Article
Biotechnology & Applied Microbiology
Seo-Young Choi, Soochong Kim, Kyung-Mee Park
Summary: This study evaluated the initial healing effects of PRP gel and PRF on deep corneal wounds. The results showed that PRP gel promoted vascular healing and myofibroblast production, contributing to corneal remodeling. However, the use of PRF was not recommended due to its potential complications during tissue healing.
BIOENGINEERING-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Eduardo Anitua, Francisco Muruzabal, Ander Pino, Roberto Prado, Mikel Azkargorta, Felix Elortza, Jesus Merayo-Lloves
Summary: The study compared the effects of autologous serum (AS) and plasma rich in growth factors (PRGF) on corneal cells, revealing that undiluted AS activates pathways related to inflammation, angiogenesis, oxidative stress, and scarring response. PRGF may be a better treatment option than AS for ocular surface disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Michelangelo Palco, Paolo Rizzo, Giorgio Carmelo Basile, Angelo Alito, Daniele Bruschetta, Maria Accorinti, Roberto Restuccia, Danilo Leonetti
Summary: This study compared the effectiveness of L-PRP and PRP+HA in patients with mild to moderate HOA, finding that both treatments were effective in reducing pain, with L-PRP showing better results in reducing VAS over time, making it a potentially preferred treatment option. Further research is needed to better describe the clinical outcome of these formulations.
Article
Biochemistry & Molecular Biology
Eduardo Anitua, Ander Pino, Mikel Azkargorta, Felix Elortza, Roberto Prado
Summary: The composition differences between autologous topical serum (ES) and leukocyte- and platelet-rich plasma (L-PRP) lead to different responses in human dermal fibroblasts (HDFs). ES treatment promotes protein synthesis, cell proliferation, and migration, while L-PRP treatment induces a response similar to proinflammatory molecules.
Article
Engineering, Biomedical
Eduardo Anitua, Mar Zalduendo, Maria Troya, Roberto Tierno, Mohammad H. Alkhraisat
Summary: Scaffolds play a crucial role in tissue regeneration by providing structural support and interacting with cells and bioactive molecules. Platelet Rich Plasma (PRP) fibrin, as a successful scaffold, has significant biological effects and clinical potential. This study investigated the influence of cellular composition on fibrin membrane stability and remodeling cell activity, showing that leukocytes in PRP fibrin membranes reduce scaffold stability and induce behavioral changes in fibroblasts.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Article
Pharmacology & Pharmacy
Noelia Ruzafa, Xandra Pereiro, Alex Fonollosa, Javier Araiz, Arantxa Acera, Elena Vecino
Summary: PRGF compromises the survival of RGCs but promotes the survival of Muller cells, potentially contributing to retina healing, with further studies needed to evaluate its effect on neurons and its inflammatory role.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Orthopedics
Aazad Abbas, Jin Tong Du, Herman S. Dhotar
Summary: The leukocyte concentration of platelet-rich plasma (PRP) does not have a significant impact on patient-reported outcomes for knee osteoarthritis. According to the surface under the cumulative ranking probabilities, leukocyte-poor PRP is preferred over leukocyte-rich PRP in terms of treatment outcomes.
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2022)
Article
Biochemistry & Molecular Biology
Ronald M. Sanchez-Avila, Natalia Vazquez, Manuel Chacon, Mairobi Persinal-Medina, Agustin Brea-Pastor, Silvia Berisa-Prado, Luis Fernandez-Vega-Cueto, Eduardo Anitua, Alvaro Meana, Jesus Merayo-Lloves
Summary: This study demonstrates the positive effects of using Fibrin-Plasma Rich in Growth Factors (PRGF) membranes for the treatment of rabbit alkali-burn lesions, specifically in restoring the corneal surface. After a 40-day follow-up, only fibrin-PRGF membranes cultured with rabbit Limbal Epithelial Progenitor Cells (LEPCs) were able to achieve corneal surface restoration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Orthopedics
Xinyu Tao, Angeline Ai Ling Aw, Jun Jie Leeu, Hamid Rahmatullah Bin Abd Razak
Summary: This study compared the efficacy of a single dose of platelet-rich plasma (PRP) with multiple doses of PRP therapy in the treatment of knee osteoarthritis (KOA). The results showed that triple-dose PRP therapy provided significantly better pain relief at 12 months compared to single-dose PRP therapy, while there was no significant difference in pain relief between double-dose PRP and single-dose PRP at 12 months. In terms of safety, double-dose and triple-dose PRP therapy showed no significant differences compared to single-dose therapy.
