Review
Green & Sustainable Science & Technology
Wenjing Sun, Mehdi Tajvidi, Christopher G. Hunt, Barbara J. W. Cole, Caitlin Howell, Douglas J. Gardner, Jinwu Wang
Summary: This review article summarizes the research and commercial applications of mycelium-based bio-composites. These materials can be produced by enzymatic or fungal treatment of lignocellulosic substrates, followed by hot-pressing into high-density panels. The article provides an overview of definitions, raw materials, processing procedures, material properties, and factors influencing product properties.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Microbiology
Anna Civzele, Alise Anna Stipniece-Jekimova, Linda Mezule
Summary: Lignocellulosic biomass is a valuable source for sustainable fuel and chemical production, but the degradation of lignin is challenging due to its complex structure. This study screened 10 fungal strains for ligninolytic enzyme production and identified four strains that can secrete ligninolytic enzymes under all experimental conditions, namely Irpex lacteus, Pleurotus dryinus, Bjerkandera adusta, and Trametes versicolor.
Article
Agricultural Engineering
Jyoti Rani, Abhishek S. Dhoble
Summary: In this study, the potential of two less explored fungal strains, Trichoderma longibrachiatum (TL) and Pycnoporus sanguineus (PS), in improving the hydrolysis and bioavailability of rice straw in anaerobic digestion (AD) was evaluated. The fungal treatment of rice straw for 10 days by TL and PS increased biogas production and reduced soluble chemical oxygen demand (sCOD). The microbial communities in treated samples showed increased diversity, contributing to better process performance and enhanced resilience against pH fluctuations.
BIORESOURCE TECHNOLOGY
(2023)
Article
Soil Science
Marta Gil-Martinez, Alvaro Lopez-Garcia, Maria T. Dominguez, Rasmus Kjoller, Carmen M. Navarro-Fernandez, Soren Rosendahl, Teodoro Maranon
Summary: Soil biodiversity loss due to pollution can be mitigated through phytoremediation, which enhances soil fungal diversity and functionality. Different tree species used in afforestation have species-specific effects on soil fungal diversity, with ectomycorrhizal fungi being more abundant under host trees. Phytoremediation leads to increased spatial heterogeneity in soil properties and recruitment of a wider variety of fungi, improving overall soil health and functionality.
SOIL BIOLOGY & BIOCHEMISTRY
(2021)
Article
Agricultural Engineering
Xiangyu Gu, Xiao Fu, Shulin Chen
Summary: The addition of Mn to fungal pretreatment of lignocellulosic biomass improves sugar production, particularly the recovery of hemicellulose sugars.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biotechnology & Applied Microbiology
Juliana Vasco-Correa, Rachel Capouya, Ajay Shah, Thomas K. Mitchell
Summary: A sequential fungal pretreatment of Miscanthus x giganteus using Ceriporiopsis subvermispora was conducted, showing enhanced cellulose digestibility in the first generation but no improvements in subsequent generations. High degradation of Miscanthus structural carbohydrates occurred in the first generation. Microbial community analysis revealed a decrease in the relative abundance of C. subvermispora, indicating the dominance of other microbes. Changes in the bacterial community may be associated with unwanted cellulose degradation in the system.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mohd Haffizi Hasni, Farah Binti Ahmad, Ahdyat Zain Athoillah
Summary: This study investigates the potential of using lignocellulosic biomass as a feedstock for microbial biodiesel production through consolidated bioprocessing. Rhizopus sp. was found to be the most effective lipid producer, and the optimized CBP system yielded biodiesel with promising fuel properties.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Soil Science
Johannes Ballauff, Dominik Schneider, Nur Edy, Bambang Irawan, Rolf Daniel, Andrea Polle
Summary: The conversion of diverse tropical lowland rainforests to monocultures of oil palm and rubber causes significant changes in fungal community structures. The effects of changes in root or soil properties on different trophic groups of fungi remain unknown. Root chemistry, especially the C/N ratio, strongly influences root-associated fungal communities, while root vitality affects soil-residing fungi. Changes in root and soil chemistry drive divergent responses in different functional groups and have a stronger impact on fungal community structures than geographic distance.
SOIL BIOLOGY & BIOCHEMISTRY
(2021)
Article
Environmental Sciences
Changjiang Huang, Xiaoqing Wu, Xiaoyu Liu, Yuting Fang, Lei Liu, Chuansheng Wu
Summary: The exposure situation and litter removal did not significantly affect wood decomposition, but there were differences among tree species. Fungal abundance had an impact on microbial respiration, and wood density was influenced by water content.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Chemical
Ludovica Varriale, Roland Ulber
Summary: Biorefineries convert lignocellulosic biomasses into a wide range of bioproducts, contributing to the transition from petrochemical resources to renewable ones. Organic acids, produced by microbials, have attracted attention for their utilization in various industrial areas and market growth. Filamentous fungi are becoming more popular for their ability to hydrolyze lignocellulosic biomasses and produce different organic acids, especially due to their capacity to ferment pentoses.
