Agrobacterium-Mediated Transformation of Tree Fruit Crops: Methods, Progress, and Challenges

The Cold-Regulated Genes of Blueberry and Their Response to Overexpression of VcDDF1 in Several Tissues

(2018) Aaron Walworth et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Mitigation of global warming impact of fresh fruit production through climate smart management

(2018) Angela Fiore et al.   JOURNAL OF CLEANER PRODUCTION

Adventitious shoot regeneration and Agrobacterium tumefaciens-mediated transformation of leaf explants of sweet cherry (Prunus avium L.)

(2018) Xiaojuan Zong et al.   JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY

Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins

(2018) Zhen Liang et al.   Nature Protocols

Optimized paired-sgRNA/Cas9 cloning and expression cassette triggers high-efficiency multiplex genome editing in kiwifruit

(2018) Zupeng Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

A versatile and robust Agrobacterium-based gene stacking system generates high-quality transgenic Arabidopsis plants

(2018) Ray Collier et al.   PLANT JOURNAL

Selective Targeting of Mobile mRNAs to Plasmodesmata for Cell-to-Cell Movement

(2018) Kai-Ren Luo et al.   PLANT PHYSIOLOGY

The Major Origin of Seedless Grapes Is Associated with a Missense Mutation in the MADS-Box GeneVviAGL11

(2018) Carolina Royo et al.   PLANT PHYSIOLOGY

Embryo-specific expression of a visual reporter gene as a selection system for citrus transformation

(2018) Manjul Dutt et al.   PLoS One

Current achievements and future directions in genetic engineering of European plum (Prunus domestica L.)

(2018) Cesar Petri et al.   TRANSGENIC RESEARCH

Transgenic American Chestnuts Do Not Inhibit Germination of Native Seeds or Colonization of Mycorrhizal Fungi

(2018) Andrew E. Newhouse et al.   Frontiers in Plant Science

A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

(2018) Longzheng Chen et al.   Horticulture Research

Overexpression of the MADS-box gene K-domain increases the yield potential of blueberry

(2018) Guo-qing Song et al.   PLANT SCIENCE

Three FT and multiple CEN and BFT genes regulate maturity, flowering, and vegetative phenology in kiwifruit

(2017) Charlotte Voogd et al.   JOURNAL OF EXPERIMENTAL BOTANY

Adjusting the scent ratio: using genetically modified Vitis vinifera plants to manipulate European grapevine moth behaviour

(2017) Umberto Salvagnin et al.   PLANT BIOTECHNOLOGY JOURNAL

Nanobody-mediated resistance to Grapevine fanleaf virus in plants

(2017) Caroline Hemmer et al.   PLANT BIOTECHNOLOGY JOURNAL

CRISPR/Cas9-mediated efficient targeted mutagenesis in grape in the first generation

(2017) Xianhang Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering canker-resistant plants through CRISPR/Cas9-targeted editing of the susceptibility gene CsLOB1 promoter in citrus

(2017) Aihong Peng et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome editing of the disease susceptibility gene CsLOB1 in citrus confers resistance to citrus canker

(2017) Hongge Jia et al.   PLANT BIOTECHNOLOGY JOURNAL

In vitro gene expression and mRNA translocation from transformed walnut (Juglans regia) rootstocks expressing DsRED fluorescent protein to wild-type scions

(2017) Xiaochen Liu et al.   PLANT CELL REPORTS

Rapid and efficient CRISPR/Cas9 gene editing in Citrus using the YAO promoter

(2017) Fei Zhang et al.   PLANT CELL REPORTS

Construction of high efficiency regeneration and transformation systems of Pyrus ussuriensis Maxim

(2017) Yingjie Yang et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Overexpression ofMsDREB6.2results in cytokinin-deficient developmental phenotypes and enhances drought tolerance in transgenic apple plants