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2023)
Article
Orthopedics
Ron Gilat, Eric D. Haunschild, Derrick M. Knapik, Aghogho Evuarherhe, Kevin C. Parvaresh, Brian J. Cole
Summary: Intra-articular injections of hyaluronic acid (HA) and platelet-rich plasma (PRP) have shown short-term improvement in pain and function for patients with symptomatic knee osteoarthritis. PRP injections, particularly with leukocyte-poor formulations, may provide greater therapeutic relief. Combination formulations of HA-PRP conjugates may also have a synergistic effect for clinically significant improvement in pain and function. Further studies on the efficacy of HA-PRP conjugates are warranted.
INTERNATIONAL ORTHOPAEDICS
(2021)
Article
Medicine, General & Internal
Antonio Rios Luna, Homid Fahandezh-Saddi Diaz, Manuel Villanueva Martinez, Angel Bueno Horcajadas, Roberto Prado, Eduardo Anitua, Sabino Padilla
Summary: This article presents a novel surgical technique for the reconstruction of chronic proximal hamstring tear, addressing the challenges posed by tendon retraction and sciatic nerve entrapment. It involves using a semitendinosus tendon allograft and injecting plasma rich in growth factors to promote healing and protect the nerve.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Materials Science, Multidisciplinary
Haiyang Yu, Guobao Wang, Jiahui Zhang, Yuhao Hong, Kunxi Zhang, Haiyan Cui
Summary: This study developed a microspheres powder that can form a gel cream when combined with platelet-rich plasma (PRP), promoting wound healing. The PRP-granular gel can be spread evenly on the wound area due to its permeability and electrostatic action. It alters the gene expression and changes the process of wound healing.
APPLIED MATERIALS TODAY
(2022)
Article
Orthopedics
Andrew G. Chan, Jeffrey D. Hoffmann, John J. Pisquiy, Philip J. Belmont, John C. Dunn
Summary: This study evaluated the quality of evidence of prospective RCTs investigating PRP treatments for knee osteoarthritis, finding that high clinical evidence does not necessarily correlate with high-quality scientific studies. Despite being rated as Level I evidence, deficiencies were identified in the Coleman Methodology Score and CONSORT checklist.
JOURNAL OF ORTHOPAEDIC SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Eduardo Anitua, Ander Pino, Mikel Azkargorta, Felix Elortza, Jesus Merayo-Lloves, Francisco Muruzabal
Summary: This study analyzed the proteomic composition of PRGF and PRGF lyo eye drops and investigated their effects on HK cells. The results showed no significant differences in the proteomic profile or the effects on HK cells between the two blood-derived formulations.
Article
Anatomy & Morphology
Eduardo Anitua, Mar Zalduendo, Maria Troya, Roberto Tierno, Mohammad H. Alkhraisat
Summary: The study evaluated the effects of leukocytes on the stability and cellular responses in PRGF scaffolds. Different cell phenotypes impacted the fibrinolysis and matrix deposition process in the clots. Overall, inclusion of leukocytes in PRGF scaffolds reduced stability, cell proliferation, and slowed down cell remodeling.
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2022)
Article
Chemistry, Medicinal
Alicia Arranz-Romera, Maria Hernandez, Patricia Checa-Casalengua, Alfredo Garcia-Layana, Irene T. Molina-Martinez, Sergio Recalde, Michael J. Young, Budd A. Tucker, Rocio Herrero-Vanrell, Patricia Fernandez-Robredo, Irene Bravo-Osuna
Summary: The study evaluated the sustained delivery effect of PLGA/VitE microspheres loaded with neurotrophic factors on the migration of retinal cells and the survival of RGCs, demonstrating a positive impact on RPE cell migration and high cell viability in vitro.
