Review
Biochemistry & Molecular Biology
Giuliana Minani Bertolino, Marie Maumus, Christian Jorgensen, Daniele Noel
Summary: Extracellular vesicles (EVs) isolated from induced pluripotent stem cells (iMSCs) show promise in regenerative medicine for the treatment of various diseases. However, the heterogeneity between MSC donors may lead to batch-to-batch variability in the use of EVs as cell-free therapeutic agents.
Review
Pharmacology & Pharmacy
Lu Zhang, Xiao-Jing-Nan Ma, Yuan-Yuan Fei, Heng-Tong Han, Jun Xu, Lu Cheng, Xun Li
Summary: The liver has the ability to repair itself, but pathological changes can impair its regenerative capacity and lead to liver failure. Mesenchymal stem cells (MSCs) have shown promise in liver regeneration, and induced pluripotent stem cells have made progress in organoid establishment and drug screening.
PHARMACOLOGY & THERAPEUTICS
(2022)
Review
Biochemistry & Molecular Biology
Riccardo Ossanna, Sheila Veronese, Lindsey Alejandra Quintero Sierra, Anita Conti, Giamaica Conti, Andrea Sbarbati
Summary: Cell-based therapy has the potential to restore lost cells and promote regeneration in various human disorders. Stem cells, particularly pluripotent stem cells and recently discovered Muse cells, hold great promise for clinical medicine. Muse cells are pluripotent cells found in adult human mesenchymal tissues, offering advantages such as autologous treatment and avoidance of rejection issues in transplants. They have shown promising results in preclinical studies and could expand therapeutic applications in regenerative medicine.
Article
Chemistry, Medicinal
Aleksandra Skubis-Sikora, Bartosz Sikora, Weronika Malysiak, Patrycja Wieczorek, Piotr Czekaj
Summary: Melatonin has been evaluated for its safety and biological activity on ADSCs in vitro, and it was found that low-dose melatonin can improve cell viability without negatively affecting osteogenic potential.
Review
Biochemistry & Molecular Biology
Arsinoe C. Thomaidou, Maria Goulielmaki, Antonis Tsintarakis, Panagiotis Zoumpourlis, Marialena Toya, Ioannis Christodoulou, Vassilis Zoumpourlis
Summary: The human body contains multipotent cells known as mesenchymal stem cells (MSCs) that have the potential to self-renew and differentiate into various cell types. MSCs are attractive candidates for therapeutic applications due to their ability to migrate to inflammation sites and their secretion of factors involved in tissue regeneration and immune regulation. MSCs derived from fetal, perinatal, or neonatal tissues possess additional advantages, such as increased proliferation potential, responsiveness to environmental cues, and low immunogenicity. MicroRNAs (miRNAs) play a crucial role in regulating MSC differentiation, and the identification of relevant miRNAs and miRNA signatures can enhance the understanding of MSC biology and aid in the development of regenerative and therapeutic protocols. This review focuses on umbilical cord-derived MSCs (UCMSCs) and discusses their miRNA-guided differentiation and regulatory mechanisms, highlighting their potential applications in treating various diseases and injuries while minimizing adverse events.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Review
Biochemistry & Molecular Biology
Matin Chehelgerdi, Fereshteh Behdarvand Dehkordi, Mohammad Chehelgerdi, Hamidreza Kabiri, Hosein Salehian-Dehkordi, Mohammad Abdolvand, Sharareh Salmanizadeh, Mohsen Rashidi, Anoosha Niazmand, Saba Ahmadi, Sara Feizbakhshan, Saber Kabiri, Nasimeh Vatandoost, Tayebeh Ranjbarnejad
Summary: The emergence of iPSCs has revolutionized stem cell research and opened up new avenues for cancer treatment. iPSCs provide a unique platform for investigating the origin of cancer and can be used for personalized treatment and drug testing. This review article discusses the science behind iPSCs, the limitations and challenges in iPSC-based cancer therapy, ethical and social implications, and a comparative analysis with other stem cell types for cancer treatment. It also explores the applications of iPSCs in tumorigenesis and the future of iPSC research in this field.
Article
Biochemistry & Molecular Biology
Summer A. Helmi, Leili Rohani, Ahmed R. Zaher, Youssry M. El Hawary, Derrick E. Rancourt
Summary: Bone healing is a well-organized process regulated by multiple factors. The Notch signaling pathway plays a significant role in controlling bone mesenchymal stem cell differentiation. Inhibiting the Notch pathway can greatly enhance the differentiation of both embryonic and induced pluripotent stem cells, potentially improving overall healing outcomes. Stem cell transplantations are prominent candidates for treating critical-sized bone defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Soudeh Ghafouri-Fard, Vahid Niazi, Bashdar Mahmud Hussen, Mir Davood Omrani, Mohammad Taheri, Abbas Basiri
Summary: Extracellular vesicles play important roles in cell-cell communication, inflammation modulation, cell proliferation, and tissue regeneration, with advantages such as lower risk and easier production and storage compared to cell therapy. Particularly, exosomes derived from mesenchymal stem cells are introduced as a therapeutic option for various human diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Tzu-Cheng Sung, Yi-Peng Jiang, Jhe-Yu Hsu, Qing-Dong Ling, Hao Chen, Suresh S. Kumar, Yung Chang, Shih-Tien Hsu, Qingsong Ye, Akon Higuchi
Summary: This study successfully generated universal hiPSCs without gene editing and evaluated their resistance to immune reactions, demonstrating that the cells maintained stable characteristics even after continuous passages.
CELL PROLIFERATION
(2021)
Article
Agriculture, Dairy & Animal Science
Michal Domaniza, Marian Hluchy, Dasa Cizkova, Filip Humenik, Lucia Slovinska, Nikola Hudakova, Lubica Hornakova, Juraj Vozar, Alexandra Trbolova
Summary: This study aimed to investigate the potential of cell-based regenerative therapy for elbow joints affected by osteoarthritis. The results showed that two injections of amnion-derived mesenchymal stem cells improved joint range of motion and had an impact on synovial fluid biomarkers. Concentrations of IFN-γ, IL-6, and TNF-α decreased while IL-10 increased between day 0 and day 28 after treatment.
Article
Biochemistry & Molecular Biology
Mohammad Mousaei Ghasroldasht, Jin Seok, Hang-Soo Park, Farzana Begum Liakath Ali, Ayman Al-Hendy
Summary: Regenerative medicine is a promising therapeutic mode using stem cells for the treatment of challenging human diseases. Stem cells have pleotropic therapeutic potential through inflammation or apoptosis inhibition, cell recruitment, angiogenesis stimulation, and differentiation. This review provides an overview of the translation process from idea to clinical practice, including guidelines for clinical trial approval and a review of regenerative medicine clinical trials registered on the Clinicaltrials.gov website.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Diogo E. S. Nogueira, Joaquim M. S. Cabral, Carlos A. V. Rodrigues
Summary: Research has shown the potential of human stem cells in regenerative medicine, but their use in patients requires large-scale expansion systems. Single-use bioreactors are recommended to reduce the risk of product contamination in stem cell biomanufacturing at a large scale.
BIOENGINEERING-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Ruei-Hong Lin, Hsu-Tung Lee, Chun-An Yeh, Yi-Chin Yang, Chiung-Chyi Shen, Kai-Bo Chang, Bai-Shuan Liu, Hsien-Hsu Hsieh, Hui-Min David Wang, Huey-Shan Hung
Summary: The research shows that using AuNPs at concentrations of 1.25 and 2.5 ppm can enhance cell proliferation, reduce reactive oxygen species generation, and attenuate platelet/monocyte activation. AuNPs can also promote cell adhesion, increase the expression of matrix metalloproteinase (MMP-2/9), decrease cell apoptosis and the release of inflammatory cytokines. Animal model experiments demonstrate that treating with 1.25 and 2.5 ppm AuNPs exhibits better biocompatibility and endothelialization. This suggests that AuNPs can be used as a biocompatible nanomedicine for regeneration research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Riccardo Giorgino, Domenico Albano, Stefano Fusco, Giuseppe M. Peretti, Laura Mangiavini, Carmelo Messina
Summary: Osteoarthritis (OA) is a common chronic orthopedic disorder that primarily affects hips and knees. The variability in the prevalence of knee OA across studies highlights the heterogeneity of reported data worldwide. The pathogenetic mechanism of knee OA involves an inflammatory process in the synovial membrane, mediated by immune system activation and damage-associated molecular patterns (DAMPs). Mesenchymal stem cells (MSCs) offer promising therapeutic options, but unresolved issues remain regarding the optimal cell source and quantity. Further research is necessary to address these uncertainties. This review provides a comprehensive overview of the molecular mechanisms of cartilage damage in knee OA and discusses current regenerative medicine approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Karolina Pircs, Janelle Drouin-Ouellet, Vivien Horvath, Jeovanis Gil, Melinda Rezeli, Raquel Garza, Daniela A. Grassi, Yogita Sharma, Isabelle St-Amour, Kate Harris, Marie E. Jonsson, Pia A. Johansson, Romina Vuono, Shaline Fazal, Thomas Stoker, Bob A. Hersbach, Kritika Sharma, Jessica Lagerwall, Stina Lagerstrom, Petter Storm, Sebastien S. Hebert, Gyorgy Marko-Varga, Malin Parmar, Roger A. Barker, Johan Jakobsson
Summary: Reprogramming elderly patients' skin fibroblasts into neurons revealed specific autophagy deficits in Huntington's disease induced neurons, highlighting the importance of wild-type HTT in normal neuronal autophagy and providing rationale for future autophagy activation therapies.
