Article
Multidisciplinary Sciences
Liqiao Hu, Congcong Zhao, Mingjie Liu, Shuaiyu Liu, Jingjing Ye, Kehui Wang, Jinyun Shi, Wei Tian, Xiaojing He
Summary: CENP-I directly interacts with centromeric DNA and stabilizes the deposition of CENP-A. The DNA binding of CENP-I is crucial for the loading of CENP-A and the localization of centromeres.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Yu Nakabayashi, Masayuki Seki
Summary: The absence of TBS-III leads to reduced chromatin binding and total amount of centromere proteins, as well as up-regulated transcription at the centromere during M-phase. Ectopic transcription through the centromere displaces key centromere proteins, destabilizing the interaction between centromeres and microtubules, and causing defective chromosome segregation. The identification of new roles for histone binding residues in TBS-III provides new insights into nucleosome function during chromosome segregation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Review
Cell Biology
Alessandro Stirpe, Patrick Heun
Summary: Centromeres are specialized chromosome domains characterized by the epigenetic mark CENP-A. This review focuses on the establishment and maintenance of CENP-A during essential cellular processes, such as DNA repair, replication, and transcription, and highlights conserved mechanisms among different model organisms. It discusses the coordination and control of timely loading, maintenance, and removal of CENP-A and the gradually unraveled network of factors involved.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Charlene Renaud-Pageot, Jean-Pierre Quivy, Marina Lochhead, Genevieve Almouzni
Summary: CENP-A plays a critical role in chromosome segregation and genome integrity maintenance in mammals, but its overexpression is linked to cancer development. Studies have shown that CENP-A overexpression can affect genome integrity, cell fate, and nuclear organization in cancer, highlighting its potential as a target for cancer treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Min Zhang, Fan Zheng, Yujie Xiong, Chen Shao, Chengliang Wang, Minhao Wu, Xiaojia Niu, Fenfen Dong, Xuan Zhang, Chuanhai Fu, Jianye Zang
Summary: Our study demonstrates that the interaction of spMis18 with histone H2A-H2B and DNA is crucial for the recruitment of spMis18 and Cnp1 to the centromere in fission yeast. Mutations in the acidic region and deletion of the extreme C-terminus of spMis18 significantly affect its localization and delay chromosome segregation during mitosis, indicating the importance of these interactions in faithful chromosome segregation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Olivia Morrison, Jitendra Thakur
Summary: Chromatin is composed of DNA and histone proteins and is important for packaging DNA and regulating DNA metabolic pathways. It is categorized into euchromatin (active transcription), heterochromatin (silencing), and centromeric chromatin (chromosome segregation).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Andrew R. Popchock, Joshua D. Larson, Julien Dubrulle, Charles L. Asbury, Sue Biggins
Summary: Eukaryotic chromosome segregation requires a large machine called the kinetochore, which contains CENP-A and is responsible for nucleosome formation. This study investigates the functions of the chaperone protein HJURP in CENP-A deposition and the conservation of high AT DNA content at centromeres. Through a microscopy assay, it is found that CENP-A can arrive at centromeres without HJURP but stable incorporation requires HJURP and other DNA-binding proteins. Homopolymer AT runs in yeast centromeres are also essential for efficient CENP-A deposition. These findings provide insights into nucleosome formation and lay the foundation for future studies on kinetochore complexes.
Review
Biochemistry & Molecular Biology
Simon Leclerc, Katsumi Kitagawa
Summary: This article discusses the recent discovery of the function of human centromeric RNA in the regulation and structure of the centromere, as well as the consequences of dysregulation of centromeric RNA in cancer.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Genetics & Heredity
Na Wang, Jianing Liu, William A. Ricci, Jonathan Gent, R. Kelly Dawe
Summary: The research reveals a positive relationship between centromere size and genome size, independent of variations in major centromeric satellite sequences. Additionally, changes in centromere size involve alterations in bound CENH3, but overexpressing CENH3 does not mimic this effect. The study demonstrates that centromere size is among the scalable features affected by multiple limiting factors.
