Journal
FOOD CHEMISTRY
Volume 281, Issue -, Pages 41-48Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.12.083
Keywords
Capillary electrophoresis; Fish; Kelp; On-line complexation; Organic mercury; Stacking
Funding
- Natural Science Foundation of Zhejiang Province, China [LY16H270018]
- Key projects of National Natural Science Foundation of China [81730108]
- Key Project of Zhejiang province Ministry of Science and Technology [2015C03055]
- Key Project of Hangzhou Ministry of Science and Technology [20162013A07, 20142013A63]
- Key Laboratory of Elemene Class Anticancer Chinese Medicine of Zhejiang Province
- Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province
Ask authors/readers for more resources
To determine organic mercury (Hg) species that could not be detected by ultraviolet (UV), a highly automated on-line complexation method was established, which combined with normal stacking by capillary electrophoresis-diode array detector. The approach was based on the fact that the compounds and complex reagent interacted to form hydrophilic chelates under the effect of the separation voltage, which was effectively separated and detected by UV. Key parameters, such as the type and concentration of complex reagent, separation voltage and so on were systematically investigated. Under the optimized conditions, the precision and repeatability were in the range of 0.16-3.31% and 0.17-1.21%, respectively. Furthermore, PhHg, EtHg and MeHg were effectively separated and determined in fresh fish (Silver carp) muscle and kelp (Kombu) with the recoveries of 84.63-111.39% and 75.68-114.76%, respectively. The proposed method had the advantages of easy-operating, cost-efficient, stable and reliable compared to off-line complexation method.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available