Article
Agriculture, Dairy & Animal Science
Hannah J. Stage, Susanne Trappe, Katharina Soellig, Dagmar S. Trachsel, Katharina Kirsch, Cornelia Zieger, Roswitha Merle, Joerg R. Aschenbach, Heidrun Gehlen
Summary: This study investigated the proliferation and multilineage differentiation potential of equine adipose stem cells (ASCs) obtained from different tissue sources. The study found that ASCs had high proliferation and differentiation potential, but were unable to undergo cardiomyogenic differentiation. This study provides a basis for future research in equine medicine.
Article
Agriculture, Dairy & Animal Science
Ga Yeon Kim, Gyu Tae Choi, Jinryong Park, Jeongeun Lee, Jeong Tae Do
Summary: In this study, two types of porcine MSCs were compared, and it was found that adipose-derived stem cells showed better proliferation capacity, adipogenic and chondrogenic differentiation potential, and mitochondrial metabolism compared to Wharton's jelly-derived mesenchymal stem cells. This study provides insights into the characteristics of porcine MSCs and their potential applications in animal clinics and cultured meat science.
Article
Biochemistry & Molecular Biology
Ahmad Abd-El-Aziz, Angela Riveroll, Blanca Esparza-Gonsalez, Laurie McDuffee, Alejandro M. Cohen, Adam L. Fenech, William J. Montelpare
Summary: This research investigated the impact of heat stress on cell differentiation in an equine mesenchymal stem cell model. The results showed that heat stress led to specific responses in different cell types and temperatures, with differentially expressed proteins involved in cell-signaling pathways. Moreover, cytoskeletal proteins exhibited altered levels and the cells did not organize properly, suggesting long-term impacts on cellular development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mohammed Zayed, Steve Adair, Madhu Dhar
Summary: The study showed that synovial fluid can maintain the proliferation and viability of MSCs and significantly affect chondrocyte differentiation. Stimulation of MSCs with synovial fluid or IFN-gamma can enhance chondrogenesis and anti-inflammatory activity. This suggests that the joint environment is suitable for chondrogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Eun-Ju Lee, Khurshid Ahmad, Shiva Pathak, SunJu Lee, Mohammad Hassan Baig, Jee-Heon Jeong, Kyung-Oh Doh, Dong-Mok Lee, Inho Choi
Summary: This study introduces novel peptides derived from fibronectin that enhance cell adhesion, proliferation, and differentiation, offering a new approach to cell culture. The peptides showed significant effects on primary cells and stem cells, increasing proliferation and promoting differentiation into specific cell types such as osteoblasts. The results suggest potential applications in the production of core materials for cell culture such as scaffolds and culture medium components.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Erika N. Guerrero, Shantal Vega, Cindy Fu, Ruth De Leon, Davis Beltran, Mairim Alexandra Solis
Summary: Maternal age influences the biological characteristics and stemness properties of PDMSCs, with cells from mothers aged 22-35 showing higher pluripotency and differentiation effectiveness, while donors aged 26-30 exhibit greater self-renewal ability.
Article
Agriculture, Dairy & Animal Science
Manuela Heimann, Mohamed I. Elashry, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: The investigation of mesenchymal stem cells has become an interesting scientific field in regenerative medicine and organ transplantation. This study focused on establishing an adipogenic differentiation protocol suitable for different mammalian species, and found that using rosiglitazone instead of traditional protocols significantly improves cell viability and adipogenic differentiation capacity. The study also revealed differences between species in terms of fat formation and fat-regulating gene expression. This information is essential for selecting a stem cell specification program and improving clinical targeting of obesity and metabolic disorders in veterinary medicine.
Article
Biotechnology & Applied Microbiology
Hyoungki Kim, Soonjo Kwon
Summary: The study demonstrates that a hypoxic environment, created using specific chemicals in a suspension culture, enhances the proliferation of mouse clonal mesenchymal stem cells and promotes their differentiation into osteogenic cells.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Cell Biology
Gi Cheol Park, Ji Min Kim, Sung-Chan Shin, Yong-il Cheon, Eui-Suk Sung, Minhyung Lee, Jin-Choon Lee, Byung-Joo Lee
Summary: Human tonsil-derived mesenchymal stem cells (TMSCs) have a higher proliferation rate and differentiation potential compared to adipose-tissue-derived MSCs (AMSCs) or bone-marrow-derived MSCs (BMSCs). TMSCs exhibit a significantly higher expression of the tensin3 gene (TNS3) than AMSCs or BMSCs. TNS3 plays a vital role in regulating cell migration, gene and protein expression, and integrin activity in TMSCs.
