Article
Pharmacology & Pharmacy
Irfan A. Rather, Mohammad Younus Wani, Majid Rasool Kamli, Jamal S. M. Sabir, Khalid Rehman Hakeem, Ahmad Firoz, Yong-Ha Park, Yan-Yan Hor
Summary: This study demonstrates the anti-biofilm and anti-virulence properties of Lactiplantibacillus plantarum KAU007 against Streptococcus pyogenes. The results show that Lactiplantibacillus plantarum KAU007 inhibits the growth and survival of S. pyogenes, as well as the formation of biofilm and the production of virulence-associated factors. This research provides a basis for further investigation into the use of Lactiplantibacillus plantarum KAU007 as an antibacterial agent to combat streptococcal infections.
Review
Medicine, General & Internal
Kate M. Miller, Timothy C. Barnett, Daniel Cadarette, David E. Bloom, Jonathan R. Carapetis, Jeffrey W. Cannon
Summary: The introduction of a Strep A vaccine could potentially reduce antibiotic prescribing for sore throat by 32-87% depending on changes to prescribing and consumption behaviors.
Review
Immunology
Shyra Wilde, Anders F. Johnson, Christopher N. LaRock
Summary: Group A Streptococcus is a major human pathogen with a natural tropism for the oropharynx and skin. Its virulence factors cause excessive inflammation, leading to severe infections, toxic shock syndrome, and post-infection autoimmune disease. Understanding how these factors induce inflammation and promote disease may lead to new therapeutic targets for severe infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Tulia Mateus, Elke J. Seppanen, Camilla de Gier, Sharon Clark, Harvey Coates, Shyan Vijayasekaran, Karen Prosser, Selma P. Wiertsema, Angela Fuery, Lea-Ann S. Kirkham, Peter C. Richmond, Ruth B. Thornton
Summary: This study investigated the bacterial load and species-specific densities in nasopharyngeal and oropharyngeal samples of children with pediatric obstructive sleep disordered breathing (SDB) and recurrent tonsillitis (RT). The presence of certain bacterial species was found to be associated with these conditions, suggesting that antimicrobials, especially anti-biofilm therapies, may be a strategy for managing these children.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Meredith B. Finn, Kathryn M. Ramsey, Simon L. Dove, Michael R. Wessels
Summary: The study reveals that the CsrRS system in Group A Streptococcus regulates gene expression by modulating the phosphorylation of CsrR, affecting the bacteria's ability to adapt to the human host. Analysis indicates that CsrR influences the expression of multiple genes by binding to regulated promoters and controlling the expression of intermediate transcription regulators.
Article
Ecology
Lijun Wang, Hongxia Wang, Hua Zhang, Hui Wu
Summary: Research has found that the commensal Streptococcus can enhance the biofilm formation ability of Staphylococcus, effectively promoting its colonization in vivo. The surface protein BapA1 of Streptococcus is crucial for the formation of the dual-species biofilm, while three virulence determinants of Staphylococcus play important roles in this process. These findings reveal a new mechanism in which the biofilm matrix formed between Streptococcus BapA1 and Staphylococcus eDNA contributes to the formation of the dual-species biofilm.
Article
Microbiology
H. Bergsten, L. M. Palma Medina, M. Morgan, K. Moll, D. H. Skutlaberg, S. Skrede, T. Wajima, M. Svensson, A. Norrby-Teglund
Summary: The study showed that adjunctive rifampin enhances the clearance of S. pyogenes biofilm and reduces its metabolic activity, extending the lag phase, as demonstrated in an in vitro tissue infection model.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Zehui Zhang, Yinfeng Liang, Lihui Yu, Menghan Chen, Yuru Guo, Zhiruo Kang, Chenghu Qu, Chunlian Tian, Dexian Zhang, Mingchun Liu
Summary: TatD DNases in Trueperella pyogenes are involved in biofilm formation and contribute to virulence during infections, as demonstrated by reduced biofilm thickness, biomass, bacterial load, and compromised virulence in tatD-deficient mutants compared to the wild-type strain.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaolan Zhang, Yue Wang, Hui Zhu, Zhaohua Zhong
Summary: This study focuses on the role of an esterase (Sse) secreted by Streptococcus pyogenes in pathogenesis. By generating a gene knockout strain and conducting transcriptome analysis, the study reveals that Sse plays a crucial role in GAS virulence and metabolic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Wenhua Huang, Ying Chen, Qian Li, Hua Jiang, Qingyu Lv, Yuling Zheng, Xuelian Han, Decong Kong, Peng Liu, Yongqiang Jiang
Summary: The study revealed that the LytR protein influences the cell morphology, biofilm formation, and virulence of Streptococcus suis (S. suis), playing crucial roles during infection.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Dag Harald Skutlaberg, Harald G. Wiker, Haima Mylvaganam, Anna INFECT Study Grp, Anna Norrby-Teglund, Steinar Skrede
