Article
Chemistry, Analytical
Qingling Li, Yuehan Xu, Ji Qi, Xiaoli Zheng, Shudi Liu, Dong Lin, Liangwei Zhang, Ping Liu, Bowei Li, Lingxin Chen
Summary: This article introduces a self-powered rotating paper-based microfluidic chip for detecting thrombin. The chip has the ability to control fluid and perform complex multistep operations. The experimental results show that the chip has a wide linear range and a low detection limit, with potential applications in on-site diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemical Research Methods
Baruch Rofman, Rawi Naddaf, Maya Bar-Dolev, Tal Gefen, Nadav Ben-Assa, Naama Geva-Zatorsky, Moran Bercovici
Summary: We have developed a controllable valve that combines electroosmotic-flow generation and hydrophobic air gap in porous media, allowing for multi-step assays on paper-based devices. Our model describes the flow and control parameters, and we have successfully constructed a stable and practical device. The device has been demonstrated for amplification of SARS-CoV-2 sequences directly from raw saliva samples.
Article
Biochemical Research Methods
Dohwan Lee, Norh Asmare, A. Fatih Sarioglu
Summary: This paper reports a dipstick ELISA test that can automate the detection process and report the titer of a target molecule in a digital manner without the need for external instruments or operators. The test measures titer by gradually immune-depleting the target analyte from a flowing sample, eliminating the time-consuming pipetting steps. The technology is applied to measure SARS-CoV-2 antibody titers and troponin I, a cardiac biomarker.
Article
Multidisciplinary Sciences
Subhashis Patari, Priyankan Datta, Pallab Sinha Mahapatra
Summary: This study developed a three-dimensional microfluidic device capable of simultaneously detecting multiple chemical adulterants in milk, requiring only 1-2 mL of sample volume to detect 7 adulterants at once. The simple, portable, and user-friendly device is expected to be used for testing liquid foods in resource-limited settings.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Electrical & Electronic
Jaligam Murali Mohan, Sanjeet Kumar, Khairunnisa Amreen, Arshad Javed, Satish Kumar Dubey, Sanket Goel
Summary: One of the most common plasticizers that can cause endocrine disruption is phthalates, which are widely used in the production of consumer plastic items. This increases the risk of exposure to phthalates in items stored in water bottles, beverage drinks, and food. The detection of phthalates is crucial for food safety and quality. A point-of-care, screen-printed, disposable paper device was developed for the detection of phthalates, integrated with buckypaper for efficient detection. The device showed promising results and has the potential to be used as a point-of-care testing platform.
IEEE SENSORS JOURNAL
(2023)
Article
Biophysics
Xue Lv, Lin Zhu, Yumeng Rong, Huihui Shi, Lina Zhang, Jinghua Yu, Yan Zhang
Summary: In this study, a ratiometric electrochemiluminescence lab-on-paper platform was developed for accurate and sensitive analysis of DNA methylation, based on the enhancing effect of GCAL and the quenching effect of CQDs, as well as the high conductivity copper paper electrode. The method achieved wide linear relationship and low detection limit, providing a rapid and low-cost approach for specific determination of methylated DNA.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Lang Peng, Cunli Wang, Qiwen Lin, Yongxin Chang, Xiaoyu Zhang, Jing Wang, Zan Li, Zhiying Yang, Wenjing Sun, Wenqi Lu, Dongdong Wang, Guangyan Qing