Article
Biology
Rafal B. Lewandowski, Malgorzata Stepinska, Andrzej Gietka, Monika Dobrzynska, Mariusz P. Lapinski, Elzbieta A. Trafny
Summary: LED irradiation at a wavelength of 630 nm, energy density 4 J/cm(2), and power density 17 mW/cm(2) can effectively increase the number of viable hBM-MSCs in vitro while maintaining their crucial surface antigens and differentiation potential.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2021)
Review
Engineering, Biomedical
Jay R. K. Samal, Vignesh K. Rangasami, Sumanta Samanta, Oommen P. Varghese, Oommen P. Oommen
Summary: Studies have shown that culturing MSCs in a low oxygen environment can upregulate stem cell markers and promote cell proliferation in an undifferentiated state. However, there is ambiguity in the results due to differences in oxygen gradients and culture conditions among different studies. This progress report focuses on conflicting findings related to the application of hypoxic conditions for improving MSC proliferation or differentiation, aiming to decipher the factors influencing MSC characteristics under hypoxia.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Robert Soukup, Iris Gerner, Sinan Gueltekin, Hayeon Baik, Johannes Oesterreicher, Johannes Grillari, Florien Jenner
Summary: Extracellular vesicles (EVs) play an essential role in cellular crosstalk, and equine MSC-derived EVs have comparable therapeutic potential to their donor cells. The International Society for Extracellular Vesicles (ISEV) has proposed standards to define EVs and their functions, but their application in veterinary field is restricted by nonstandardised isolation methods and limited availability of cross-reacting markers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Ejaz R. Dar, Mudasir B. Gugjoo, Moien Javaid, Shahid Hussain, Mujeeb R. Fazili, Kuldeep Dhama, Taha Alqahtani, Ali M. Alqahtani, Riaz A. Shah, Talha Bin Emran
Summary: The study evaluates the influence of ambient temperature, donor physiological status, and tissue source on sheep mesenchymal stem cells. Results show that adipose tissue-derived MSCs from full term pregnant female sheep exhibit higher potential for proliferation and differentiation compared to male sheep-derived MSCs. Cryopreserved MSCs have similar characteristics to fresh cells. Therefore, it is important to consider tissue type and donor physiological status when applying MSCs in clinical settings.
Article
Neurosciences
Nader Asadian, Majid Jadidi, Manouchehr Safari, Taha Jadidi, Mahbobeh Gholami
Summary: The study revealed that sinusoidal 25 Hz, square 50 Hz, and sinusoidal 75 Hz EMF groups had negative effects on the viability and proliferation rate of bone marrow mesenchymal stem cells (BMMSC), while sinusoidal 50 Hz and 75 Hz groups promoted differentiation into neurons. Different frequencies of EMF had varying impacts on the differentiation into astrocyte cells. Further research on the effects of EMF with broader flux density and frequency on BMMSC is recommended.
NEUROSCIENCE LETTERS
(2021)
Article
Cell & Tissue Engineering
Guanyin Chen, Dong Fan, Wangqian Zhang, Shuning Wang, Jintao Gu, Yuan Gao, Lei He, Weina Li, Cun Zhang, Meng Li, Yingqi Zhang, Zhaohui Liu, Qiang Hao
Summary: This study found that under hypoxic conditions, there was a significant increase in the expression of Mkx in MSCs. Mkx mediated hypoxia-induced tenogenic differentiation of MSCs but could not completely repress the proliferation of hypoxic MSCs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Agriculture, Dairy & Animal Science
E. Martignani, S. Miretti, L. Vincenti, M. Baratta
DOMESTIC ANIMAL ENDOCRINOLOGY
(2019)
Article
Agriculture, Dairy & Animal Science
Gerardo Caja, Andreia Castro-Costa, Ahmed A. K. Salama, Joan Oliver, Mario Baratta, Carles Ferrer, Christopher H. Knight
JOURNAL OF DAIRY RESEARCH
(2020)
Article
Veterinary Sciences
C. Gola, S. Iussich, S. Noury, M. Martano, F. Gattino, E. Morello, E. Martignani, L. Maniscalco, P. Accornero, P. Buracco, L. Aresu, R. De Maria
VETERINARY JOURNAL
(2020)
Article
Multidisciplinary Sciences
E. Martignani, U. Ala, P. A. Sheehy, P. C. Thomson, M. Baratta
SCIENTIFIC REPORTS
(2020)
Article
Veterinary Sciences
Andreas Gess, Irene Viola, Silvia Miretti, Elisabetta Macchi, Giovanni Perona, Luca Battaglini, Mario Baratta
FRONTIERS IN VETERINARY SCIENCE
(2020)
Article
Cell Biology
Alessia Morato, Eugenio Martignani, Silvia Miretti, Mario Baratta, Paolo Accornero
Summary: During puberty, the mammary gland undergoes intense growth dependent on EGF receptors. Our study showed that EGFR signaling can sustain proliferation and survival of mammary epithelial cells in vitro, and identified three EGFR-ligands likely mediating this autocrine signaling.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Ursula Gonzales-Barron, Sara Coelho-Fernandes, Gisela Santos-Rodrigues, Altino Choupina, Roberto Bermudez Piedra, Koldo Osoro, Rafael Celaya, Rocio R. Garcia, Tanja Peric, Silvia Del Bianco, Edi Piasentier, Francesco Chiesa, Alberto Brugiapaglia, Luca Battaglini, Mario Baratta, Raul Bodas, Jose M. Lorenzo, Vasco A. P. Cadavez
Summary: This study investigated the spoilage microorganisms in refrigerated vacuum-packed lamb meat from 10 farms housing 8 local breeds raised in different regimes. It found that intrinsic properties of meat, such as pH, moisture, and protein content, can affect the microbial spoilage of lamb meat. The study suggests that improving animal handling, slaughtering practices, and carcass classification system could enhance the microbial quality of lamb meat.