CHEMBIOENG REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Vivian Pascal Willers, Barbara Beer, Volker Sieber
Summary: Facing increasing mobility, energy demand, and the need to mitigate climate change, developing sustainable and eco-friendly alternatives to fossil fuels is a crucial task for humanity. Biofuels and synthetic fuels show great potential as they can be integrated into existing processes, with synthetic fuels from CO2 and renewable energies being considered the next big step towards a sustainable and ecological future. In our study, we focus on the sustainable production of ethanol and isobutanol, two common biofuels, using methanol and xylose derived from CO2 and lignocellulosic waste streams. Through enzyme and reaction optimization, we successfully produced ethanol or isobutanol from xylose and methanol. Our research opens up the possibility of hybrid production of biofuels and chemicals from CO2 and lignocellulosic waste streams, providing an alternative to conventional routes that rely solely on CO2 or sugars.
Article
Energy & Fuels
Waseem Ayoub Malik, Haris M. Khan, Saleem Javed
Summary: Cellulolytic enzymes produced by the bacterial strain Bacillus subtilis isolated from cow dung were optimized using response surface methodology and one-variable-at-a-time design, resulting in a threefold increase in cellulase production. The optimization process involved the significant contributions of galactose and sodium nitrate to enhance cellulase production.
BIOENERGY RESEARCH
(2022)
Article
Agricultural Engineering
Neha Srivastava, Rajeev Singh, Manish Srivastava, Akbar Mohammad, Steve Harakeh, Ravindra Pratap Singh, Dan Bahadur Pal, Shafiul Haque, Hossam H. Tayeb, Mohammed Moulay, Vijai Kumar Gupta
Summary: Biomass to biofuels production technology is a sustainable strategy for renewable energy resources. Pretreatment is a key step to improve cellulose availability and digestibility. Existing pretreatment technologies face challenges such as energy consumption, cost, removal of lignin, inhibitors production, and environmental pollution. The use of nanomaterials as nanocatalysts in pretreatment processes can overcome these limitations and improve biofuels production.
BIORESOURCE TECHNOLOGY
(2023)
Review
Agricultural Engineering
Zhengxin Wang, Xinggan Peng, Ao Xia, Akeel A. Shah, Yun Huang, Xianqing Zhu, Xun Zhu, Qiang Liao
Summary: The development and application of bioenergy and biofuels conversion technology are crucial for producing renewable and sustainable energy sources in the future. However, due to the complexity of bioenergy systems and human limitations in understanding, machine learning provides new opportunities. The latest advances in machine learning assisted bioenergy technology show great potential in driving the development of a new generation of bioenergy and biofuels conversion technologies in the future.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Environmental
Dendera Munguia-Aguilar, Hector A. Ruiz, Elias Razo-Flores, Cesar Nieto-Delgado, Edith Cadena-Chamorro, Felipe Alatriste-Mondragon
Summary: The efficiency of enzymatic saccharification of lignocellulosic biomass can be improved by a sequential process of hydrothermal pretreatment followed by enzymatic hydrolysis, resulting in higher release of sugars and methane production.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Review
Dentistry, Oral Surgery & Medicine
Erison Santana dos Santos, Joab Cabral Ramos, Ana Luiza Oliveira Correa Roza, Bruno Augusto Linhares Almeida Mariz, Adriana Franco Paes Leme
Summary: Osteopontin (OPN) is a calcium-binding glycol-phosphoprotein that is involved in various physiological and pathological processes, including oral cancer. Its activation of the PI3K/AKT/mTOR pathway contributes to the malignant phenotype of cancer cells, leading to cell proliferation, invasion, metastasis, angiogenesis, and resistance to treatment. Further research is needed to fully understand the role of OPN in oral cancer.
Article
Dentistry, Oral Surgery & Medicine
Reydson Alcides de Lima-Souza, Joao Figueira Scarini, Luccas Lavareze, Carolina Emerick, Livia Ramalho Crescencio, Romenia Ramos Domingues, Adriana Franco Paes Leme, Bruno Augusto Linhares Almeida Mariz, Debora Campanella Bastos, Renato Assis Machado, Alfio Jose Tincani, Andre Del Negro, Carlos Takahiro Chone, Luiz Paulo Kowalski, Erika Said Abu Egal, Albina Altemani, Fernanda Viviane Mariano
Summary: This study analyzed the proteomic profile of salivary pleomorphic adenoma (PA) and carcinoma ex pleomorphic adenoma (CXPA) samples, and identified seven potential protein signatures that may be associated with the acquisition of a malignant phenotype.
