Article
Chemistry, Analytical
Dongwei Wang, Jiasheng Wang
Summary: The study demonstrated the potential of functionalized polypyrrole nanowires as a platform for label-free miRNA detection using EIS, and constructed a sensitive and selective miRNA biosensor based on PPyNWs/PAMAM hybrid. The biosensor showed excellent sensing performances in monitoring DNA/miRNA hybridization, indicating the microstructure of the PPy nanowires array greatly improved its performance.
Article
Chemistry, Analytical
Ziheng Hu, Ruifeng Zhu, Gabriela Figueroa-Miranda, Lei Zhou, Lingyan Feng, Andreas Offenhaeusser, Dirk Mayer
Summary: This study presents an electrochemical aptasensor for highly sensitive and selective serotonin (ST) detection, based on truncated DNA aptamers and a polyethylene glycol (PEG) molecule-functionalized sensing interface. The aptasensor shows improved sensitivity and enhanced antifouling ability due to the small size and stable structure of the truncated aptamers, as well as the blocking effect of PEG molecules. The sensor exhibits a wide detection range and low limit of detection, and also demonstrates high selectivity and stability in detecting ST in human serum and artificial cerebrospinal fluid (aCSF).
Article
Polymer Science
Shang-Lin Yeh, Piyush Deval, Wei-Bor Tsai
Summary: In this study, a simple and universal technique using pyrogallol-assisted immobilization of poly(ethylene glycol) (PEG) was introduced to deposit a thin antifouling layer on biomaterials. By controlling the amounts of PG and PEG and the coating time, the PG/PEG coating was able to reduce more than 99% of cell adhesion and protein adsorption, while providing a transparent and robust coating that can survive sterilization conditions. This technique has great potential for applications in transparent antifouling coatings for biomedical devices.
Article
Biochemical Research Methods
Dongwei Wang, Jiasheng Wang, Zhiling Song, Ni Hui
Summary: Ultrasensitive and low-fouling microRNA electrochemical biosensors were constructed by introducing thiol-terminated antifouling molecules onto polyaniline-modified electrodes. Among the investigated antifouling materials, a newly designed and synthesized peptide showed superior antifouling ability and highest specificity for various microRNA targets. The introduction of antifouling molecules did not compromise the sensitivity of the biosensor, indicating promising applications in developing biofouling-resistant sensors and biosensors.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yang Li, Rui Han, Min Chen, Leyao Zhang, Guixiang Wang, Xiliang Luo
Summary: An antifouling biosensing interface was constructed using cross-linked BSA and polyaniline nanowires, which exhibited enhanced antifouling capability and electrochemical activity. By immobilizing peptide aptamer for IgG recognition, an electrochemical biosensor with excellent selectivity and sensitivity was prepared, capable of accurately detecting IgG in complex biological samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yong-ping Gao, Ke-jing Huang, Fu-ting Wang, Yang-yang Hou, Lu-di Zhao, Bo-ya Wang, Jing Xu, Honglei Shuai, Guoqiang Li
Summary: A self-powered biosensor (SPB) utilizing a capacitor/enzymatic biofuel cell (EBFC), rolling circle amplification (RCA), and a digital multimeter (DMM) was developed for ultra-sensitive detection of miR-155. The EBFC-SPB exhibited a sensitivity of 15.85 mu A/pM with the matching capacitor, which is 513% higher than without capacitor (3.09 mu A/pM), achieving the first signal amplification. RCA triggered by miR-155 generated continuous electrons that flowed to the biocathode, catalyzing oxygen reduction and achieving the second signal amplification. DMM was used for signal conversion and real-time amplification, resulting in a LOD of 0.17 fM and a linear range of 0.5 fM to 10,000 fM, indicating the broad application prospects of EBFC-SPB for ultra-sensitive detection of miR-155.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biophysics
Liang Tian, Jiyuan Zhang, Yi Zhang, Olayinka Oderinde, Caiyun Li, Lili Duan, Yihong Wang, Jiansheng Cui
Summary: A versatile ratiometric electrochemical biosensing system was constructed for the detection of miRNAs, using a combination of target triggered bipedal DNAzyme walker cleavage cycling amplification and planar intercalated methylene blue molecules amplification. The system showed high sensitivity and specificity, with the ability to distinguish different miRNA sequences, making it promising for clinical application in early cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Eleni Koukouviti, Christos Kokkinos
Summary: The study presents a novel miniature all-3D-printed 4-electrode biochip capable of simultaneous determination of different biomarkers, showcasing high sensitivity and transferability.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Chen Wang, Yujie Liu, Ruoping Chen, Xiaoqiang Wang, Yunkun Wang, Jia Wei, Kun Zhang, Chenran Zhang
Summary: This study reports an electrochemical biosensor for the highly sensitive label-free detection of CSF miR-21, which offers high specificity and good performance for the quantification of miR-21 in clinical CSF samples and for accurate discrimination of medulloblastoma against non-cancer controls.