Article
Pharmacology & Pharmacy
Deyanira Barbosa-Alfaro, Vanessa Andres-Guerrero, Ivan Fernandez-Bueno, Maria Teresa Garcia-Gutierrez, Esther Gil-Alegre, Irene Teresa Molina-Martinez, Jose Carlos Pastor-Jimeno, Rocio Herrero-Vanrell, Irene Bravo-Osuna
Summary: In this study, dexamethasone-loaded PLGA microspheres were successfully prepared and showed sustained release in vitro and good ocular tolerance in vivo. This provides a promising alternative for treating chronic diseases of the posterior segment of the eye.
Article
Pharmacology & Pharmacy
Jose Javier Lopez-Cano, A. Sigen, Vanessa Andres-Guerrero, Hongyun Tai, Irene Bravo-Osuna, Irene Teresa Molina-Martinez, Wenxin Wang, Rocio Herrero-Vanrell
Summary: This study aimed to develop a thermo-responsive-injectable hydrogel based on PLGA-PEG-PLGA to deliver neuroprotective agents to the retina over time. Two PLGA-PEG PLGA copolymers with different ratios were synthesized and characterized, and HyG-1 was selected for further studies and loaded with anti-inflammatory drugs and antioxidants. In vitro drug release and cytotoxicity studies were performed, showing sustained release of drugs and good tolerance in retinal cells. The developed hydrogels represent a novel biodegradable tool for treating neurodegenerative processes in the retina.
Review
Cell & Tissue Engineering
Carlos M. Chiesa-Estomba, Ana Aiastui, Iago Gonzalez-Fernandez, Raquel Hernaez-Moya, Claudia Rodino, Alba Delgado, Juan P. Garces, Jacobo Paredes-Puente, Javier Aldazabal, Xabier Altuna, Ander Izeta
Summary: This study conducted a systematic review on the role of 3D bioprinting scaffolding for nasal cartilage defects repair, with a focus on cellular sources, types of scaffold materials, biochemical evaluation, histological analysis, in-vitro and in-vivo studies, and animal models used. The results showed high heterogeneity in mechanical evaluation, common patterns of collagen expression in scaffolds, and potential limitations in the translational investigation due to heterotopic scaffold implantation and lack of quantitative histological data. Further research is needed to address these issues in the growing field of 3D bioprinting for nasal cartilage defects repair.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Ophthalmology
Cristina Romo-Valera, Miguel Perez-Garrastachu, Raquel Hernaez-Moya, Maddalen Rodriguez-Astigarraga, Paula Romano-Ruiz, Jaime Etxebarria, Jon Arluzea, Noelia Andollo
Summary: The study found that even corneas conserved under long-term hypothermic conditions can still provide suitable LESCs. In limbal epithelial cell cultures, a simultaneous decrease in the melan A melanocyte marker and the putative stem cell markers was detected. There appears to be a close relationship between melanocytes and LSCs in the limbal stem cell niche.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Ophthalmology
J. J. Lopez-Cano, M. A. Gonzalez-Cela-Casamayor, V Andres-Guerrero, R. Herrero-Vanrell, J. M. Benitez-Del-Castillo, I. T. Molina-Martinez
Summary: This study investigated the effects of different osmolarities and ophthalmic therapeutic substances in human corneal epithelial cells and mouse macrophages. Results showed that Betaine, L-Carnitine, and Taurine can improve cell survival, while Hyaluronic acid is the most effective polymer in preventing cell death.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Marc Labetoulle, Jose Manuel Benitez-del-Castillo, Stefano Barabino, Rocio Herrero Vanrell, Philippe Daull, Jean-Sebastien Garrigue, Maurizio Rolando
Summary: This review describes the main categories of ingredients in tear substitutes and their effects on the ocular surface. It also provides insights into how to select the appropriate tear substitute formulations based on the predominant causes of dry eye disease (DED).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Biomaterials
M. C. Echave, I Erezuma, N. Golafshan, M. Castilho, F. B. Kadumudi, C. Pimenta-Lopes, F. Ventura, A. Pujol, J. J. Jimenez, J. A. Camara, R. Hernaez-Moya, L. Iturriaga, L. Saenz Del Burgo, I. Iloro, M. Azkargorta, F. Elortza, R. Lakshminarayanan, T. H. Al-Tel, P. Garcia-Garcia, R. Reyes, A. Delgado, C. Evora, J. L. Pedraz, A. Dolatshahi-Pirouz, G. Orive
Summary: In this study, a new osteoconductive scaffold was developed, which reduced the required doses of BMP-2 for bone regeneration. The scaffold showed promising results in promoting cell adhesion, proliferation, and osteogenic differentiation of human bone-marrow derived mesenchymal stem cells (hBM-MSCs). The sustained release of BMP-2 from the scaffold allowed for the treatment of critical-sized osteoporotic bone defects with relatively low growth factor doses.
