Article
Medicine, General & Internal
Dominika Peskar, Tadeja Kuret, Jera Jeruc, Andreja Erman
Summary: Studying changes in tissue glycosylation patterns may help discover biomarkers and therapeutic targets for IC/BPS. We compared glycosylation patterns of human urothelium between normal individuals and IC/BPS patients, as well as in experimental models of IC/BPS. Our results suggest potential biomarkers for histopathological diagnosis of IC/BPS and provide an adequate platform for preclinical study of IC/BPS glycobiology.
Article
Biochemistry & Molecular Biology
Dominika Peskar, Tadeja Kuret, Katja Lakota, Andreja Erman
Summary: Animal models were used to study the pathophysiology of IC/BPS. The mouse model induced with low-dose CYP treatment showed upregulation of inflammatory transcripts and activation of the JAK3/STAT3 pathway in bladder cells. Gender differences were observed in cell proliferation and immune responses. This study provides valuable insights into IC/BPS research and the sex-specific mechanisms involved.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Pedro Abreu-Mendes, Aurora Costa, Ana Charrua, Rui Almeida Pinto, Francisco Cruz
Summary: There is a trend towards a significant association between increased VEGF levels and pain or symptom severity in BPS/IC patients. Although there are some discrepancies among the studies and the number of patients included is small, VEGF and its receptors should be considered for future studies regarding its use in BPS/IC pathophysiology, diagnosis, and prognostication.
Review
Medicine, General & Internal
Jochen Neuhaus, Mandy Berndt-Paetz, Andreas Gonsior
Summary: IC/BPS is a complex disease with various factors involved such as genetic alterations, impaired angiogenesis, and microbial infections. Environmental, nutritional, and infectious factors may influence the development of IC/BPS. Advancements in molecular biology techniques promise a more sophisticated analysis of the pathophysiological network, leading to a better understanding of IC/BPS and related diseases.
Article
Urology & Nephrology
Nagendra N. Mishra
Summary: This study retrospectively reviewed the experience and treatment results of 380 patients diagnosed with IC/BPS at a clinic in India over the past 20 years. The results showed that 67% of patients had notable improvements, 13% had moderate improvements, and 18% showed no improvement. Surgery was found to be effective in improving symptoms, but it is recommended as a last resort.
NEUROUROLOGY AND URODYNAMICS
(2022)
Article
Urology & Nephrology
Jung Hyun Shin, Bosik Kang, Myung-Soo Choo
Summary: The study found that the features of HLs in IC patients were not associated with subjective symptoms, but inflammatory changes in HLs were a predictive factor for IC recurrence and associated with frequent urgency episodes and smaller bladder capacity.
JOURNAL OF UROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tadeja Kuret, Mateja Erdani Kreft, Rok Romih, Peter Veranic
Summary: This study investigated the potential effect of cannabidiol (CBD) in alleviating the symptoms of interstitial cystitis/bladder pain syndrome (IC/BPS). The results showed that CBD treatment significantly reduced inflammation and oxidative stress in urothelial cells, highlighting its potential therapeutic importance for IC/BPS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Zhinoos Taidi, Tommy Zhou, Kate H. Moore, Kylie J. Mansfield, Lu Liu
Summary: Inflammatory conditions of the urinary bladder are associated with urothelial damage and loss of function. The purinergic P2X7 receptor plays a role in such inflammatory conditions and may be a new therapeutic target for bladder inflammation.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jin Li, Xianyanling Yi, Jianzhong Ai
Summary: Interstitial cystitis/bladder pain syndrome (IC/BPS) is a debilitating disease with a negative impact on quality of life. Diagnoses and treatments are still unsatisfactory, calling for new approaches. This study aims to provide a comprehensive overview of IC/BPS research, promoting progress in diagnosis, treatment, and prognosis. The complexity of IC/BPS and emerging evidence call for further studies to optimize management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuan-Hong Jiang, Jia-Fong Jhang, Yuan-Hsiang Hsu, Hann-Chorng Kuo
Summary: This study investigated the usefulness of urinary biomarkers in assessing bladder condition and histopathology in IC/BPS patients. The results show that urinary biomarker levels are associated with symptoms and pathological manifestations, helpful in identifying HIC and NHIC subtypes, but most biomarkers are not strongly correlated with specific bladder histopathological findings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yuan-Hong Jiang, Jia-Fong Jhang, Lori A. A. Birder, Hann-Chorng Kuo
Summary: The aim of this study was to investigate the expression levels of sensory receptors, inflammatory proteins, and pro-apoptotic proteins in the urothelium of non-Hunner's interstitial cystitis (NHIC) bladders of patients with different clinical and cystoscopic phenotypes. The study concluded that IC/BPS patients with frequency and bladder pain complaints have higher levels of urothelial sensory receptors, and inflammatory and pro-apoptotic proteins. The expression levels of these sensory receptors, inflammatory proteins, and pro-apoptotic proteins are not significantly different among IC/BPS bladders with different conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Maryam Fatima, Irfan Anjum, Aamir Abdullah, Shaun Zshaan Abid, Muhammad Nasir Hayat Malik
Summary: The study demonstrates the protective effect of boswellic acids on cyclophosphamide-induced cystitis in rats, indicating their potential as therapeutic alternatives for cystitis. Boswellic acids exhibit antioxidant and anti-inflammatory properties, reducing urothelial damage and inflammation.