Summary: Biofilm formation has been observed in muscle and soft tissue samples from patients with necrotizing soft tissue infection caused by Streptococcus pyogenes. However, the clinical importance of this observation is unclear. This study characterized the biofilm forming capacity in a collection of S. pyogenes isolates causing Necrotizing Soft Tissue Infection (NSTI) and investigated its association with emm type and clinical characteristics of the patients. The results showed that the biofilm forming capacity of emm1 isolates was different from other emm types, but there was no significant association between biofilm forming capacity and clinical characteristics of the patients. The impact of biofilm formation in NSTI caused by S. pyogenes on clinical outcomes remains uncertain.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Dingle Yu, Waiwai Gao, Danchun Guo, Qinghua Lu, Yunsheng Chen, Yuejie Zheng, Wenjian Wang, Yonghong Yang
Summary: This study retrospectively analyzed the clinical characteristics and treatment of septic arthritis caused by Streptococcus pyogenes in three pediatric cases. The findings suggest that early diagnosis and timely treatment, including abscess incision and drainage followed by culture of the abscess puncture fluid, are important for children with septic arthritis. Beta-lactam antibiotics provide effective treatment once S. pyogenes infection is confirmed, avoiding the use of broad-spectrum antibiotics.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Yang Zhou, Zhuoying Liu, Jie Wen, Yan Zhou, Huancai Lin
Summary: This study found that berberine chloride hydrate (BH) effectively suppresses the biofilm formation and cariogenic virulence of Streptococcus mutans (S. mutans). The inhibitory effect of BH is reduced under acidic condition and elevated under alkaline condition. BH can maintain good structural stability and low toxicity to erythrocytes at different pH values. Furthermore, BH downregulates the expression of certain genes related to adhesion and biofilm formation in S. mutans.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Yang Zhou, Zhuoying Liu, Jie Wen, Yan Zhou, Huancai Lin
Summary: This study investigated the antibiofilm activities of berberine chloride hydrate (BH) against Streptococcus mutans biofilm. The results showed that BH effectively suppressed S. mutans biofilm formation and cariogenic virulence. The inhibitory effect was influenced by pH values, with a reduced effect under acidic condition and an elevated effect under alkaline condition. BH maintained good structural stability and low toxicity to erythrocytes. The downregulation of critical genes involved in adhesion, bacterial colonization, and acid production explained the inhibitory effect of BH on S. mutans biofilm formation.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Fabrice Jean-Pierre, Arsh Vyas, Thomas H. Hampton, Michael A. Henson, George A. O'Toole
Summary: Culture-independent studies have shown that chronic lung infections in persons with cystic fibrosis involve diverse microbial communities. Interactions among these communities influence clinical outcomes, and a single community cannot fully explain the diversity of outcomes. Combining computational and experimental approaches can help enhance our understanding of microbial community function and lead to the identification of new therapeutics for polymicrobial infections.
Article
Immunology
Jonathan G. Williams, Diane Ly, Nicholas J. Geraghty, Jason D. McArthur, Heema K. N. Vyas, Jody Gorman, James A. Tsatsaronis, Ronald Sluyter, Martina L. Sanderson-Smith
Summary: Invasive infections caused by group A Streptococcus (GAS) can rapidly progress due to tissue degradation and unregulated inflammation. Neutrophils are the primary immune cells that respond to GAS. Infections with strains such as 5448AP that promote an inflammatory neutrophil phenotype may contribute to increased inflammation and ineffective bacterial eradication, worsening the severity of invasive GAS infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Heema K. N. Vyas, Jason D. McArthur, Martina L. Sanderson-Smith
Summary: Group A Streptococcus (GAS) causes 700 million infections and accounts for half a million deaths per year. This study evaluates biofilm formation by GAS and its correlation with multiple virulence factors, as well as optimizing a host cell-GAS model for long-term biofilm formation. The 72-hour growth period is proposed as optimal for detecting reproducible biofilm biomass, which closely resembles in vivo structures.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Wai Leong, Wee Han Poh, Jonathan Williams, Carla Lutz, M. Mozammel Hoque, Yan Hong Poh, Benny Yeo Ken Yee, Cliff Chua, Michael Givskov, Martina Sanderson-Smith, Scott A. Rice, Diane McDougald
Summary: This study reveals the evolutionary selection effects of protozoa on Pseudomonas aeruginosa, leading to attenuated pathogenicity and enhanced fitness in amoeba-adapted isolates, similar to those observed in chronic cystic fibrosis infections.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
T. B-D Mcewan, R. A. Sophocleous, P. Cuthbertson, K. J. Mansfield, M. L. Sanderson-Smith, R. Sluyter
Summary: The study revealed that scratched cells release adenosine 5'-triphosphate (ATP) which activates P2Y(2) receptors to facilitate wound closure in HaCaT keratinocytes. Inhibition of ATP release and P2Y(2) receptor activation impaired wound healing, highlighting the importance of autocrine purinergic signaling in this process.