BIOMATERIALS ADVANCES
(2022)
Article
Pharmacology & Pharmacy
Marco Brugnera, Marta Vicario-de-la-Torre, Vanessa Andres-Guerrero, Irene Bravo-Osuna, Irene Teresa Molina-Martinez, Rocio Herrero-Vanrell
Summary: This paper discusses the development and validation of a rapid method for the quantification of two neuroprotective agents in biodegradable PLGA microspheres. The method allows for the simultaneous determination of the two agents and has potential value in the treatment of glaucoma.
Article
Pharmacology & Pharmacy
Eduardo Anitua, Mar Zalduendo, Maria Troya, Itsasne Erezuma, Izeia Lukin, Raquel Hernaez-Moya, Gorka Orive
Summary: The study investigated the potential of three different bioinks based on gelatin and alginate for regenerative medicine. The bioinks containing hydroxyapatite and PRGF showed the highest cell compatibility and promoted osteogenic differentiation.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Ophthalmology
Ana Orive Banuelos, Begona Arana Larrea, Alja Crnej, Ana Arce Soto, Noelia Andollo Victoriano, Jaime Etxebarria Ecenarro
Summary: This article presents a surgical technique for performing TSCPC in BKPro II patients, and reports a case of a glaucoma patient with graft versus host disease who underwent the procedure. The results indicate that this treatment method is a safe, controlled, and effective option for patients with limited glaucoma treatment options. This is the first report describing the application of TSCPC surgical technique in BKPro II patients.
CASE REPORTS IN OPHTHALMOLOGY
(2022)
Article
Pharmacology & Pharmacy
Miriam Ana Gonzalez-Cela-Casamayor, Jose Javier Lopez-Cano, Irene Bravo-Osuna, Vanessa Andres-Guerrero, Marta Vicario-de-la-Torre, Manuel Guzman-Navarro, Jose Manuel Benitez-del-Castillo, Rocio Herrero-Vanrell, Irene Teresa Molina-Martinez
Summary: Glaucoma, a chronic irreversible neuropathy affecting the retina and optic nerve, is a major cause of blindness worldwide. Elevated intraocular pressure is a modifiable risk factor, and antihypertensive formulations are commonly used for treatment. However, frequent instillation of these formulations can lead to dry eye disease. In this study, liposome formulations loaded with brimonidine or travoprost, enriched with osmoprotective compounds, were developed. These formulations exhibited suitable physicochemical characteristics and encapsulation efficiency for ocular surface administration. They also showed good tolerability and osmoprotective activity in cell cultures. In animal experiments, the liposomal formulations demonstrated faster and longer-lasting reduction of intraocular pressure compared to commercial control products. Overall, the liposomal formulations combined with osmoprotective agents present a promising platform for the treatment of glaucoma and protection of the ocular surface.
Article
Orthopedics
Carlos M. Chiesa-Estomba, Raquel Hernaez-Moya, Claudia Rodino, Alba Delgado, Gonzalo Fernandez-Blanco, Javier Aldazabal, Jacobo Paredes, Ander Izeta, Ana Aiastui
Summary: In this study, the in vitro maturation of 3D-printed cell-laden polycaprolactone (PCL) scaffolds was improved, and their in vivo performance was tested in a rabbit auricular cartilage model. The results showed that scaffolds matured in vitro under prochondrogenic hypoxic conditions exhibited better mechanical properties and patterns of cartilage matrix deposition. This suggests that in vitro maturation can improve the functional properties of tissue-engineered grafts.
