hongjing Li, Jianhua Qin
Summary: The study proposes a simple, quick, and label-free method for isolating circulating exosomes from patient serum. Further validation through miRNA sequencing and RT-PCR reveals a potential new biomarker.
Review
Chemistry, Physical
Yuxiang Xue, Xue Feng, Xianfeng Fan, Guangyu Zhu, James McLaughlan, Wenjun Zhang, Xianfeng Chen
Summary: This review summarizes the strategies of using EVs as biomarkers for cancer detection, evaluates their advantages and challenges, and discusses their prospects for cancer early diagnosis.
Article
Genetics & Heredity
Jiali Xiong, Haojie Zhang, Bin Zeng, Jie Liu, Junyi Luo, Ting Chen, Jiajie Sun, Qianyun Xi, Yongliang Zhang
Summary: Extracellular vesicles from the anterior pituitary of Duroc swine were separated and identified in this study, with RNA-seq revealing a large number of miRNAs, lncRNAs, and circRNAs. Analysis suggested that these ncRNAs may be involved in intracellular signal transduction, cellular component organization, and other activities. The findings provide a fundamental resource for understanding the detailed functions of extracellular vesicles from the anterior pituitary.
FRONTIERS IN GENETICS
(2021)
Article
Clinical Neurology
Kelin Chen, Guoge Li, Xixiong Kang, Pinan Liu, Lingye Qian, Yijun Shi, Rasha Alsamani Osman, Zhijun Yang, Guojun Zhang
Summary: This study aimed to investigate noninvasive methods to discover viable biomarkers for assessing the invasiveness of pituitary adenomas. Through analyzing E-cadherin, N-cadherin, Epcam, TGF-beta, Smad3, and Smad7 in tissues and exosomes, the results suggested that EMT-related biomarkers in serum exosomes could potentially be used for assessing the invasiveness of pituitary adenomas.
NEUROPSYCHIATRIC DISEASE AND TREATMENT
(2021)
Review
Biochemistry & Molecular Biology
Jianjing Lin, Li Wang, Jianhao Lin, Qiang Liu
Summary: This article discusses the role of extracellular vesicles in osteoarthritis, as well as the importance of cell pretreatment strategies and EV tissue engineering in OA treatment.
Review
Medicine, General & Internal
Na Wang, Shuai Yuan, Cheng Fang, Xiao Hu, Yu-Sen Zhang, Ling-Ling Zhang, Xian-Tao Zeng
Summary: Extracellular vesicles (EVs) are natural nanoparticles secreted by cells in the body and can be used as important markers for disease diagnosis and prognosis. Nanomaterial-based platforms have been innovatively explored for the isolation and detection of EVs from urine, providing higher selectivity and sensitivity. This review focuses on the nanomaterials used in the isolation and detection of urinary EVs, and presents urinary EV-derived biomarkers for prostate cancer diagnosis.
FRONTIERS IN MEDICINE
(2022)
Article
Biochemical Research Methods
Lihua Ding, Xia Liu, Zhenzhong Zhang, Li-e Liu, Sitian He, Yan Wu, Clement Yaw Effah, Ruiying Yang, Aiai Zhang, Wensi Chen, Mubarak Yarmamat, Lingbo Qu, Xiaonan Yang, Yongjun Wu
Summary: In this study, a microfluidic chip was designed to isolate exosomes from plasma, improving their capture efficiency and purity. The chip was also used for plasma-based cancer diagnosis by monitoring exosomal biomarkers.