Article
Cell Biology
Koei Okazaki, Megumi Nakano, Jun-ichirou Ohzeki, Koichiro Otake, Kazuto Kugou, Vladimir Larionov, William C. Earnshaw, Hiroshi Masumoto
Summary: Human artificial chromosomes (HACs) are formed by introducing large centromeric sequences into cells, and the balance of chromatin states on the alphoid DNA is crucial for HAC formation. Our study explores the relationship between chromatin architecture and de novo HAC formation efficiency, and found that a combination of mutated and wild-type alphoid repeats enhances HAC formation.
Article
Biochemistry & Molecular Biology
Yujue Chen, Qian Zhang, Hong Liu
Summary: Non-coding centromeres, highly divergent in DNA sequences across species, are actively transcribed by RNAP II, facilitating CENP-A deposition and maintaining centromeric cohesion during mitosis. The regulation of centromeric transcription appears to be unique, as some traditional transcription initiation factors may not be required. This review discusses the novel role and regulation of centromeric transcription, as well as potential underlying mechanisms.
Article
Genetics & Heredity
Jessica R. Eisenstatt, Kentaro Ohkuni, Wei-Chun Au, Olivia Preston, Loran Gliford, Evelyn Suva, Michael Costanzo, Charles Boone, Munira A. Basrai
Summary: The mislocalization of overexpressed Cse4 is influenced by defects in proteolysis and sumoylation, with reduced dosage of histone H4 leading to suppression of CIN when Cse4 is overexpressed. This study identified genes that contribute to the mislocalization of Cse4 and highlighted the role of H4 in facilitating Cse4 sumoylation and mislocalization to noncentromeric regions.
Article
Biology
Yuting Liu, Kehui Wang, Li Huang, Jicheng Zhao, Xinpeng Chen, Qiang Wu, Zhouliang Yu, Guohong Li
Summary: The phosphorylation of CENP-A Ser68 is crucial for the cell-cycle-dependent deposition of CENP-A and cell viability.
SCIENCE CHINA-LIFE SCIENCES
(2022)
Review
Genetics & Heredity
Evon M. DeBose-Scarlett, Beth A. Sullivan
Summary: Centromeres are crucial for genome inheritance, with abnormal function linked to various diseases. Ectopic centromeres can form at new genomic locations, impacting genome stability and disease development. While certain genome regions influence neocentromere activation, the universal mechanism remains unclear.
ANNUAL REVIEW OF GENETICS, VOL 55
(2021)
Article
Multidisciplinary Sciences
Simona Giunta, Solene Herve, Ryan R. White, Therese Wilhelm, Marie Dumont, Andrea Scelfo, Riccardo Gamba, Cheng Kit Wong, Giulia Rancati, Agata Smogorzewska, Hironori Funabiki, Daniele Fachinetti
Summary: The study demonstrates the importance of the centromere-specific histone H3 variant CENP-A in safeguarding DNA replication of alpha-satellite repeats to prevent structural aneuploidy during S phase, providing insights into how specialized centromeric chromatin maintains the integrity of transcribed noncoding repetitive DNA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Y. H. Ling, C. C. Wong, K. W. Li, K. M. Chan, P. Boukamp, W. K. Liu
EXPERIMENTAL CELL RESEARCH
(2014)
Article
Plant Sciences
Mengli Chen, Florence W. K. Cheung, Ming Hung Chan, Pak Kwan Hui, Siu-Po Ip, Yick Hin Ling, Chun-Tao Che, Wing Keung Liu
JOURNAL OF ETHNOPHARMACOLOGY
(2012)
Article
Plant Sciences
Wing Keung Liu, Yick Hin Ling, Florence W. K. Cheung, Chun-Tao Che
JOURNAL OF NATURAL PRODUCTS
(2012)
Article
Chemistry, Medicinal
Florence W. K. Cheung, Jia Guo, Yick-Hin Ling, Chun-Tao Che, Wing-Keung Liu
Article
Multidisciplinary Sciences
Yick Hin Ling, Karen Wing Yee Yuen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)