Review
Urology & Nephrology
Winfried Vahlensieck, Raschid El Idrissi, Ursula Gerhard-Franke, Maren Huber, Jennifer Jenner, Dirk-Henrik Zermann
Summary: According to the German IC/BPS guideline, after unsuccessful outpatient conservative treatment, invasive inpatient treatment, or cystectomy in IC/BPS patients, a discipline-specific inpatient urological rehabilitation should be proposed. The rehabilitation process includes completing diagnostic tests, optimizing lifestyle and medication, intensive psychotherapy, various forms of exercise therapy and physical therapy, such as water applications, thermotherapy, massages, and electrical/magnetic therapies, as well as nutritional counseling. Most patients show significant improvement after rehabilitation, and about 50% experience prolonged relief of symptoms.
Article
Medicine, Research & Experimental
Robert M. Moldwin, Vishaan Nursey, Oksana Yaskiv, Siddhartha Dalvi, Eric J. Macdonald, Michael Funaro, Chengliang Zhang, William DeGouveia, Marina Ruzimovsky, Horacio R. Rilo, Edmund J. Miller, Souhel Najjar, Inna Tabansky, Joel N. H. Stern
Summary: Interstitial cystitis/bladder pain syndrome patients with Hunner lesions (HL) show local inflammatory events and increased levels of immune cells. Urinary levels of different cytokines are also elevated in HL patients, indicating different disease processes compared to patients without HL.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Physical
Q. Sun, Q. Y. Zhang, N. Zhou, L. Y. Zhang, Q. Hu, C. Y. Ma
Summary: This study investigated the extinction spectra and local electromagnetic fields of Ag-decorated ZnO nanorods using the FDTD method, revealing the significant impact of different parameters on Raman enhancement. The findings are valuable for the design and optimization of 3D SERS substrates.
APPLIED SURFACE SCIENCE
(2021)
Article
Engineering, Biomedical
Sanghwa Lee, Jung-Man Namgoong, Youngkyu Kim, Jaepyeong Cha, Jun Ki Kim
Summary: This study developed a real-time surgical guidance system that combines multiple optical imaging techniques, including visible imaging, hyperspectral imaging, and laser speckle contrast imaging, to monitor blood vessel perfusion, oxygen saturation, thrombosis, and tissue recovery. The system was demonstrated by clamping blood vessels in rat intestines and monitoring subsequent tissue damage and regeneration. The combination of imaging techniques allowed for the determination of organ recovery prognosis based on blood supply. The system holds promise for improving real-time determination of blood supply and tissue prognosis during surgery.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2022)
Article
Computer Science, Interdisciplinary Applications
Woo June Choi, Jung-Ki Yoon, Bjorn Paulson, Chang-Hoon Lee, Jae-Joon Yim, Jong-Il Kim, Jun Ki Kim
Summary: The new video microscopy technique combines motion-contrast imaging and image correlation to provide objective and selective ciliary beat frequency (CBF) measurements. The method's performance was validated with in vitro human airway organoid models, showing robustness to optical focal shifts and exhibiting spatial heterogeneity and temperature dependence.
IEEE TRANSACTIONS ON MEDICAL IMAGING
(2022)
Article
Pediatrics
Jung-Man Namgoong, Shin Hwang, Gi-Young Ko, Hyunhee Kwon, Suhyeon Ha, Seak Hee Oh, Kyung Mo Kim
Summary: The study assessed the clinical usability of intraoperative cine-portogram in young pediatric patients undergoing LT for biliary atresia, demonstrating that this method is valuable for identifying and embolizing residual collateral veins in this patient population.
PEDIATRIC TRANSPLANTATION
(2022)
Article
Materials Science, Multidisciplinary
Youngkyu Kim, Minju Cho, Bjorn Paulson, Sung-Hoon Kim, Jun Ki Kim
Summary: This study demonstrates that administering dexmedetomidine to mice can reduce fluorescence fluctuations caused by respiration, improving the quality and resolution of intravital fluorescence imaging.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Chemistry, Analytical
Sanghwa Lee, Jeongmin Oh, Minju Cho, Jun Ki Kim
Summary: This study describes a minimally invasive imaging system capable of sensing even small tumors through a flexible fiber bundle microprobe, and demonstrates its use for the early diagnosis of bladder cancer in rats.