Summary: Oxidation and protein phosphorylation are critical in regulating cellular activities. A new nanochannel device has been developed to detect both oxidation and protein phosphorylation using modified peptides. The device shows a sensitive response to reactive oxygen species and phosphorylated peptides, enabling the detection of cellular signaling pathways and kinase activity.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Pamula Sri Sruthi, Sivasamy Balasubramanian, Ponnusamy Senthil Kumar, Ashish Kapoor, Muthamilselvi Ponnuchamy, Meenu Mariam Jacob, Sivaraman Prabhakar
Summary: This study developed a miniaturized paper-based pH detection platform using natural dye extracted from red cabbage. The device's performance at different pH values was quantitatively assessed through digital image analysis, and the a* parameter in the CIEL*a*b* color space model was used for quantifying pH variations. The simplicity of fabrication, eco-friendliness due to the use of paper and natural dye, and suitability for in situ pH measurements make this methodology promising for future chemical sensor technologies.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Wan Wang, Shou-Nian Ding, Fang -Fang Chen, Qing Zhang
Summary: In this article, we described a novel paper-based device for flow injection analysis. The device utilizes capillary and a microheater to generate continuous flow in a paper strip, and it has been optimized and characterized using chronoamperometry. The device showed great potential for convenient and high-performance FIA system on paper substrates.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Takeshi Komatsu, Yuki Sato, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi
Summary: Competitive immunoassays are the standard means of detecting small molecules, but traditional methods are difficult to apply during point-of-care tests. Paper-based analytical devices are rapid and straightforward, but there are few devices proposed for competitive immunoassays.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Hui-Min Zhai, Bin Ji, Shan-Shan Tian, Fang Fang, Shuang Zhao, Zhi-Yong Wu
Summary: This study introduces a new method for Cr speciation analysis based on a paper-based analytical device (PAD), which uses electrokinetic separation and stacking to separate and recover Cr (VI) and Cr (III) with high sensitivity and recovery rates. This fast, cheap, and easy-to-use sample pretreatment method can be applied for direct analysis of Cr (VI)/Cr (III) in electroplating wastewater samples.
Article
Chemistry, Physical
Hongbing Li, Yudong Zong, Qijun Ding, Wenjia Han, Xia Li
Summary: This study developed a nanocomposite thermoelectric material using multi-walled carbon nanotube (MWCNT) and carboxylated nanocellulose fiber (C-CNF), resulting in a free-standing paper-based thermoelectric generator with excellent mechanical flexibility and reduced thermal conductivity. The TEG prototype demonstrated promising voltage and power output under a temperature gradient of 70 K, showcasing improved thermoelectric performance and a new strategy for low-cost and environmentally friendly generators.
JOURNAL OF POWER SOURCES
(2021)
Article
Chemistry, Multidisciplinary
Wei Xue, Dan Zhao, Qiang Zhang, Yangyang Chang, Meng Liu
Summary: The study developed a paper-based origami analytical device for rapid and high-throughput assessment of multiple DNA damages induced by chemical agents.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Applied
Huimin Lu, Miaosi Li, Azadeh Nilghaz, Lizi Li, Guangxue Chen, Yanbin Jiang, Junfei Tian
Summary: A low-cost and portable paper-based analytical device has been developed for high throughput and on-site monitoring tetracycline residue in milk through visualized colorimetric reaction. The device showed good recovery rates when tested on 18 different types of real milk samples, demonstrating its potential for future application in food and environmental safety. This simple, fast, cost-effective, and equipment-free platform provides a promising tool for monitoring tetracycline in milk.