Article
Chemistry, Analytical
Decheng Zhang, Xiudan Lang, Ni Hui, Jiasheng Wang
Summary: The inappropriate and excessive use of pesticides poses a great challenge to human health. This study proposes a dual-mode biosensor for ultrafast and ultratrace detection of acetamiprid (ACE). The biosensor exhibits high sensitivity and speed, and has been successfully applied to detect ACE residues in complex soil samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Physical
Wenshuai Yang, Ziqian Zhao, Mingfei Pan, Lu Gong, Feiyi Wua, Charley Huang, Xiaogang Wang, Jianmei Wang, Hongbo Zeng
Summary: This study hypothesized that PEG polymer can be robustly attached onto substrates with the assistance of a bio-glue layer, and proposed a coating strategy for fabricating stable coatings with outstanding antifouling properties in various environmental engineering applications. Experimental results showed that the PEGDE/LP coating exhibited excellent stability and superior antifouling properties, suitable for separating oil-in-water emulsions.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Ning Gao, Xueying Wang, Jinhui Feng, Xiaojian Li, Huan Wang, Dawei Fan, Yong Zhang, Hongmin Ma, Qin Wei, Huangxian Ju
Summary: A photoelectrochemical biosensing interface was developed for detecting cancer cells using nano-structured platforms coupled with surface engineering. The biosensor exhibited a wide linear range for detecting breast cancer cells and successfully differentiated the expression of folate receptors on various cancer cells, showing great potential for cancer cell detection.
ADVANCED MATERIALS INTERFACES
(2021)
Article
Nanoscience & Nanotechnology
Marc Zinggeler, Sandra Schaer, Felix Kurth
Summary: This study describes a novel polymer/carbon nanotube composite coating that can be produced using fast and scalable printing processes, overcoming the difficulties in traditional composite coating fabrication. The coating exhibits excellent anti-fouling properties in complex sample fluids and can be used to fabricate functionalized electrodes for biosensing applications.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Biochemical Research Methods
Patrick Severin Sfragano, Serena Pillozzi, Gerolama Condorelli, Ilaria Palchetti
Summary: In order to address cancer-related issues, the biosensing field is constantly searching for new biomarkers that can be non-invasively analyzed. Extracellular vesicles (EVs) have emerged as promising biomarkers that can provide direct information on diseases. However, there is a lack of practical and effective quantitative procedures for EV detection due to difficulties in the pre-analytical and analytical phases. This review aims to provide a comprehensive overview of recent progress in EV detection using electrochemical and photoelectrochemical biosensors.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Xianda Han, Zhigang Yu, Yangkejia Xiang, Fengqin Li, Xiaoping Tan, Lan Xu, Guiling Zhang
Summary: An electrochemical fabrication method of Ru[bpy](3)(2+)@Cu-MOF(-SH)/Au was developed, and the proposed interface was characterized using photo/electrochemical information and validated with a DNA-based biosensing assay. The fabricated interface showed a dual signal response in both photochemistry and electrochemistry. The effects of parameter variables on the interface response were investigated, and the results followed an interference effect mechanism. Under optimized conditions, the interface exhibited high sensitivity with a low detection limit. The correlation between the interface fabrication and response performance was validated using both electrochemical and electrochemiluminescence imaging methods.
APPLIED SURFACE SCIENCE
(2022)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