(2017) Xiong Liao et al.   PLANT JOURNAL

High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9

(2017) Javier Gil-Humanes et al.   PLANT JOURNAL

A simple and efficient in planta transformation method for pommelo ( Citrus maxima ) using Agrobacterium tumefaciens

(2017) Yong-yan Zhang et al.   SCIENTIA HORTICULTURAE

Transgenic Cavendish bananas with resistance to Fusarium wilt tropical race 4

(2017) James Dale et al.   Nature Communications

Transgenic citrus plants expressing the p35 anti-apoptotic gene have altered response to abiotic stress

(2017) Vladimir Orbović et al.   Horticulture Environment and Biotechnology

Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion

(2017) Carlos Pulido-Quetglas et al.   PLoS Computational Biology

Gene Editing and Crop Improvement Using CRISPR-Cas9 System

(2017) Leena Arora et al.   Frontiers in Plant Science

Grapevine Grafting: Scion Transcript Profiling and Defense-Related Metabolites Induced by Rootstocks

(2017) Walter Chitarra et al.   Frontiers in Plant Science

Editing Citrus Genome via SaCas9/sgRNA System

(2017) Hongge Jia et al.   Frontiers in Plant Science

New Biotechnological Tools for the Genetic Improvement of Major Woody Fruit Species

(2017) Cecilia Limera et al.   Frontiers in Plant Science

CRISPR/Cas9-mediated targeted mutagenesis in grape

(2017) Ikuko Nakajima et al.   PLoS One

Dual regulation of water retention and cell growth by a stress-associated protein (SAP) gene in Prunus

(2017) Alba Lloret et al.   Scientific Reports

Identification of genomic sites for CRISPR/Cas9-based genome editing in the Vitis vinifera genome

(2016) Yi Wang et al.   BMC PLANT BIOLOGY

Synthesis of an artificial Vitis vinifera miRNA 319e using overlapping long primers and its application for gene silencing

(2016) Álvaro Castro et al.   JOURNAL OF BIOTECHNOLOGY

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

(2016) Ines Fonfara et al.   NATURE

Homologous and heterologous expression of grapevine E-(β)-caryophyllene synthase (VvGwECar2)

(2016) Umberto Salvagnin et al.   PHYTOCHEMISTRY

Overexpression ofMdbHLH104gene enhances the tolerance to iron deficiency in apple

(2016) Qiang Zhao et al.   PLANT BIOTECHNOLOGY JOURNAL

The enhancement of tolerance to salt and cold stresses by modifying the redox state and salicylic acid content via thecytosolic malate dehydrogenasegene in transgenic apple plants

(2016) Qing-Jie Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Precocious flowering of juvenile citrus induced by a viral vector based onCitrus leaf blotch virus: a new tool for genetics and breeding

(2016) Karelia Velázquez et al.   PLANT BIOTECHNOLOGY JOURNAL

The knock-down of the expression ofMdMLO19reduces susceptibility to powdery mildew (Podosphaera leucotricha) in apple (Malus domestica)

(2016) Stefano Pessina et al.   PLANT BIOTECHNOLOGY JOURNAL

Agrobacterium-mediated transformation of avocado (Persea americana Mill.) somatic embryos with fluorescent marker genes and optimization of transgenic plant recovery

(2016) Elena Palomo-Ríos et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Kn1 gene overexpression drastically improves genetic transformation efficiencies of citrus cultivars

(2016) Wei Hu et al.   PLANT CELL TISSUE AND ORGAN CULTURE

An optimized TRV-based virus-induced gene silencing protocol for Malus crabapple

(2016) Jie Zhang et al.   PLANT CELL TISSUE AND ORGAN CULTURE

A non-antibiotic selection strategy uses the phosphomannose-isomerase (PMI) gene and green fluorescent protein (GFP) gene for Agrobacterium-mediated transformation of Prunus domestica L. leaf explants

(2016) Tatiana Sidorova et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Transgenic apple plants overexpressing the chalcone 3-hydroxylase gene of Cosmos sulphureus show increased levels of 3-hydroxyphloridzin and reduced susceptibility to apple scab and fire blight