Article
Multidisciplinary Sciences
Jennifer West, Sandeep Satapathy, Daniel R. Whiten, Megan Kelly, Nicholas J. Geraghty, Emma-Jayne Proctor, Pietro Sormanni, Michele Vendruscolo, Joel N. Buxbaum, Marie Ranson, Mark R. Wilson
Summary: Neuroserpin has the ability to inhibit amyloid formation by multiple proteins and protect against associated cytotoxicity. It shows unique chaperone selectivity for intermediates on the amyloid-forming pathway, but has poor ability to inhibit amorphous protein aggregation. A conserved 14-residue region containing an alpha helix is identified, which may be useful in designing anti-amyloid reagents in the future.
Article
Microbiology
Samantha Ognenovska, Chinmoy Mukerjee, Martina Sanderson-Smith, Kate H. Moore, Kylie J. Mansfield
Summary: This study aimed to determine the virulence mechanisms that enable uropathogens to invade urothelial cells. The findings showed that common uropathogens were capable of intracellular colonization and the expression of virulence mechanisms correlated with successful invasion. These findings suggest that bacterial reservoirs within the bladder may contribute to the incidence of recurrent UTIs.
Article
Infectious Diseases
Caitlin Keighley, Antoine M. van Oijen, Stuart J. Brentnall, Martina Sanderson-Smith, Peter Newton, Spiros Miyakis
Summary: The study found similar trends in antimicrobial resistance (AMR) among urinary Escherichia coli isolates from a private community-based laboratory and a public hospital-based laboratory in an Australian local health district. AMR rates increased over time in both settings, with consistently higher rates in the public hospital-based laboratory. Interventions targeting the community-based laboratory setting are crucial for addressing AMR in the community.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Immunology
Jonathan G. Williams, Ronald Sluyter, Martina L. Sanderson-Smith
Summary: This study characterized the human neutrophil response to Streptococcus pyogenes using in vitro experiments. The results showed that neutrophil antimicrobial defense against GAS was reduced during early infection, neutrophil death was delayed, and infection induced inflammation. Different strains of GAS also showed differential expression of cell-surface proteins.
Article
Microbiology
Joanna G. G. Rothwell, Jungmi Hong, Stuart J. J. Morrison, Heema Kumari Nilesh Vyas, Binbin Xia, Anne Mai-Prochnow, Robyn McConchie, Kim-Yen Phan-Thien, Patrick J. J. Cullen, Dee A. A. Carter
Summary: Plasma-activated water (PAW) is a competitive sanitizer technology in the fresh food industry, achieving significant reduction of pathogens and spoilage microorganisms while maintaining the quality and shelf life of the produce item.
MICROBIOLOGY SPECTRUM
(2023)
Article
Polymer Science
Zhongyan Li, Matthew Crago, Timothy Schofield, Haoxiang Zeng, Heema Kumari Nilesh Vyas, Markus Mullner, Anne Mai-Prochnow, Syamak Farajikhah, Sina Naficy, Fariba Dehghani, Sepehr Talebian
Summary: In this study, a flexible pH-responsive hydrogel was developed by functionalizing hydrophilic polyurethanes with either cationic or anionic moieties. The physicochemical characterization of the resulting polymers was carried out, and the loading of the hydrogels with various compound models showed that the electrostatic forces between the polymeric backbone and the compound model determined the release rate at any given pH. The potential application of these hydrogels for chronic wound drug delivery was assessed and showed different degrees of antibacterial effect. Overall, cationic and anionic hydrophilic polyurethanes show potential as flexible pH-responsive materials for therapeutic delivery to chronic wounds. Rating: 8/10.
Article
Engineering, Environmental
Binbin Xia, Heema Kumari Nilesh Vyas, Renwu Zhou, Tianqi Zhang, Jungmi Hong, Joanna G. Rothwell, Scott A. Rice, Dee Carter, Kostya (Ken) Ostrikov, Patrick J. Cullen, Anne Mai-Prochnow
Summary: Microbial biofilms cause contaminations and pose risks to human health. Cold plasma and plasma-activated water (PAW) have disinfectant activity, but the best gas choice for generating PAW is unclear.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Microbiology
Heema Kumari Nilesh Vyas, Binbin Xia, Anne Mai-Prochnow
Summary: This review discusses innovative in vitro models that better mimic the environments of clinically important biofilm-associated infections, and highlights their contribution to translating laboratory findings into clinical practice.
Review
Microbiology
Anuk D. Indraratna, Arun Everest-Dass, Danielle Skropeta, Martina Sanderson-Smith
Summary: This article reviews the role of human glycans in the infection process of Group A Streptococcus and highlights the clinical value of understanding glycointeractions in combating GAS disease.
FEMS MICROBIOLOGY REVIEWS
(2022)
