Article
Chemistry, Analytical
Zi-Jian Chen, Zhicheng Huang, Song Huang, Jin-Lin Zhao, Yuanming Sun, Zhen-Lin Xu, Juewen Liu
Summary: This study explores the application of CeO2 nanoparticles in immunoassays and their adsorption behavior towards proteins, demonstrating the potential of CeO2 in bioanalytical chemistry, especially in terms of stability and tolerance to extreme conditions.
Article
Chemistry, Physical
Yuwei Li, Li Liu, Hanying Zhao
Summary: By utilizing hydrophobic interaction, multienzyme proteinosomes are fabricated with p-gal, GOx, and HRP on the surfaces in a model system using PDEGMA and bovine serum albumin. The immobilized enzymes show higher bioactivities than the free enzymes, facilitating direct transfer of the product as a substrate from one enzyme molecule to another.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Ping Wen, Xueyi Wang, Silvia Moreno, Susanne Boye, Dagmar Voigt, Brigitte Voit, Xin Huang, Dietmar Appelhans
Summary: The study presents an efficient way to construct a hierarchical multicompartment composed of hydrogel-filled proteinosome hybrid structure with polymersomes for substance transport. The pH-responsive behavior of the system allows for reversible swelling-deswelling and on-demand cargo release in response to enzymatic reactions, fulfilling the requirements of eukaryotic cell biomimetics.
Article
Engineering, Environmental
Yaqing Liu, Juan Fu, Langping Wu, Steffen Kuemmel, Ivonne Nijenhuis, Hans H. Richnow
Summary: In this study, multi-element compound-specific stable isotope analysis was used to evaluate the α-HCH and γ-HCH transformations catalyzed by LinA1 and LinA2 enzymes. The results showed slight differences in the isotopic fractionation patterns of different enantiomers, but both enzymes exhibited similar reaction mechanisms during γ-HCH transformation.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Qing Liu, Shuo Yang, Iris Seitz, Anna-Maria Makri Pistikou, Tom F. A. de Greef, Mauri A. Kostiainen
Summary: This study demonstrates the effective removal of heparin using a protein-based microcompartment. The proteinosome can selectively capture heparin and has advantages over serum albumin in this process. Moreover, the cytotoxicity of the scavenger is reduced by protocell shielding.
Article
Biochemistry & Molecular Biology
Mengfei Gao, Dishi Wang, Michaela Wilsch-Braeuninger, Weihua Leng, Jonathan Schulte, Nina Morgner, Dietmar Appelhans, T-Y. Dora Tang
Summary: This article presents a novel approach to generate proteinosomes that can support cell free transcription and translation. The study demonstrates the stability and enhanced protein expression of native proteinosomes under high salt conditions. It also shows the integration of native proteinosomes into higher order synthetic cellular architectures.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Chemistry, Applied
Xianfeng Lin, Qianru Yu, Wei Yang, Chuxian He, You Zhou, Nuo Duan, Shijia Wu
Summary: A new double-enzymes-modulated fluorescent assay based on the quenching of upconversion nanoparticles by Fe3+ was developed for sensitive determination of organophosphorus pesticides. The method showed rapidity, sensitivity, selectivity, stability, ease of operation, and strong anti-interference capability, making it promising for applications in food safety.
Review
Biotechnology & Applied Microbiology
Clare Mills, Katrina Campbell
Summary: There is an increasing demand for rapid and affordable in-field screening methods for low molecular weight (LMW) compound detection. This review examines the recent resurgence of using anti-idiotypes (Ab2s) and the potential role of phage display and nanobodies in improving LMW compound immunoassays.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xin Qiao, Xiaoliang Wang, Haixu Chen, Yan Huang, Shangsong Li, Luxuan Li, Yinyong Sun, Xiaoman Liu, Xin Huang
Summary: Modulated membrane functionalization is crucial for the application of hollow microcompartments. This study demonstrates the generation of phospholipid hybrid proteinosomes, which possess the combined advantages of liposomes and proteinosomes. The incorporation of phospholipids improves membrane fluidity and permeability, enabling stepwise release of encapsulated compounds and cascaded enzymatic reactions. These phospholipid hybrid proteinosomes can serve as an improved microcompartmental model for advanced artificial cell design and microreactor applications.
Article
Chemistry, Multidisciplinary
Patrick J. Grimes, Mary Jenkinson-Finch, Henry E. Symons, Wuge H. Briscoe, Sebastien Rochat, Stephen Mann, Pierangelo Gobbo
Summary: The synthesis of a novel photocleavable, poly(ethylene) glycol-based crosslinker enables the realization of light-induced disassembly, pattern generation and membrane permeability modulation in protocells, opening up new avenues for the engineering of organized protocell networks and micro-compartmentalized systems for information storage and release.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Harindu Rajapaksha, Dinesh R. Pandithavidana, Jayangika N. Dahanayake
Summary: Chronic kidney disease of unknown etiology (CKDu) is recognized as a global non-communicable health issue related to pesticides. This study used computational methods to analyze the effects of pesticide metabolites on CKDu, revealing that some metabolites may have higher binding interactions with renal enzymes compared to parent pesticides.
Article
Chemistry, Multidisciplinary
Vanessa Leal Pires, Sara C. Novais, Marco F. L. Lemos, Vanessa F. Fonseca, Bernardo Duarte
Summary: The study evaluated three indexes for their sensitivity in revealing toxically induced stress patterns in cells of the diatom Phaeodactylum tricornutum under contaminant exposure, with the integrated biomarker response index (IBR) excelling for statistically significant dose-response patterns for four out of the five tested compounds.
APPLIED SCIENCES-BASEL
(2021)
Review
Microbiology
Saleem Jaffar, Sajjad Ahmad, Yongyue Lu
Summary: Synthetic pesticides are extensively used worldwide to control pests, but their toxic residues have caused severe threats to non-target organisms. Biological methods, especially employing microbial species and their enzymes, are considered more efficient and eco-friendly for the complete removal of toxic compounds. The insect gut microbiota plays a crucial role in degrading toxic compounds and developing resistance mechanisms against pesticides.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Musarrat Ramzan, Mehvish Akram, Ashfaq Ahmad Rahi, Muhammad Mubashir, Liaqat Ali, Shah Fahad, Jiri Krucky, Sami Al Obaid, Mohammad Javed Ansari, Rahul Datta
Summary: The study compared the effects of pesticides on sunflower and mustard plants at different concentrations. Increasing cypermethrin concentration led to decreased fresh and dry weight, decreased root length, and increased shoot length. Pesticide also increased oxidative stress and altered anatomical structures in plant tissues.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Environmental Sciences
Leici Maria Machado Reichert, Diogo Reis de Oliveira, Jonas Londero Papaleo, Artur Antunes Navarro Valgas, Guendalina Turcato Oliveira
Summary: Pesticide usage has raised concerns about its impact on non-target organisms, particularly amphibians. In this study, tadpoles of Rhinella icterica were collected from a pesticide-free site and exposed to three herbicides and their mixtures. Although the mortality rate was low, significant variations in oxidative balance and body condition were observed among the experimental groups. The mixture of the herbicides was found to be more harmful, highlighting the need for stricter regulations on the use of mixed herbicides.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)