(2016) Olly Sanny Hutabarat et al.   PLANTA

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

(2016) Sergei Svitashev et al.   Nature Communications

Grafting: A Technique to Modify Ion Accumulation in Horticultural Crops

(2016) Muhammad A. Nawaz et al.   Frontiers in Plant Science

Overexpression of a Modified Plant Thionin Enhances Disease Resistance to Citrus Canker and Huanglongbing (HLB)

(2016) Guixia Hao et al.   Frontiers in Plant Science

DNA-Free Genetically Edited Grapevine and Apple Protoplast Using CRISPR/Cas9 Ribonucleoproteins

(2016) Mickael Malnoy et al.   Frontiers in Plant Science

An apple rootstock overexpressing a peach CBF gene alters growth and flowering in the scion but does not impact cold hardiness or dormancy

(2016) Timothy S Artlip et al.   Horticulture Research

Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine

(2016) Stefano Pessina et al.   Horticulture Research

CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)

(2016) Chong Ren et al.   Scientific Reports

Efficient Genome Editing in Apple Using a CRISPR/Cas9 system

(2016) Chikako Nishitani et al.   Scientific Reports

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Production of transgenic kiwifruit plants harboring the SbtCry1Ac gene

(2015) H.Y. Zhang et al.   GENETICS AND MOLECULAR RESEARCH

Alternative CRISPR system could improve genome editing

(2015) Heidi Ledford   NATURE

Nonbrowning GM apple cleared for market

(2015) Emily Waltz   NATURE BIOTECHNOLOGY

Evaluation of a grapevine-derived reporter gene system for precision breeding of Vitis

(2015) R. Kandel et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Establishment of a picloram-induced somatic embryogenesis system in Vitis vinifera cv. chardonnay and genetic transformation of a stilbene synthase gene from wild-growing Vitis species

(2015) Lingmin Dai et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Efficient heat-shock removal of the selectable marker gene in genetically modified grapevine

(2015) Lorenza Dalla Costa et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Development of Agrobacterium-mediated transient transformation in persimmon (Diospyros kaki Thunb.)

(2015) Rongli Mo et al.   SCIENTIA HORTICULTURAE

Agrobacterium: nature’s genetic engineer

(2015) Eugene W. Nester   Frontiers in Plant Science

Overexpression of a peach CBF gene in apple: a model for understanding the integration of growth, dormancy, and cold hardiness in woody plants

(2015) Michael Wisniewski et al.   Frontiers in Plant Science

Cisgenic apple trees; development, characterization, and performance

(2015) Frans A. Krens et al.   Frontiers in Plant Science

Development of the First Cisgenic Apple with Increased Resistance to Fire Blight

(2015) Thomas D. Kost et al.   PLoS One

Improvement of Agrobacterium-mediated transformation and rooting of black cherry

(2014) Ying Wang et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Overexpression of the kiwifruit SVP3 gene affects reproductive development and suppresses anthocyanin biosynthesis in petals, but has no effect on vegetative growth, dormancy, or flowering time

(2014) Rongmei Wu et al.   JOURNAL OF EXPERIMENTAL BOTANY

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

Rootstock-to-scion transfer of transgene-derived small interfering RNAs and their effect on virus resistance in nontransgenic sweet cherry

(2014) Dongyan Zhao et al.   PLANT BIOTECHNOLOGY JOURNAL

DNA Replicons for Plant Genome Engineering

(2014) N. J. Baltes et al.   PLANT CELL

Ethylene inhibitor silver nitrate enhances regeneration and genetic transformation of Prunus avium (L.) cv Stella

(2014) Tiziana Sgamma et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Transgenic American chestnuts show enhanced blight resistance and transmit the trait to T1 progeny