Article
Biochemistry & Molecular Biology
Mi Joung Kim, Seong Jun Lim, Youngmin Ko, Hye Eun Kwon, Joo Hee Jung, Hyunwook Kwon, Heounjeong Go, Yangsoon Park, Tae-Keun Kim, MinKyo Jung, Chan-Gi Pack, Young Hoon Kim, Kyunggon Kim, Sung Shin
Summary: This study aimed to identify and validate urinary exosomal proteins as biomarkers for antibody-mediated rejection (ABMR) after kidney transplantation. Through proteomic analysis, four specific proteins associated with ABMR were discovered. In the validation cohort, the levels of these proteins were significantly higher in the ABMR group compared to other groups. Immunohistochemical staining showed that these proteins were more abundant in the glomerulus of the ABMR group. The combined prediction probability of these urinary exosomal proteins was significantly correlated with the scores in the glomerulus and peritubular capillary, as well as the Banff g + ptc scores.
Article
Chemistry, Analytical
Bjorn Paulson, Saeed Bohlooli Darian, Youngkyu Kim, Jeongmin Oh, Marjan Ghasemi, Kwanhee Lee, Jun Ki Kim
Summary: Complex clinical and small-animal research procedures can benefit from dual-site imaging with the proposed endoscopic system, which enables multiple fluorescence microendoscopes to share light sources and optical relays on a single microscope base. The system achieves high channel isolation and submicron and subcellular resolution, as demonstrated by imaging experiments on USAF charts and dyed fiber tissues. Simultaneous liver and kidney imaging was also successfully demonstrated in mice. This multi-channel microendoscopy system provides a potentially low-cost means of simultaneous multiple endoscopic imaging, reducing animal harm and enhancing understanding of temporal connections between biological systems.
Article
Biotechnology & Applied Microbiology
Sanghwa Lee, Miyeon Jue, Minju Cho, Kwanhee Lee, Bjorn Paulson, Hanjoong Jo, Joon Seon Song, Soo-Jin Kang, Jun Ki Kim
Summary: This study proposes a method for diagnosing atherosclerosis in the carotid artery using nanometer biomarker measurements through surface-enhanced Raman spectroscopy (SERS) from single-drop blood samples. A diagnostic classifier was established based on principal component analysis (PCA), which separates the resultant spectra into the atherosclerotic and control groups. The accuracy of the PCA modification-based diagnostic criteria was 94.5%, and that of the machine learning algorithm 97.5%.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Chemistry, Analytical
Min Ju Cho, Chae-Eun Kim, Yeon Hui Shin, Jun Ki Kim, Chan-Gi Pack
Summary: Label-free optical diffraction tomography allows three-dimensional imaging of cells and organelles, providing valuable physical parameters such as refractive index and volume for quantitative analysis. The impact of various invasive cell manipulations, such as temperature change and chemical fixation, on cellular properties was investigated in this study. It was found that these manipulations can significantly alter the refractive index values of organelles, and the expression of GFP-tagged proteins had a larger effect compared to chemical fluorescence staining.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Marjan Ghasemi, Jeongmin Oh, Sunghoon Jeong, Mingyu Lee, Saeed Bohlooli Darian, Kyunghwan Oh, Jun Ki Kim
Summary: We have developed a fiber-optic sensor based on the Fabry-Perot interferometer principles for the detection of blood clot formation. The sensor consists of two types of sensor tips: a tapered fiber and an ultra-compact blood FPI on a single-mode fiber. The sensor's performance was evaluated by introducing varying quantities of thrombin reagent into the blood and analyzing the resulting clot-formation-indicating spectrum shifts.
Article
Chemistry, Analytical
Youngkyu Kim, Woo June Choi, Jeongmin Oh, Kwanhee Lee, Jun Ki Kim
Summary: Modern smartphones are being used in point-of-care devices due to their advanced form factor, computing, and display performances.With dual-wavelength laser speckle contrast imaging, a smartphone-combined handheld endoscope is developed to monitor blood flow changes and hemoglobin concentration in real-time. The combination of LSCI with smartphone endoscopy offers a valuable platform for understanding the functional hemodynamic changes in internal organs, which can benefit the diagnosis and treatment of vascular diseases in point-of-care testing.
Article
Surgery
Yu Jeong Cho, Hyunhee Kwon, Suhyeon Ha, Seong Chul Kim, Dae Yeon Kim, Jung -Man Namgoong, So Hyun Nam, Ju Yeon Lee, Eunyoung Jung, Min Jeng Cho
Summary: We analyzed the timing of inguinal hernia repair in premature infants in NICU and found that repairing the hernia after discharge may decrease the odds of recurrence and postoperative respiratory insufficiency. The significant factors affecting recurrence were preoperative ventilator dependence and body weight of <3,000 g at the time of surgery.
ANNALS OF SURGICAL TREATMENT AND RESEARCH
(2023)
Article
Pediatrics
Yong Jae Kwon, Dae Yeon Kim, Seong Chul Kim, Song Cheol Kim, Hyunhee Kwon, Jae Moon Choi, Jung-Man Namgoong
Summary: This study compared the perioperative outcomes of splenic vessel preservation (SVP) and the Warshaw technique (WT) in pediatric distal pancreatectomy. The results showed no significant differences in postoperative complications and the incidence of splenic infarctions or perigastric varices between the two techniques.
PEDIATRIC SURGERY INTERNATIONAL
(2022)