Article
Cell & Tissue Engineering
Pia A. Johansson, Per Ludvik Brattas, Christopher H. Douse, PingHsun Hsieh, Anita Adami, Julien Pontis, Daniela Grassi, Raquel Garza, Edoardo Sozzi, Rodrigo Cataldo, Marie E. Jonsson, Diahann A. M. Atacho, Karolina Pircs, Feride Eren, Yogita Sharma, Jenny Johansson, Alessandro Fiorenzano, Malin Parmar, Malin Fex, Didier Trono, Evan E. Eichler, Johan Jakobsson
Summary: Despite limited changes in protein-coding genes, the human forebrain has expanded in size and complexity compared to chimpanzees. This suggests that gene expression regulation, rather than genetic changes, plays a significant role in brain evolution. Researchers have identified a transcription factor called ZNF558 that is expressed in human forebrain neural progenitor cells but not in chimpanzees. ZNF558 functions in mitochondrial homeostasis and influences developmental timing during early human brain development. Its expression is controlled by a variable number tandem repeat, which is longer in chimpanzees and shows variability within the human population. This study provides mechanistic insights into how regulatory networks are established through structural variations, affecting human brain evolution.
Article
Cell & Tissue Engineering
Patrick Aldrin-Kirk, Malin Akerblom, Tiago Cardoso, Sara Nolbrant, Andrew F. Adler, Xiaohe Liu, Andreas Heuer, Marcus Davidsson, Malin Parmar, Tomas Bjorklund
Summary: Transplanting hESC-derived DA neurons in Parkinson's disease using a novel virus vector to observe their projections, revealing potential integration with host circuitry based on differential afferent inputs.
Article
Multidisciplinary Sciences
Zhanna Alekseenko, Jose M. Dias, Andrew F. Adler, Mariya Kozhevnikova, Josina Anna van Lunteren, Sara Nolbrant, Ashwini Jeggari, Svitlana Vasylovska, Takashi Yoshitake, Jan Kehr, Marie Carlen, Andrey Alexeyenko, Malin Parmar, Johan Ericson
Summary: Stem cell therapies utilizing retinoic acid (RA) signaling have shown promise in providing treatment for Parkinson's disease, by deriving dopamine neurons and restoring motor deficits in animal models.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Yunsong Jiang, Tugce Torun, Sara M. Maffioletti, Andrea Serio, Francesco Saverio Tedesco
Summary: Engineering models of human skeletal muscle tissue provide unique opportunities for studying acute muscle injuries, neuro-muscular disorders, and musculo-skeletal disorders in vitro. These models involve isolating, generating, amplifying, and combining various cell types with scaffolds and biomaterials. Novel bio-fabrication strategies and emerging technologies, such as 3D bioprinting and organoids, offer potential solutions to technical challenges and further enhance tissue functionalization.
EXPERIMENTAL CELL RESEARCH
(2022)
Editorial Material
Immunology
Valentin P. Shichkin, Maria Pia Felli, Isabella Screpanti, Mariastefania Antica
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Valentin P. Shichkin, Mariastefania Antica
Summary: The thymus is a complex organ consisting of different stromal cells and maturing T lymphocytes, and its function is mainly determined by the thymic stromal structure. Thymic epithelial cells and mesenchymal cells are the primary components of the thymus, playing a crucial role in T cell development and function. Understanding the signaling and transcriptional pathways underlying thymic cell interaction is essential for T cell differentiation and restoring thymic function after damage.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
SungWoo Choi, Giulia Ferrari, Louise A. Moyle, Kirsty Mackinlay, Naira Naouar, Salma Jalal, Sara Benedetti, Christine Wells, Francesco Muntoni, Francesco Saverio Tedesco
Summary: Muscle satellite stem cells (MuSCs) play a crucial role in muscle growth and regeneration. Researchers have found that DLL4 and PDGF-BB can enhance the migration capacity of myogenic progenitors derived from human induced pluripotent stem cells (hiPSCs), bringing hiPSCs closer to potential applications in muscle gene and cell therapies.