Article
Biotechnology & Applied Microbiology
Gustavo P. Lorca Mandujano, Henrique C. Alves, Cleiton D. Prado, Jeferson G. O. Martins, Hosana R. Novaes, Joao Pedro Maia de Oliveira da Silva, Gleidson Silva Teixeira, Andre Ohara, Mateus H. R. Alves, Isadora C. Pedrino, Iran Malavazi, Cristina Paiva de Sousa, Anderson F. da Cunha
Summary: This study reports the production of a Blond-style ale beer using a bioethanol isolated strain (LBGA-287) which showed improved maltose consumption, growth, and fermentation rates compared to commercial yeast. Genes related to fermentation efficiency, production of aromas/off-flavors, and metabolization of carbohydrates were found to be differentially expressed in LBGA-287, suggesting its potential impact on beer production.
Article
Biotechnology & Applied Microbiology
Allan H. F. de Melo, Alexia L. Nunes, Priscila H. Carvalho, Marcos F. da Silva, Gleidson S. Teixeira, Rosana Goldbeck
Summary: This research investigated the process of enzymatic saccharification and simultaneous fermentation to produce ethanol with sugarcane bagasse, using a modified strain of Saccharomyces cerevisiae. The results showed the potential of sugarcane bagasse and the modified strain in bioethanol production, providing useful insights for the development of SSF processes.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2023)
Review
Otorhinolaryngology
Erison Santana Dos Santos, Maria Eduarda Perez-de-Oliveira, Ana Gabriela Costa Normando, Luiz Alcino Monteiro Gueiros, Silvia Regina Rogatto, Pablo Agustin Vargas, Marcio Ajudarte Lopes, Eliete Neves da Silva Guerra, Adriana Franco Paes Leme, Alan Roger Santos-Silva
Summary: This study aimed to map the systemic alterations that predispose to the onset of oral squamous cell carcinoma. Data from multiple databases showed that certain systemic conditions, such as Fanconi's anemia and xeroderma pigmentosum, increase the risk of developing OSCC.
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2022)
Article
Microbiology
Fellipe da Silveira Bezerra de Mello, Alessandro Luis Venega Coradini, Marcelo Falsarella Carazzolle, Carla Maneira, Monique Furlan, Goncalo Amarante Guimaraes Pereira, Gleidson Silva Teixeira
Summary: This study evaluates the genetic architecture of industrial bioethanol yeast strain SA-1 and identifies genes related to aldehyde resistance and thermotolerance, providing important insights for improving yeast strains.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Ecology
Antonio P. Camargo, Rafael S. C. de Souza, Juliana Jose, Isabel R. Gerhardt, Ricardo A. Dante, Supratim Mukherjee, Marcel Huntemann, Nikos C. Kyrpides, Marcelo F. Carazzolle, Paulo Arruda
Summary: The grassland ecosystem of Brazilian campos rupestres has low concentrations of phosphorus and nitrogen, yet supports a high plant diversity. This study explores the taxonomic profile and functional potential of microbial communities associated with two plant species of the campos rupestres. The results show that the soil and rock communities associated with these plants share a core group of efficient colonizers enriched in certain bacterial families. The microbial populations associated with plant roots have a genetic repertoire for organic compound intake, phosphorus and nitrogen turnover, highlighting their role in nutrient availability.
Article
Biochemical Research Methods
Bianca A. Pauletti, Daniela C. Granato, Carolina M. Carnielli, Guilherme A. Camara, Ana Gabriela C. Normando, Guilherme P. Telles, Adriana F. Paes Leme
Summary: In this study, we developed a practical tool called Typic to assist in the selection of a list of proteotypic peptides for targeted quantitation. Typic integrates input data and downloads additional data to generate output files for each protein, which includes relevant information and auxiliary plots for peptide selection.