(2014) Andrew E. Newhouse et al.   PLANT SCIENCE

RNA interference: concept to reality in crop improvement

(2014) Satyajit Saurabh et al.   PLANTA

Targeted Genome Editing of Sweet Orange Using Cas9/sgRNA

(2014) Hongge Jia et al.   PLoS One

Progress of cereal transformation technology mediated by Agrobacterium tumefaciens

(2014) Yukoh Hiei et al.   Frontiers in Plant Science

Plant–Agrobacterium interaction mediated by ethylene and super-Agrobacterium conferring efficient gene transfer

(2014) Satoko Nonaka et al.   Frontiers in Plant Science

Development of an efficient regeneration and Agrobacterium-mediated transformation system in crab apple (Malus micromalus) using cotyledons as explants

(2013) Hongyan Dai et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

A dsRNA-binding protein MdDRB1 associated with miRNA biogenesis modifies adventitious rooting and tree architecture in apple

(2013) Chun-Xiang You et al.   PLANT BIOTECHNOLOGY JOURNAL

Reduced generation time of apple seedlings to within a year by means of a plant virus vector: a new plant-breeding technique with no transmission of genetic modification to the next generation

(2013) Noriko Yamagishi et al.   PLANT BIOTECHNOLOGY JOURNAL

Intragenesis and cisgenesis as alternatives to transgenic crop development

(2013) Inger Baeksted Holme et al.   PLANT BIOTECHNOLOGY JOURNAL

Engineering cherry rootstocks with resistance to Prunus necrotic ring spot virus through RNAi-mediated silencing

(2013) Guo-qing Song et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection

(2013) Xiuping Zou et al.   PLANT CELL REPORTS

Recent advances in genetic engineering for improvement of fruit crops

(2013) Manoj K. Rai et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Genetic engineering of Plum pox virus resistance: ‘HoneySweet’ plum—from concept to product

(2013) Ralph Scorza et al.   PLANT CELL TISSUE AND ORGAN CULTURE

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Rootstock-regulated gene expression patterns associated with fire blight resistance in apple

(2012) Philip J Jensen et al.   BMC GENOMICS

A chemical-inducible Cre-LoxP system allows for elimination of selection marker genes in transgenic apricot

(2012) César Petri et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Plum (Prunus domestica) Trees Transformed with Poplar FT1 Result in Altered Architecture, Dormancy Requirement, and Continuous Flowering

(2012) Chinnathambi Srinivasan et al.   PLoS One

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Mobility of Transgenic Nucleic Acids and Proteins within Grafted Rootstocks for Agricultural Improvement

(2012) Victor M. Haroldsen et al.   Frontiers in Plant Science

Traversing the Cell: Agrobacterium T-DNA’s Journey to the Host Genome

(2012) Stanton B. Gelvin   Frontiers in Plant Science

Ectopic expression of class 1 KNOX genes induce adventitious shoot regeneration and alter growth and development of tobacco (Nicotiana tabacum L) and European plum (Prunus domestica L)

(2011) C. Srinivasan et al.   PLANT CELL REPORTS

Phylogeography and molecular epidemiology of Papaya ringspot virus

(2011) X.A. Olarte Castillo et al.   VIRUS RESEARCH

Current Epidemiological Understanding of Citrus Huanglongbing

(2010) Tim R. Gottwald   Annual Review of Phytopathology

Precocious flowering in trees: the FLOWERING LOCUS T gene as a research and breeding tool in Populus

(2010) Huanling Zhang et al.   JOURNAL OF EXPERIMENTAL BOTANY

Adventitious shoot regeneration from leaf explants of southern highbush blueberry cultivars

(2010) Cheng Liu et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Effects of transgenic rootstocks on growth and development of non-transgenic scion cultivars in apple

(2010) Anders Smolka et al.   TRANSGENIC RESEARCH

High frequency of single-copy T-DNA transformants produced by floral dip inCRE-expressing Arabidopsis plants

(2009) Annelies De Paepe et al.   PLANT JOURNAL