Collateral damage and CRISPR genome editing

Long reads: their purpose and place

(2018) Martin O Pollard et al.   HUMAN MOLECULAR GENETICS

Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos

(2018) Bin Gu et al.   NATURE BIOTECHNOLOGY

CRISPR off-target analysis in genetically engineered rats and mice

(2018) Keith R. Anderson et al.   NATURE METHODS

No unexpected CRISPR-Cas9 off-target activity revealed by trio sequencing of gene-edited mice

(2018) Vivek Iyer et al.   PLoS Genetics

Genetic variation may confound analysis of CRISPR-Cas9 off-target mutations

(2018) Guanqun Wang et al.   Cell Discovery

Large deletions induced by Cas9 cleavage

(2018) Fatwa Adikusuma et al.   NATURE

In vivo CRISPR editing with no detectable genome-wide off-target mutations

(2018) Pinar Akcakaya et al.   NATURE

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Erratum: Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

(2018) Michael Kosicki et al.   NATURE BIOTECHNOLOGY

Mutation frequency is not increased in CRISPR–Cas9-edited mice

(2018) Michaela Willi et al.   NATURE METHODS

Dual-spindle formation in zygotes keeps parental genomes apart in early mammalian embryos

(2018) Judith Reichmann et al.   SCIENCE

Directed evolution of CRISPR-Cas9 to increase its specificity

(2018) Jungjoon K. Lee et al.   Nature Communications

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

Unexpected mutations after CRISPR–Cas9 editing in vivo

(2017) Kellie A Schaefer et al.   NATURE METHODS

CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets

(2017) Shengdar Q Tsai et al.   NATURE METHODS

CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome

(2017) Ha Youn Shin et al.   Nature Communications

Revealing hidden complexities of genomic rearrangements generated with Cas9

(2017) Katharina Boroviak et al.   Scientific Reports

Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing

(2016) Angelo Amabile et al.   CELL

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

(2014) Y. Lin et al.   NUCLEIC ACIDS RESEARCH

Quantifying Genome-Editing Outcomes at Endogenous Loci with SMRT Sequencing

(2014) Ayal Hendel et al.   Cell Reports

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

(2014) Steven Lin et al.   eLife

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY