Review
Biochemistry & Molecular Biology
Alexander Bernhard Rister, Thomas Gudermann, Johann Schredelseker
Summary: The voltage-dependent anion channel (VDAC) is a main pathway for ions and metabolites across the outer mitochondrial membrane, and its regulation involves various cellular factors and molecular partners. Its specific roles and binding sites, especially the residue E73, are still largely unknown.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Leenu Reinsalu, Marju Puurand, Vladimir Chekulayev, Sten Miller, Igor Shevchuk, Kersti Tepp, Egle Rebane-Klemm, Natalja Timohhina, Anton Terasmaa, Tuuli Kaambre
Summary: Metabolic plasticity is the ability of cells to adjust metabolism to environmental changes. Cancer cells exhibit increased metabolic plasticity, allowing them to have a survival advantage and higher proliferative capacity. This enhanced plasticity is characterized by changes in adenine nucleotide transport, impaired regulation of VDAC and reprogrammed energy transfer circuits like AK and CK pathways.
FRONTIERS IN ONCOLOGY
(2021)
Article
Neurosciences
Xiaofan Ning, Xinyue Li, Kai Ma, Huan Pang, Tiantian Tian, Huifang Hao, Qiaohong Hou, Meng Li, Tianxiang Liu, Shanshan Hou, Haiying Du, Xiuling Song, Zhiwei Sun, Chao Zhao, Minghua Jin
Summary: Silica nanoparticles are widely used in industry, electronics, and pharmaceutical industries, as well as medicine, tumor treatment and diagnosis, and other biomedical and biotechnology fields. The increasing exposure to silica nanoparticles through iatrogenic, occupational, and environmental sources has raised concerns about their potential damage and effects on the nervous system.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Victor V. Lemeshko
Summary: Recently reported kinase-linked mild depolarization of mitochondria may be a crucial component of the mitochondrial anti-aging program. However, the current measurement methods for mitochondrial inner membrane potential ignore the possibility of outer membrane potential generation. The experimental data reported in this study may reflect changes in the positive outer membrane potential generated by VDAC-kinase complexes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Review
Physiology
Varda Shoshan-Barmatz, Uttpal Anand, Edna Nahon-Crystal, Marta Di Carlo, Anna Shteinfer-Kuzmine
Summary: Metformin has been used to treat diabetes and other diseases since the late 1950s. Despite its opposite effects on different diseases, its involvement with VDAC1 suggests that it may be a key factor in metformin's impact on various diseases.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biology
Ivanka Kostadinova, Magdalena Kondeva-Burdina, Lyubomir Marinov, Lubomir L. L. Vezenkov, Rumyana Simeonova
Summary: Oxidative stress is a key factor in many diseases. Newly synthesized creatine derivatives have antioxidant and neuroprotective effects, making them potential therapeutic agents for neurodegenerative disorders.
Article
Cell Biology
Filomena Broeskamp, Elizabeth S. M. Edrich, Oskar Knittelfelder, Lisa Neuhaus, Thorsten Meyer, Jonas Heyden, Lukas Habernig, Florian Kreppel, Campbell W. Gourlay, Patrick Rockenfeller
Summary: This study investigated the impact of mitochondrial outer membrane protein Por1 on yeast autophagy, showing that POR1 deficiency reduces autophagic capacity and alters organelle and lipid homeostasis. It was found that the reduced autophagy caused by POR1 deficiency could be circumvented by overexpression of Psd1, suggesting a role for Por1 in Psd1-mediated autophagy regulation.
Article
Biology
Sven Dennerlein, Sabine Poerschke, Silke Oeljeklaus, Cong Wang, Ricarda Richter-Dennerlein, Johannes Sattmann, Diana Bauermeister, Elisa Hanitsch, Stefan Stoldt, Thomas Langer, Stefan Jakobs, Bettina Warscheid, Peter Rehling
Summary: TMEM223 and SMIM4 were identified as ribosome-associated proteins involved in complex IV and III biogenesis, respectively. They stimulate mRNA translation and support early assembly steps, linking translation machinery to the biogenesis of OXPHOS complexes.
Article
Multidisciplinary Sciences
Catherine A. Makarewich, Amir Z. Munir, Svetlana Bezprozvannaya, Aaron M. Gibson, Soo Young Kim, Misty S. Martin-Sandoval, Thomas P. Mathews, Luke I. Szweda, Rhonda Bassel-Duby, Eric N. Olson
Summary: New evidence suggests that certain noncoding RNA molecules actually code for microproteins. By using comparative genomics, researchers identified a microprotein called Mtlbn that is abundantly expressed in the heart. Mtlbn localizes to the inner mitochondrial membrane and interacts with subunits of the electron transport chain and respiratory supercomplexes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Olga A. Averina, Oleg A. Permyakov, Mariia A. Emelianova, Olga O. Grigoryeva, Maxim L. Lovat, Anna E. Egorova, Andrei V. Grinchenko, Vadim V. Kumeiko, Maria V. Marey, Vasily N. Manskikh, Olga A. Dontsova, Mikhail Yu. Vysokikh, Petr V. Sergiev
Summary: Small peptides are important components of mitochondrial proteome, and the mitochondrial peptide Mtln plays a crucial role in respiratory complex I functioning and other processes in mitochondria. Knockout of Mtln in mice leads to obesity, triglyceride accumulation, and depletion of tricarboxylic acids cycle intermediates, indicating its important physiological functions. In addition, Mtln deficiency also impairs muscle strength, disrupts mitochondrial cardiolipin balance, and affects respiratory chain performance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Arvinder Kapur, Pooja Mehta, Aaron D. Simmons, Spencer S. Ericksen, Geeta Mehta, Sean P. Palecek, Mildred Felder, Zach Stenerson, Amruta Nayak, Jose Maria Ayuso Dominguez, Manish Patankar, Lisa M. Barroilhet
Summary: The Warburg effect, characterized by high glucose uptake and lactate release, is a hallmark of most cancers. Atovaquone, a mitochondrial complex III inhibitor, has been found to slow ovarian cancer growth and inhibit the proliferation of cancer stem cells both in vitro and in vivo. The drug is able to inhibit oxygen consumption and ATP production, and it alters several metabolic pathways. These findings provide mechanistic insights and preclinical data to support further investigation of atovaquone as a potential therapy for gynecologic cancers.
Review
Cell Biology
Joyce T. Varughese, Susan K. Buchanan, Ashley S. Pitt
Summary: Voltage-dependent anion channel (VDAC) is a beta-barrel membrane protein located in the outer mitochondrial membrane, playing major roles in regulating various physiological processes and contributing to the development of diseases.
Article
Neurosciences
Liam F. Fitzgerald, Miles F. Bartlett, Rajakumar Nagarajan, Ericber Jimenez Francisco, Frank C. Sup, Jane A. Kent
Summary: Muscle metabolic economy (ME) is similar in young and older adults during some isometric contractions, but age-related differences exist during dynamic contractions. Older adults have lower ME during dynamic contractions, despite similar muscle oxidative capacity and ATP flux compared to younger adults. This suggests a dissociation between cross-bridge activity and ATP utilization with age.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Review
Cell Biology
Katarzyna Glombik, Jan Detka, Boguslawa Budziszewska
Summary: Hormones play a crucial role in regulating neuronal and synaptic plasticity, neurogenesis, and metabolic activity in the brain, ensuring proper communication between the brain and the body. The influence of hormones on energy metabolism and mitochondrial function in the brain has gained significant attention, as excess or deficiency of hormones is associated with cell damage and loss of function in mitochondria.
Article
Medical Laboratory Technology
Vishnu Suresh Babu, Ashwin Mallipatna, Gagan Dudeja, Rohit Shetty, Archana Padmanabhan Nair, Sai Bo Bo Tun, Candice Ee Hua Ho, Shyam S. Chaurasia, Shomi S. Bhattacharya, Navin Kumar Verma, Rajamani Lakshminarayanan, Nilanjan Guha, Stephane Heymans, Veluchamy Amutha Barathi, Arkasubhra Ghosh
Summary: Lack of Rb protein in Rb tumors causes altered metabolism and enhanced sensitivity to chemotherapy drugs. Restoring HK1 or RB1 in these cells reduces cancer hallmarks and increases sensitivity to treatment. Activation of AMPKα also enhances the tumoricidal effects of chemotherapy drugs.
TRANSLATIONAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Ljudmila Klepinina, Aleksandr Klepinin, Laura Truu, Vladimir Chekulayev, Heiki Vija, Kaisa Kuus, Indrek Teino, Martin Pook, Toivo Maimets, Tuuli Kaambre
Summary: The study demonstrates that modulating cell metabolism through NaBT can be an effective strategy for treating colorectal cancer (CRC), with significant impacts on the energy metabolism and differentiation state of CRC cells.
Article
Biochemistry & Molecular Biology
Song Zhang, Satsuki Yamada, Sungjo Park, Aleksandr Klepinin, Tuuli Kaambre, Andre Terzic, Petras Dzeja
Summary: The study revealed that AK2 plays a crucial role in early embryonic development and its loss in adults can lead to sudden heart failure, which is eventually recovered with an adaptive response. The compensatory upregulation of related enzymes and genes highlights the innate plasticity of the AK2-integrated bioenergetics system in maintaining homeostasis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Oncology
Leenu Reinsalu, Marju Puurand, Vladimir Chekulayev, Sten Miller, Igor Shevchuk, Kersti Tepp, Egle Rebane-Klemm, Natalja Timohhina, Anton Terasmaa, Tuuli Kaambre
Summary: Metabolic plasticity is the ability of cells to adjust metabolism to environmental changes. Cancer cells exhibit increased metabolic plasticity, allowing them to have a survival advantage and higher proliferative capacity. This enhanced plasticity is characterized by changes in adenine nucleotide transport, impaired regulation of VDAC and reprogrammed energy transfer circuits like AK and CK pathways.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Aleksandr Klepinin, Sten Miller, Indrek Reile, Marju Puurand, Egle Rebane-Klemm, Ljudmila Klepinina, Heiki Vija, Song Zhang, Andre Terzic, Petras Dzeja, Tuuli Kaambre
Summary: The dynamics of ATP phosphoryl turnover and metabolic flux through phosphotransfer pathways in cancer cells are not well understood. In this study, using O-18 phosphometabolite tagging technology, the authors investigated the phosphotransfer dynamics in three breast cancer cell lines and found a decreased turnover of γ- and β-ATP in cancer cells compared to control. They also observed reduced fluxes through HK, CK, and mitochondrial substrate shuttle in aggressive cancer cells. The downregulation of MTCK1A was linked to the decreased CK metabolic flux. However, overexpression of HK2, AK2, and AK6 isoforms in cell compartments could promote aggressive breast cancer growth.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biology
Marju Puurand, Kersti Tepp, Tuuli Kaambre
Summary: This article summarizes the use of metabolic control analysis (MCA) and permeabilized cells and tissues to study the oxidative phosphorylation (OXPHOS) system in cancer cells. Examples of using different approaches and methodologies in cancer research are also provided.
Article
Biochemistry & Molecular Biology
Kersti Tepp, Jekaterina Aid-Vanakova, Marju Puurand, Natalja Timohhina, Leenu Reinsalu, Karin Tein, Mario Plaas, Igor Shevchuk, Anton Terasmaa, Tuuli Kaambre
Summary: This study aimed to investigate the role of Wfs1 deficiency on muscle bioenergetics. The results revealed that Wfs1 gene mutation led to a decrease in oxygen consumption rate in muscles, with substrate-dependent alterations. Additionally, it was found that the respiration rate of muscles significantly decreased during the development of WS, although other changes were relatively minor.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Correction
Chemistry, Analytical
Karl Kristjan Kaup, Lauri Toom, Laura Truu, Sten Miller, Marju Puurand, Kersti Tepp, Tuuli Kaambre, Indrek Reile
Article
Chemistry, Analytical
Karl Kristjan Kaup, Lauri Toom, Laura Truu, Sten Miller, Marju Puurand, Kersti Tepp, Tuuli Kaambre, Indrek Reile
Summary: In this study, a combination of established pure shift NMR and artifact suppression techniques was used to record P-31 pure shift NMR spectra without extending measurement times or causing line broadening. This approach successfully resolved overlapping multiplets of homonuclear coupled phosphorus nuclei, demonstrating its potential for phosphometabolomics analysis.