EMBO MOLECULAR MEDICINE
(2022)
Article
Cell Biology
Edoardo Sozzi, Janko Kajtez, Andreas Bruzelius, Milan Finn Wesseler, Fredrik Nilsson, Marcella Birtele, Niels B. Larsen, Daniella Rylander Ottosson, Petter Storm, Malin Parmar, Alessandro Fiorenzano
Summary: Human pluripotent stem cells can self-organize into cerebral organoids resembling developing human brain tissue. However, current methods often lead to intra-organoid variability and incomplete maturation. In this study, a silk microfiber network scaffold was used to drive stem cells to arrange into engineered brain organoids, resulting in more homogeneous and functionally mature neuronal properties.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Jana Rajova, Marcus Davidsson, Martino Avallone, Morgan Hartnor, Patrick Aldrin-Kirk, Tiago Cardoso, Annelie Mollbrink, Sara Nolbrant, Petter Storm, Andreas Heuer, Malin Parmar, Tomas Bjorklund
Summary: Cell therapy for Parkinson's disease has shown substantial growth, but the transcriptomic analysis of fully differentiated grafts has not been thoroughly investigated. Through spatial transcriptomics analysis, it was found that cells derived from human embryonic stem cells adopt mature dopaminergic signatures. These findings support the preferred environmental niche of dopamine neurons and confirm their dopaminergic phenotype.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Biochemical Research Methods
Jessica Giacomoni, Mette Habekost, Efrain Cepeda-Prado, Bengt Mattsson, Daniella Rylander Ottosson, Malin Parmar, Janko Kajtez
Summary: In this study, a simple protocol is presented for slicing, immunostaining, and clearing sections of adult rat brains for high-resolution confocal imaging. The protocol does not require toxic reagents or specialized equipment. Instructions for culturing rat brain slices floating on permeable culture inserts, maintained in regular CO2 incubators, and handled only at media change are also provided.
Article
Biochemical Research Methods
Fatemeh Safi, Parashar Dhapola, Eva Erlandsson, Linda Geironson Ulfsson, Ariana S. Caldero, Charlotta Boiers, Goran Karlsson
Summary: In this study, an in vitro co-culture system was developed that can differentiate hematopoietic progenitor populations to all major hematopoietic lineages at the clonal level. Two different protocols, a single-cell switch-culture system and a barcoding protocol, were presented for cell differentiation. The generation of all lineages from single long-term hematopoietic stem cells was achieved after 21 days of culture, making this protocol a valuable tool for single-cell genomics validation experiments.
Article
Cell & Tissue Engineering
Agnete Kirkeby, Jenny Nelander, Deirdre B. Hoban, Nina Rogelius, Hjalmar Bjartmarz, Petter Storm, Alessandro Fiorenzano, Andrew F. Adler, Shelby Vale, Janitha Mudannayake, Yu Zhang, Tiago Cardoso, Bengt Mattsson, Anne M. Landau, Andreas N. Glud, Jens C. Sorensen, Thea P. Lillethorup, Mark Lowdell, Carla Carvalho, Owen Bain, Trinette van Vliet, Olle Lindvall, Anders Bjorklund, Bronwen Harry, Emma Cutting, Hakan Widner, Gesine Paul, Roger A. Barker, Malin Parmar
Summary: This paper presents the quality, safety, and efficacy data supporting a clinical trial for cell replacement therapies in Parkinson's disease. The transplantation of dopaminergic neurons derived from pluripotent stem cells has shown positive results in pre-clinical studies. The first-in-human clinical trial has been initiated with promising outcomes.
Article
Cell & Tissue Engineering
Roberta Schellino, Dario Besusso, Roberta Parolisi, Gabriela B. B. Gomez-Gonzalez, Sveva Dallere, Linda Scaramuzza, Marta Ribodino, Ilaria Campus, Paola Conforti, Malin Parmar, Marina Boido, Elena Cattaneo, Annalisa Buffo
Summary: This study investigated the therapeutic potential of hESC-derived striatal progenitor grafts for Huntington's disease. The results showed that the grafts survived and improved motor deficits in the HD model rats. Environmental enrichment also accelerated cell maturation and promoted integration with host cells.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Immunology
Valentin P. Shichkin, Oleg V. Kurchenko, Elena N. Okhotnikova, Valentyna V. Chopyak, Domenico V. Delfino
Summary: This review analyzes the mechanisms and factors associated with IgE-associated allergic diseases caused by food allergens and discusses the clinical symptoms and current treatment approaches for food allergies. The review also emphasizes the use of enterosorbents in the complex therapy of food allergies and related manifestations, such as skin and respiratory symptoms associated with gastroenterological disorders. It summarizes the experience of using enterosorbents for detoxification purposes in post-Soviet countries, with a special focus on the enterosorbent White Coal (Carbowhite) based on silicon dioxide produced by the Ukrainian company OmniFarma.
FRONTIERS IN IMMUNOLOGY
(2023)