SMALL RUMINANT RESEARCH
(2021)
Review
Veterinary Sciences
Silvia Miretti, Cristina Lecchi, Fabrizio Ceciliani, Mario Baratta
FRONTIERS IN VETERINARY SCIENCE
(2020)
Editorial Material
Veterinary Sciences
Mario Baratta, Gianfranco Gabai, Pietro Celi
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Rocio Rosa Garcia, Tanja Peric, Vasco Cadavez, Andreas Gess, Joaquim Orlando Lima Cerqueira, Ursula Gonzales-Barron, Mario Baratta
Summary: This study evaluated foliage arthropods, vegetation cover, flower numbers, and plant species richness in sheep grazed lands from 5 EU countries, finding that more extensively managed farms had higher total arthropod abundance, sward height, and plant species richness. The composition of the arthropod and pollinator communities varied between management strategies and vegetation types, with more diverse groups linked to areas under extensive management.
SMALL RUMINANT RESEARCH
(2021)
Article
Veterinary Sciences
Magdalena Arevalo-Turrubiarte, Mario Baratta, Giovanna Ponti, Elisabetta Chiaradia, Eugenio Martignani
Summary: The study found that EVs derived from equine MSCs can reduce the gene expression of metalloproteinase-13, potentially serving as an adjuvant treatment to improve tissue and cartilage repair in articular pathologies.
EQUINE VETERINARY JOURNAL
(2022)
Article
Chemistry, Analytical
Mike O. Ojo, Irene Viola, Mario Baratta, Stefano Giordano
Summary: This article introduces a low-power livestock location monitoring system based on IoT technology, which is crucial for improving animal welfare and protecting native breeds and meats in vast geographical areas suitable for livestock grazing.
Article
Agriculture, Dairy & Animal Science
Raul Bodas, Juan Jose Garcia-Garcia, Monica Montanes, Alberto Benito, Tanja Peric, Mario Baratta, Irene Viola, Andreas Gess, Nathanael Ko, Vasco Cadavez, Ursula Gonzales-Barron, Esperanza Dominguez, Sara Olmedo
Summary: The welfare of lambs reared up to 4 months of age in different management systems in Europe varies significantly. While most farms showed high compliance with the animals' needs, the subjective assessment of emotional state yielded highly variable results. Lambs raised in intensive and semi-intensive farms appeared to exhibit a different and apparently calmer mental state compared to those reared in very extensive conditions.
SMALL RUMINANT RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Isabella Manenti, Irene Viola, Ugo Ala, Paolo Cornale, Elisabetta Macchi, Paola Toschi, Eugenio Martignani, Mario Baratta, Silvia Miretti
Summary: Farm procedures have an impact on animal welfare and induce physiological responses. This study identified miRNAs expressed in sheep saliva and found significant differences in their expression patterns between cortisol clusters. The trend analysis suggests a possible cooperation between miR-16b and miR-21 in stress response.
Review
Education & Educational Research
Edlira Muca, Damiano Cavallini, Rosangela Odore, Mario Baratta, Domenico Bergero, Emanuela Valle
Summary: This study provides an overall analysis of the use of learning resources and media devices in veterinary settings. The results indicate a high use of traditional textbooks, moderate use of online tools, and low use of research papers among veterinary students. These findings suggest that educators should consider students' attitudes towards digital resources in the context of technological advancements and pandemic-related restrictions.
EDUCATION SCIENCES
(2022)