Article
Plant Sciences
Tanya A. Wagner, Aixing Gu, Sara E. Duke, Alois A. Bell, Clint Magill, Jinggao Liu
Summary: Research on Verticillium dahliae isolates from Xinjiang revealed different genotypes and subgenotypes associated with lineages 1A and 2B, corresponding to different pathotypes. Pathogenicity assays showed that isolates in lineage 1A caused greater leaf drop and shoot weight reduction compared to isolates in lineage 2B.
Article
Plant Sciences
Pablo Diaz-Rueda, Ana Aguado, Laura Romero-Cuadrado, Nieves Capote, Jose M. Colmenero-Flores
Summary: Resistance to the defoliating pathotype of Verticillium dahliae in olive was evaluated in 68 wild genotypes, with 15 genotypes showing resistance and the rest exhibiting variable levels of susceptibility. The study found high correlation between symptom severity and pathogen DNA levels in the stem of inoculated genotypes at 120 days after inoculation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Pedro Valverde, Carlos Trapero, Octavio Arquero, Nicolas Serrano, Diego Barranco, Concepcion Munoz Diez, Francisco J. Lopez-Escudero
Summary: This study found that grafting susceptible olive cultivars onto resistant rootstocks can delay the onset of disease symptoms, but after 4 years, it was observed that all combinations containing the susceptible cultivar Picual were extensively colonized by V.dahliae and developed severe symptoms of the disease, with plant mortality similar to Picual growing on its own roots. The research highlights the importance of long-term field experiments for evaluating Verticillium wilt of olive (VWO) and suggests that grafting susceptible olive cultivars onto resistant rootstocks may not provide a durable control of VWO under high inoculum potential.
Article
Plant Sciences
A. Serrano, D. Rodriguez-Jurado, B. Roman, J. Bejarano-Alcazar, R. De la Rosa, L. Leon
Summary: Genetic resistance is the most recommended measure to control verticillium wilt in olive, but additional evaluation under natural environmental conditions is necessary to confirm the resistance of breeding selections. Some selections showed potential resistance to Verticillium dahliae infection, while others had contradictory results compared to previous evaluations under controlled conditions, highlighting the need for long-term experimentation in natural environmental conditions.
Article
Plant Sciences
Nuria Montes-Osuna, Carmen Gomez-Lama Cabanas, Antonio Valverde-Corredor, Roeland L. Berendsen, Pilar Prieto, Jesus Mercado-Blanco
Summary: This study evaluated the potential involvement of selected phenotypes of the strain PICF7 in root colonization ability and VWO biocontrol. Results demonstrated that the phenotypes studied were irrelevant for VWO biocontrol.
Article
Horticulture
Sara Godena, Dario Ivic, Dean Ban, Smiljana Goreta Ban
Summary: Verticillium wilt, caused by Verticillium dahliae, is considered the most serious biotic threat to olive trees. This study in Istria, Croatia, identified the widespread presence of Verticillium wilt as well as the less aggressive nondefoliating pathotype and race 1 of V. dahliae for the first time in Croatia. Different susceptibilities of local olive cultivars to V. dahliae infection were observed, with Karbonaca scoring as the most resistant cultivar.
SCIENTIA HORTICULTURAE
(2022)
Article
Biology
Dan-Dan Zhang, Xiao-Feng Dai, Steven J. Klosterman, Krishna Subbarao, Jie-Yin Chen
Summary: This review systematically discusses the roles of the secretome of Verticillium dahliae in vascular occlusion and modulation of plant defence responses, highlighting its role in inducing Verticillium wilt. By manipulating plant defences and hormone levels, the secretome colludes with plant defence responses to modulate wilting symptoms, bridging historical concepts of both toxin production and vascular occlusion as the cause of Verticillium wilt.