JOURNAL OF PROTEOME RESEARCH
(2023)
Letter
Dentistry, Oral Surgery & Medicine
Reydson Alcides de Lima-Souza, Joao Figueira Scarini, Luccas Lavareze, Romenia Ramos Domingues, Adriana Franco Paes Leme, Erika Said Abu Egal, Albina Altemani, Fernanda Viviane Mariano
Review
Biochemistry & Molecular Biology
Larissa Tino de Carvalho-Silva, Ana Gabriela C. Normando, Jamile de Oliveira Sa, Erison Santana dos Santos, Tatiane De Rossi, Ariane Fidelis Busso-Lopes, Ana Karina de Oliveira, Adriana F. Paes Leme
Summary: Cancer, a multifactorial disease, is a major cause of death that limits global life expectancy. The development and spread of cancer cells are influenced by the tumor microenvironment (TME), which consists of cancer cells, nonmalignant cells, and an intricate extracellular matrix. Various cell types and their secreted extracellular vesicles (EVs) in the TME, such as cancer-associated fibroblasts (CAFs), T lymphocytes, B cells, tumor-associated macrophages (TAMs), dendritic cells (DC), natural killer (NK) cells, tumor-associated neutrophils (TANs), stem cells, and endothelial cells, play a crucial role in cancer initiation and progression. This review highlights the importance of EVs from different populations of the TME in carcinogenesis.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Natalia G. Blanco, Natalia M. Machado, Ligia L. L. Castro, Mariana A. A. Antunes, Christina M. M. Takiya, Monique R. O. Trugilho, Luana R. R. Silva, Adriana F. Paes F. Leme, Romenia R. Domingues, Bianca A. A. Pauletti, Beatriz T. T. Miranda, Johnatas D. D. Silva, Claudia C. C. dos Santos, Pedro L. L. Silva, Patricia R. M. Rocco, Fernanda F. F. Cruz
Summary: The effects of different sources of MSC-derived extracellular vesicles (EVs), including those obtained from bone marrow (BM), adipose (AD), or lung (L) tissues, on sepsis were investigated. The proteome analysis of EVs revealed that BM-EVs were associated with less organ damage compared with AD-EVs and L-EVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Marisol Miranda-Galvis, Carolina Carneiro Soares, Carolina Moretto Carnielli, Jaqueline Ramalho Buttura, Raisa Sales de Sa, Estela Kaminagakura, Fabio Albuquerque Marchi, Adriana Franco Paes Leme, Clovis A. Lopes Pinto, Alan Roger Santos-Silva, Rogerio Moraes Castilho, Luiz Paulo Kowalski, Cristiane Helena Squarize
Summary: Human papillomavirus (HPV) infection has been linked to a subset of oral cavity cancers, but the molecular mechanisms underlying HPV-driven oral squamous cell carcinoma (OSCC) are not well understood. Proteomics profiling based on the HPV status in OSCC revealed differential abundance of proteins, including COPS3, DYHC1, S100A8, A2M, and Serpine1, which are associated with tumor recurrence, survival, and immune responses. HPV-positive OSCC displayed pathogen-associated pattern receptors, such as S100A8, and activated NF kappa B inflammatory responses. This peculiar microenvironment pattern has important implications for prognosis and treatment decisions.
Article
Biochemical Research Methods
Bianca A. Pauletti, Daniela C. Granato, Carolina M. Carnielli, Guilherme A. Camara, Ana Gabriela C. Normando, Guilherme P. Telles, Adriana F. Paes Leme
Summary: To address the challenge of selecting suitable proteotypic peptides for targeted quantitative proteomics assays, we have developed an open-source program called Typic. Typic is a practical and semiautomated workflow that combines input data with additional data from public repositories to select a list of proteotypic peptides. The program provides relevant information, a colored ranking of peptides, and auxiliary plots to assist users in the selection process. Overall, Typic allows for a more robust and standardized targeted proteomics assay.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Joao Paulo L. Franco Cairo, Fernanda Mandelli, Robson Tramontina, David Cannella, Alessandro Paradisi, Luisa Ciano, Marcel R. Ferreira, Marcelo Liberato, Livia B. Brenelli, Thiago A. Goncalves, Gisele N. Rodrigues, Thabata M. Alvarez, Luciana S. Mofatto, Marcelo F. Carazzolle, Jose G. C. Pradella, Adriana F. Paes Leme, Ana M. Costa-Leonardo, Mario Oliveira-Neto, Andre Damasio, Gideon J. Davies, Claus Felby, Paul H. Walton, Fabio M. Squina
Summary: Wood-feeding termites degrade plant biomass through enzymatic degradation, and a previously unknown redox-active enzyme called CgSOD-1 plays a key role in this process.
Article
Biotechnology & Applied Microbiology
Fellipe da Silveira Bezerra de Mello, Carla Maneira, Frank Uriel Lizarazo Suarez, Sheila Nagamatsu, Beatriz Vargas, Carla Vieira, Thais Secches, Alessando L. Coradini, Maria Augusta de Carvalho Silvello, Rosana Goldbeck, Goncalo Amarante Guimaraes Pereira, Gleidson Silva Teixeira
Summary: This study reports the CRISPR-Cas9 editing of major yeast strains in the Brazilian bioethanol industry for the first time. Fermentative assays revealed that NADPH availability is closely related to the performance of mutant strains in xylose consumption. The use of sugarcane straw as a substrate for xylitol production is also demonstrated for the first time. Industrially tolerant background SA-1 is shown to be a promising chassis for the second-generation industry, given its inhibitor tolerance and better redox environment that may favor the production of reduced sugars.
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
(2022)