Article
Biophysics
Myunghun Kim, Youngju Kwon, Yoomin Ahn
Summary: This study developed eco-friendly and disposable paper-based membraneless microfluidic enzymatic fuel cells (EFCs) without any mediators to reduce toxicity and cost. Glucose oxidase and laccase were immobilized on multi-walled carbon nanotube electrodes to catalyze the redox reaction of glucose and oxygen, with experimental results showing that glucose concentration affects cell performance.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Nanoscience & Nanotechnology
Yaqi Liu, Fengyi Wang, Shuo Ge, Lu Zhang, Zuhao Zhang, Yunqing Liu, Yan Zhang, Shenguang Ge, Jinghua Yu
Summary: A strand displacement amplification (SDA)-assisted CRISPR/Cas12a electrochemiluminescence (ECL) biosensor was developed for ultrasensitive detection of Staphylococcus aureus (Sa)-16S rDNA. The biosensor utilized a porphyrinic Zr metal-organic framework (MOF) as a coreactant accelerator to enhance the ECL emission signal. The presence of Sa-16S rDNA triggered the SDA reaction, generating single-stranded DNA products (SP) that activated the CRISPR/Cas12a trans-cleavage and effectively extinguished the ECL signals. The ECL platform exhibited high sensitivity and specificity, making it suitable for clinical diagnosis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Qingxin Zhang, Manru Zhang, Zhiqiang Guo, Jingjing Li, Zhixue Zhu, Yu Wang, Su Liu, Jiadong Huang, Jinghua Yu
Summary: In this study, a low-background electrochemical biosensor based on a DNA tetrahedron-besieged primer and a DNAzyme-activated programmatic rolling circle amplification (RCA) was proposed for OTA detection in wine samples. The low-background detection was achieved by using the besieged primer and the target OTA-mediated DNAzyme activation initiated the programmatic RCA. The integration of the catalytic property of silver nanoclusters (AgNCs) with the electrochemical assay provided high sensitivity for OTA detection. This novel design showed great potential in food safety and environmental detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Physical
Shuai Yu, Xujie Gao, Shenguang Ge, Yuanchang Ma, Yaqi Han, Xingzhu Wang, Fumin Xue, Long Fu
Summary: The solubility of glimepiride in five binary solvent mixtures was measured using a gravimetric method. The solubility order in selected solvents was DMA + acetonitrile > DMA + methanol > DMA + ethanol > DMA + n-propanol approx. DMA + i-propanol. Solubility increased with temperature and DMA content. Four thermodynamic models were used to correlate the solubility data, with the NRTL and JAA models fitting the data the best. The solubility data in mixed solvents will be useful for the preparation and purification of this active pharmaceutical ingredient.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Analytical
Xu Li, Jiali Huang, Jiayu Ding, Mingzhen Xiu, Kang Huang, Kang Cui, Jing Zhang, Shiji Hao, Yan Zhang, Jinghua Yu, Yizhong Huang
Summary: In this study, a dual-mode lab-on-paper device was constructed using BiVO4/FeOOH nanocomposites as an efficient photoelectrochemical (PEC)/colorimetric signal reporter for sensitive detection of carcinoembryonic antigen (CEA). The nanocomposites with an FeOOH needle-like structure displayed enhanced PEC response activity, enabling quantitative detection of CEA with the PEC signal readout mode. Additionally, a colorimetric signal readout system was achieved through a smart folding technique, providing a dual-mode signal readout for the lab-on-paper device.
Article
Chemistry, Analytical
Qian Wang, Liang Lv, Wenhao Chi, Yujiao Bai, Wenqing Gao, Peihua Zhu, Jinghua Yu
Summary: A new porphyrin-based porous covalent organic framework (TAP-COF) with high surface areas and efficient electron transport was synthesized. The TAP-COF exhibited excellent peroxidase-like catalytic activity and was able to catalyze the oxidation of a substrate by hydrogen peroxide. Simple and rapid colorimetric methods for in situ hydrogen peroxide detection were developed using this TAP-COF nanozyme, which has potential applications in point-of-care testing, cell biology, and clinical diagnosis.
Article
Chemistry, Analytical
Huihui Shi, Yanfei Che, Yumeng Rong, Jiajun Wang, Yanhu Wang, Jinghua Yu, Yan Zhang
Summary: An integrated dual-signal bioassay was developed for ultrasensitive detection of thrombin. This method combined visualization with photoelectrochemical technique to achieve the detection. The bioassay demonstrated wide linear ranges, high selectivity, stability, and reproducibility under optimized conditions. Additionally, the proposed visual/photoelectrochemical transduction mechanism platform offered a lower background signal and more reliable detection results, providing an effective way for detecting other proteins and nucleic acids.