BIOLOGICAL REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Aifang Ma, Dingpeng Zhang, Guangxing Wang, Kai Wang, Zhen Li, Yuanhui Gao, Hengchang Li, Chao Bian, Jinkui Cheng, Yinan Han, Shuhua Yang, Zhizhong Gong, Junsheng Qi
Summary: VDAL protein enhances plant resistance to Verticillium wilt by competing with MYB6 for binding to PUBs, leading to MYB6 ubiquitination and degradation. This study reveals the molecular mechanism of plants increasing disease resistance when overexpressing effector proteins without inducing a hypersensitive response.
Article
Plant Sciences
Manuel Anguita-Maeso, Jose Luis Trapero-Casas, Concepcion Olivares-Garcia, David Ruano-Rosa, Elena Palomo-Rios, Rafael M. Jimenez-Diaz, Juan A. Navas-Cortes, Blanca B. Landa
Summary: The research highlights that host resistance is the most practical, long-term, and economically efficient disease control measure for Verticillium wilt in olive caused by the xylem-invading fungus Verticillium dahliae. It also suggests that in vitro olive propagation may alter the diversity and composition of the xylem-inhabiting microbiome, potentially affecting the resistance response to the pathogen.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Ana Lopez-Moral, Carlos Agusti-Brisach, Francisco M. Leiva-Egea, Antonio Trapero
Summary: This study investigated the influence of olive genotype and biocontrol treatments on the effect of olive stem extract (OSE) on the viability of Verticillium dahliae conidia. The results showed that the resistance level of the cultivar affected the effectiveness of biocontrol treatments on the inhibition of conidial germination by OSE.
Article
Agronomy
Tianyi Wang, Muhammad Shaban, Junhui Shi, Weiran Wang, Shiming Liu, Xinhui Nie, Yu Yu, Jie Kong, Steven J. Klosterman, Xianlong Zhang, Alifu Aierxi, Longfu Zhu
Summary: The severity of Verticillium wilt on cotton caused by defoliating strains of Verticillium dahliae has gradually increased and threatens production worldwide. Identification of the molecular components of leaf defoliation may increase cotton tolerance to V. dahliae.
Article
Engineering, Environmental
Jing Zhang, Xinru Yu, Chaojun Zhang, Qiong Zhang, Ying Sun, Heqin Zhu, Canming Tang
Summary: The study showed that pectin lyase can enhance cotton resistance to Verticillium wilt and induce cell apoptosis of the V. dahliae strain Vd080. Pectin lyase affects various cellular processes in the Vd080 strain, leading to ER stress and ultimately cell apoptosis. These findings suggest that pectin lyase could be a potential biocontrol agent for managing Verticillium wilt in cotton.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Review
Plant Sciences
Yutao Zhu, Mei Zhao, Taotao Li, Lianzhe Wang, Chunli Liao, Dongxiao Liu, Huamin Zhang, Yanpeng Zhao, Lisen Liu, Xiaoyang Ge, Bingbing Li
Summary: Cotton is a widely grown crop due to its economic value. Verticillium wilt, caused by a pathogen called Verticillium dahliae, is a devastating disease that affects cotton crops, leading to yield losses and lower fiber quality. Developing genetically engineered cotton varieties with resistance to Verticillium wilt is an effective strategy, but there are challenges due to the lack of resistance gene resources. Understanding the molecular mechanisms of the interaction between V. dahliae and cotton is crucial in identifying genes related to disease resistance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Ana Lopez-Moral, Antonio Rafael Sanchez-Rodriguez, Antonio Trapero, Carlos Agusti-Brisach
Summary: The study aimed to establish a method for collecting root exudates from olive plants and evaluate their effects on the viability of Verticillium dahliae conidia and microsclerotia. The results showed that a 0.01 M CaSO4 solution and a collection period of 4 hours were the optimal combination for root exudate collection. The root exudates from 'Arbequina' and 'Picual' significantly induced the germination of conidia and microsclerotia, and the treatment with Aureobasidium pullulans AP08 enhanced the effectiveness of the root exudates in decreasing conidia and microsclerotia viability.
Article
Plant Sciences
Dan Wang, Jie-Yin Chen, Jian Song, Jun-Jiao Li, Steven J. Klosterman, Ran Li, Zhi-Qiang Kong, Krishna Subbarao, Xiao-Feng Dai, Dan-Dan Zhang
Summary: The V. dahliae genome encodes a family of six xylanases, with VdXyn4 being the only enzyme that degrades the plant cell wall and contributes to the virulence of the pathogen. VdXyn4 displays cytotoxic activity and induces necrosis in host plants during late infection stages, playing a crucial intracellular role. This cytotoxic activity is likely conserved in other enzyme families in plant vascular pathogens, suggesting its importance for pathogen colonization.
Article
Microbiology
Carmen Gomez-Lama Cabanas, Nuria M. Wentzien, Yasmin Zorrilla-Fontanesi, Antonio Valverde-Corredor, Antonio J. Fernandez-Gonzalez, Manuel Fernandez-Lopez, Jesus Mercado-Blanco
Summary: This study investigated the impact of the versatile biocontrol and plant-growth-promoting rhizobacteria Pseudomonas simiae PICF7 on the banana holobiont. Despite not significantly altering the composition or structure of the root microbiota, the presence of PICF7 led to a significant shift in microbial community interactions. The introduction of PICF7 did not result in major or permanent changes in the banana holobiont, indicating its potential as a beneficial rhizobacteria under field conditions.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Nuria Montes-Osuna, Tomislav Cernava, Carmen Gomez-Lama Cabanas, Gabriele Berg, Jesus Mercado-Blanco
Summary: This study assessed the antagonistic capacity of two endophytic bacteria against various plant pathogens and found that they produced a variety of volatile organic compounds (VOCs) with antifungal or plant growth promoting activities. The volatilomes of these bacteria differed significantly when co-incubated with Verticillium dahliae. Further research is needed to understand the modes of action and potential benefits of these VOCs for the host plant.
Article
Plant Sciences
Amedea B. Seabra, Neidiquele M. Silveira, Rafael Ribeiro, Joana C. Pieretti, Juan B. Barroso, Francisco J. Corpas, Jose M. Palma, John T. Hancock, Marek Petrivalsky, Kapuganti J. Gupta, David Wendehenne, Gary J. Loake, Jorg Durner, Christian Lindermayr, Arpad Molnar, Zsuzsanna Kolbert, Halley C. Oliveira
Summary: Nitric oxide (NO) is a versatile gas signal that regulates plant growth, development, and stress response. Nanotechnology offers a promising approach to enhance NO delivery to plants, as encapsulated NO-releasing nanomaterials have numerous advantages over non-encapsulated NO donors. These nano NO donors have proven effective in increasing tissue NO levels and enhancing NO effects in both animals and humans.
Article
Plant Sciences
Martina Cardoni, Carmen Gomez-Lama Cabanas, Antonio Valverde-Corredor, Rafael Villar, Jesus Mercado-Blanco
Summary: This study evaluated root functional traits, defense-related gene expression, root lignin content, and root membrane permeability in different olive varieties with varying tolerance/susceptibility to Verticillium wilt of olive (VWO). The results showed significant differences in root system architecture and lignin content between tolerant and susceptible cultivars, and tolerant cultivars exhibited a stronger and more rapid defense response to the pathogen.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Lorena Aranda-Cano, Raquel Valderrama, Jose Rafael Pedrajas, Juan C. Begara-Morales, Mounira Chaki, Maria N. Padilla, Manuel Melguizo, Francisco Javier Lopez-Jaramillo, Juan B. Barroso
Summary: Heat stress leads to oxidative stress and heat shock response in yeast cells. Nitro-fatty acids act as signaling molecules through nitroalkylation, and their presence in Saccharomyces cerevisiae is identified for the first time. Nitroalkylation of cytosolic peroxiredoxin Tsa1 affects its activity and amino acid targets. This response mechanism plays a key role in regulating defense mechanisms against oxidative stress in yeast, and is faster than gene induction and protein synthesis.