Article
Chemistry, Analytical
Wenli Xiu, Peini Zhao, Yujie Pan, Xiaoru Wang, Lina Zhang, Shenguang Ge, Jinghua Yu
Summary: A surface-enhanced Raman scattering (SERS) sensor was developed for rapid on-site determination of ethephon in food, based on the folding and assembly characteristics of paper fibers, the skeleton controllability of metal-organic framework materials (MOFs), and the morphology designability of plasmonic noble metal materials. The sensor used HKUST-1(Cu) assembled onto a carbon-treated chromatographic paper matrix to overcome poor affinity of SERS substrate for target molecules. With the coupling of biomimetic antibodies and a multi-stage progressive enhancement structure, the sensor achieved high specificity and sensitivity for ethephon detection with a linear range of 10-3 to 10 mg kg-1 and a low detection limit of around 1.39 x 10-4 mg kg-1.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Li Shan, Yuanyuan Chen, Xiaoran Tan, Shenguang Ge, Lina Zhang, Lin Li, Jinghua Yu, Li Li
Summary: A newly designed two-in-one tetrahedral DNA (TDN) nanostructure with an antifouling surface and backbone-rigidified interfacial tracks was developed for highly sensitive and selective detection of miRNA-182-5p. The TDN-modified sensing interfaces demonstrated incomparable antifouling ability against protein samples and interfering miRNAs due to the strong hydrophilic capacity and special molecular conformations. The designed TDN-modified paper-based photoelectro-chemical sensor successfully quantified miRNA-182-5p with a low detection limit of 0.09 fM and high specificity in real samples, showing great promise for bioanalysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Shanshan Li, Haihan Yu, Yuwen Wang, Shuai Wang, Lina Zhang, Peihua Zhu, Chaomin Gao, Jinghua Yu
Summary: A stable organic-inorganic Z-scheme heterostructure was constructed by covalently connecting COF-366-Fe and CeO2 through Schiff base reaction, which effectively integrated their respective properties and improved the separation and transfer of photoexcited carriers. The COF-366-Fe@CeO2 exhibited superior photocatalytic CO yield compared to COF-366-Fe and CeO2, indicating its potential as a highly-effective COF-based photocatalyst for artificial photosynthesis.
APPLIED SURFACE SCIENCE
(2023)
Article
Biophysics
Lin Li, Hui Tian, Guofu Wang, Suyue Ren, Tinglei Ma, Yixiang Wang, Shenguang Ge, Yan Zhang, Jinghua Yu
Summary: Ready-to-use in vitro diagnosis is crucial for cervical cancer prevention and treatment. A paper-film-based differential lateral flow biosensor is proposed, allowing simultaneous detection of two high-risk HPV types. The biosensor utilizes a CuCo2S4/ZnIn2S4 heterostructure on a paper-film chip and introduces surface sulfur vacancy for enhanced photoexcitation. Through sequential solution transfer, the biosensor activates the self-assembly of DNA and the cleavage activity of CRISPR-Cas12a, resulting in the release of polydopamine-coated gold nanoparticles. This intelligent biosensor achieves low limit-of-detection for HPV-18 and HPV-16 and provides a promising approach for home medical intelligent diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Inorganic & Nuclear
Wenhao Wang, Lu Zhang, Yanli Kang, Xiaodong Yang, Shenguang Ge, Feng Yu
Summary: A TiO2-CoFe2O4 heterostructure was successfully prepared using a hydrothermal method for detecting toluene gas. The heterojunction exhibited a shorter bandgap and inhibited electron-hole pair recombination, leading to improved gas sensitivity. The response value of the heterojunction was significantly higher than that of pure TiO2 and CoFe2O4, especially under UV irradiation. Density functional theory was also applied in this study to explain the improvement in gas sensitivity through material energy band and adsorption energy calculations. This work provides valuable insights for the preparation of high-efficiency and high-sensitivity gas sensors.
DALTON TRANSACTIONS
(2023)