Article
Microbiology
Maria Isabella Prigigallo, Carmen Gomez-Lama Cabanas, Jesus Mercado-Blanco, Giovanni Bubici
Summary: By designing a synthetic microbial community (SynCom), the spread of Fusarium wilt of banana (FWB) can be effectively controlled. Microbes in the community not only antagonize the pathogen but also interact with each other. The accurate design of SynCom can improve biocontrol results, which is of great importance for the biological control of FWB.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Agronomy
Maria de la O. Leyva-Perez, Lea Vexler, Stephen Byrne, Corentin R. Clot, Fergus Meade, Denis Griffin, Tom Ruttink, Jie Kang, Dan Milbourne
Summary: We have developed a novel low-cost genome-scanning marker platform called PotatoMASH. By using multiplex amplicon sequencing, PotatoMASH efficiently analyzes 339 multi-allelic regions in the potato genome and has been successfully applied to over 700 potato lines. PotatoMASH is able to diagnose pest-resistance markers, detect quantitative trait loci, and track genetic variation.
Article
Plant Sciences
Martina Cardoni, Lucia Olmo-Garcia, Irene Serrano-Garcia, Alegria Carrasco-Pancorbo, Jesus Mercado-Blanco
Summary: Verticillium wilt of olive (VWO), caused by Verticillium dahliae, is a major concern in many olive-growing countries. This study found a strong relationship between the quantitative basal composition of the root secondary metabolic profile and VWO tolerance/susceptibility of olive varieties. Tolerant cultivars showed higher content of secoiridoids, while the susceptible ones presented greater amounts of verbascoside and methoxypinoresinol glucoside.
JOURNAL OF PLANT INTERACTIONS
(2023)
Article
Genetics & Heredity
Martina Cardoni, Antonio J. Fernandez-Gonzalez, Antonio Valverde-Corredor, Manuel Fernandez-Lopez, Jesus Mercado-Blanco
Summary: This study examined the effects of two biological control agents (BCAs) on the structure, composition, and co-occurrence networks of olive root-associated microbial communities. The BCAs did not significantly change the structure or composition of the microbiota, but they did alter the interactions within the networks. These findings have important practical implications for the future field applications of BCAs.
ENVIRONMENTAL MICROBIOME
(2023)
Review
Plant Sciences
Martina Cardoni, Jesus Mercado-Blanco
Summary: The holobiont concept has greatly advanced our understanding of plant-associated microbiomes and their importance for plant development, growth, and resilience. This comprehensive review consolidates the existing knowledge of stress factors affecting olive cultivation and the microbiota associated with olive tissues and organs. The review emphasizes the need for a holistic approach and the consideration of the holobiont conceptual framework in future research to enhance olive health and productivity.
FRONTIERS IN PLANT SCIENCE
(2023)
Editorial Material
Plant Sciences
Iakovos S. Pantelides, Ioannis A. Stringlis, Omri M. Finkel, Jesus Mercado-Blanco
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Nuria M. Wentzien, Antonio J. Fernandez-Gonzalez, Pablo J. Villadas, Antonio Valverde-Corredor, Jesus Mercado-Blanco, Manuel Fernandez-Lopez
Summary: Soil health and root-associated microbiome are intertwined factors affecting plant health. The addition of manure to agricultural fields enhances soil nutrient content and water retention. This study investigated the impact of ovine manure on the microbial communities in the rhizosphere and root endosphere of olive trees. Results showed that manure amendment significantly influenced the microbial community, increasing fungal richness, diversity, and biomass in the rhizosphere. Soil nutrient content was also improved in the manure-amended orchard, and the microbial cooccurrence network displayed more positive interactions and complexity. Overall, manure amendment is an effective tool for modulating the plant-associated microbial community.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Correction
Biochemistry & Molecular Biology
Antonio J. Fernandez-Gonzalez, Jorge A. Ramirez-Tejero, Maria Patricia Nevado-Berzosa, Francisco Luque, Manuel Fernandez-Lopez, Jesus Mercado-Blanco
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)